Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 146,293 A→C 5.4% N405H (AAC→CAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913146,2930AC 5.4% 386.3 / 10.5 131N405H (AAC→CAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (66/58);  new base C (4/3);  total (70/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.75e-01

Only 100 of 133 total aligned reads displayed.

ACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGAC  >  NC_000913/146144‑146442
                                                                                                                                                     |                                                                                                                                                     
aCGCCGTGAAGGCAGCAAACGATGCCGGATTTCCCCTGGCGGTGACAACCATGAAAGGCAAAGTAAACCCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGCTTCGCTGGAGACGATGTCGCGGCTGGTGAGTa                                                                                                                                                       >  1:125016/1‑150 (MQ=255)
   ccGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAAcc                                                                                                                                                    >  1:943057/1‑150 (MQ=255)
    cGTGAGGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCa                                                                                                                                                   >  1:1313897/1‑150 (MQ=255)
    cGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCa                                                                                                                                                   >  1:1509914/1‑150 (MQ=255)
      tGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGc                                                                                                                                                 <  2:798534/150‑1 (MQ=255)
        aaGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACCACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGCCTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGGGAGTAACCAGCCg                                                                                                                                               >  1:113514/1‑150 (MQ=255)
         aGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTTACTCACCAGCCGc                                                                                                                                              <  1:746455/150‑1 (MQ=255)
          ggCAGCAAACGATGCCTGAGTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGCGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTTACTCACCAGCCGcg                                                                                                                                             <  2:171438/150‑2 (MQ=255)
          ggCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCa                                                                                                                                             <  1:718678/150‑1 (MQ=255)
           gcagcaAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAg                                                                                                                                            <  1:799027/150‑1 (MQ=255)
           gcagcaAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAg                                                                                                                                            <  1:475200/150‑1 (MQ=255)
            cagcaAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAgg                                                                                                                                           >  2:1324509/1‑150 (MQ=255)
            cagcaAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAgg                                                                                                                                           >  2:515790/1‑150 (MQ=255)
               caAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTATGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGAt                                                                                                                                        >  2:563594/1‑150 (MQ=255)
               caAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGAt                                                                                                                                        <  2:1384887/150‑1 (MQ=255)
                aaaCGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATa                                                                                                                                       >  1:1722196/1‑150 (MQ=255)
                aaaCGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAACTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTCAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATa                                                                                                                                       <  2:163289/150‑1 (MQ=255)
                 aaCGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGACCGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGAtta                                                                                                                                      >  1:66966/1‑148 (MQ=255)
                      tGCCGGATTTCACCTGGCGGTGACAACCATGACAGGCAAAGTAAAACCGGGGGTTAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATc                                                                                                                                 <  2:126986/150‑1 (MQ=255)
                       gCCGGTTTTCCACTTGCGGTGAAAACCATTAAACGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGATGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCa                                                                                                                                <  2:1183589/150‑1 (MQ=255)
                       gCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCa                                                                                                                                <  2:755320/150‑1 (MQ=255)
                       cccGGATTTCACCTCGCGGAGACAACCAAGATAGCCAAAGTAAAACCGGGGGATAATCCGTTGTTACAAAAACGACTTTATATCTAAAGAACGGATTCGCTGGAGACGAACTCGCGGCTGGTGAGAAACCAGCCGCAGGGATAACAATCa                                                                                                                                <  1:108561/149‑1 (MQ=255)
                        ccGGATTTCACCTGGCGGTGACGACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCaa                                                                                                                               >  1:1204908/1‑150 (MQ=255)
                        ccGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCaa                                                                                                                               >  1:1758417/1‑150 (MQ=255)
                         cGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAg                                                                                                                              <  2:709098/150‑1 (MQ=255)
                         cGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAg                                                                                                                              >  2:1556870/1‑150 (MQ=255)
                          gtttttCACCTGGCGGTGACAACCATGAATGTCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGTATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACATTCATGc                                                                                                                             <  1:643304/147‑1 (MQ=255)
                               tCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGCTTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCt                                                                                                                        >  1:882388/1‑150 (MQ=255)
                                cACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTg                                                                                                                       <  2:352870/150‑1 (MQ=255)
                                  ccTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTa                                                                                                                     >  2:1239364/1‑150 (MQ=255)
                                    tGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATATCAATCAAGCAACCTGTAcc                                                                                                                   >  2:415363/1‑150 (MQ=255)
                                    tGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTAcc                                                                                                                   >  2:1009039/1‑150 (MQ=255)
                                        ggTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGaa                                                                                                               >  1:1279178/1‑150 (MQ=255)
                                           gACAACCATGAAAGGCAAAGTAAAACCGGGGTATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCg                                                                                                            <  2:959442/150‑1 (MQ=255)
                                           gACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTGGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCg                                                                                                            <  2:1396808/150‑1 (MQ=255)
                                             cAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCt                                                                                                          >  1:1325559/1‑150 (MQ=255)
                                             cAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCt                                                                                                          >  1:503539/1‑150 (MQ=255)
                                               aCCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCttt                                                                                                        >  1:724015/1‑150 (MQ=255)
                                                  aTGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGACTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTcgc                                                                                                     <  2:1841172/150‑1 (MQ=255)
                                                       aGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTAc                                                                                                <  1:259198/150‑1 (MQ=255)
                                                         gCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGt                                                                                              >  1:1264939/1‑150 (MQ=255)
                                                         gCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGt                                                                                              >  2:692221/1‑150 (MQ=255)
                                                           aaaGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGttt                                                                                            <  1:1854195/150‑1 (MQ=255)
                                                              gTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcat                                                                                         <  1:538393/150‑1 (MQ=255)
                                                                aaaaCCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGTCGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGtttcattt                                                                                       <  2:1747827/150‑1 (MQ=255)
                                                                aaaaCCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGAAACCTGTACCGGAATCGCTTTCGCGGGACGtttcattt                                                                                       >  2:1813911/1‑150 (MQ=255)
                                                                    ccGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTtcatttcatt                                                                                   <  1:92031/150‑1 (MQ=255)
                                                                     ccggggATAAACCGTTTGTAATAAAAAGTATTTATATCTTAAGAACGGCTTCGCTGGTGAACTTTTCGCGGCTGGGTCTTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTg                                                                                  <  2:537441/148‑1 (MQ=255)
                                                                     cGGGGGATTATCCGTTGTTTCTAAAACGCCCTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTg                                                                                  <  1:270325/150‑1 (MQ=255)
                                                                        gggATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCg                                                                               <  1:1333494/150‑1 (MQ=255)
                                                                         ggATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGc                                                                              <  1:165685/150‑1 (MQ=255)
                                                                            tAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCtt                                                                           <  1:383130/150‑1 (MQ=255)
                                                                               tCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCaa                                                                        <  2:388302/150‑1 (MQ=255)
                                                                                     gttACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAgg                                                                  >  1:35787/1‑150 (MQ=255)
                                                                                      ttACTAAAACTAATTTTTATCTTAAGTAAGGATTAGCTGTAGAAGATGTCGCGTCTGGTGAGTAACCATCCGCATGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGc                                                                 <  1:1174097/150‑1 (MQ=255)
                                                                                       tACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCACCCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCg                                                                >  2:1102899/1‑150 (MQ=255)
                                                                                        aCTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGa                                                               >  1:483677/1‑150 (MQ=255)
                                                                                        aCTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCAGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTTTGGCCATCAAAATAGGCGa                                                               >  2:1370601/1‑150 (MQ=255)
                                                                                               cGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTggg                                                        >  2:1506413/1‑150 (MQ=255)
                                                                                               cGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTggg                                                        <  2:98138/150‑1 (MQ=255)
                                                                                               cGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTACAAAATAGGCGACGTTggg                                                        <  2:1048566/150‑1 (MQ=255)
                                                                                               cGAATTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTATCCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTggg                                                        <  2:1388359/150‑1 (MQ=255)
                                                                                                  cTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTAAGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCg                                                     >  1:548194/1‑150 (MQ=255)
                                                                                                    ttATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCCAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGcc                                                   >  1:1415274/1‑150 (MQ=255)
                                                                                                     tataTCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCa                                                  <  1:1080989/150‑1 (MQ=255)
                                                                                                     tataTCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCGGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTTCCGGAATCGCTTTCGCGGGACGTTTAATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCa                                                  >  2:1399596/1‑150 (MQ=255)
                                                                                                        atCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTACCCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGt                                               >  2:1208364/1‑150 (MQ=255)
                                                                                                        atCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGt                                               >  1:132976/1‑150 (MQ=255)
                                                                                                         tCTTAAGACCGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTg                                              <  2:510675/150‑1 (MQ=255)
                                                                                                            tAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCgc                                           >  2:1701652/1‑149 (MQ=255)
                                                                                                            tAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGa                                           <  2:56956/150‑1 (MQ=255)
                                                                                                             aagaagGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAg                                          <  1:404066/150‑1 (MQ=255)
                                                                                                             aaGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAg                                          <  1:1324509/150‑1 (MQ=255)
                                                                                                              aGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACTATCAAGCAACCTGTACCGGAATCGCGTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAACTAGGCGACGTTGGGTCTCCAGGTGCGAGc                                         >  2:1175517/1‑150 (MQ=255)
                                                                                                              aGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGc                                         >  1:695562/1‑150 (MQ=255)
                                                                                                                aaCGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCTTTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGctt                                       <  1:1349026/150‑1 (MQ=255)
                                                                                                                  cGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGcttct                                     >  2:1581682/1‑150 (MQ=255)
                                                                                                                  cGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGcttct                                     >  2:6185/1‑150 (MQ=255)
                                                                                                                  cGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGcttct                                     <  2:1313897/150‑1 (MQ=255)
                                                                                                                   ggcttCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGcttctt                                    <  1:928314/147‑1 (MQ=255)
                                                                                                                        cGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGCGCTTCTTCATcg                               >  1:177468/1‑149 (MQ=255)
                                                                                                                        cGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCt                               <  1:697602/150‑1 (MQ=255)
                                                                                                                         gCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTg                              <  2:1279178/150‑1 (MQ=255)
                                                                                                                               gaCGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATgg                        <  2:1325559/150‑1 (MQ=255)
                                                                                                                                aCGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGt                       <  2:423956/150‑1 (MQ=255)
                                                                                                                                     gTCTCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGa                  <  2:854854/150‑1 (MQ=255)
                                                                                                                                     gTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGa                  <  1:442527/150‑1 (MQ=255)
                                                                                                                                     cctgcgGCTGGTTACTCACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGa                  >  1:1166893/4‑150 (MQ=255)
                                                                                                                                       tgcgGCTGGTTACTCACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAg                >  2:222605/2‑150 (MQ=255)
                                                                                                                                       cgcgGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAg                >  1:1838636/1‑150 (MQ=255)
                                                                                                                                        gcgGCTGGTTACTCACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGc               >  1:1036614/1‑150 (MQ=255)
                                                                                                                                        gcgGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGc               >  1:858599/1‑150 (MQ=255)
                                                                                                                                         cgGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCt              >  1:1484762/1‑150 (MQ=255)
                                                                                                                                          ggCTGGTTACTCACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTg             >  1:1441008/1‑150 (MQ=255)
                                                                                                                                            cTGGTGAGTAACCAGCAGCAGGGATAACATTCCAGCAACCGCTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATTGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGc           <  2:1688085/150‑1 (MQ=255)
                                                                                                                                              ggTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGTTCGCCAGGGCGGCGTTTCTGCTTCTGGATTGGTAAGGAAGATGGGga         >  1:1425780/1‑150 (MQ=255)
                                                                                                                                              ggTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCga         >  2:1633258/1‑150 (MQ=255)
                                                                                                                                               gTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCgat        <  2:988964/150‑1 (MQ=255)
                                                                                                                                                tGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCgatg       >  1:1859047/1‑150 (MQ=255)
                                                                                                                                                     aaCCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGAc  <  2:1424240/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGTTACTAAAACGACTTTATATCTTAAGAACGGATTCGCTGGAGACGATGTCGCGGCTGGTGAGTAACCAGCCGCAGGGATAACAATCAAGCAACCTGTACCGGAATCGCTTTCGCGGTACGTTTCATTTCATTGTCGCCTTCAAAATAGGCGACGTTGGGTCGCCAGGTGCGAGCTTCTTCATCTGGCATGGTAACGAAGCTGGCGATGATGAC  >  NC_000913/146144‑146442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: