Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,929,028 A→G 6.9% intergenic (‑189/+20) ptrB ← / ← yebE protease II/DUF533 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,929,0280AG 6.9% 259.8 / 7.4 87intergenic (‑189/+20)ptrB/yebEprotease II/DUF533 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/45);  new base G (2/4);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

AAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAAT  >  NC_000913/1928880‑1929176
                                                                                                                                                    |                                                                                                                                                    
aaaaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGACGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAg                                                                                                                                                     <  1:736222/150‑1 (MQ=255)
  aaCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGc                                                                                                                                                   <  1:587651/150‑1 (MQ=255)
      cACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATTGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATc                                                                                                                                               >  1:1122860/1‑150 (MQ=255)
       aCCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATcc                                                                                                                                              <  2:844475/150‑1 (MQ=39)
          ggggATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCaga                                                                                                                                           <  1:1054146/150‑1 (MQ=38)
           gggATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCagaa                                                                                                                                          <  1:1095820/150‑2 (MQ=38)
           gggATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCagaa                                                                                                                                          <  1:1606336/150‑2 (MQ=38)
             gATTCAAGCGACACACGGCAGTCATTGCTTAGATGATGTCTGGTAATGGCGCGGATATCGAATGTTATGCAGACAGTGAAAATCAGCCTGCTGAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCagagag                                                                                                                                        <  2:311754/150‑1 (MQ=255)
             gATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCagagag                                                                                                                                        >  1:1425888/1‑150 (MQ=255)
              aTGCAAGCGAAACAGGGGAGTCATTGCTTTGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGc                                                                                                                                       <  1:1158304/150‑1 (MQ=255)
                gCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCgg                                                                                                                                     >  2:1393463/1‑150 (MQ=255)
                 cAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGa                                                                                                                                    >  2:373820/1‑150 (MQ=255)
                       aaaCAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAattatt                                                                                                                              >  1:448248/1‑150 (MQ=255)
                       aaaCAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTCGATAGCCCGAAATAGTCAACTTCAGGCTATCCATAGAGCGGAattatt                                                                                                                              >  1:190524/1‑150 (MQ=255)
                           aGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGc                                                                                                                          <  1:1780979/150‑1 (MQ=255)
                                  tCATTGCTTAGATGATGACAGGTAATGGCGCGGATATAGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATATCCCGAAATAGTCAACTTCATGATATCCAGAGAGCGGAATTATTCCGCCAAAGTg                                                                                                                   <  2:1758261/150‑1 (MQ=255)
                                   cATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGc                                                                                                                  >  2:1871990/1‑150 (MQ=255)
                                       gCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGttt                                                                                                              >  1:380769/1‑150 (MQ=255)
                                          tAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTg                                                                                                           <  2:1854816/150‑1 (MQ=255)
                                          tAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTg                                                                                                           >  1:57841/1‑150 (MQ=255)
                                           tgatgatGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGc                                                                                                          <  1:787720/149‑1 (MQ=255)
                                           aGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGc                                                                                                          <  1:1745282/150‑1 (MQ=255)
                                           aGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGc                                                                                                          <  1:1427588/150‑1 (MQ=255)
                                                 tgaCAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCg                                                                                                    <  2:609116/150‑1 (MQ=255)
                                                     aGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGAt                                                                                                <  2:1431540/150‑1 (MQ=255)
                                                      ggTAATGGCGCGGATATCGAATGGTATGCAGACAGAGAAAATCAGCCTGTTCATATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATc                                                                                               <  2:1017786/150‑1 (MQ=255)
                                                       gTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATcg                                                                                              >  1:351359/1‑150 (MQ=255)
                                                           tGGCGCGTATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGtt                                                                                          <  2:851549/150‑1 (MQ=255)
                                                           tGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGtt                                                                                          <  1:1084607/150‑1 (MQ=255)
                                                             gcgcGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCa                                                                                        <  2:1498321/150‑1 (MQ=255)
                                                              cgcgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCaa                                                                                       <  1:1226405/150‑1 (MQ=255)
                                                                cgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATg                                                                                     >  2:1007151/1‑150 (MQ=255)
                                                                cgGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATg                                                                                     <  2:431566/150‑1 (MQ=255)
                                                                 ggATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGc                                                                                    <  2:1851427/150‑1 (MQ=255)
                                                                  gATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGcc                                                                                   >  1:584143/1‑150 (MQ=255)
                                                                   atatCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCg                                                                                  <  1:13571/150‑1 (MQ=255)
                                                                    tatCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGt                                                                                 >  1:1524206/1‑150 (MQ=255)
                                                                     atCGAATGTTATGCAGACAGAGAAAATCAGCCTGTGCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTc                                                                                <  2:231099/150‑1 (MQ=255)
                                                                       cGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCAc                                                                              <  2:928001/150‑1 (MQ=255)
                                                                        gAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACg                                                                             >  2:158936/1‑150 (MQ=255)
                                                                         aaTTTTATGCAGAAAGACAAAATCAGCCTGTTCAAATGTCTCTGCGATTCTGCATATCCAGAAATAGTCAACATCAGGCTATCCATATAGCGTAATTATTCAGCCAAAGTGCGTTTTTGCTTTTCGAGATCGCGTTCAATGCCGTCACGa                                                                            <  1:1711632/150‑1 (MQ=255)
                                                                            gTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACa                                                                         <  1:825292/150‑1 (MQ=255)
                                                                                    gacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgggg                                                                 <  2:1259310/150‑1 (MQ=255)
                                                                                    gacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgggg                                                                 <  1:637549/150‑1 (MQ=255)
                                                                                    gacagaGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTgggg                                                                 <  2:23058/150‑1 (MQ=255)
                                                                                       agagAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGAAAGCCCGAAATAGGCAACTTCAGGCTATCCAGAGAGCGGAATTTTTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAt                                                              <  2:1680674/150‑1 (MQ=255)
                                                                                        gagaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                             >  1:369334/1‑150 (MQ=255)
                                                                                        gagaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCAGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtt                                                             >  1:176940/1‑150 (MQ=255)
                                                                                          gaAAATCAGCCTGTTCAAATGGCTGTGCGATTCTTGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCACAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGAtttt                                                           <  1:144205/150‑1 (MQ=255)
                                                                                           aaaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTc                                                          >  1:898769/1‑150 (MQ=255)
                                                                                            aaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCa                                                         >  1:1339025/1‑150 (MQ=255)
                                                                                            aaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCa                                                         >  2:1306128/1‑150 (MQ=255)
                                                                                            aaaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGATTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCa                                                         <  1:243481/150‑1 (MQ=255)
                                                                                             aaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAg                                                        <  2:1616718/150‑1 (MQ=255)
                                                                                             aaTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGGCAACTTCAGGCTATCCAGAGAGCGGAATTATGCCGCCAAAGTGCGGTTTTTCTGTTCGAGATCGCGTTCAATGCCGCCACGAACATCCTGGGGGATTTTCAg                                                        <  2:353209/150‑1 (MQ=255)
                                                                                               tCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAgcg                                                      >  1:1583607/1‑150 (MQ=255)
                                                                                                      gTTAAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCAcc                                               >  1:1249704/1‑150 (MQ=255)
                                                                                                          aaaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGt                                           <  2:448248/150‑1 (MQ=255)
                                                                                                           aaTGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTg                                          <  1:1005105/150‑1 (MQ=255)
                                                                                                                cTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTc                                     <  2:1101799/150‑1 (MQ=255)
                                                                                                                   tgCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAgg                                  <  1:1301722/150‑1 (MQ=255)
                                                                                                                    gCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGt                                 >  2:1290078/1‑150 (MQ=255)
                                                                                                                     cGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTa                                >  1:547828/1‑150 (MQ=255)
                                                                                                                        ttCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACt                             >  2:801436/1‑150 (MQ=255)
                                                                                                                          cTGGATAGCCTGAAGTTGACTATTTCGGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGc                           >  1:1609352/1‑150 (MQ=38)
                                                                                                                            ggATAGCCTGAAGTTGACTATTTCGGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCTCGTCACCCAGTGCATTCAGGTAACTGCGt                         >  1:265876/1‑150 (MQ=38)
                                                                                                                             gATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGtt                        >  1:1162627/1‑150 (MQ=255)
                                                                                                                               tAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTcc                      <  1:1703312/150‑1 (MQ=255)
                                                                                                                               tAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTcc                      >  2:7358/1‑150 (MQ=255)
                                                                                                                                 gCCCGAAATAGTCAACTTCAGGCTATCCAGCTAGCGGAATTATTCCGCCAAAAAGCGTTTTTGCTTTACGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCTCGTCACCCAGTGAATTCAGGTAACTGCGTTCCAt                    >  2:1525823/1‑150 (MQ=255)
                                                                                                                                   ccGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACCTCCTGGGGGAGTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATaa                  >  1:731879/1‑150 (MQ=255)
                                                                                                                                      aaaaatgcAACATCCGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAt               <  1:362104/143‑1 (MQ=255)
                                                                                                                                       aaTAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATg              <  1:1814684/150‑1 (MQ=255)
                                                                                                                                          aGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtc           >  2:1567385/1‑150 (MQ=255)
                                                                                                                                           gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca          <  2:1388692/150‑1 (MQ=255)
                                                                                                                                           gTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtca          <  2:872691/150‑1 (MQ=255)
                                                                                                                                             cAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaat        <  1:158936/150‑1 (MQ=255)
                                                                                                                                             cAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaat        >  1:1515192/1‑150 (MQ=255)
                                                                                                                                             cAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGGCACGAACATCCTGGGGCATTTTCAGCGCGTCACCCAGGGCATTCAGGTTCATGCGTTCCATAAAGGGGgcaat        >  2:336891/1‑150 (MQ=255)
                                                                                                                                             cAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTGCGAGATCGCGTTCAATGCCGTCACCAACATCCTGGGGGATTTTCAGCTCGTCACCCAGTGCATTCAGGTAACTGCGATCCATATAATGGgcaat        >  2:268647/1‑150 (MQ=255)
                                                                                                                                               aCTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGTGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatat      <  1:1118875/150‑1 (MQ=255)
                                                                                                                                               aCTTAAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGACGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGATCCATAAAATGGtcaatat      >  1:601324/1‑150 (MQ=255)
                                                                                                                                                cTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatatc     <  2:971482/150‑1 (MQ=255)
                                                                                                                                                cTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatatc     <  2:1111121/150‑1 (MQ=255)
                                                                                                                                                cTTCAGGCTATCCAGAGAGCGGAATTATACCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatatc     <  2:317824/150‑1 (MQ=255)
                                                                                                                                                   cAGGCTATCCAGCGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatatcaat  >  2:155036/1‑150 (MQ=255)
                                                                                                                                                   cAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGtcaatatcaat  >  2:1063469/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAAT  >  NC_000913/1928880‑1929176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: