Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,126,159 A→T 5.5% F22I (TTT→ATT) ‡ wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,126,1590AT 5.5% 334.6 / 5.2 117F22I (TTT→ATT) ‡wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (56/54);  new base T (2/5);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 119 total aligned reads displayed.

CCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126011‑2126307
                                                                                                                                                    |                                                                                                                                                    
   aGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCg                                                                                                                                                  <  2:735356/150‑1 (MQ=255)
     ttCCGCCATCGTCATCCGCTCAACTGCTGCTTCCAGCGTTTCGTCTTTCTTCACGCGCCCACCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCAAAGCAGAAACTCGCCGCGACTGATCTCGACAATAAAGTCgag                                                                                                                                                <  2:1120019/150‑1 (MQ=255)
      tCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgaga                                                                                                                                               <  1:998785/150‑1 (MQ=255)
       ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                              <  2:1648383/150‑1 (MQ=255)
       ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                              <  1:1731657/150‑1 (MQ=255)
       ccGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagag                                                                                                                                              <  2:1833650/150‑1 (MQ=255)
         gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                            <  1:45159/150‑4 (MQ=255)
         gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                            <  1:1384602/150‑4 (MQ=255)
         gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTATAAAGTCgagagag                                                                                                                                            >  2:949719/1‑150 (MQ=255)
          ccATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaacag                                                                                                                                           <  1:850631/150‑5 (MQ=255)
            cgcggccgCCGCTCAAAGGCGGCTTCCAGCGGTTCGTCTTTCAGCACGCGCCCTCCCGGCACCAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCACGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACa                                                                                                                                         <  2:469415/143‑1 (MQ=255)
              cGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAg                                                                                                                                       <  2:925/150‑1 (MQ=255)
                   gCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  >  1:427346/1‑150 (MQ=255)
                   gCCGCGCAAATGCGGCTTCCAGCGGTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  <  1:1623615/150‑1 (MQ=255)
                      gCTCAAATCCGGCTTCCAGAGTTTATTCTTTCTGCAAGCTACCTACAGGCAAAAACCACTAACCCAGCGCCGGGCGGTTGGTTCTTTTGCCAAGCATAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTgg                                                                                                                               >  2:1519485/1‑150 (MQ=255)
                        tCAAATTCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACGAGCGGAGTGGAg                                                                                                                             <  2:1619996/150‑1 (MQ=255)
                        tCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGAAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAg                                                                                                                             <  1:715815/150‑1 (MQ=255)
                         cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                            >  2:9080/1‑150 (MQ=255)
                         cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                            <  2:217997/150‑1 (MQ=255)
                             tGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCAc                                                                                                                        >  1:991898/1‑150 (MQ=255)
                                 gCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTAcc                                                                                                                    >  1:1663087/1‑150 (MQ=255)
                                  cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCg                                                                                                                   <  1:1841670/150‑1 (MQ=255)
                                   ttCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGt                                                                                                                  <  1:881063/150‑1 (MQ=255)
                                     ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCCCAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                >  1:1005518/1‑150 (MQ=255)
                                     ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                <  2:228335/150‑1 (MQ=255)
                                     ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTATTTTGCCAAGCAGCAACTCGCCGCGCCTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                >  2:1216818/1‑150 (MQ=255)
                                      cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGCGCGCACTACCGTGGc                                                                                                               >  1:91239/1‑150 (MQ=255)
                                       aGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCa                                                                                                              <  2:197866/150‑1 (MQ=255)
                                        gCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaa                                                                                                             <  2:1574427/150‑1 (MQ=255)
                                          gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                           <  2:1048643/150‑1 (MQ=255)
                                          gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                           <  1:579896/150‑1 (MQ=255)
                                               gTCTTTCTGCACGCGCCCTCCCGGTACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTc                                                                                                      >  1:461097/1‑150 (MQ=255)
                                                 cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACATGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                                    >  2:900052/1‑150 (MQ=255)
                                                 cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCCt                                                                                                    >  1:1533590/1‑150 (MQ=255)
                                                 cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                                    <  1:1253851/150‑1 (MQ=255)
                                                  tttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTAGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTg                                                                                                   >  2:1282176/1‑150 (MQ=255)
                                                   ttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGa                                                                                                  >  1:1853659/1‑150 (MQ=255)
                                                     cTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACg                                                                                                <  2:1266029/150‑1 (MQ=255)
                                                     cTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACg                                                                                                <  1:225345/150‑1 (MQ=255)
                                                      tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                               >  1:1128584/1‑150 (MQ=255)
                                                      tGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGt                                                                                               >  2:1608298/1‑150 (MQ=255)
                                                        cACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaa                                                                                             <  2:658209/150‑1 (MQ=255)
                                                           gcgcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaaaaa                                                                                          <  1:702120/150‑1 (MQ=255)
                                                             gcCCTCCCGGCACAACCAAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACCAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAca                                                                                        >  1:1374556/1‑150 (MQ=255)
                                                             gcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAca                                                                                        <  2:1083834/150‑1 (MQ=255)
                                                               ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAACCTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatc                                                                                      <  2:1562294/150‑1 (MQ=255)
                                                               ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCGCGACAATAAAGTCGAGAGAGACACGCGGAGTGGAGCGCCCTACCGGGGCAAAGTCTTCGTGACGTaaaagcctc                                                                                      >  2:1713515/1‑145 (MQ=255)
                                                                cTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatca                                                                                     >  2:1431518/1‑150 (MQ=255)
                                                                  cccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                   >  1:1516419/1‑150 (MQ=255)
                                                                   ccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAACCATCATTa                                                                                  >  1:307350/1‑150 (MQ=255)
                                                                    cGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGCGCGCACTACCGTGGCAAAGTCTTCCTGACGTAACAACATCATTAc                                                                                 >  2:1828498/1‑150 (MQ=255)
                                                                    cGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAGCATCATTAc                                                                                 >  2:1821397/1‑150 (MQ=255)
                                                                         caACCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGACAAAGTCTTCCGGACGGAAAAACAGCATTACCCCCg                                                                            >  2:209706/1‑150 (MQ=255)
                                                                          aaaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAATCATCATTACCCCCGa                                                                           >  2:371130/1‑150 (MQ=255)
                                                                           aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGaa                                                                          >  1:321397/1‑150 (MQ=255)
                                                                             ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAg                                                                        >  2:1361310/1‑150 (MQ=255)
                                                                             ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGACGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTTCCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCACGAAAg                                                                        >  2:1451391/1‑150 (MQ=255)
                                                                               aGTAAACCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGAGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                      >  2:543083/1‑150 (MQ=255)
                                                                                gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCgg                                                                     <  2:1055746/150‑1 (MQ=255)
                                                                                   aCCCTGCGCCGGGCGGTTTGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                                  <  2:820517/150‑1 (MQ=255)
                                                                                    cccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAGACATCATTACCCCCGAAAGCGGTCtt                                                                 >  1:1441214/1‑150 (MQ=255)
                                                                                    cccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCtt                                                                 <  1:778616/150‑1 (MQ=255)
                                                                                     ccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGGGACAAGCGGAGTGGAGCGCCCTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTg                                                                >  1:1736687/1‑150 (MQ=255)
                                                                                       tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                              <  2:1835267/150‑1 (MQ=255)
                                                                                        gcgcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAtt                                                             <  1:1459780/150‑1 (MQ=255)
                                                                                         cgcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGATAGCGGTCGGGATtc                                                            >  2:1639527/1‑150 (MQ=255)
                                                                                             gggCGGGTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCaa                                                        <  1:950070/150‑1 (MQ=255)
                                                                                              ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAGAAACATCATTACCCCCGAAAGCGGTCTTGATTCGCact                                                       >  2:1740299/1‑148 (MQ=255)
                                                                                              ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                       <  1:1531493/150‑1 (MQ=255)
                                                                                              ggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAg                                                       <  1:1711867/150‑1 (MQ=255)
                                                                                                gggttggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                     <  2:991898/149‑1 (MQ=255)
                                                                                                cGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGa                                                     >  2:938200/1‑150 (MQ=255)
                                                                                                      gttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCCCt                                               >  1:528298/1‑150 (MQ=255)
                                                                                                          ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTa                                           >  1:1481765/1‑150 (MQ=255)
                                                                                                          ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGACTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGAAAAAAAATCATTACCCCCGAAAGAGGTCTTGATTCTCAACGCACCACTGGTa                                           >  1:1592475/1‑150 (MQ=255)
                                                                                                              gCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTg                                       >  1:603023/1‑150 (MQ=255)
                                                                                                                cAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGca                                     <  1:1180906/150‑2 (MQ=255)
                                                                                                                 aaGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTg                                    <  1:1301834/150‑1 (MQ=255)
                                                                                                                   gCAGAAAGTCGCCGCGTGTGTTCTCGACAATAAATTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCTTTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  <  1:1187190/150‑1 (MQ=255)
                                                                                                                   gCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  <  1:1066968/150‑1 (MQ=255)
                                                                                                                      gAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAACTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAg                               >  2:1126899/1‑150 (MQ=255)
                                                                                                                            cgccgcGACTGATCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGc                         <  2:1284861/150‑1 (MQ=255)
                                                                                                                             gcagcgACTGTTCTCGACAATAAAGACGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGGCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                        <  1:65943/147‑1 (MQ=255)
                                                                                                                             cccgcGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                        <  2:1270809/149‑1 (MQ=255)
                                                                                                                               cgcgACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTc                      >  1:1751449/1‑150 (MQ=255)
                                                                                                                                   gtctctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt                  >  2:1873886/6‑150 (MQ=255)
                                                                                                                                   aCTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt                  <  1:949719/150‑1 (MQ=255)
                                                                                                                                    cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTg                 <  2:8322/150‑1 (MQ=255)
                                                                                                                                      tctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               >  1:509440/3‑150 (MQ=255)
                                                                                                                                      gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               <  2:1663087/150‑1 (MQ=255)
                                                                                                                                       ttCTAGACAATAATGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgaga              <  1:1584965/150‑1 (MQ=255)
                                                                                                                                         ctcGACAATAAAGTCGAGAGAGACAAGCGGAGTGGATCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACACCCGAAAGCGGACTTGATTCTCAAGGAACCACTGGTAAGTGCTGGAAAGCCCCGGTTCCAGTGAGCtt            >  2:1351501/1‑150 (MQ=255)
                                                                                                                                           cGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTc          >  2:1268284/1‑150 (MQ=255)
                                                                                                                                           cGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATACTCAAAGAACCACTGGTAAGTGCTGGCTAGCCGCGCTTCCAGTGAGAttta          >  2:466667/1‑149 (MQ=255)
                                                                                                                                             ccAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAACGCGGTCTTGATTCTCAAGGAACACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg       <  2:928188/149‑1 (MQ=255)
                                                                                                                                              cAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg       <  1:214666/150‑1 (MQ=255)
                                                                                                                                              aaaTAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg       <  1:430214/149‑1 (MQ=255)
                                                                                                                                                aTAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGAt     <  2:461202/150‑1 (MQ=255)
                                                                                                                                                  aaaGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATAc   >  2:1167597/1‑150 (MQ=255)
                                                                                                                                                   aaGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGCTCTTGAATCTCAAGGAACCACTGGTAAGTGCTGGCATGCCCCGCTTCCAGTGAGAGTTCGTGATAcc  >  2:1709276/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126011‑2126307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: