Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,810 T→A 5.7% intergenic (+21/‑35) yeaW → / → yeaX putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,8100TA 5.7% 300.3 / 4.7 102intergenic (+21/‑35)yeaW/yeaXputative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base T (55/41);  new base A (4/2);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCG  >  NC_000913/1885663‑1885959
                                                                                                                                                   |                                                                                                                                                     
gAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCAGTTCCGAACATGGTATCGCCCAGTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTTATGACATCTGCGGCGGTATTTTcc                                                                                                                                                     >  2:726496/1‑150 (MQ=255)
      gCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGAAAATACcgccgcc                                                                                                                                               <  2:661928/150‑2 (MQ=255)
       cTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTg                                                                                                                                              <  2:1844825/150‑1 (MQ=255)
         gacaTCGCGGGGCTATCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTggg                                                                                                                                            <  1:10600/147‑1 (MQ=255)
         gAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTggg                                                                                                                                            >  1:1788602/1‑150 (MQ=255)
          aaaTCGCGTGGCTAGCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGc                                                                                                                                           <  1:450325/150‑1 (MQ=255)
           aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTTTCGCCCATTTCCATTATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGcg                                                                                                                                          >  2:851518/1‑149 (MQ=255)
           aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCt                                                                                                                                          >  1:402539/1‑150 (MQ=255)
           aaTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCt                                                                                                                                          >  2:1382740/1‑150 (MQ=255)
              cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCTTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGTGCTGAt                                                                                                                                       >  2:952401/1‑150 (MQ=255)
              cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAt                                                                                                                                       <  1:507897/150‑1 (MQ=255)
                cgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAttt                                                                                                                                     >  1:1761589/1‑150 (MQ=255)
                 gTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAtttt                                                                                                                                    >  1:164802/1‑150 (MQ=255)
                    gCTATCGGGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGa                                                                                                                                 <  1:1484374/150‑1 (MQ=255)
                     cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                                >  1:848294/1‑150 (MQ=255)
                     cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                                >  2:487074/1‑150 (MQ=255)
                      tATCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATg                                                                                                                               <  1:844768/150‑1 (MQ=255)
                          gTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGt                                                                                                                           >  1:625451/1‑150 (MQ=255)
                          gTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGt                                                                                                                           >  2:852953/1‑150 (MQ=255)
                               cAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGc                                                                                                                      <  1:1701951/150‑1 (MQ=255)
                                 ggggCGCATCATGGACGACAGTAGCTGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCaa                                                                                                                    >  1:271585/1‑150 (MQ=255)
                                  gggTGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAAt                                                                                                                   <  2:687611/150‑1 (MQ=255)
                                   ggCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGCACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATg                                                                                                                  >  2:1192878/1‑150 (MQ=255)
                                        atcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGa                                                                                                             >  1:1401298/1‑150 (MQ=255)
                                           atGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTa                                                                                                          >  1:9949/1‑150 (MQ=255)
                                           atGGCCGACAGTAGCGGTAGTGACATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTa                                                                                                          >  1:1019630/1‑150 (MQ=255)
                                             ggCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATc                                                                                                        <  1:251797/150‑1 (MQ=255)
                                               ccGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCaa                                                                                                      >  1:1437919/1‑150 (MQ=255)
                                                 gACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGAGCAGGTGTATAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTAATTTTTGATGGAGAACAGCAATGTCAGACTATCAAAt                                                                                                    >  1:1642153/1‑150 (MQ=255)
                                                  aCAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATATCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTAATGGAGTACAGCAAAGTAAGACTATCAAATg                                                                                                   >  1:1117299/1‑150 (MQ=255)
                                                      tAGCTGTAGTGGCATTTCCGAACATGGTATAGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTg                                                                                               <  2:86498/150‑1 (MQ=255)
                                                       aGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                              >  1:479274/1‑150 (MQ=255)
                                                       aGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                              >  1:472369/1‑150 (MQ=255)
                                                        tcGGTAGTGTCATTTCCGAACATGGTATCGCCCAATTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAAAGACATCGGCGGCGGTCTTTTCCGCCGCGGGGCTGATTGTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                             <  2:329544/149‑1 (MQ=255)
                                                        gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                             <  2:773881/150‑1 (MQ=255)
                                                         cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                            <  1:1858580/150‑1 (MQ=255)
                                                         cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                            <  2:472369/150‑1 (MQ=255)
                                                         cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                            <  2:479274/150‑1 (MQ=255)
                                                          ggTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAAGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCACACTATCAAAAGTTAGAAGt                                                                                           >  2:1862384/1‑150 (MQ=255)
                                                               tGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAgg                                                                                      >  2:592866/1‑150 (MQ=255)
                                                                ggCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGt                                                                                     >  1:1720378/1‑150 (MQ=255)
                                                                   aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                                  >  1:595920/1‑150 (MQ=255)
                                                                   aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTAACAAATGTTTGAAGTACAGGTGat                                                                                  >  2:389997/1‑149 (MQ=255)
                                                                   aTTTCCGAACATGGTATCGCAAATTTCCATAATCTGCTGGCGCAGGTGTTAAGGACTAATGCCATCGGAGGCTGTAATATCCGCCGCTGTGCTGATTTTTGATGGAGTACATCAATGTCAGCATATCAAATGTTTGAAGTACAGGTGAGc                                                                                 >  2:1283130/1‑150 (MQ=255)
                                                                      tCCGAACATCGTATCGCCCATTTCCATAATCTGCTGGCGCAGGAGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCa                                                                               <  2:1645471/150‑1 (MQ=255)
                                                                       ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                              >  2:1694089/1‑150 (MQ=255)
                                                                       ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                              >  2:1378648/1‑150 (MQ=255)
                                                                       ccGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAg                                                                              >  2:60417/1‑150 (MQ=255)
                                                                         taaCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGTTATATACCGCCGCTGGGCTGATTTTTGATGGAGAACACCAATCTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGt                                                                            <  1:103866/149‑1 (MQ=255)
                                                                         gAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACACCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAggg                                                                            >  2:1513606/1‑149 (MQ=255)
                                                                         gAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGGGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACATGTGAGCCAGGt                                                                            <  1:1759235/150‑1 (MQ=255)
                                                                          aaCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGtt                                                                           <  1:539319/150‑1 (MQ=255)
                                                                            cATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACGAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACACCAATGTCAGACTAACAAAAGTTTGAAGTAAAGGTGACCCAGGTTGa                                                                         >  1:667071/1‑150 (MQ=255)
                                                                                  aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                                   >  1:219750/1‑150 (MQ=255)
                                                                                   tCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCtt                                                                  <  1:515167/150‑1 (MQ=255)
                                                                                   tCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCtt                                                                  >  1:1325988/1‑150 (MQ=255)
                                                                                    cGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTa                                                                 >  1:226071/1‑150 (MQ=255)
                                                                                     gCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                                <  1:1244000/150‑1 (MQ=255)
                                                                                     gCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                                >  2:1837583/1‑150 (MQ=255)
                                                                                       ccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCg                                                              <  2:1373916/150‑1 (MQ=255)
                                                                                          ataccATAATCTGCGCGCGCAGGTGTTTAAGGACTAATCACCTCGGCGGCGGTATTTTCCGCCGCGGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAAAGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAAc                                                           <  2:1788602/147‑1 (MQ=255)
                                                                                            tCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAg                                                         <  1:136935/150‑1 (MQ=255)
                                                                                            tCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAg                                                         <  1:1448683/150‑1 (MQ=255)
                                                                                               aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGGTTGAAGTAAAGGTGAGCCAGGTTGAACCCCTTCCCGCACAGGTg                                                      >  1:316557/1‑150 (MQ=255)
                                                                                               aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                      <  2:1769147/150‑1 (MQ=255)
                                                                                               aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                      >  1:1016424/1‑150 (MQ=255)
                                                                                                    ctgctgGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACg                                                 >  1:1067217/1‑150 (MQ=255)
                                                                                                      ggtgGCGCAGGTGTTTAAGGACTAATGACCGCGGCGGCGGTATTTTCCGCCGCGGGGCTGATTTTTGATGGAGTACAGCTATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCGTTACCGAACAGGTGAAACGCt                                               <  1:946861/148‑1 (MQ=255)
                                                                                                         ggCGCAGGTGTTTAAGGACTAATGACATCGGCTGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCa                                            <  1:1651459/150‑1 (MQ=255)
                                                                                                         ggCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCa                                            >  1:847566/1‑150 (MQ=255)
                                                                                                           cgcAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACg                                          >  2:650957/1‑150 (MQ=255)
                                                                                                            gcAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGc                                         <  2:1356319/150‑1 (MQ=255)
                                                                                                               ggTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtgg                                      <  2:1787418/150‑1 (MQ=255)
                                                                                                                 tgtTTAATGACTAATGACATCGGCGGAGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGAACAGCAATGTCAGACTATCAATTGTTTGAAGTACAGGTGAGCCAGGCTGAACCCCTTACCGAACAGGTGATACGCTTCACGCtggtg                                    <  2:1528059/150‑1 (MQ=255)
                                                                                                                 tgtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtg                                    <  2:1005371/150‑1 (MQ=255)
                                                                                                                  gtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtgg                                   >  2:827176/1‑150 (MQ=255)
                                                                                                                   tttAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGc                                  <  1:1849664/150‑1 (MQ=255)
                                                                                                                     tAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGTAACGCTTCACGCTGGTGGCaa                                >  1:1263898/1‑150 (MQ=255)
                                                                                                                     tAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCaa                                >  1:590418/1‑150 (MQ=255)
                                                                                                                       aGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAAcc                              >  2:600536/1‑150 (MQ=255)
                                                                                                                           cTAATGACATCCGCCGCGGTATTATCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAACTGTTTGAAGTACAGGGGAGCCAGGTTGAACCCCTTACCGAACCGGTGAAACGCTTCACGCTGGTGGCAACCGATg                          <  1:512302/150‑1 (MQ=255)
                                                                                                                             aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                        >  2:1073376/1‑150 (MQ=255)
                                                                                                                                gACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCaaa                     >  2:1317402/1‑150 (MQ=255)
                                                                                                                                 aCATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCTGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGGTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAAc                    <  2:271585/150‑1 (MQ=255)
                                                                                                                                   cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                  >  1:1784486/5‑150 (MQ=255)
                                                                                                                                   cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGGTGAACCCCTTCCCGAACAGGTGAACCGCTTCACGCTGGTGGCAACCGATGGCAAACCa                  >  1:295442/5‑150 (MQ=255)
                                                                                                                                   cccagcggcggAAAATACCGCCGCTGGGCTGAGTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                  >  2:230830/5‑150 (MQ=255)
                                                                                                                                   aTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGTAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTAAAGGTGAGCAAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTCGTGGCAACCGATGGCAAACCa                  >  2:76996/1‑150 (MQ=255)
                                                                                                                                   aTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                  >  1:992208/1‑150 (MQ=255)
                                                                                                                                     cggcggcggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCAtt                >  1:330406/1‑150 (MQ=255)
                                                                                                                                      ggcggaggGATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACCGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCGTCACGCTGGTGGCAACCGATGGCAAACCATTa               <  1:726496/150‑1 (MQ=255)
                                                                                                                                          gcggTATTTTCCGCCGCTGGGCTGATTTTTGATTGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACTCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTg           >  2:654814/1‑150 (MQ=255)
                                                                                                                                          gcggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTg           <  1:487074/150‑1 (MQ=255)
                                                                                                                                           cggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAGCCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGc          >  2:370726/1‑150 (MQ=255)
                                                                                                                                              tATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGtt       <  2:629274/150‑1 (MQ=255)
                                                                                                                                               aTTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGGTTGAAGTACAGGTGAGCCTGGTGGAACCGCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAAGCGATGGCAAACCATTACCTGCGttt      <  2:1686829/150‑1 (MQ=255)
                                                                                                                                                 tttCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTAc    >  1:294366/1‑150 (MQ=255)
                                                                                                                                                   tCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGGAAACCATTACCTGCGTTTACCg  >  2:286617/1‑150 (MQ=255)
                                                                                                                                                   tCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCg  >  2:1741997/1‑150 (MQ=255)
                                                                                                                                                   tCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTGGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACATGCGTATACCg  >  2:236887/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCG  >  NC_000913/1885663‑1885959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: