Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,915 A→C 5.8% intergenic (‑169/+36) fadD ← / ← yeaY acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,9150AC 5.8% 391.2 / 7.0 138intergenic (‑169/+36)fadD/yeaYacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base A (64/64);  new base C (8/2);  total (72/66)
Fisher's exact test for biased strand distribution p-value = 9.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GTGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTG  >  NC_000913/1889766‑1890061
                                                                                                                                                     |                                                                                                                                                  
gtgtTCGTCTTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGa                                                                                                                                                    <  1:942664/150‑1 (MQ=255)
  gtTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGAcc                                                                                                                                                  >  2:1044968/1‑150 (MQ=255)
        cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCCACGATAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                            >  1:365444/1‑150 (MQ=255)
        cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                            >  1:963359/1‑150 (MQ=255)
            aTCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtt                                                                                                                                        >  1:1747061/1‑150 (MQ=255)
             tCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGttt                                                                                                                                       >  2:805605/1‑150 (MQ=255)
             tCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAAAAAGAAACAGCGGCTGGTCCGCTGtttc                                                                                                                                      <  1:256580/150‑1 (MQ=255)
              cGCAACCCCAAATGATATCCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCCCTATTTATTTTTATCTATACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtgtc                                                                                                                                      >  1:157627/1‑150 (MQ=255)
              cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTAGTTTTATCTTTCCCGTAAGAATGCAGAAACAGCGGCCCCGCCGCGGtttc                                                                                                                                      >  2:979409/1‑150 (MQ=255)
               gCAACCCAAAATGATATACATGCCGATAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttct                                                                                                                                     <  2:612826/150‑1 (MQ=255)
                cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctt                                                                                                                                    >  1:861338/1‑150 (MQ=255)
                   ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAATTTAGCGAAGCGCTTCAATATTTATTTTTATCTTTACCTTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                 >  1:505637/1‑150 (MQ=255)
                   ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                 >  2:1792653/1‑150 (MQ=255)
                   ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                 >  1:737627/1‑150 (MQ=255)
                    cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                <  1:821326/150‑1 (MQ=255)
                    cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACAATCTTGATAATTACCCAACGAAAAGGTTGCTAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCAAGCCGCTGtttctttttg                                                                                                                                >  2:1419149/1‑149 (MQ=255)
                    cacAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctgcatt                                                                                                                                <  1:1183346/148‑6 (MQ=255)
                     ccAATTGATATACATGCCTTTAACATAATATTATCTCATCATACCAGCTTGATAATTACCCAACTAAAAGGTTGCGAAGCGCGTCTCTATTTATTTTTATCTTTACAGTATGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttgt                                                                                                                               <  1:436747/150‑3 (MQ=255)
                     ccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAACAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttct                                                                                                                               <  1:1114990/150‑1 (MQ=255)
                      cAAATGATATACATGCCGTTAACATAATATTAACTCAACATACCAGCTTGATAATTACCCAACGAAAAGGTAGCGAAGCGCGTCCCTAATTATTTTTATATTTACCGTAAGAAAGCAGAAACAGCGGACCAGCCGCAGtttctttttctt                                                                                                                              >  1:532912/1‑150 (MQ=255)
                       aaaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTttctttttcttt                                                                                                                             >  2:927757/1‑150 (MQ=255)
                              ataCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCGTTTTCTTTAAAAACa                                                                                                                      >  2:191097/1‑150 (MQ=255)
                              ataCATCCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCATCCGCTGTTTCTTTTTCTTTAAAAACa                                                                                                                      <  1:1102640/150‑1 (MQ=255)
                               taCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACaa                                                                                                                     >  1:1470320/1‑150 (MQ=255)
                                aCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAg                                                                                                                    <  1:530870/150‑1 (MQ=255)
                                aCATGCCGTTAACATAATATTAACTCATCATACCAGCATGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAg                                                                                                                    <  1:1522176/150‑1 (MQ=255)
                                 cATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGTAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGc                                                                                                                   <  1:1557869/150‑1 (MQ=255)
                                 cATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTATTTAAAAACAAGc                                                                                                                   >  2:1564252/1‑150 (MQ=255)
                                  aTGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCa                                                                                                                  >  1:539267/1‑150 (MQ=255)
                                    gCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAATGATGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAat                                                                                                                <  1:1233325/150‑1 (MQ=255)
                                    gCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAat                                                                                                                <  2:737627/150‑1 (MQ=255)
                                    gCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAatt                                                                                                               <  2:198854/150‑1 (MQ=255)
                                     acGTTAACATATTATTAACTCATCATACCAGCTTGATAATTACCCAACGCATAGGTTGAGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAatt                                                                                                               <  2:762119/149‑1 (MQ=255)
                                               aataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTAc                                                                                                     <  2:1752186/150‑1 (MQ=255)
                                                  atTAACTCATAATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAAc                                                                                                  <  1:1237133/150‑1 (MQ=255)
                                                   ttAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACAATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTAATTTACAACt                                                                                                 >  2:893954/1‑150 (MQ=255)
                                                      aCTCATCATACCAGCTTGATTATTACACAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCTTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTAATCAGTTACAACTGtt                                                                                              <  1:254965/150‑1 (MQ=255)
                                                      aCTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                              <  2:362046/150‑1 (MQ=255)
                                                         catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGt                                                                                           <  2:1070415/150‑1 (MQ=255)
                                                         catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGt                                                                                           >  2:1344836/1‑150 (MQ=255)
                                                         catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTAATCAGTTACAACTGTTTGt                                                                                           >  1:670609/1‑150 (MQ=255)
                                                             atACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAAATGTTTGTActc                                                                                       >  2:1645812/1‑150 (MQ=255)
                                                              tACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTActct                                                                                      >  2:529974/1‑150 (MQ=255)
                                                                ccAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcg                                                                                    >  2:1480490/1‑150 (MQ=255)
                                                                ccAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcg                                                                                    >  1:1853350/1‑150 (MQ=255)
                                                                   gCTTGATAATTACCCAACGAAATGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCgg                                                                                 <  1:906619/150‑1 (MQ=255)
                                                                   gCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCgg                                                                                 >  2:1543354/1‑150 (MQ=255)
                                                                      tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                              >  1:74127/1‑150 (MQ=255)
                                                                      tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                              >  1:369842/1‑150 (MQ=255)
                                                                      tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                              <  2:507264/150‑1 (MQ=255)
                                                                      tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                              <  1:903754/150‑1 (MQ=255)
                                                                       gATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCTCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGcc                                                                             >  1:571756/1‑150 (MQ=255)
                                                                       gATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTCTTTCTTTTTCTTTATAAACAAGCAATTATTCAGTTACATCTGTTTGTACTCTCGCGCGGcc                                                                             >  2:304638/1‑150 (MQ=255)
                                                                         tAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCgg                                                                           >  1:1421211/1‑150 (MQ=255)
                                                                          aaTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                          <  1:1850263/150‑1 (MQ=255)
                                                                           aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                         <  1:1044968/150‑1 (MQ=255)
                                                                           aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                         >  2:854898/1‑150 (MQ=255)
                                                                           aTTACCCAACGAAAAGGTAGCGAAGCGCGTCACTAATTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCgggc                                                                         <  1:427708/150‑2 (MQ=255)
                                                                            ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                        <  1:805605/150‑1 (MQ=255)
                                                                            ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                        <  2:861338/150‑1 (MQ=255)
                                                                              aCCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATta                                                                      <  1:1698273/150‑1 (MQ=255)
                                                                               cccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtat                                                                     <  1:529974/150‑1 (MQ=255)
                                                                                ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGTACCAGCCGCTGTTTCATTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGACGGGATtata                                                                    >  1:1155090/1‑150 (MQ=255)
                                                                                ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTGTTTTTCTTTAAACCCAAGCAATTATTCAGTTACAACTGTTTGGACTCGCGCGGGGCCGGGATgata                                                                    >  2:287761/1‑150 (MQ=255)
                                                                                ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATtata                                                                    >  2:1591680/1‑150 (MQ=255)
                                                                                ccAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGAAGAAACAGCGGACCAGCCGCTGTTTCTTATTCTTTAAAAACAAGCAATTATTCAGTAACAACTGTTTGTACTCTCGCGGGGCCGGGATtaaa                                                                    >  1:407726/1‑148 (MQ=255)
                                                                                 cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                   >  1:1089464/1‑150 (MQ=255)
                                                                                 cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTAATCATTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                   >  2:1679902/1‑150 (MQ=255)
                                                                                      aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCCGACCAGCCGCTGGTTGTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGAACTCAAGCTGGGCCGTGTTTTTACCAgac                                                              >  1:1151022/1‑148 (MQ=255)
                                                                                          ggTTGCGAAGCGCGTCACTATTTATTTTTATCTTAACCGTAAGAATGCAGAAACAGCGGACCAGCCCCTGTTTCATTTTCTATAAAAACAAGCAATTATTCAGTTACAACTTTTTGTACTCTCGCGGGGCCGGGATAATACAAGCCCCag                                                          >  2:1160555/1‑149 (MQ=255)
                                                                                           gTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCTCTGTTTCTTTTTCTTTAAAAACTAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCTGGTATAATCCCGGccccgcg                                                         <  2:250663/150‑4 (MQ=255)
                                                                                           gTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCTGGTATAATCCCGGccccgcg                                                         <  2:1229211/150‑4 (MQ=255)
                                                                                            ttGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATcc                                                        >  1:1203683/1‑150 (MQ=255)
                                                                                             tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccg                                                       >  1:1278276/1‑150 (MQ=255)
                                                                                               cGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgcc                                                     >  1:1010988/1‑150 (MQ=255)
                                                                                                     gcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGCAACAGCAGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGcc                                               <  2:121692/150‑1 (MQ=255)
                                                                                                         cACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                          >  2:1635467/1‑119 (MQ=255)
                                                                                                          aCTATTTATTTTTATCTTTACCGTAAGAATTCATAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTCCAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCGTCCGCCGTAGCCATAgg                                          <  2:505637/150‑1 (MQ=255)
                                                                                                          aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAgg                                          <  1:1569259/150‑1 (MQ=255)
                                                                                                             ttttatttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgcc                                       <  1:979409/149‑1 (MQ=255)
                                                                                                                 atttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcc                                   >  1:709671/1‑150 (MQ=255)
                                                                                                                    tttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTTAAAACAAGCAATTATTAAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCAcc                                >  1:874250/1‑149 (MQ=255)
                                                                                                                    tttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACg                                <  1:231602/150‑1 (MQ=255)
                                                                                                                          tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGcc                          <  2:539267/150‑1 (MQ=255)
                                                                                                                            tACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTGTTTTTCTTTAAAAGCAAGCAATTATTCAGTTACAACTGTTTGTTCTCTCGCGGGGCCGGGAGTATACCAGCCCCATCCGCCGTAGCCTTAGGGCCCGCCACGACCGCCAt                        >  1:393500/1‑150 (MQ=255)
                                                                                                                               cGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTGTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGAACACTCCCGGGGCCGGGATTATCCCAGCCCCCTCCGCCGTAGCCATAGGGCCCGCCCCGCCCGCCATaaa                     >  2:1131941/1‑150 (MQ=255)
                                                                                                                                   aagaaaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCa                 >  1:18670/8‑150 (MQ=255)
                                                                                                                                     gaaaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATg               >  1:206180/6‑150 (MQ=255)
                                                                                                                                     aaTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATg               >  2:717418/1‑150 (MQ=255)
                                                                                                                                      aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATgg              <  2:538086/150‑1 (MQ=255)
                                                                                                                                         cAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATc           >  1:435087/1‑150 (MQ=255)
                                                                                                                                          aGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCa          >  1:202868/1‑150 (MQ=255)
                                                                                                                                           gAAACAGCGGACCAGCCGCGGTTTCGTTTTCTTTAAAAACAAGCAATTATTCAGGTACAACGGTTTGTACTCTCGCGGGGCCGGGAGTATACCAGCCCCATCCGCCGTAGCCAGAGGGCCAGCCCCGACCGCCATAAAACCATGGCTCaa         >  1:686167/1‑150 (MQ=255)
                                                                                                                                               cAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCCCGACCGCCATAAAACCATGGATCAATCgg     >  2:1367892/1‑150 (MQ=255)
                                                                                                                                                aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGTTTATACCCGACACATCCGCCGTAGCCATAGGTCCAGCCACGACCGCAAAAAAACCATTGATCAATCgtc    >  1:1170029/1‑148 (MQ=255)
                                                                                                                                                aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc    <  1:1543354/150‑1 (MQ=255)
                                                                                                                                                 gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt   <  1:802117/150‑1 (MQ=255)
                                                                                                                                                 gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCt   >  2:964087/1‑150 (MQ=255)
                                                                                                                                                 gCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACAGTTTGTACTCTCGCGGGGCCGGGATTATACCAGACCCATCCGCCGTAGCCATAGGGCCATCCACGACCGCCATAAAACAATGGATCAATCGGCt   >  2:888709/1‑150 (MQ=255)
                                                                                                                                                  cGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTg  <  1:1116739/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTGTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTG  >  NC_000913/1889766‑1890061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: