Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,808 T→A 5.6% intergenic (+19/‑37) yeaW → / → yeaX putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,8080TA 5.6% 300.3 / 3.7 102intergenic (+19/‑37)yeaW/yeaXputative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base T (55/41);  new base A (4/2);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTAC  >  NC_000913/1885660‑1885957
                                                                                                                                                    |                                                                                                                                                     
agaGAAAGGGCTGAAAGCGCGTGGCTAGCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCGATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAttt                                                                                                                                                      <  1:304318/148‑1 (MQ=255)
aCAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAttt                                                                                                                                                      <  1:499225/150‑1 (MQ=255)
aCAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAatt                                                                                                                                                      >  1:1005371/1‑150 (MQ=255)
aCAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATAGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAttt                                                                                                                                                      >  2:185193/1‑150 (MQ=255)
   gAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCAGTTCCGAACATGGTATCGCCCAGTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTTATGACATCTGCGGCGGTATTTTcc                                                                                                                                                   >  2:726496/1‑150 (MQ=255)
         gCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGAAAATACcgccgcc                                                                                                                                             <  2:661928/150‑2 (MQ=255)
          cTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTg                                                                                                                                            <  2:1844825/150‑1 (MQ=255)
            gacaTCGCGGGGCTATCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTggg                                                                                                                                          <  1:10600/147‑1 (MQ=255)
            gAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTggg                                                                                                                                          >  1:1788602/1‑150 (MQ=255)
             aaaTCGCGTGGCTAGCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGc                                                                                                                                         <  1:450325/150‑1 (MQ=255)
              aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTTTCGCCCATTTCCATTATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGcg                                                                                                                                        >  2:851518/1‑149 (MQ=255)
              aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCt                                                                                                                                        >  1:402539/1‑150 (MQ=255)
              aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCt                                                                                                                                        >  2:1382740/1‑150 (MQ=255)
                 cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCTTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGTGCTGAt                                                                                                                                     >  2:952401/1‑150 (MQ=255)
                 cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAt                                                                                                                                     <  1:507897/150‑1 (MQ=255)
                   cgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAttt                                                                                                                                   >  1:1761589/1‑150 (MQ=255)
                    gTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAtttt                                                                                                                                  >  1:164802/1‑150 (MQ=255)
                       gCTATCGGGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGa                                                                                                                               <  1:1484374/150‑1 (MQ=255)
                        cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                              >  1:848294/1‑150 (MQ=255)
                        cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                              >  2:487074/1‑150 (MQ=255)
                         tATCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATg                                                                                                                             <  1:844768/150‑1 (MQ=255)
                             gTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGt                                                                                                                         >  1:625451/1‑150 (MQ=255)
                             gTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGt                                                                                                                         >  2:852953/1‑150 (MQ=255)
                                  cAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGc                                                                                                                    <  1:1701951/150‑1 (MQ=255)
                                     gggTGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAAt                                                                                                                 <  2:687611/150‑1 (MQ=255)
                                           atcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGa                                                                                                           >  1:1401298/1‑150 (MQ=255)
                                              atGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTa                                                                                                        >  1:9949/1‑150 (MQ=255)
                                              atGGCCGACAGTAGCGGTAGTGACATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTa                                                                                                        >  1:1019630/1‑150 (MQ=255)
                                                ggCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTTCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATc                                                                                                      <  2:53971/150‑1 (MQ=255)
                                                ggCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATc                                                                                                      <  1:251797/150‑1 (MQ=255)
                                                  ccGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCaa                                                                                                    >  1:1437919/1‑150 (MQ=255)
                                                     aCAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATATCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTAATGGAGTACAGCAAAGTAAGACTATCAAATg                                                                                                 >  1:1117299/1‑150 (MQ=255)
                                                         tAGCTGTAGTGGCATTTCCGAACATGGTATAGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTg                                                                                             <  2:86498/150‑1 (MQ=255)
                                                          aGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                            >  1:472369/1‑150 (MQ=255)
                                                          aGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                            >  1:479274/1‑150 (MQ=255)
                                                           tcGGTAGTGTCATTTCCGAACATGGTATCGCCCAATTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAAAGACATCGGCGGCGGTCTTTTCCGCCGCGGGGCTGATTGTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                           <  2:329544/149‑1 (MQ=255)
                                                           gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                           <  2:773881/150‑1 (MQ=255)
                                                            cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                          <  2:479274/150‑1 (MQ=255)
                                                            cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                          <  2:472369/150‑1 (MQ=255)
                                                            cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                          <  1:1858580/150‑1 (MQ=255)
                                                             ggTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAAGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCACACTATCAAAAGTTAGAAGt                                                                                         >  2:1862384/1‑150 (MQ=255)
                                                                  tGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAgg                                                                                    >  2:592866/1‑150 (MQ=255)
                                                                   ggCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGt                                                                                   >  1:1720378/1‑150 (MQ=255)
                                                                      aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                                >  1:595920/1‑150 (MQ=255)
                                                                      aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTAACAAATGTTTGAAGTACAGGTGat                                                                                >  2:389997/1‑149 (MQ=255)
                                                                      aTTTCCGAACATGGTATCGCAAATTTCCATAATCTGCTGGCGCAGGTGTTAAGGACTAATGCCATCGGAGGCTGTAATATCCGCCGCTGTGCTGATTTTTGATGGAGTACATCAATGTCAGCATATCAAATGTTTGAAGTACAGGTGAGc                                                                               >  2:1283130/1‑150 (MQ=255)
                                                                         tCCGAACATCGTATCGCCCATTTCCATAATCTGCTGGCGCAGGAGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCa                                                                             <  2:1645471/150‑1 (MQ=255)
                                                                          ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                            >  2:1378648/1‑150 (MQ=255)
                                                                          ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                            >  2:1694089/1‑150 (MQ=255)
                                                                          ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                            >  2:60417/1‑150 (MQ=255)
                                                                            taaCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGTTATATACCGCCGCTGGGCTGATTTTTGATGGAGAACACCAATCTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGt                                                                          <  1:103866/149‑1 (MQ=255)
                                                                            gAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACACCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAggg                                                                          >  2:1513606/1‑149 (MQ=255)
                                                                            gAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGGGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACATGTGAGCCAGGt                                                                          <  1:1759235/150‑1 (MQ=255)
                                                                             aaCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGtt                                                                         <  1:539319/150‑1 (MQ=255)
                                                                              aCATGGTATCGCCAATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTg                                                                        <  2:1697018/150‑1 (MQ=255)
                                                                               cATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACGAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACACCAATGTCAGACTAACAAAAGTTTGAAGTAAAGGTGACCCAGGTTGa                                                                       >  1:667071/1‑150 (MQ=255)
                                                                                 tGGTATCGCCCATTTCAATAATCTGCTGGCGCAGGTGTTTAAGTACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAc                                                                     <  1:928506/150‑1 (MQ=255)
                                                                                     aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                                 >  1:219750/1‑150 (MQ=255)
                                                                                      tCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCtt                                                                >  1:1325988/1‑150 (MQ=255)
                                                                                       cGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTa                                                               >  1:226071/1‑150 (MQ=255)
                                                                                       cGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTa                                                               >  1:1680710/1‑150 (MQ=255)
                                                                                        gCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                              <  1:1244000/150‑1 (MQ=255)
                                                                                        gCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                              >  2:1837583/1‑150 (MQ=255)
                                                                                          ccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCg                                                            <  2:1373916/150‑1 (MQ=255)
                                                                                               tCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAg                                                       <  1:1448683/150‑1 (MQ=255)
                                                                                               tCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAg                                                       <  1:136935/150‑1 (MQ=255)
                                                                                                  aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGGTTGAAGTAAAGGTGAGCCAGGTTGAACCCCTTCCCGCACAGGTg                                                    >  1:316557/1‑150 (MQ=255)
                                                                                                  aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                    >  1:1016424/1‑150 (MQ=255)
                                                                                                  aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                    <  2:1769147/150‑1 (MQ=255)
                                                                                                       ctgctgGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACg                                               >  1:1067217/1‑150 (MQ=255)
                                                                                                         ggtgGCGCAGGTGTTTAAGGACTAATGACCGCGGCGGCGGTATTTTCCGCCGCGGGGCTGATTTTTGATGGAGTACAGCTATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCGTTACCGAACAGGTGAAACGCt                                             <  1:946861/148‑1 (MQ=255)
                                                                                                            ggCGCAGGTGTTTAAGGACTAATGACATCGGCTGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCa                                          <  1:1651459/150‑1 (MQ=255)
                                                                                                            ggCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCa                                          >  1:847566/1‑150 (MQ=255)
                                                                                                              cgcAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACg                                        >  2:650957/1‑150 (MQ=255)
                                                                                                               gcAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGc                                       <  2:1356319/150‑1 (MQ=255)
                                                                                                                  ggTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtgg                                    <  2:1787418/150‑1 (MQ=255)
                                                                                                                    tgtTTAATGACTAATGACATCGGCGGAGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGAACAGCAATGTCAGACTATCAATTGTTTGAAGTACAGGTGAGCCAGGCTGAACCCCTTACCGAACAGGTGATACGCTTCACGCtggtg                                  <  2:1528059/150‑1 (MQ=255)
                                                                                                                    tgtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtg                                  <  2:1005371/150‑1 (MQ=255)
                                                                                                                     gtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtgg                                 >  2:827176/1‑150 (MQ=255)
                                                                                                                      tttAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGc                                <  1:1849664/150‑1 (MQ=255)
                                                                                                                        tAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGTAACGCTTCACGCTGGTGGCaa                              >  1:1263898/1‑150 (MQ=255)
                                                                                                                        tAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCaa                              >  1:590418/1‑150 (MQ=255)
                                                                                                                              cTAATGACATCCGCCGCGGTATTATCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAACTGTTTGAAGTACAGGGGAGCCAGGTTGAACCCCTTACCGAACCGGTGAAACGCTTCACGCTGGTGGCAACCGATg                        <  1:512302/150‑1 (MQ=255)
                                                                                                                                aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                      >  2:1073376/1‑150 (MQ=255)
                                                                                                                                   gACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCaaa                   >  2:1317402/1‑150 (MQ=255)
                                                                                                                                    aCATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCTGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGGTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAAc                  <  2:271585/150‑1 (MQ=255)
                                                                                                                                     gcccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAAcc                 >  1:434680/6‑150 (MQ=255)
                                                                                                                                      cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                >  1:1784486/5‑150 (MQ=255)
                                                                                                                                      cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGGTGAACCCCTTCCCGAACAGGTGAACCGCTTCACGCTGGTGGCAACCGATGGCAAACCa                >  1:295442/5‑150 (MQ=255)
                                                                                                                                      cccagcggcggAAAATACCGCCGCTGGGCTGAGTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                >  2:230830/5‑150 (MQ=255)
                                                                                                                                      aTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGTAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTAAAGGTGAGCAAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTCGTGGCAACCGATGGCAAACCa                >  2:76996/1‑150 (MQ=255)
                                                                                                                                      aTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                >  1:992208/1‑150 (MQ=255)
                                                                                                                                        cggcggcggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCAtt              >  1:330406/1‑150 (MQ=255)
                                                                                                                                         ggcggaggGATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACCGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCGTCACGCTGGTGGCAACCGATGGCAAACCATTa             <  1:726496/150‑1 (MQ=255)
                                                                                                                                             gcggTATTTTCCGCCGCTGGGCTGATTTTTGATTGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACTCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTg         >  2:654814/1‑150 (MQ=255)
                                                                                                                                             gcggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTg         <  1:487074/150‑1 (MQ=255)
                                                                                                                                              cggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAGCCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGc        >  2:370726/1‑150 (MQ=255)
                                                                                                                                                 tATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGtt     <  2:629274/150‑1 (MQ=255)
                                                                                                                                                  aTTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGGTTGAAGTACAGGTGAGCCTGGTGGAACCGCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAAGCGATGGCAAACCATTACCTGCGttt    <  2:1686829/150‑1 (MQ=255)
                                                                                                                                                    tttCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTAc  >  1:294366/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTAC  >  NC_000913/1885660‑1885957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: