Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,603 G→A 6.6% intergenic (+29/+424) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,6030GA 6.6% 350.4 / 10.2 121intergenic (+29/+424)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base G (60/52);  new base A (3/5);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-02

Only 100 of 124 total aligned reads displayed.

TTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACG  >  NC_000913/2057456‑2057751
                                                                                                                                                   |                                                                                                                                                    
ttGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCa                                                                                                                                                    <  1:24811/150‑1 (MQ=255)
  gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                                  >  1:1171557/1‑150 (MQ=255)
  gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                                  >  2:1641330/1‑150 (MQ=255)
      aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTc                                                                                                                                              >  2:1716203/1‑150 (MQ=255)
        tcagAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                            <  2:1438618/147‑1 (MQ=255)
         cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTCTCATTACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                           <  1:1410183/150‑1 (MQ=255)
         cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                           <  2:565671/150‑1 (MQ=255)
            aaGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaa                                                                                                                                        <  2:50079/150‑2 (MQ=39)
            aaGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaa                                                                                                                                        <  1:413792/150‑2 (MQ=39)
             aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtc                                                                                                                                       <  2:742148/150‑1 (MQ=255)
             aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtc                                                                                                                                       <  1:677384/150‑1 (MQ=255)
              gTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtct                                                                                                                                      >  1:975135/1‑150 (MQ=255)
                      ttCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAt                                                                                                                              <  1:1778374/150‑1 (MQ=255)
                        ccccAGAAGATTTAGAAATCTTTGAACGGCTTGTTGATGCCCTTGAAGAAGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCCAAATATCATTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                                                                            <  1:66197/150‑1 (MQ=255)
                         cccAGAAGATTTAGAAATCTTTGAAGTGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCATATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCt                                                                                                                           <  1:1298924/150‑1 (MQ=255)
                         cccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCt                                                                                                                           >  2:587580/1‑150 (MQ=255)
                          ccAGAAGATTTTGAAATCTTTGAAGGGCTTGTTGATGCCCGTTAATATGTAGACGATGTTCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGTTTTCTTTAATCTCATATAATTCtt                                                                                                                          <  2:1164193/150‑1 (MQ=255)
                           ctgaagaTTTATAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTta                                                                                                                         <  2:237269/148‑1 (MQ=255)
                             taagaTTTAGAAATCTTTGAAGGTCTTGTTGATTCCCTTGAAGATGAAGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                       <  1:115702/149‑1 (MQ=255)
                                gaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaa                                                                                                                    >  1:1526577/1‑150 (MQ=255)
                                 aTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaa                                                                                                                   <  2:86751/150‑1 (MQ=255)
                                     aGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGAGGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatat                                                                                                               >  1:1064847/1‑150 (MQ=255)
                                        aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                            >  2:184316/1‑150 (MQ=255)
                                          tCTTTGAAGGGCTTGTTGATGCCCTTGAAGATCACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTATACGGAAGATTTCTTTAATATCATATATTTCTTATAAAAaatataatat                                                                                                          >  1:302169/1‑150 (MQ=255)
                                           cTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTTTCTTTTAAAGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATAtt                                                                                                         >  1:316739/1‑150 (MQ=255)
                                           cTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTGATAAAAAATATAATAtt                                                                                                         >  1:802151/1‑150 (MQ=255)
                                            tttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATAtta                                                                                                        >  1:953292/1‑149 (MQ=255)
                                                aaGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACt                                                                                                    >  2:1803116/1‑150 (MQ=255)
                                                   ggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGt                                                                                                 <  2:1767201/150‑1 (MQ=255)
                                                    gCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                                <  2:449028/150‑1 (MQ=255)
                                                    gCTAGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                                <  1:406568/150‑1 (MQ=255)
                                                     cTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCa                                                                                               >  2:1603298/1‑150 (MQ=255)
                                                        gttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                                                                            >  1:641649/1‑150 (MQ=255)
                                                          tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTg                                                                                          >  2:1469697/1‑150 (MQ=255)
                                                           gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGa                                                                                         >  2:1364480/1‑150 (MQ=255)
                                                            aTGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGCAATCTGTCTTTACGGCGGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGAt                                                                                        <  2:361002/150‑1 (MQ=255)
                                                              gCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATta                                                                                      <  2:953944/150‑1 (MQ=255)
                                                                ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                                    <  2:1179857/150‑1 (MQ=255)
                                                                ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtata                                                                                    >  1:1861054/1‑150 (MQ=255)
                                                                 cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                                                                   >  2:951324/1‑150 (MQ=255)
                                                                 cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                                                                   >  2:1416248/1‑150 (MQ=255)
                                                                      aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccc                                                                              >  2:801414/1‑150 (MQ=255)
                                                                       tattacgacgATGTACAAAAAGTTTATCATAACGTAGCATATCTATTATTATATTTTAAAGAAATCTGTCTTTAAGGCAGATTTCTTTAATCTCATATAATTATTATAAAAAATATAATTTTCAACTCTTCATATTGATTATAcccccca                                                                             <  2:1289993/146‑2 (MQ=255)
                                                                       gATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcaccccc                                                                             >  1:87752/1‑150 (MQ=255)
                                                                        aTGACGACGATGTACAAATAGTTTATCATTACGTCGGAAATCTCTAATTATATTTTAAATAAATCTGTCTTTACGGCAGATTTATTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATAATAcgctcacg                                                                            >  2:301160/1‑143 (MQ=255)
                                                                            cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTcc                                                                        <  2:1221725/150‑1 (MQ=255)
                                                                              acgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCa                                                                      <  1:708207/150‑1 (MQ=255)
                                                                                 aTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCagag                                                                   <  2:1724517/150‑1 (MQ=255)
                                                                                   gTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATATATCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGATCCCAGAGaa                                                                 >  2:1159352/1‑150 (MQ=255)
                                                                                    tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa                                                                >  1:1552679/1‑150 (MQ=255)
                                                                                      cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaata                                                              >  2:428215/1‑150 (MQ=255)
                                                                                        aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                            <  2:941918/150‑1 (MQ=255)
                                                                                         aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAataata                                                           <  2:1347952/150‑1 (MQ=255)
                                                                                         aaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAataata                                                           >  1:581744/1‑150 (MQ=255)
                                                                                            ttttATCATAACGTCTCAAATCTCTAATTATCTATTAAATTAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAATAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                        <  1:292325/149‑1 (MQ=255)
                                                                                                 tCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCAAGAGAAATAATATTTATTaa                                                   >  1:1808101/1‑150 (MQ=255)
                                                                                                  cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTGTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACCCACCCGTTACCAGATAAATAATATTTATTaaa                                                  >  2:263733/1‑150 (MQ=255)
                                                                                                  cATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                                  >  1:1866183/1‑150 (MQ=255)
                                                                                                  aaTAACGTCGCAAATCTCTAAATATCTTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTaaa                                                  <  2:1538511/149‑1 (MQ=255)
                                                                                                    tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAAt                                                >  1:373208/1‑150 (MQ=255)
                                                                                                      aCGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTc                                              >  2:1860605/1‑150 (MQ=255)
                                                                                                       agTCGCAAATCTCTAATTATCTTTTAAATAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTcc                                             <  2:1521102/149‑1 (MQ=255)
                                                                                                         tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg                                           <  2:590484/150‑1 (MQ=255)
                                                                                                          cGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCAGTTCCCAGAGAAATAATATTTATTAAAATTCCAGt                                          >  1:1132820/1‑150 (MQ=255)
                                                                                                           gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTAAACCCACCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAttt                                         >  2:665597/1‑150 (MQ=255)
                                                                                                           gCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACAACCCCTTTCCCAGAGAAATAATATTTATTAAAATTCCAGtt                                         >  1:796129/1‑150 (MQ=255)
                                                                                                            cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGtta                                        >  1:626630/1‑149 (MQ=255)
                                                                                                              aaTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACCAGTTCCCAGAGAAATAATATTTATTAAAATTCAAGttctt                                      >  2:277292/1‑150 (MQ=255)
                                                                                                               aTCTCTAATTATCTTTTAAATAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                     <  1:1697051/150‑1 (MQ=255)
                                                                                                               aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCTACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                     <  1:1231855/150‑1 (MQ=255)
                                                                                                               aTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc                                     <  1:1280267/150‑1 (MQ=255)
                                                                                                                   ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCtttt                                 >  1:791148/1‑150 (MQ=255)
                                                                                                                   ctAATTATATTTTAAAGAAATCTGTCTTTACGGCAGATTTATTTAATCTCATATAATTCATATAAAAAATATAAAATTAAACTCGTCATATTTATTATACCCCCCCGTTCCCAGAGAAATAATAATTATTAAAATTCCAGTTATTAtttt                                 >  2:1076703/1‑150 (MQ=255)
                                                                                                                    tAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                                <  1:1872714/150‑1 (MQ=255)
                                                                                                                     aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                               <  1:1716203/150‑1 (MQ=255)
                                                                                                                        tATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa                            >  2:845728/1‑150 (MQ=255)
                                                                                                                         aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAATTATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATTATATTTATTAATATTCCAGTTCTTCTTTTTcgtct                           >  1:307113/1‑146 (MQ=255)
                                                                                                                          tcatttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATTATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt                          <  2:325163/147‑1 (MQ=255)
                                                                                                                           cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                         >  2:546315/1‑150 (MQ=255)
                                                                                                                           cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                         <  2:480905/150‑1 (MQ=255)
                                                                                                                            ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAACATTCCAGTGCTTCTTTTTCTGATTAc                        >  2:900372/1‑150 (MQ=255)
                                                                                                                            ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                        >  1:1759221/1‑150 (MQ=255)
                                                                                                                             tttAAAGAAATATGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa                       <  1:1658790/150‑1 (MQ=255)
                                                                                                                               tAAAGAAATCTGTCTTTACGTCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                     <  2:1146825/150‑1 (MQ=255)
                                                                                                                               tAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                     >  2:877854/1‑150 (MQ=255)
                                                                                                                                aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa                    >  2:1389274/1‑150 (MQ=255)
                                                                                                                                 aaGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                   <  1:587771/150‑1 (MQ=255)
                                                                                                                                 aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                   <  1:1641330/150‑1 (MQ=255)
                                                                                                                                 aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                   >  1:1127271/1‑150 (MQ=255)
                                                                                                                                 aaGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg                   >  2:1557979/1‑150 (MQ=255)
                                                                                                                                  aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCTTTTTCTGATTACAGAAgg                  >  1:386980/1‑150 (MQ=255)
                                                                                                                                   taaaTCAGTATTTAAGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGc                 <  1:301160/149‑1 (MQ=255)
                                                                                                                                            cTTTACGGCAGATTTCTTTAATCTCTTATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCa        >  2:788079/1‑150 (MQ=255)
                                                                                                                                            cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCAAGAGAAATAATATTTATTAAAATTCCAGTTATTCTTTTTCTGCTTACAGAAGGAAAAGTggaa        >  1:572371/1‑148 (MQ=255)
                                                                                                                                                aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTa    <  2:1698753/150‑1 (MQ=255)
                                                                                                                                                 cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTAc   <  1:1364480/150‑1 (MQ=255)
                                                                                                                                                 cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTAc   >  1:1411959/1‑150 (MQ=255)
                                                                                                                                                  ggCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACg  >  2:832609/1‑150 (MQ=255)
                                                                                                                                                  ggCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACg  <  1:184316/150‑1 (MQ=255)
                                                                                                                                                  ggCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGAGGCAATTACg  >  1:568215/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACG  >  NC_000913/2057456‑2057751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: