Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 139,128 G→A 5.7% P700S (CCG→TCG)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913139,1280GA 5.7% 319.9 / 3.7 104P700S (CCG→TCG) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (37/61);  new base A (3/3);  total (40/64)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACA  >  NC_000913/138979‑139276
                                                                                                                                                     |                                                                                                                                                    
tAATCGACCCTGCCACTGTTGTTCACCGGTGCTCCGGTTGTAAGCGCGCAGGTCGATAGCTGCCGTAGCGGCGATAAACAGCACGTTCCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCgg                                                                                                                                                      <  2:265289/150‑1 (MQ=255)
tAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCTCCCCGCATCGTCATACCCATATTGAACGGCACCGGAACCgg                                                                                                                                                      >  1:76957/1‑150 (MQ=255)
  aaCGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCa                                                                                                                                                    <  2:827469/150‑1 (MQ=255)
      aCCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCgg                                                                                                                                                <  1:469512/150‑1 (MQ=255)
           gCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACg                                                                                                                                           >  1:594040/1‑150 (MQ=255)
            ccACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGGGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGTAACgg                                                                                                                                          <  2:442838/150‑1 (MQ=255)
             cacaGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGc                                                                                                                                         >  1:810688/1‑150 (MQ=255)
             cacaGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGc                                                                                                                                         <  2:861744/150‑1 (MQ=255)
             cacaGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGACATCGGGAACGGc                                                                                                                                         <  1:1027051/150‑1 (MQ=255)
              acaGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCCCCGGAACCGACATCGGGACCGGCa                                                                                                                                        >  2:386604/1‑150 (MQ=255)
              acaGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATAATGAACGGCACCGGAACCGGCATCGGGAACGGCa                                                                                                                                        >  1:1141095/1‑150 (MQ=255)
                 gTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATAc                                                                                                                                     <  2:430531/150‑1 (MQ=255)
                   ttttCACCGTTGCTCATGTTGTAAGCGCGAAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAAACGGCATCGGGAACGGCATACTg                                                                                                                                   >  2:107719/1‑150 (MQ=255)
                   ttttCACCGTAGCTCATGTTGTAAGCGCGCAGGTAGATATCTGCCGTAGAGGCGATAAACAGCAAGTTACCCGACGTGGAGATTGGCCCGCCCAGCATCGGCATACCCAAATACAAAGGCACCGGATCCGACATCGGGAACGGCATAcag                                                                                                                                   >  1:202596/1‑148 (MQ=255)
                       cACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGATCGGCACCGGCACCGGCATCGGGAACGGCATACTGTCCt                                                                                                                               >  2:943757/1‑150 (MQ=255)
                           gTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcgg                                                                                                                           >  1:1548656/1‑150 (MQ=255)
                           gTTGCTCATGTTGTAACCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGCAACCGGCATCGGGAACGGCATACTGTCCTgcgg                                                                                                                           <  2:109124/150‑1 (MQ=255)
                            ttGCTCATGTTGAAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCTGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggc                                                                                                                          <  1:115725/150‑1 (MQ=255)
                            ttGCTCATGGTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggc                                                                                                                          <  1:149877/150‑1 (MQ=255)
                             tGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggcg                                                                                                                         <  2:20615/150‑1 (MQ=255)
                               cTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTa                                                                                                                       >  1:1159735/1‑150 (MQ=255)
                                tCATGTTGTAAGCGCGCCGGTAGTTATCTGCCGTAGCGGCGATAACCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAc                                                                                                                      <  2:124666/150‑1 (MQ=255)
                                tCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAc                                                                                                                      <  2:1537734/150‑1 (MQ=255)
                                tCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAc                                                                                                                      <  1:1105945/150‑1 (MQ=255)
                                 cATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAcc                                                                                                                     <  1:1349487/150‑1 (MQ=255)
                                   tgttgtAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCGAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCAGCGGGAACGGCATACTGTCCTGCGGCGTACCaa                                                                                                                   <  1:343016/150‑1 (MQ=255)
                                    gttgtAAGCGCGCAGGTAGTTAGCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAAt                                                                                                                  <  1:1459678/150‑1 (MQ=255)
                                    gttctACGCGCGCAGGTAGTAATCTGCCGAAGCGGCCATAAACAGCACGTTACCCGCCGTGGAGATAGGCCCGCCCAGCATCGACATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGTGTACCaaa                                                                                                                  <  1:923419/150‑2 (MQ=255)
                                     ttgtAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATa                                                                                                                 <  1:752483/150‑1 (MQ=255)
                                      tgtAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATAc                                                                                                                <  2:1453621/150‑1 (MQ=255)
                                       gtAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAAAAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACg                                                                                                               <  1:43021/150‑1 (MQ=255)
                                         aaGCGCGCAGGTAGTTATCTGTCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTATCCTGCGGCGTACCAATACGtt                                                                                                             <  2:1260808/150‑1 (MQ=255)
                                          aGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGttt                                                                                                            <  2:648877/150‑1 (MQ=255)
                                              cgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttcttc                                                                                                        <  2:1260696/150‑1 (MQ=255)
                                              cgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttcttc                                                                                                        >  2:1833251/1‑150 (MQ=255)
                                               gcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTcc                                                                                                       <  1:427696/150‑1 (MQ=255)
                                                 ccggaGTTCTCTGCCGTAGCGGCGATAAACAGCACGATACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGCACCGGCTTCGGTAACGGCATACTGTCCTGCGGCGGACCAATACGTTTCTTCcac                                                                                                     <  2:283379/146‑1 (MQ=255)
                                                  ggTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcaca                                                                                                    <  2:438167/150‑1 (MQ=255)
                                                  ggTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcaca                                                                                                    >  1:1778252/1‑150 (MQ=255)
                                                      gTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGATCGGCAGACGGTCCTGCGGCGTCCCAATACGGTTCTGCCCcacccc                                                                                                >  1:1637752/1‑148 (MQ=255)
                                                          tCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCTTCCTGTCCTGCGGCGTACCGATACGTTTCTTCCACACCACTTCa                                                                                            >  1:115230/1‑150 (MQ=255)
                                                           cTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAt                                                                                           <  2:878710/150‑1 (MQ=255)
                                                            tGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAtt                                                                                          <  2:188573/150‑1 (MQ=255)
                                                            tGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAtt                                                                                          <  1:763880/150‑1 (MQ=255)
                                                             gCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTa                                                                                         >  1:1551546/1‑150 (MQ=255)
                                                              ccGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAg                                                                                        <  2:178495/150‑1 (MQ=255)
                                                                 gaGCGGCGATAAACAGCACGATACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCTGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGtat                                                                                     <  2:954289/149‑3 (MQ=255)
                                                                   gcggcgATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCGAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGATCGGCATACTGGCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTc                                                                                   <  1:1110361/150‑1 (MQ=255)
                                                                   gcggcgATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCCGCAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCATACACACCACTTCATTAGTTTTc                                                                                   >  1:608352/1‑150 (MQ=255)
                                                                     ggcgATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAg                                                                                 >  2:1782704/1‑150 (MQ=255)
                                                                         aTAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATcc                                                                             <  1:1020135/150‑1 (MQ=255)
                                                                          tAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGAATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCa                                                                            <  2:76957/150‑1 (MQ=255)
                                                                          aaaaCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCa                                                                            <  2:800506/149‑1 (MQ=255)
                                                                           aaaCAGCACGTTGCCCGACGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATTTTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTACCACACCACTTCATTAGTTTTCAGATCCAg                                                                           <  2:277749/150‑1 (MQ=255)
                                                                           aaaCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAg                                                                           >  2:990346/1‑150 (MQ=255)
                                                                             aCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgcg                                                                         >  2:1044245/1‑150 (MQ=255)
                                                                              cAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCCGCATCGGCATACCCATATTGACCGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCGGCGGCGTACCAAGACGATTCTTCCACACCAGATCATTTGTTGTCAGATCCGgcgc                                                                        >  2:302441/1‑150 (MQ=255)
                                                                              cAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgcgc                                                                        <  1:549643/150‑1 (MQ=255)
                                                                                gCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCg                                                                      >  1:1036591/1‑150 (MQ=255)
                                                                                 cACGTTACCCGCCGTGGAGATAGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGCACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCATCGCCga                                                                     >  2:188887/1‑150 (MQ=255)
                                                                                  aCGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCTCACCACTTCATTAGTTTTCAGATCCAGCGCCgag                                                                    <  1:107719/150‑1 (MQ=255)
                                                                                   cGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCgaga                                                                   <  2:1355457/150‑1 (MQ=255)
                                                                                     ttACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAAACGACATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTACACACCACTTCATTAGTTTTCAGATCCAGCGCCgagttt                                                                 >  1:1110424/1‑147 (MQ=255)
                                                                                       aCCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTTAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCTGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGAtata                                                               <  2:1469920/150‑1 (MQ=255)
                                                                                         ccgccgTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGTATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAAc                                                             >  1:1099139/1‑150 (MQ=255)
                                                                                                 gagaTTGGCCCGCCCAGCATCGGCATACCCTTATTGATCGGCACCGGAACCGACATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTGAGATCCAGCGCCGAGATATAACCCCATgct                                                     <  2:202596/150‑1 (MQ=255)
                                                                                                  agaTTGGCCCGACCAGCATCGGCATACCCATATTTAACGGCACAGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctg                                                    <  2:1380890/150‑1 (MQ=255)
                                                                                                    aTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctggc                                                  <  2:675026/150‑1 (MQ=255)
                                                                                                     ttGGCCAGCCCAGCATCGGCATACCCATATTGAACGTCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctggct                                                 <  1:467526/150‑1 (MQ=255)
                                                                                                      aggCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCGTGCGGCGTACCAATCCGTTTCTTCCCCACCACTTCATTAGTTTTCAGCTCCAGCGCCGAGATATCACCCCATgctgcgtg                                                >  1:392849/2‑146 (MQ=255)
                                                                                                       ggCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGt                                               <  1:1416324/150‑1 (MQ=255)
                                                                                                       ggCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGt                                               >  2:593913/1‑150 (MQ=255)
                                                                                                         cccgcccAGAATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGttt                                             <  2:594040/150‑1 (MQ=255)
                                                                                                             cccAGCATCGGCATACCCATATTGAACGGCTCCGGAACCGGCAGCGGGAACGGCATACTGTCCAGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGTTCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACAt                                         <  1:477185/150‑1 (MQ=255)
                                                                                                             cccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACAt                                         >  2:1706815/1‑150 (MQ=255)
                                                                                                             cccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACAt                                         >  2:410283/1‑150 (MQ=255)
                                                                                                             cccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACAt                                         <  2:1360985/150‑1 (MQ=255)
                                                                                                              ccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATg                                        <  1:150895/150‑1 (MQ=255)
                                                                                                              ccAGCATCGGCATACACATATTGAACGGCACCGGAACCGACATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATg                                        <  1:386604/150‑1 (MQ=255)
                                                                                                                  cATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACGAATTCGTTTCTTCCACACCCCTTCATTAGTTTTCAGCTCCAGCGCCGAGCTATAACCCCATGCTGGCTGTTTACATGGCAg                                    >  2:1153940/1‑150 (MQ=255)
                                                                                                                    tCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGAc                                  <  2:1548656/150‑1 (MQ=255)
                                                                                                                      ggCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACGTGGCAGCCCa                                >  1:1558202/1‑150 (MQ=255)
                                                                                                                        cATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCAGGCTGGCTGTTTACATGGCAGACCaaa                              >  2:1381981/1‑150 (MQ=255)
                                                                                                                          tACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATg                            >  1:496586/1‑150 (MQ=255)
                                                                                                                           aCCCATTTTGCACGGCCCCCGCACCGGCCTCGGGTACGGCAAACTGTCCTGCGGCGAACCAATACGTTTCATACACCCCACTTCAATAGCTTTCAGCTCCCGCGGCTATATATAACCCCATGCTGGCTGATTACATGGCAGACCAAATgg                           <  2:1354029/150‑1 (MQ=255)
                                                                                                                           aCCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                           <  1:1713868/150‑1 (MQ=255)
                                                                                                                           aCCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                           <  2:1704102/150‑1 (MQ=255)
                                                                                                                           aCCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATgg                           <  1:1434090/150‑1 (MQ=255)
                                                                                                                            cccATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACATTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTCCATGGCAGACCAAATGGt                          >  2:769711/1‑150 (MQ=255)
                                                                                                                                tatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACCCCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTgaga                      >  1:1048159/1‑150 (MQ=255)
                                                                                                                                tatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTgaga                      <  2:1034973/150‑1 (MQ=255)
                                                                                                                                  ttGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCAAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAgg                    >  1:1623190/1‑150 (MQ=255)
                                                                                                                                    gAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCCCTTCATTAGATTTCAGATCCAGCGCCGAGATATAACCCCAGGCTGGCTGTTTACTTGGCAGACCAAATGGTGAGAGGac                  >  2:1608213/1‑149 (MQ=255)
                                                                                                                                    gAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCACATGGTGAGAGGaa                  >  1:1719448/1‑150 (MQ=255)
                                                                                                                                     aaCGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCCCACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAAc                 >  1:1055570/1‑150 (MQ=255)
                                                                                                                                            ccGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGa          >  1:1717839/1‑150 (MQ=255)
                                                                                                                                              ggAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGc        <  1:943757/150‑1 (MQ=255)
                                                                                                                                               gAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCg       <  2:879137/150‑1 (MQ=255)
                                                                                                                                                  ccGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGa    >  1:988411/1‑150 (MQ=255)
                                                                                                                                                    ggCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGaca  >  1:1050617/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGCGCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACA  >  NC_000913/138979‑139276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑