Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,599 T→A 5.7% intergenic (+25/+428) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,5990TA 5.7% 344.2 / 7.0 120intergenic (+25/+428)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base T (59/54);  new base A (3/4);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

Only 100 of 121 total aligned reads displayed.

ATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCA  >  NC_000913/2057452‑2057745
                                                                                                                                                   |                                                                                                                                                  
aTGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc                                                                                                                                                  <  1:1648811/150‑1 (MQ=255)
aTGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc                                                                                                                                                  <  1:1532449/150‑1 (MQ=255)
aTGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTAc                                                                                                                                                  <  2:864137/150‑1 (MQ=255)
      gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                            >  1:1171557/1‑150 (MQ=255)
          aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTc                                                                                                                                        >  2:1716203/1‑150 (MQ=255)
            tcagAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                      <  2:1438618/147‑1 (MQ=255)
             cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTCTCATTACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                     <  1:1410183/150‑1 (MQ=255)
             cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                     <  2:565671/150‑1 (MQ=255)
                aaGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaa                                                                                                                                  <  2:50079/150‑2 (MQ=39)
                aaGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaa                                                                                                                                  <  1:413792/150‑2 (MQ=39)
                 aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtc                                                                                                                                 <  1:677384/150‑1 (MQ=255)
                 aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtc                                                                                                                                 <  2:742148/150‑1 (MQ=255)
                  gTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtct                                                                                                                                >  1:975135/1‑150 (MQ=255)
                     gAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATATCaa                                                                                                                             >  2:62757/1‑149 (MQ=255)
                       gCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCatat                                                                                                                           >  2:1403845/1‑150 (MQ=255)
                          ttCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAt                                                                                                                        <  1:1778374/150‑1 (MQ=255)
                            ccccAGAAGATTTAGAAATCTTTGAACGGCTTGTTGATGCCCTTGAAGAAGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCCAAATATCATTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                                                                      <  1:66197/150‑1 (MQ=255)
                             cccAGAAGATTTAGAAATCTTTGAAGTGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCATATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCt                                                                                                                     <  1:1298924/150‑1 (MQ=255)
                               ctgaagaTTTATAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTta                                                                                                                   <  2:237269/148‑1 (MQ=255)
                                 taagaTTTAGAAATCTTTGAAGGTCTTGTTGATTCCCTTGAAGATGAAGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                 <  1:115702/149‑1 (MQ=255)
                                 gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                 <  1:682551/150‑1 (MQ=255)
                                  aagaTTTAGAAATATTTGAATGTCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaa                                                                                                                <  1:866487/150‑1 (MQ=255)
                                     aTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaa                                                                                                             <  1:1860950/150‑1 (MQ=255)
                                     aTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaa                                                                                                             <  2:86751/150‑1 (MQ=255)
                                         aGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGAGGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                                                                         >  1:1064847/1‑150 (MQ=255)
                                            aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                      >  2:184316/1‑150 (MQ=255)
                                              tCTTTGAAGGGCTTGTTGATGCCCTTGAAGATCACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTATACGGAAGATTTCTTTAATATCATATATTTCTTATAAAAaatataatat                                                                                                    >  1:302169/1‑150 (MQ=255)
                                               cTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTGATAAAAAATATAATAtt                                                                                                   >  1:802151/1‑150 (MQ=255)
                                                tttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                                                                  <  2:1276738/150‑1 (MQ=255)
                                                       ggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGt                                                                                           <  2:1767201/150‑1 (MQ=255)
                                                        gCTAGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                          <  1:406568/150‑1 (MQ=255)
                                                         cTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCa                                                                                         >  2:1603298/1‑150 (MQ=255)
                                                            gttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCatat                                                                                      >  1:258444/1‑150 (MQ=255)
                                                                aTGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGCAATCTGTCTTTACGGCGGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGAt                                                                                  <  2:361002/150‑1 (MQ=255)
                                                                  gCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATta                                                                                <  2:953944/150‑1 (MQ=255)
                                                                    ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                              >  1:1861054/1‑150 (MQ=255)
                                                                    ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                              <  2:1179857/150‑1 (MQ=255)
                                                                    ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                              <  2:511629/150‑1 (MQ=255)
                                                                     cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                                                             >  2:1416248/1‑150 (MQ=255)
                                                                     cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                                                             >  2:951324/1‑150 (MQ=255)
                                                                        gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACCGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTAATATTGATTATAcacc                                                                          >  1:622925/1‑150 (MQ=255)
                                                                           tattacgacgATGTACAAAAAGTTTATCATAACGTAGCATATCTATTATTATATTTTAAAGAAATCTGTCTTTAAGGCAGATTTCTTTAATCTCATATAATTATTATAAAAAATATAATTTTCAACTCTTCATATTGATTATAcccccca                                                                       <  2:1289993/146‑2 (MQ=255)
                                                                           gATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcaccccc                                                                       >  1:87752/1‑150 (MQ=255)
                                                                            aTGACGACGATGTACAAATAGTTTATCATTACGTCGGAAATCTCTAATTATATTTTAAATAAATCTGTCTTTACGGCAGATTTATTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATAATAcgctcacg                                                                      >  2:301160/1‑143 (MQ=255)
                                                                                cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTcc                                                                  <  2:1221725/150‑1 (MQ=255)
                                                                                  acgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCa                                                                <  1:708207/150‑1 (MQ=255)
                                                                                     aTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCagag                                                             <  2:1724517/150‑1 (MQ=255)
                                                                                       gTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATATATCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGATCCCAGAGaa                                                           >  2:1159352/1‑150 (MQ=255)
                                                                                        tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa                                                          >  1:1552679/1‑150 (MQ=255)
                                                                                         aCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCAGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTATACCAACAAGTTACCAGATAaat                                                         >  1:347534/1‑150 (MQ=255)
                                                                                          cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaata                                                        >  2:428215/1‑150 (MQ=255)
                                                                                            aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                      <  2:941918/150‑1 (MQ=255)
                                                                                             aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAataata                                                     <  2:1347952/150‑1 (MQ=255)
                                                                                             aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAataata                                                     >  1:581744/1‑150 (MQ=255)
                                                                                                ttttATCATAACGTCTCAAATCTCTAATTATCTATTAAATTAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAATAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                  <  1:292325/149‑1 (MQ=255)
                                                                                                      cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTGTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACCCACCCGTTACCAGATAAATAATATTTATTaaa                                            >  2:263733/1‑150 (MQ=255)
                                                                                                      cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                            >  1:1866183/1‑150 (MQ=255)
                                                                                                      aaTAACGTCGCAAATCTCTAAATATCTTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                            <  2:1538511/149‑1 (MQ=255)
                                                                                                        tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAAt                                          >  1:373208/1‑150 (MQ=255)
                                                                                                          aCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTc                                        >  2:1860605/1‑150 (MQ=255)
                                                                                                           cGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAATTTcc                                       >  2:1143672/1‑150 (MQ=255)
                                                                                                           agTCGCAAATCTCTAATTATCTTTTAAATAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTcc                                       <  2:1521102/149‑1 (MQ=255)
                                                                                                            gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTATGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCTTTCCAACTAAAATATAATTTTTTaaattacaa                                      >  1:1721917/1‑144 (MQ=255)
                                                                                                             tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg                                     <  2:1695848/150‑1 (MQ=255)
                                                                                                             tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg                                     <  2:590484/150‑1 (MQ=255)
                                                                                                              cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCAGTTCCCAGAGAAATAATATTTATTAAAATTCCAGt                                    >  1:1132820/1‑150 (MQ=255)
                                                                                                               gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTAAACCCACCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAttt                                   >  2:665597/1‑150 (MQ=255)
                                                                                                               gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACAACCCCTTTCCCAGAGAAATAATATTTATTAAAATTCCAGtt                                   >  1:796129/1‑150 (MQ=255)
                                                                                                                cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGtta                                  >  1:626630/1‑149 (MQ=255)
                                                                                                                   aTCTCTAATTATCTTTTAAATAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                               <  1:1697051/150‑1 (MQ=255)
                                                                                                                   aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCTACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                               <  1:1231855/150‑1 (MQ=255)
                                                                                                                   aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                               <  1:1280267/150‑1 (MQ=255)
                                                                                                                     ctctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt                             <  2:802151/150‑1 (MQ=255)
                                                                                                                       ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCtttt                           >  1:791148/1‑150 (MQ=255)
                                                                                                                       ctAATTATATTTTAAAGAAATCTGTCTTTACGGCAGATTTATTTAATCTCATATAATTCATATAAAAAATATAAAATTAAACTCGTCATATTTATTATACCCCCCCGTTCCCAGAGAAATAATAATTATTAAAATTCCAGTTATTAtttt                           >  2:1076703/1‑150 (MQ=255)
                                                                                                                        tAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                          <  1:1872714/150‑1 (MQ=255)
                                                                                                                         aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                         <  1:1716203/150‑1 (MQ=255)
                                                                                                                            tATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa                      >  2:845728/1‑150 (MQ=255)
                                                                                                                             aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAATTATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATTATATTTATTAATATTCCAGTTCTTCTTTTTcgtct                     >  1:307113/1‑146 (MQ=255)
                                                                                                                             aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt                     <  1:1403845/150‑1 (MQ=255)
                                                                                                                              tcatttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATTATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt                    <  2:325163/147‑1 (MQ=255)
                                                                                                                               cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                   <  2:480905/150‑1 (MQ=255)
                                                                                                                               cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                   >  2:546315/1‑150 (MQ=255)
                                                                                                                                ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAACATTCCAGTGCTTCTTTTTCTGATTAc                  >  2:900372/1‑150 (MQ=255)
                                                                                                                                ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                  >  1:1759221/1‑150 (MQ=255)
                                                                                                                                   tAAAGAAATCTGTCTTTACGTCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa               <  2:1146825/150‑1 (MQ=255)
                                                                                                                                   tAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa               >  2:877854/1‑150 (MQ=255)
                                                                                                                                    aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa              >  1:1573997/1‑150 (MQ=255)
                                                                                                                                    aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa              >  2:1389274/1‑150 (MQ=255)
                                                                                                                                    aaaGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCTTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa              >  2:261300/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             <  1:587771/150‑1 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             <  1:1641330/150‑1 (MQ=255)
                                                                                                                                     aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             >  1:1127271/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             >  2:1557979/1‑150 (MQ=255)
                                                                                                                                      aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCTTTTTCTGATTACAGAAgg            >  1:386980/1‑150 (MQ=255)
                                                                                                                                       taaaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGc           <  2:1526577/149‑1 (MQ=255)
                                                                                                                                       taaaTCAGTATTTAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGc           <  1:301160/149‑1 (MQ=255)
                                                                                                                                                cTTTACGGCAGATTTCTTTAATCTCTTATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCa  >  2:788079/1‑150 (MQ=255)
                                                                                                                                                cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAAATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGAAAAGTGGCa  >  2:1772246/1‑150 (MQ=255)
                                                                                                                                                cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCAAGAGAAATAATATTTATTAAAATTCCAGTTATTCTTTTTCTGCTTACAGAAGGAAAAGTggaa  >  1:572371/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCA  >  NC_000913/2057452‑2057745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑