Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 138,665 C→A 6.2% intergenic (+32/+170) cueO → / ← gcd multicopper oxidase (laccase)/glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913138,6650CA 6.2% 314.3 / 4.2 103intergenic (+32/+170)cueO/gcdmulticopper oxidase (laccase)/glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (43/52);  new base A (5/2);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGT  >  NC_000913/138518‑138813
                                                                                                                                                   |                                                                                                                                                    
tctgCGAAGTGCTGGTGATGTTTTATCACGATGCTCCGTAATTACTTGCTTATATGGCGCACTGGCATCTGCTTGAGGATGAAGATACGGGGATGATGTTAGGGTTTACTGTTTAAATAGACCGTATTCGGAAATATGCCCGGAtctctc                                                                                                                                                    <  2:1200660/147‑1 (MQ=255)
 cAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATCAAGATACGGGGATGAAGTTAGGGTTAACGGTATAAAAAGACAGTATTCGGAAATATGCACGGATCTCTcc                                                                                                                                                   >  1:1294483/1‑150 (MQ=255)
      aaGTGCTGGTGAATTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATTATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCa                                                                                                                                              <  2:506514/150‑1 (MQ=255)
      aaGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCa                                                                                                                                              <  2:908644/150‑1 (MQ=255)
       aGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAGAGATCCGGGCAt                                                                                                                                             <  1:688566/150‑1 (MQ=255)
         tGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTa                                                                                                                                           <  2:776187/150‑1 (MQ=255)
          gCTGGTGAAGTTTAATCAAGATGCACCGAAATAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACTGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTaa                                                                                                                                          <  2:509177/150‑1 (MQ=255)
             ggTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGTGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTTTTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACt                                                                                                                                       <  1:750910/150‑1 (MQ=255)
             ggTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACt                                                                                                                                       >  1:149029/1‑150 (MQ=255)
             ggTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAGAGATCCGGGCAtatttcc                                                                                                                                       <  2:331145/150‑7 (MQ=255)
                taaGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgat                                                                                                                                    <  1:1221372/149‑1 (MQ=255)
                gAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgat                                                                                                                                    <  1:1800142/150‑1 (MQ=255)
                  aGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATAAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgatga                                                                                                                                  >  1:715062/1‑150 (MQ=255)
                   gTTTAATCACGATGTACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGAc                                                                                                                                 >  1:331488/1‑150 (MQ=255)
                   gTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGAc                                                                                                                                 >  2:635057/1‑150 (MQ=255)
                   gTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGAc                                                                                                                                 >  2:641866/1‑150 (MQ=255)
                   gTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGAc                                                                                                                                 >  1:1501648/1‑150 (MQ=255)
                      tAAACACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTAGGAAATATGCCCGGATCTCTCAGGGCATTAAACTGATGACaaa                                                                                                                              >  2:1604923/1‑150 (MQ=255)
                        aTCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTCGAGCATGAAGATACGGGGATGATGTAAGGGTATACGCTATAAAAAGACCGTATTCGGAAATATGCCCGGATCACTCCGCGCATTAAACTGATGACAAACg                                                                                                                            >  1:299276/1‑150 (MQ=255)
                                     gAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGa                                                                                                               >  2:1287890/1‑150 (MQ=255)
                                        aGACCATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATgc                                                                                                            <  2:1599766/150‑1 (MQ=255)
                                        aGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATgc                                                                                                            <  2:542081/150‑1 (MQ=255)
                                         gAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATgcg                                                                                                           >  1:787437/1‑150 (MQ=255)
                                           aCATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCt                                                                                                         >  1:1411500/1‑150 (MQ=255)
                                              tGCTTATATGGCGCACTGCCATCTGCTGGAGCAGGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTAGGAAATATGCCCGGATCTATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACg                                                                                                      >  2:966949/1‑150 (MQ=255)
                                               gCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGc                                                                                                     >  2:1656370/1‑150 (MQ=255)
                                               gCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGc                                                                                                     >  2:350946/1‑150 (MQ=255)
                                               gCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGc                                                                                                     <  1:9119/150‑1 (MQ=255)
                                               gCTTATATGGAGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGc                                                                                                     <  2:1654910/150‑1 (MQ=255)
                                                     atGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCa                                                                                               <  2:1677922/150‑1 (MQ=255)
                                                       ggCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGCCCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAgg                                                                                             >  2:245082/1‑150 (MQ=255)
                                                       ggCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAgg                                                                                             <  1:549773/150‑1 (MQ=255)
                                                        gcgcACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGc                                                                                            <  2:824417/150‑1 (MQ=255)
                                                        gcgcACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGAGGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGc                                                                                            <  1:215685/150‑1 (MQ=255)
                                                           cACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGCCCGTATTCGTAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACCCAAATCTGCCTGATGCGCTACGCTTATCAGACCTa                                                                                         >  1:950952/1‑150 (MQ=255)
                                                            aCTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                        <  1:455915/150‑1 (MQ=255)
                                                            aCTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                        <  1:512719/150‑1 (MQ=255)
                                                               gCCATCTTCTGGAGCATGAAGATACGGGGAAGATGTTAGGGAATACGGTAAAAAAACAACGTATTAGGAAATATGCCCGGATCTGTCCCGGCAGAAAACTGATGACAAACGCAAATCTTCCTGAAGCGCTCAGCTTATCAGGCCTACGCt                                                                                     >  2:1378499/1‑150 (MQ=255)
                                                               gCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCt                                                                                     <  1:615092/150‑1 (MQ=255)
                                                                ccATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTa                                                                                    >  1:497617/1‑150 (MQ=255)
                                                                  ttCTGATGGAGCATTAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGc                                                                                  <  1:886797/149‑1 (MQ=255)
                                                                  aTCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGc                                                                                  <  2:1154633/150‑1 (MQ=255)
                                                                  aTCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGc                                                                                  <  1:171534/150‑1 (MQ=255)
                                                                  aTCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGc                                                                                  <  1:537981/150‑1 (MQ=255)
                                                                     tgctgGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCtgt                                                                               >  1:518744/1‑150 (MQ=255)
                                                                      gctgGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGTTGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCtgtg                                                                              >  2:1233023/1‑150 (MQ=255)
                                                                      gctgGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCtgtg                                                                              <  1:1481566/150‑1 (MQ=255)
                                                                       ctgGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGc                                                                             <  2:829499/150‑1 (MQ=255)
                                                                           aGCATGAAGATTCGGGGATGTTGTTATTGTTTAGGGTTTAAATAGACAGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAATCGCAAATCTTCCTTATGCGCTACGCTTATCAGGCCTACTCTAGCTGTGCAATc                                                                         <  2:1411539/150‑1 (MQ=37)
                                                                            gCATGAAGTTACGGGGATGATGTTAGGGATTACGGTATAAAAAGAGCGTATTCGGAAATATGCCCGGTTCTCTCCTGGCATTATACTGATGACTATCGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  2:1046289/150‑1 (MQ=38)
                                                                            gCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  2:570220/150‑1 (MQ=255)
                                                                            gCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  2:440494/150‑1 (MQ=255)
                                                                            gCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  1:1080570/150‑1 (MQ=255)
                                                                            gCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  1:243789/150‑1 (MQ=255)
                                                                            gCAGGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                        <  2:93342/150‑1 (MQ=255)
                                                                              aTGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCAt                                                                      >  2:1809710/1‑150 (MQ=255)
                                                                              aTGAAGATACGGCGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGATACGCTTATCAGGCCTACCCTAGCTGTGCAATCCAt                                                                      >  2:1090354/1‑150 (MQ=255)
                                                                               tGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGACCTACGCTAGCTGTGCAATCCAtt                                                                     <  2:1619305/150‑1 (MQ=255)
                                                                                 aaGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACTCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGa                                                                   >  1:1121937/1‑150 (MQ=255)
                                                                                   gATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGAtt                                                                 >  2:1437651/1‑150 (MQ=255)
                                                                                       cGGGGATGATTTTAGTGGTTACGGTATAAAAAGACCTTATTCGGAAATATGCCCGGAGCTCTCCGGGCATTAAACTGATGAAAAACGCAAATCTTCCTGATGCGCTACTCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGc                                                             <  2:108018/150‑1 (MQ=255)
                                                                                       cGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGc                                                             >  2:472148/1‑150 (MQ=255)
                                                                                           gatgatGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACaa                                                         >  1:839150/1‑150 (MQ=255)
                                                                                            atgatgTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAAt                                                        <  2:1045477/150‑1 (MQ=255)
                                                                                                  ttAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTa                                                  <  2:782291/150‑1 (MQ=255)
                                                                                                  ttAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTa                                                  >  2:671153/1‑150 (MQ=255)
                                                                                                  ttAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTa                                                  <  1:1565735/150‑1 (MQ=255)
                                                                                                   tAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAg                                                 <  1:1211517/150‑1 (MQ=255)
                                                                                                     gggTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGc                                               <  1:1101349/150‑1 (MQ=255)
                                                                                                     gggTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGc                                               <  2:384173/150‑1 (MQ=255)
                                                                                                      ggTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCt                                              >  1:178710/1‑150 (MQ=255)
                                                                                                       gTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAACTCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCAATGATTTTGCACAATATTGTAGGCTg                                             >  1:283379/1‑150 (MQ=255)
                                                                                                          tACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGAt                                          >  1:230875/1‑150 (MQ=255)
                                                                                                             ggTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAg                                       >  1:945814/1‑150 (MQ=255)
                                                                                                               tataAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGc                                     >  1:131586/1‑150 (MQ=255)
                                                                                                                ataAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGCCAAACGCAAATCTGCCTTATGCGCTACGCTTATCAGGCCTACGCTAGCTGAGCAATCCATTGATTTTGCACATTATTGTAGGCTGGATAAGGCg                                    >  1:284599/1‑150 (MQ=38)
                                                                                                                   aaaaGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTc                                 >  1:395675/1‑150 (MQ=255)
                                                                                                                   aaaaGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTc                                 >  2:570864/1‑150 (MQ=255)
                                                                                                                      aGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcg                              <  1:1595198/150‑1 (MQ=255)
                                                                                                                      aGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcg                              <  2:1681947/150‑1 (MQ=255)
                                                                                                                      aGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcg                              <  1:1753303/150‑1 (MQ=255)
                                                                                                                        aCCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgcc                            <  1:1864672/150‑1 (MQ=255)
                                                                                                                         ccGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgccg                           <  1:1509153/150‑1 (MQ=255)
                                                                                                                          cGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgccgc                          >  2:692276/1‑150 (MQ=255)
                                                                                                                           gTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCa                         <  2:1319495/150‑1 (MQ=255)
                                                                                                                            tATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCAt                        >  1:1841102/1‑150 (MQ=255)
                                                                                                                               tCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCg                     >  1:736409/1‑150 (MQ=255)
                                                                                                                                 ggAACTATGCACGGAGCTCTCCGGGCATTAAACTGTAGACAAACGCAAATCTGCCTGAGGCGCTACGCGTAGCAGTCCTTCGCTAGCTGGGCAATCCATTGAGGTTGCACAATGTTGTAGGCGGGACAAGGCGTTCACGCCGCATCCGGc                   <  1:1391586/150‑1 (MQ=21)
                                                                                                                                 ggAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGc                   <  1:1121221/150‑1 (MQ=255)
                                                                                                                                  gtttaatGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCa                  >  1:1635581/6‑150 (MQ=21)
                                                                                                                                   aaaTATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCAt                 >  2:1586654/1‑150 (MQ=255)
                                                                                                                                    aaTATGCACGGAGCTCTCCGGGCAGTAAACTGAAGACAAACGCAAAGCTGCAAGATGAGCTACGCTTCTCAGGCCTACGCTAGCTGGGCAATCCATTGATTTTGCCCAATTTTGTATGCTTGATAAGGCGTTCACGCCGCATCCGGCATg                <  1:1317321/150‑1 (MQ=21)
                                                                                                                                      aatGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTCTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaa              >  1:1215734/2‑150 (MQ=21)
                                                                                                                                       atGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaac             >  1:711840/1‑150 (MQ=255)
                                                                                                                                       atGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTCCACAATGTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaac             >  1:1636982/1‑150 (MQ=21)
                                                                                                                                          cccGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac          >  2:186688/1‑150 (MQ=255)
                                                                                                                                            cGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAcgc        <  2:138913/150‑1 (MQ=255)
                                                                                                                                            cGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAcgc        <  1:245082/150‑1 (MQ=255)
                                                                                                                                                  tctcCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGt  >  2:53090/1‑150 (MQ=255)
                                                                                                                                                  tctcCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGt  >  1:1356815/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGT  >  NC_000913/138518‑138813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: