Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,126,158 A→T 5.1% F22Y (TTT→TAT) ‡ wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,126,1580AT 5.1% 339.6 / 6.8 117F22Y (TTT→TAT) ‡wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (56/55);  new base T (2/4);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 120 total aligned reads displayed.

GCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126009‑2126307
                                                                                                                                                     |                                                                                                                                                     
gCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATaa                                                                                                                                                       <  2:83134/150‑1 (MQ=255)
  cccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCCGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCGCAAACCAGTTACCATGCGCCGGGCGGAGGGTTATTTTGCCAAGCAGAAACTCGCCGCGACAGTTCTCGCCAATAAAg                                                                                                                                                     >  2:1453982/1‑150 (MQ=255)
       ttCCGCCATCGTCATCCGCTCAACTGCTGCTTCCAGCGTTTCGTCTTTCTTCACGCGCCCACCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCAAAGCAGAAACTCGCCGCGACTGATCTCGACAATAAAGTCgag                                                                                                                                                <  2:1120019/150‑1 (MQ=255)
        tCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgaga                                                                                                                                               <  1:998785/150‑1 (MQ=255)
         ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                              <  2:1833650/150‑1 (MQ=255)
          cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagaga                                                                                                                                             <  1:1586072/150‑1 (MQ=255)
           gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                            <  1:45159/150‑4 (MQ=255)
           gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                            <  1:1384602/150‑4 (MQ=255)
           gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTATAAAGTCgagagag                                                                                                                                            >  2:949719/1‑150 (MQ=255)
            ccATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaacag                                                                                                                                           <  1:850631/150‑5 (MQ=255)
              cgcggccgCCGCTCAAAGGCGGCTTCCAGCGGTTCGTCTTTCAGCACGCGCCCTCCCGGCACCAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCACGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACa                                                                                                                                         <  2:469415/143‑1 (MQ=255)
                cGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAg                                                                                                                                       <  2:925/150‑1 (MQ=255)
                   cAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGCGAGAGAGACAAGCgg                                                                                                                                    >  1:634069/1‑150 (MQ=255)
                     gCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  >  1:427346/1‑150 (MQ=255)
                     gCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  <  1:245608/150‑1 (MQ=255)
                     gCCGCGCAAATGCGGCTTCCAGCGGTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  <  1:1623615/150‑1 (MQ=255)
                       cGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTg                                                                                                                                >  2:1630389/1‑150 (MQ=255)
                        gCTCAAATCCGGCTTCCAGAGTTTATTCTTTCTGCAAGCTACCTACAGGCAAAAACCACTAACCCAGCGCCGGGCGGTTGGTTCTTTTGCCAAGCATAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTgg                                                                                                                               >  2:1519485/1‑150 (MQ=255)
                          tCAAATTCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACGAGCGGAGTGGAg                                                                                                                             <  2:1619996/150‑1 (MQ=255)
                          tCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGAAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAg                                                                                                                             <  1:715815/150‑1 (MQ=255)
                           cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                            <  2:217997/150‑1 (MQ=255)
                           cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                            >  2:9080/1‑150 (MQ=255)
                               tGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCAc                                                                                                                        >  1:991898/1‑150 (MQ=255)
                                 cGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTa                                                                                                                      >  1:45561/1‑150 (MQ=255)
                                   gCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTAcc                                                                                                                    >  1:1663087/1‑150 (MQ=255)
                                    cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCg                                                                                                                   <  1:1841670/150‑1 (MQ=255)
                                    cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGCGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGACGAGAGAGACAAGCGGAGTGGAGCGCACTACCg                                                                                                                   >  2:1836191/1‑150 (MQ=255)
                                     ttCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGt                                                                                                                  <  1:881063/150‑1 (MQ=255)
                                      tCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTg                                                                                                                 <  1:1291319/150‑1 (MQ=255)
                                       ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCCCAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                >  1:1005518/1‑150 (MQ=255)
                                       ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                <  2:228335/150‑1 (MQ=255)
                                       ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTATTTTGCCAAGCAGCAACTCGCCGCGCCTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                >  2:1216818/1‑150 (MQ=255)
                                        cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGCGCGCACTACCGTGGc                                                                                                               >  1:91239/1‑150 (MQ=255)
                                        cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGc                                                                                                               <  1:998841/150‑1 (MQ=255)
                                          gCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaa                                                                                                             <  2:1574427/150‑1 (MQ=255)
                                            gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                           <  2:1048643/150‑1 (MQ=255)
                                             tttCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGAGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGt                                                                                                          >  1:1478181/1‑150 (MQ=255)
                                                 gTCTTTCTGCACGCGCCCTCCCGGTACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTc                                                                                                      >  1:461097/1‑150 (MQ=255)
                                                   cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACATGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                                    >  2:900052/1‑150 (MQ=255)
                                                   cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCCt                                                                                                    >  1:1533590/1‑150 (MQ=255)
                                                    tttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTAGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTg                                                                                                   >  2:1282176/1‑150 (MQ=255)
                                                     ttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGa                                                                                                  >  1:1853659/1‑150 (MQ=255)
                                                       cTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACg                                                                                                <  1:225345/150‑1 (MQ=255)
                                                        tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                               >  1:1128584/1‑150 (MQ=255)
                                                        tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                               >  2:1608298/1‑150 (MQ=255)
                                                          cACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaa                                                                                             <  2:658209/150‑1 (MQ=255)
                                                             gcgcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaaaaa                                                                                          <  1:702120/150‑1 (MQ=255)
                                                               gcCCTCCCGGCACAACCAAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAca                                                                                        >  1:1374556/1‑150 (MQ=255)
                                                                 ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCGCGACAATAAAGTCGAGAGAGACACGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCGTGACGTaaaagcctc                                                                                      >  2:1713515/1‑145 (MQ=255)
                                                                  cTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatca                                                                                     >  2:1431518/1‑150 (MQ=255)
                                                                    cccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                   >  1:1516419/1‑150 (MQ=255)
                                                                     ccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAACCATCATTa                                                                                  >  1:307350/1‑150 (MQ=255)
                                                                      cGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGCGCGCACTACCGTGGCAAAGTCTTCCTGACGTAACAACATCATTAc                                                                                 >  2:1828498/1‑150 (MQ=255)
                                                                           caACCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGACAAAGTCTTCCGGACGGAAAAACAGCATTACCCCCg                                                                            >  2:209706/1‑150 (MQ=255)
                                                                            aaaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAATCATCATTACCCCCGa                                                                           >  2:371130/1‑150 (MQ=255)
                                                                             aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGaa                                                                          >  1:321397/1‑150 (MQ=255)
                                                                             aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAATACCTCATTCCCCCCGaa                                                                          >  2:1161810/1‑150 (MQ=255)
                                                                               ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAg                                                                        >  2:1361310/1‑150 (MQ=255)
                                                                               ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGACGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTTCCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGAAAg                                                                        >  2:1451391/1‑150 (MQ=255)
                                                                                 aGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                      >  2:72892/1‑150 (MQ=255)
                                                                                 aGTAAACCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGAGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                      >  2:543083/1‑150 (MQ=255)
                                                                                  gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCgg                                                                     <  2:1055746/150‑1 (MQ=255)
                                                                                     aCCCTGCGCCGGGCGGTTTGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                                  <  2:820517/150‑1 (MQ=255)
                                                                                      cccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAGACATCATTACCCCCGAAAGCGGTCtt                                                                 >  1:1441214/1‑150 (MQ=255)
                                                                                       ccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGGGACAAGCGGAGTGGAGCGCCCTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTg                                                                >  1:1736687/1‑150 (MQ=255)
                                                                                         tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                              <  2:1835267/150‑1 (MQ=255)
                                                                                           cgcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGATAGCGGTCGGGATtc                                                            >  2:1639527/1‑150 (MQ=255)
                                                                                                ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAGAAACATCATTACCCCCGAAAGCGGTCTTGATTCGCact                                                       >  2:1740299/1‑148 (MQ=255)
                                                                                                ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                       <  1:1711867/150‑1 (MQ=255)
                                                                                                ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                       >  1:1746671/1‑150 (MQ=255)
                                                                                                ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                       <  1:1531493/150‑1 (MQ=255)
                                                                                                  gggttggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                     <  2:991898/149‑1 (MQ=255)
                                                                                                  cGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                     >  2:938200/1‑150 (MQ=255)
                                                                                                        gttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCCCt                                               >  1:528298/1‑150 (MQ=255)
                                                                                                            ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTa                                           >  1:1481765/1‑150 (MQ=255)
                                                                                                            ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGACTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAAATCATTACCCCCGAAAGAGGTCTTGATTCTCAACGCACCACTGGTa                                           >  1:1592475/1‑150 (MQ=255)
                                                                                                                gCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTg                                       >  1:603023/1‑150 (MQ=255)
                                                                                                                  cAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGca                                     <  1:1180906/150‑2 (MQ=255)
                                                                                                                   aaGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTg                                    <  1:1301834/150‑1 (MQ=255)
                                                                                                                     gCAGAAAGTCGCCGCGTGTGTTCTCGACAATAAATTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCTTTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  <  1:1187190/150‑1 (MQ=255)
                                                                                                                     gCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  <  1:1066968/150‑1 (MQ=255)
                                                                                                                      cAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAGTCTCAAGGAACCACTGGTAAGTGCTGGCa                                 >  2:1276003/1‑150 (MQ=255)
                                                                                                                        gAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAACTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAg                               >  2:1126899/1‑150 (MQ=255)
                                                                                                                              cgccgcGACTGATCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGc                         <  2:1284861/150‑1 (MQ=255)
                                                                                                                               gcagcgACTGTTCTCGACAATAAAGACGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGGCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                        <  1:65943/147‑1 (MQ=255)
                                                                                                                               cccgcGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                        <  2:1270809/149‑1 (MQ=255)
                                                                                                                                 cgcgACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTc                      >  1:1751449/1‑150 (MQ=255)
                                                                                                                                    gACTGGTCTCGACAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGGGGCAAAGTCTGCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAg                   <  1:886852/150‑1 (MQ=255)
                                                                                                                                     gtctctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt                  >  2:1873886/6‑150 (MQ=255)
                                                                                                                                     aCTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt                  <  1:949719/150‑1 (MQ=255)
                                                                                                                                      cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTg                 <  2:8322/150‑1 (MQ=255)
                                                                                                                                        tctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               >  1:509440/3‑150 (MQ=255)
                                                                                                                                        gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               <  2:1663087/150‑1 (MQ=255)
                                                                                                                                        gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               >  2:1716758/1‑150 (MQ=255)
                                                                                                                                             cGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTc          >  2:1268284/1‑150 (MQ=255)
                                                                                                                                             cGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATACTCAAAGAACCACTGGTAAGTGCTGGCTAGCCGCGCTTCCAGTGAGAttta          >  2:466667/1‑149 (MQ=255)
                                                                                                                                               ccAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTGATTCTCAAGGAA‑CACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg       <  2:928188/149‑1 (MQ=255)
                                                                                                                                               aCAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGt        <  2:461097/150‑1 (MQ=255)
                                                                                                                                                    aaaGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATAc   >  2:1167597/1‑150 (MQ=255)
                                                                                                                                                     aaGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGCTCTTGAATCTCAAGGAACCACTGGTAAGTGCTGGCATGCCCCGCTTCCAGTGAGAGTTCGTGATAcc  >  2:1709276/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126009‑2126307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑