Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,126,154 A→T 5.7% I23I (ATT→ATA) ‡ wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,126,1540AT 5.7% 349.6 / 6.9 117I23I (ATT→ATA) ‡wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base T (3/4);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 120 total aligned reads displayed.

CGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTG  >  NC_000913/2126005‑2126302
                                                                                                                                                     |                                                                                                                                                    
  cAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGGTGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAAt                                                                                                                                                    <  2:73483/150‑1 (MQ=255)
   aGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAACCTCGCCGCGACTGTTCTCGACaaga                                                                                                                                                   >  1:1625824/1‑148 (MQ=255)
    gCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATaa                                                                                                                                                  <  2:83134/150‑1 (MQ=255)
      cccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCCGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCGCAAACCAGTTACCATGCGCCGGGCGGAGGGTTATTTTGCCAAGCAGAAACTCGCCGCGACAGTTCTCGCCAATAAAg                                                                                                                                                >  2:1453982/1‑150 (MQ=255)
           ttCCGCCATCGTCATCCGCTCAACTGCTGCTTCCAGCGTTTCGTCTTTCTTCACGCGCCCACCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCAAAGCAGAAACTCGCCGCGACTGATCTCGACAATAAAGTCgag                                                                                                                                           <  2:1120019/150‑1 (MQ=255)
            tCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgaga                                                                                                                                          <  1:998785/150‑1 (MQ=255)
             ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                         <  2:1833650/150‑1 (MQ=255)
             ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                         <  2:1648383/150‑1 (MQ=255)
             ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                         <  1:1731657/150‑1 (MQ=255)
              cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagaga                                                                                                                                        <  1:1586072/150‑1 (MQ=255)
               gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                       <  1:45159/150‑4 (MQ=255)
               gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                       <  1:1384602/150‑4 (MQ=255)
               gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTATAAAGTCgagagag                                                                                                                                       >  2:949719/1‑150 (MQ=255)
                ccATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaacag                                                                                                                                      <  1:850631/150‑5 (MQ=255)
                  cgcggccgCCGCTCAAAGGCGGCTTCCAGCGGTTCGTCTTTCAGCACGCGCCCTCCCGGCACCAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCACGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACa                                                                                                                                    <  2:469415/143‑1 (MQ=255)
                    cGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAg                                                                                                                                  <  2:925/150‑1 (MQ=255)
                       cAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGCGAGAGAGACAAGCgg                                                                                                                               >  1:634069/1‑150 (MQ=255)
                         gCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                             <  1:245608/150‑1 (MQ=255)
                         gCCGCGCAAATGCGGCTTCCAGCGGTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                             <  1:1623615/150‑1 (MQ=255)
                           cGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTg                                                                                                                           >  2:1630389/1‑150 (MQ=255)
                              tCAAATTCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACGAGCGGAGTGGAg                                                                                                                        <  2:1619996/150‑1 (MQ=255)
                               cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                       >  2:9080/1‑150 (MQ=255)
                               cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                       <  2:217997/150‑1 (MQ=255)
                                   tGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCAc                                                                                                                   >  1:991898/1‑150 (MQ=255)
                                     cGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTa                                                                                                                 >  1:45561/1‑150 (MQ=255)
                                       gCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTAcc                                                                                                               >  1:1663087/1‑150 (MQ=255)
                                        cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCg                                                                                                              <  1:1841670/150‑1 (MQ=255)
                                        cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGCGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGACGAGAGAGACAAGCGGAGTGGAGCGCACTACCg                                                                                                              >  2:1836191/1‑150 (MQ=255)
                                         ttCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGt                                                                                                             <  1:881063/150‑1 (MQ=255)
                                          tCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTg                                                                                                            <  1:1291319/150‑1 (MQ=255)
                                           ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCCCAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                           >  1:1005518/1‑150 (MQ=255)
                                           ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                           <  2:228335/150‑1 (MQ=255)
                                           ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTATTTTGCCAAGCAGCAACTCGCCGCGCCTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                           >  2:1216818/1‑150 (MQ=255)
                                            cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGCGCGCACTACCGTGGc                                                                                                          >  1:91239/1‑150 (MQ=255)
                                            cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGc                                                                                                          <  1:998841/150‑1 (MQ=255)
                                              gCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaa                                                                                                        <  2:1574427/150‑1 (MQ=255)
                                                gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                      <  1:579896/150‑1 (MQ=255)
                                                gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                      <  2:1048643/150‑1 (MQ=255)
                                                 tttCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGAGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGt                                                                                                     >  1:1478181/1‑150 (MQ=255)
                                                     gTCTTTCTGCACGCGCCCTCCCGGTACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTc                                                                                                 >  1:461097/1‑150 (MQ=255)
                                                       cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACATGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                               >  2:900052/1‑150 (MQ=255)
                                                       cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCCt                                                                                               >  1:1533590/1‑150 (MQ=255)
                                                        tttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTAGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTg                                                                                              >  2:1282176/1‑150 (MQ=255)
                                                           cTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACg                                                                                           <  1:225345/150‑1 (MQ=255)
                                                           cTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACg                                                                                           <  2:1266029/150‑1 (MQ=255)
                                                            tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                          >  1:1128584/1‑150 (MQ=255)
                                                            tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                          >  2:1608298/1‑150 (MQ=255)
                                                              cACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaa                                                                                        <  2:658209/150‑1 (MQ=255)
                                                                 gcgcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaaaaa                                                                                     <  1:702120/150‑1 (MQ=255)
                                                                   gcCCTCCCGGCACAACCAAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAca                                                                                   >  1:1374556/1‑150 (MQ=255)
                                                                   gcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAca                                                                                   <  2:1083834/150‑1 (MQ=255)
                                                                     ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAACCTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatc                                                                                 <  2:1562294/150‑1 (MQ=255)
                                                                     ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCGCGACAATAAAGTCGAGAGAGACACGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCGTGACGTaaaagcctc                                                                                 >  2:1713515/1‑145 (MQ=255)
                                                                      cTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatca                                                                                >  2:1431518/1‑150 (MQ=255)
                                                                        cccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                              >  1:1516419/1‑150 (MQ=255)
                                                                         ccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAACCATCATTa                                                                             >  1:307350/1‑150 (MQ=255)
                                                                          cGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGCGCGCACTACCGTGGCAAAGTCTTCCTGACGTAACAACATCATTAc                                                                            >  2:1828498/1‑150 (MQ=255)
                                                                               caACCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGACAAAGTCTTCCGGACGGAAAAACAGCATTACCCCCg                                                                       >  2:209706/1‑150 (MQ=255)
                                                                                aaaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAATCATCATTACCCCCGa                                                                      >  2:371130/1‑150 (MQ=255)
                                                                                 aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGaa                                                                     >  1:321397/1‑150 (MQ=255)
                                                                                 aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAATACCTCATTCCCCCCGaa                                                                     >  2:1161810/1‑150 (MQ=255)
                                                                                   ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAg                                                                   >  2:1361310/1‑150 (MQ=255)
                                                                                   ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGACGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTTCCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGAAAg                                                                   >  2:1451391/1‑150 (MQ=255)
                                                                                     aGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                 >  2:72892/1‑150 (MQ=255)
                                                                                     aGTAAACCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGAGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                 >  2:543083/1‑150 (MQ=255)
                                                                                         aCCCTGCGCCGGGCGGTTTGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                             <  2:820517/150‑1 (MQ=255)
                                                                                          cccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAGACATCATTACCCCCGAAAGCGGTCtt                                                            >  1:1441214/1‑150 (MQ=255)
                                                                                          cccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCtt                                                            <  1:778616/150‑1 (MQ=255)
                                                                                           ccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGGGACAAGCGGAGTGGAGCGCCCTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTg                                                           >  1:1736687/1‑150 (MQ=255)
                                                                                             tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                         <  2:1835267/150‑1 (MQ=255)
                                                                                                   gggCGGGTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCaa                                                   <  1:950070/150‑1 (MQ=255)
                                                                                                    ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAGAAACATCATTACCCCCGAAAGCGGTCTTGATTCGCact                                                  >  2:1740299/1‑148 (MQ=255)
                                                                                                    ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                  <  1:1531493/150‑1 (MQ=255)
                                                                                                    ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                  >  1:1746671/1‑150 (MQ=255)
                                                                                                      gggttggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                <  2:991898/149‑1 (MQ=255)
                                                                                                      cGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                >  2:938200/1‑150 (MQ=255)
                                                                                                                ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTa                                      >  1:1481765/1‑150 (MQ=255)
                                                                                                                ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGACTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAAATCATTACCCCCGAAAGAGGTCTTGATTCTCAACGCACCACTGGTa                                      >  1:1592475/1‑150 (MQ=255)
                                                                                                                    gCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTg                                  >  1:603023/1‑150 (MQ=255)
                                                                                                                      cAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGca                                <  1:1180906/150‑2 (MQ=255)
                                                                                                                       aaGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTg                               <  1:1301834/150‑1 (MQ=255)
                                                                                                                         gCAGAAAGTCGCCGCGTGTGTTCTCGACAATAAATTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCTTTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                             <  1:1187190/150‑1 (MQ=255)
                                                                                                                         gCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                             <  1:1066968/150‑1 (MQ=255)
                                                                                                                          cAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAGTCTCAAGGAACCACTGGTAAGTGCTGGCa                            >  2:1276003/1‑150 (MQ=255)
                                                                                                                            gAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAACTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAg                          >  2:1126899/1‑150 (MQ=255)
                                                                                                                                   gcagcgACTGTTCTCGACAATAAAGACGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGGCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                   <  1:65943/147‑1 (MQ=255)
                                                                                                                                   cccgcGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                   <  2:1270809/149‑1 (MQ=255)
                                                                                                                                     cgcgACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTc                 >  1:1751449/1‑150 (MQ=255)
                                                                                                                                        gACTGGTCTCGACAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGGGGCAAAGTCTGCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAg              <  1:886852/150‑1 (MQ=255)
                                                                                                                                         gtctctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt             >  2:1873886/6‑150 (MQ=255)
                                                                                                                                          cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTg            <  2:8322/150‑1 (MQ=255)
                                                                                                                                            tctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag          >  1:509440/3‑150 (MQ=255)
                                                                                                                                            gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag          >  2:1716758/1‑150 (MQ=255)
                                                                                                                                            gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag          <  2:1663087/150‑1 (MQ=255)
                                                                                                                                               ctcGACAATAAAGTCGAGAGAGACAAGCGGAGTGGATCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACACCCGAAAGCGGACTTGATTCTCAAGGAACCACTGGTAAGTGCTGGAAAGCCCCGGTTCCAGTGAGCtt       >  2:1351501/1‑150 (MQ=255)
                                                                                                                                                 cGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTc     >  2:1268284/1‑150 (MQ=255)
                                                                                                                                                   ccAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTGATTCTCAAGGAACACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg  <  2:928188/149‑1 (MQ=255)
                                                                                                                                                   aCAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGt   <  2:461097/150‑1 (MQ=255)
                                                                                                                                                    cAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg  <  1:214666/150‑1 (MQ=255)
                                                                                                                                                    aaaTAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg  <  1:430214/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCAGCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTG  >  NC_000913/2126005‑2126302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: