Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,830,065 G→C 7.5% P234R (CCT→CGT) ‡ astA ← arginine succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,0650GC 7.5% 308.6 / 11.7 102P234R (CCT→CGT) ‡astAarginine succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (42/52);  new base C (6/2);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 105 total aligned reads displayed.

GCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAA  >  NC_000913/1829916‑1830214
                                                                                                                                                     |                                                                                                                                                     
gcgcAGGCTGCCCTTCTGCCACGTCCACCCGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCCCGCGTCGGCCCACCGGCACAGATGGCGCTATAGTTACGGGAGCGAAAACCGTCTGTCTCCAGCACCGCGCGGGCAg                                                                                                                                                       >  2:7286/1‑150 (MQ=255)
  gcAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCTCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgc                                                                                                                                                     >  2:1600511/1‑150 (MQ=255)
   cAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTCCGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgcg                                                                                                                                                    <  2:849637/150‑1 (MQ=255)
    aGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgcgc                                                                                                                                                   <  1:1429812/150‑1 (MQ=255)
     ggCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGcgcg                                                                                                                                                  <  2:1825355/150‑1 (MQ=255)
        tGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGtt                                                                                                                                               <  2:744686/150‑1 (MQ=255)
          cccTTCTGCCACTTCCACCAGCCGACTATTACGGATGGCGCGCACGCGGTCGATGTCCCACTCACGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgct                                                                                                                                             <  1:1715774/150‑4 (MQ=255)
          cccGTCTGCCACTTCCACCAGCCGACTTTTACGCAGGGCGCGCACGCGGTCGATGTCCCACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCACCACCGCGCCTGCCCGCGCGGtgct                                                                                                                                             <  1:462459/150‑4 (MQ=255)
           ccTTCTGCCACTTCCCCCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCGTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTGTGc                                                                                                                                            >  1:599810/1‑150 (MQ=255)
            cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATTTAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCg                                                                                                                                           <  2:654015/150‑1 (MQ=255)
            cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGGCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCg                                                                                                                                           <  2:1830803/150‑1 (MQ=255)
             ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                                          <  2:236184/150‑1 (MQ=255)
               cagCCACTTCCCCCAGCCGACTTTTACGGAGGGCGCGCACGCGGGCGAAGGCACACTCCAGCGGCGGCCCACCGTCAAAGATGTCGATAAAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAt                                                                                                                                        <  1:1502934/148‑1 (MQ=255)
               cTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACCCTCACGCGTCGGCCCCCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAt                                                                                                                                        >  2:1397789/1‑150 (MQ=255)
               cTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGGCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAt                                                                                                                                        <  1:641232/150‑1 (MQ=255)
               cTGCCACTTCCACCAGCCGACTTTTACGGAAGGCGCGCACGCGGGCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAt                                                                                                                                        <  1:680233/150‑1 (MQ=255)
                    aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                                   >  1:660562/1‑150 (MQ=255)
                    aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCATCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGGTTGCGGATGTAcc                                                                                                                                   >  2:1299867/1‑150 (MQ=255)
                     cTTCCACCCGCCGACTTTTTCGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGGCGGCCCGCCGTCATAGATGTCGATATAGGTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCt                                                                                                                                  >  2:68271/1‑150 (MQ=255)
                           atggCCGATTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGa                                                                                                                            >  1:680482/4‑150 (MQ=255)
                             aGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                                                                                                                          <  2:1585261/150‑1 (MQ=255)
                             aGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                                                                                                                          <  2:746610/150‑1 (MQ=255)
                              gCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGAGATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGCCCGATgc                                                                                                                         >  1:932365/1‑149 (MQ=255)
                                cGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACg                                                                                                                       >  2:650618/1‑150 (MQ=255)
                                cGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACg                                                                                                                       <  1:1715590/150‑1 (MQ=255)
                                       tACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTggg                                                                                                                >  2:658854/1‑150 (MQ=255)
                                                cgcgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGTCTTCCTggg                                                                                                       >  1:1521988/1‑150 (MQ=255)
                                                cgcgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTggg                                                                                                       <  1:885124/150‑1 (MQ=255)
                                                  cgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTAGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAt                                                                                                     >  2:497038/1‑150 (MQ=255)
                                                   gcACGCGGGCGATGGCACACTCAAGCGTCGGCCCACCGTCAAAGATGCCGAAATAGATACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATa                                                                                                    <  2:1785135/150‑1 (MQ=255)
                                                    cccgcgGTCGACGTCACACTCTAGCGTCGGCCCACCGGCAAAGATGTCGATAAAGTTACGGTTGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGGGCGGTTTGCGGATGTACCTGACCGCTGACGTCCTGGGCTTCCTGGGATaa                                                                                                   <  2:700067/148‑1 (MQ=255)
                                                      cgcgGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaa                                                                                                 <  2:1312308/150‑1 (MQ=255)
                                                             gATGTCTCACTCACGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGTCTTCCTGGGATAAAAAGTGGGt                                                                                          <  1:296041/150‑1 (MQ=255)
                                                                  cacaCTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGa                                                                                     >  1:966271/1‑150 (MQ=255)
                                                                       gccaGCGTCGGCCCACCGTCAAAGATGCCGATATCGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGa                                                                                <  2:1375722/147‑1 (MQ=255)
                                                                           gCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGtt                                                                            <  1:1053101/150‑1 (MQ=255)
                                                                             gTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTc                                                                          <  1:377733/150‑1 (MQ=255)
                                                                             gTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTc                                                                          <  1:497038/150‑1 (MQ=255)
                                                                               cGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCgg                                                                        <  2:1815959/150‑1 (MQ=255)
                                                                                 gCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGTATAAAAAGTGGGTATAGATCGGATGTTTCGGCa                                                                      <  1:157855/150‑1 (MQ=255)
                                                                                 gCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATATAAAGTGGGTATAGATCGGAGGTTTCGGcc                                                                      >  1:1797106/1‑149 (MQ=255)
                                                                                   ccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATc                                                                    >  2:175685/1‑150 (MQ=255)
                                                                                   ccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATc                                                                    >  2:686450/1‑150 (MQ=255)
                                                                                     aCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAg                                                                  <  1:1281769/150‑1 (MQ=255)
                                                                                      ccGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGt                                                                 >  2:1350927/1‑150 (MQ=255)
                                                                                        gTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTc                                                               <  2:1557288/150‑1 (MQ=255)
                                                                                        gTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTc                                                               >  2:883841/1‑150 (MQ=255)
                                                                                           aaaGATGTCGATATAGTTACGGTAGCGACAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                            >  1:1663834/1‑150 (MQ=255)
                                                                                           aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCTCCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                            <  1:934225/150‑1 (MQ=255)
                                                                                           aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTTGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                            <  2:1669260/150‑1 (MQ=255)
                                                                                           aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                            <  1:1302304/150‑1 (MQ=255)
                                                                                           aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                            <  2:669287/150‑1 (MQ=255)
                                                                                            aaGATGGCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCa                                                           <  2:1435791/150‑1 (MQ=255)
                                                                                             aGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCTCGGTTTTCTGATGTACCTGACCGATGACGGCCTGTGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCaa                                                          <  1:225850/150‑1 (MQ=255)
                                                                                             aGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCaa                                                          <  2:1273093/150‑1 (MQ=255)
                                                                                                tGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaa                                                       >  1:1663200/1‑150 (MQ=255)
                                                                                                tGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaa                                                       <  2:776931/150‑1 (MQ=255)
                                                                                                 gTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaaa                                                      <  2:1172923/150‑1 (MQ=255)
                                                                                                   cGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAACTg                                                    >  2:891224/1‑150 (MQ=255)
                                                                                                    gATATAGTTACGGTAGCGAAAACCTACTTTCACCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGACCTGGGCTTCCTGGGATAAAAAGGGGGTATAGCTCGGATGTATCGGCAACATTTCTGCAATAAATGc                                                   >  2:1015825/1‑150 (MQ=255)
                                                                                                     atatAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCCCCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGcc                                                  <  2:1049375/150‑1 (MQ=255)
                                                                                                        taGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGAAGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCttt                                               <  2:655730/150‑1 (MQ=255)
                                                                                                          gTTACGGTTGCGCAAACCTTCTTTCTCCAGCAACGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGTCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGCTGGTTCGGCATCAGTTCAGCAATAAATGCCTTTTg                                             <  2:1207472/150‑1 (MQ=255)
                                                                                                           ttACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGc                                            <  1:190668/150‑1 (MQ=255)
                                                                                                               ggTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCgg                                        >  2:924033/1‑150 (MQ=255)
                                                                                                               ggTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCgg                                        <  2:680482/150‑1 (MQ=255)
                                                                                                               ggTAGCGAAAACCATCTTTCTCCAGCACCGCGCTGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTGCCTGGGATAAAAATTGGGTATAGATCTTATGTTTCGGCATCATTTCTGCAATAAATGCCTTTTGCCCgg                                        <  2:226831/150‑1 (MQ=255)
                                                                                                                    cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGTTAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCg                                   >  2:22479/1‑150 (MQ=255)
                                                                                                                    cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCg                                   >  1:695195/1‑150 (MQ=255)
                                                                                                                      aaaaCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCa                                 <  1:1034248/150‑1 (MQ=255)
                                                                                                                          ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                             >  1:1426124/1‑150 (MQ=255)
                                                                                                                          ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                             >  1:1702067/1‑150 (MQ=255)
                                                                                                                          ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                             <  2:523216/150‑1 (MQ=255)
                                                                                                                            ttctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaa                           <  2:1776314/150‑1 (MQ=255)
                                                                                                                            ttctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaa                           <  1:699/150‑1 (MQ=255)
                                                                                                                             tctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGTGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaaa                          <  1:1512324/150‑1 (MQ=255)
                                                                                                                              ctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAAt                         >  1:105591/1‑150 (MQ=255)
                                                                                                                              ctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAAt                         >  2:48046/1‑150 (MQ=255)
                                                                                                                              ctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTGTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAAt                         >  2:53496/1‑150 (MQ=255)
                                                                                                                               tttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATc                        >  1:741948/1‑150 (MQ=255)
                                                                                                                               tttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATc                        <  1:650618/150‑1 (MQ=255)
                                                                                                                                ttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCg                       >  1:24867/1‑150 (MQ=255)
                                                                                                                                  ctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgc                     >  2:1154074/1‑150 (MQ=255)
                                                                                                                                   tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGTTGTACCTGACCGATGCGGGCCTGGGCTTCCTGGGAGACAAAGTGGGTATAGAGCGGATGTTTCGGCAGCAGTTCTGCTATAAGTGCCTGTTGCCCGGTGCCGCAGAGAAATgcggcg                    >  2:127211/1‑150 (MQ=255)
                                                                                                                                   tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTTTAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGGAGAGAAAATCGgcg                    >  1:733260/1‑150 (MQ=255)
                                                                                                                                   ccgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATAGGATGTTTCGGCATCAGATCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg                    >  1:914915/7‑150 (MQ=255)
                                                                                                                                    cgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc                   >  1:1645072/6‑150 (MQ=255)
                                                                                                                                    cgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc                   >  2:1830468/6‑150 (MQ=255)
                                                                                                                                    ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGGATAGATCGGATGTTTCGTCATCAGTTCTGCAATAAACGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc                   >  2:360575/1‑150 (MQ=255)
                                                                                                                                     cAGGACCGCGAGGGAAGGCGCGGTTTGTGGATGTTCCTGACGGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGcgcg                  <  1:99080/150‑1 (MQ=255)
                                                                                                                                      caaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAGAAAAAGTGGGTATAGATCGGTTGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCgg                 >  1:339576/4‑150 (MQ=255)
                                                                                                                                      aGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAGAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCgg                 >  1:778945/1‑150 (MQ=255)
                                                                                                                                       gCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc                >  2:564284/1‑150 (MQ=255)
                                                                                                                                       aaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc                >  2:1400328/3‑150 (MQ=255)
                                                                                                                                                  gCAGGCGCGGTTTGCGGCTGTACCTGCCCGATGCCGTCCTGGGCTTCCTGGGATAACAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATc     <  1:1206087/150‑1 (MQ=255)
                                                                                                                                                  gCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATc     <  1:625101/150‑1 (MQ=255)
                                                                                                                                                   cAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCg    <  2:41746/150‑1 (MQ=255)
                                                                                                                                                   cAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCg    <  1:1600511/150‑1 (MQ=255)
                                                                                                                                                     ggCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGaa  >  2:724264/1‑150 (MQ=255)
                                                                                                                                                     ggCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGAGGTTTCGGAATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCTaa  >  2:739612/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAA  >  NC_000913/1829916‑1830214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: