| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 291 | A→T | 5.8% | intergenic (+36/‑46) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 291 | 0 | A | T | 5.8% | 306.7 / 4.7 | 122 | intergenic (+36/‑46) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (65/46); new base T (3/5); total (70/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCAC > NC_000913/143‑440 | taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCTTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGAc < 2:1282877/150‑1 (MQ=255)taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGAc < 1:127395/150‑1 (MQ=255)taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGAc > 1:185838/1‑150 (MQ=255) aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACa > 2:889000/1‑150 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCCCCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCggg > 1:221546/1‑150 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCggg < 2:322803/150‑1 (MQ=255) gcACAGACAGATAAATATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTAGCACCACCATCACCATTTCCTCAGGTAACGGTGCGGGCTGACGCGTAAAGGAAACACAG‑AAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑t < 1:1291041/150‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCCCCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑t < 1:1349953/150‑1 (MQ=255) cacaGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tt > 1:120608/1‑150 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttt < 1:882304/150‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttt < 1:400825/150‑1 (MQ=255) agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttt < 2:800754/150‑1 (MQ=255) cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCCTTAGCACCACCATTACCACCACCATCACCATTACCACCCGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tttttttt < 1:1255852/150‑1 (MQ=255) taTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAGACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTc < 1:1199767/149‑1 (MQ=255) gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTc > 1:1088351/1‑150 (MQ=255) aTAAAAATTACAGAGTACACAACCTCCATGAAACGCCTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCg < 2:1648594/150‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAACCGCATTATCACCACCATTACCCCCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttttttctgtg < 2:916601/150‑6 (MQ=38) aaaaaTTACATAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGAc < 2:931587/150‑1 (MQ=255) aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGAcc > 2:337736/1‑150 (MQ=255) aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAAGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGAcc > 2:1212455/1‑150 (MQ=255) aaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCa > 1:764344/1‑150 (MQ=255) aCAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGt > 2:1304255/1‑150 (MQ=255) agTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTaaaa > 1:940211/1‑148 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTAACACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGt > 2:1127119/1‑150 (MQ=255) acaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaaca > 2:173051/1‑150 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGTAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACACCCa > 2:68383/1‑150 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa > 2:629272/1‑150 (MQ=255) cATGAAACGCATTAGCCCCACCATTACCACCACCATCACCAATACCACAGGTAACGGTGCGGGCTGACGCGGACAGGAAACACAG‑AAAAAAGCCCGCACCTGACC‑GGCCGGGC‑TTGTGTTTGCCACCAAAGGTAACGAGGTAACACCCAt > 1:345543/1‑150 (MQ=255) cATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAt > 2:1042905/1‑150 (MQ=255) cATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAt > 2:794226/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAACCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg < 2:263193/150‑1 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGCCCAAAGGTAACGCGGTAACACCCATGc > 1:498655/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg < 1:17970/150‑1 (MQ=255) cGCATTAGCACCACCATTACCACCCCGATCACCATTACCACAGGTATCTGTGCGGGCTGACGCGTACAGGAAACCCAG‑AATAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg < 2:1046502/150‑1 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑GGTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAgtgt > 2:605782/1‑150 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAGGAGGTAACAACCATGCGAGTGtt > 1:1302419/1‑150 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 2:1524718/150‑1 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt > 2:1540879/1‑150 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 1:794226/150‑1 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAGGCGAGTGtt > 1:646182/1‑150 (MQ=255) atttcccaccacCAATAACACCACCTTGACCATTACCTCTGGTAAGGGTGCGGTCTGACGCGTACAGGAAACTCAG‑AAAAACCCCCTGACCTGACA‑TTGCGGTG‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 2:1747370/144‑1 (MQ=255) aGCACCACGATTACCACCACTATCACCATTACCACAGGTAAGGGTGCGGGCTGACGCGTACTGGAAACACTG‑AAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg < 2:986249/150‑1 (MQ=255) gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAAGCGAGTGTTGAAGt > 1:468653/1‑150 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:52621/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg > 1:1189211/1‑150 (MQ=255) tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc < 1:1304255/150‑1 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc > 1:1661706/1‑150 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc > 2:1858619/1‑150 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTTACGCGGTAACAACCCTGCGAGTGTTGCAGTTCGGCGGttactca > 2:1665685/1‑144 (MQ=255) gcccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TGTTTTTTTCGCCCAAAGGTAACGAGGTAACACCCATGCGAGTGTTGAAGTTCGGCGGTACCTCAGTg > 1:1561072/6‑150 (MQ=255) gcccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg > 1:1096718/6‑150 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg < 1:385351/150‑1 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAACAGTg > 2:1789685/1‑150 (MQ=255) cccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGTGTTTTGAAGTGCGGCGGTACATCAGTgg > 2:647151/5‑150 (MQ=255) ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg < 2:185838/150‑1 (MQ=255) ccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc > 2:778383/4‑150 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc > 2:875795/1‑150 (MQ=255) atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAGGAGGTAACAACCATGCGTGTGTTGAAGTTCGGCGGTACATCAGTGGCa > 2:68171/1‑150 (MQ=255) atcaccatAACCACAGGTAACGGGGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa < 2:976253/150‑1 (MQ=255) catTACGACAGGGTACGGTGCGGGCTGACGGGTTGAGGAAACACAG‑AAAAAAGCCCGCAGCTGCCA‑GTGAGGGC‑TTTTTTTTTCGACATAAGGTAACGAGGTAACAACCATGCGAGGGTTGAAGTTCGGCGCTGCCTCAGTGGCAAATGc > 2:103694/1‑150 (MQ=255) catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCGGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc < 2:903786/150‑1 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGTGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAATTGCAGAAc > 2:162898/1‑150 (MQ=255) caGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtt > 1:102143/1‑150 (MQ=255) gTAACGGTTCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 1:1224505/150‑1 (MQ=255) tAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTAGACCAAAGGTAACGAGGTTAAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCt > 1:1841539/1‑150 (MQ=255) aaCGGTGCGGGCTGACGCGTAAAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg < 1:227582/150‑1 (MQ=255) aaCGGGGCGGGCTGCCGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg < 2:746515/150‑1 (MQ=255) aCGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGc < 1:540336/150‑1 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg < 2:1509070/150‑1 (MQ=255) gTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCTAATGCAGAACGTTTTCTGCgtg < 1:831456/150‑1 (MQ=255) gTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtg < 1:230020/150‑1 (MQ=255) gggCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc < 2:1245147/150‑1 (MQ=255) cTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGGCGa > 1:1566791/1‑150 (MQ=255) cTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 1:173051/150‑1 (MQ=255) gACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGat > 1:1023890/1‑150 (MQ=255) gcgTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc < 1:1326494/150‑1 (MQ=255) gTACAGGAAACACAG‑AAAAAATCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACTAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:1789309/150‑1 (MQ=255) tACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 1:1854253/150‑1 (MQ=255) cAGGAAACACAGAAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa > 2:1227994/1‑150 (MQ=255) cAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTAGACCAAAGGTAAAGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATAAGTGGCAAATGCAGCACGTTTTCTGCGTGTTGCCGATATTCTGGaa > 1:939994/1‑150 (MQ=255) cAGCAAACACAT‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 2:1824584/150‑1 (MQ=255) gAAACACAG‑AAAAAAGCCCGCACCTGTCA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc < 1:1653642/150‑1 (MQ=255) cacaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATg > 2:663383/1‑150 (MQ=255) cacaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAAGAGGTAACACCCATGCGTGTGTTGAAGTTCGGCGTGACTTCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATTTTGTGGAAAGCAATg > 2:336141/1‑150 (MQ=255) acaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTATGCGTGTTGCCGATATTATGGAAAGCAATGc > 2:929723/1‑150 (MQ=255) gaaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 1:717114/5‑150 (MQ=39) aaaaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:453233/1‑150 (MQ=255) aaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTCGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca > 2:363427/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:102143/150‑1 (MQ=255) aGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 2:1625000/1‑150 (MQ=255) cccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGCCCAAAGGTAACGAGGTAACAACCATGCGTGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggc > 2:708835/1‑150 (MQ=255) cccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggccggggc > 2:214490/1‑150 (MQ=255) cccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggc > 1:1151506/1‑150 (MQ=255) cGCACCTGGCA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt < 2:580333/104‑1 (MQ=255) cGCACCTGGCA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 1:580333/1‑104 (MQ=255) cGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATAAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag > 1:986301/1‑150 (MQ=255) ccTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg > 1:1005940/1‑150 (MQ=255) tGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGcc > 2:1424862/1‑150 (MQ=255) gACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCa > 2:74465/1‑150 (MQ=255) aCA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCAc < 1:1127119/150‑1 (MQ=255) | TAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCAC > NC_000913/143‑440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |