Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,216,157 A→C 5.9% V107G (GTT→GGT)  yehW ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,1570AC 5.9% 330.0 / 2.8 118V107G (GTT→GGT) yehWputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (51/57);  new base C (5/4);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCC  >  NC_000913/2216010‑2216306
                                                                                                                                                   |                                                                                                                                                     
ccACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCCCCGGACCg                                                                                                                                                     >  2:212564/1‑150 (MQ=255)
ccACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACg                                                                                                                                                     >  1:1047430/1‑150 (MQ=255)
ccACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACg                                                                                                                                                     >  2:586859/1‑150 (MQ=255)
  aCGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGCTCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGCTCCCCGGACCGGc                                                                                                                                                   >  2:1698675/1‑150 (MQ=255)
      cTCATTCCCATCCCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACTCCGTAAAGGATCAACGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATc                                                                                                                                               <  2:299013/150‑1 (MQ=255)
      cTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATc                                                                                                                                               <  1:223784/150‑1 (MQ=255)
        cATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGc                                                                                                                                             <  1:1198138/150‑1 (MQ=255)
         aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGcc                                                                                                                                            <  1:828486/150‑1 (MQ=255)
         aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGcc                                                                                                                                            <  2:1762300/150‑1 (MQ=255)
         aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGcc                                                                                                                                            <  2:801039/150‑1 (MQ=255)
          ttCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCa                                                                                                                                           <  1:1066737/150‑1 (MQ=255)
           tCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCGCCGTTCCGGTGATCggctt                                                                                                                                          <  1:1085294/150‑5 (MQ=255)
            cccATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCGCCGTTCCGGTGATCggcttt                                                                                                                                         <  2:1603770/150‑6 (MQ=255)
            cccATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGc                                                                                                                                         >  2:1745336/1‑150 (MQ=255)
            cccATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGc                                                                                                                                         >  2:627033/1‑150 (MQ=255)
            cccATACCTTTAGCAACTTCTGTCACGCTGGCAGGAAGCGCTCCCAGCCCGTCAAGTGGCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGCTGCAGACCATAGCCGATCACCGGAACGGCGATCGCCAGc                                                                                                                                         <  2:1164772/150‑1 (MQ=255)
             ccATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCCGCCCGGCAAGTGTCGCCTGCACGACGGGCAGCACACCGAAAAGGATCAACGCGCTAATCGCTGGTAGCAGCCCACAGCCGAACACCGGAACGGCGCTCGCCAGCa                                                                                                                                        >  1:85909/1‑150 (MQ=255)
             ccATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCa                                                                                                                                        <  2:249955/150‑1 (MQ=255)
                tACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCAGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTg                                                                                                                                     <  2:1795764/150‑1 (MQ=255)
                 aCCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCAGTGc                                                                                                                                    >  2:263088/1‑150 (MQ=255)
                 aCCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGc                                                                                                                                    <  2:675947/150‑1 (MQ=255)
                 aCCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGc                                                                                                                                    <  1:1738131/150‑1 (MQ=255)
                  ccTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGCCCAAAGCCGATCACCGGAACGGCGATCGCCAGCCCTGCg                                                                                                                                   >  2:510918/1‑150 (MQ=255)
                     ttCGCAACTTCTGTCACGCTGTCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGAGCGCCAGCACTGCGACg                                                                                                                                <  1:284308/150‑1 (MQ=255)
                       cGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACggg                                                                                                                              >  1:522366/1‑150 (MQ=255)
                       cGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACggg                                                                                                                              >  1:1815844/1‑150 (MQ=255)
                        gCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGc                                                                                                                             <  2:91337/150‑1 (MQ=255)
                            cTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGaa                                                                                                                         >  1:1046883/1‑150 (MQ=255)
                            cTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGGTGCAGACCAACGCCGATCACCGGACCGGCGATCGCCAGCACTGCGACGGGCGGaa                                                                                                                         >  1:106241/1‑150 (MQ=255)
                               cTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAg                                                                                                                      <  1:1269519/150‑1 (MQ=255)
                                 gTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTc                                                                                                                    <  1:67881/150‑1 (MQ=255)
                                   cACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCGTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTg                                                                                                                  <  1:1599071/150‑1 (MQ=255)
                                   cACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTg                                                                                                                  <  1:587008/150‑1 (MQ=255)
                                     cGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTc                                                                                                                >  1:1575366/1‑150 (MQ=255)
                                      gCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTcc                                                                                                               <  2:605062/150‑1 (MQ=255)
                                            aTCAATCGCTCCCAGCCCGGTAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcggc                                                                                                         <  2:568725/150‑1 (MQ=255)
                                            aTCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcggc                                                                                                         >  1:427821/1‑150 (MQ=255)
                                             tCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcggcg                                                                                                        <  1:460091/150‑1 (MQ=255)
                                              cAATCGCTCCCAGTCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcggcgg                                                                                                       >  1:591103/1‑150 (MQ=255)
                                              cAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcggcgg                                                                                                       >  2:521685/1‑150 (MQ=255)
                                               aaTCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCTAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACggcggc                                                                                                      >  2:1436437/1‑150 (MQ=255)
                                                aTCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCa                                                                                                     <  1:1427407/150‑1 (MQ=255)
                                                 tCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCaa                                                                                                    <  1:547543/150‑1 (MQ=255)
                                                   gCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCACCGGCGGCAATa                                                                                                  >  1:1532602/1‑150 (MQ=255)
                                                     tCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGt                                                                                                >  1:730610/1‑150 (MQ=255)
                                                      cccAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGCTCACCGGACCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCACCGGCGGCAATAGtt                                                                                               >  2:1762114/1‑150 (MQ=255)
                                                      cccAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCCGCACTGCGACGGGCGGAAAAGGCTGTCCAACGGCGGCAATAggt                                                                                               >  2:770735/1‑148 (MQ=255)
                                                        cAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTc                                                                                             >  2:225963/1‑150 (MQ=255)
                                                                  aGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTggg                                                                                   <  2:333097/150‑1 (MQ=255)
                                                                      tCGCCTGCAGGACGGGCAGCTCACAGTACAGGATCAAGGCGATAATCGCTGGTGGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGaa                                                                               <  2:1217667/150‑1 (MQ=255)
                                                                       cGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGGAATAGTTTCCACCAGTGGGCGaaa                                                                              >  2:74537/1‑150 (MQ=255)
                                                                           tGCAGGACGGGCATCACACAGTAAAGGATCAAGGCGATAATCGCTGGTTGTAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCCCCAGTGGGCGAAAttac                                                                          >  2:806376/1‑148 (MQ=255)
                                                                           ggCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTcc                                                                          <  2:681278/149‑1 (MQ=255)
                                                                            gCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCcg                                                                         >  2:1110627/1‑150 (MQ=255)
                                                                               ggACGGGCAGCACACCGTAAAGGCTCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCCATCGCCAGCACTGCGACGGGCGGAAAAGTCTCTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCgcgc                                                                      >  1:699034/1‑150 (MQ=255)
                                                                                gACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGcc                                                                     <  1:1712587/150‑1 (MQ=255)
                                                                                aaCGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTCCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCACCCAGTGGGCGAAATTCCGCGcc                                                                     >  1:749432/2‑150 (MQ=255)
                                                                                   gggCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCCCTGCGACGGGCGGAAAAGTCTGTCCACCGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGccccc                                                                  >  2:952994/1‑149 (MQ=255)
                                                                                    ggCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCCGACCAAAGCCGATCACCGGACCGGCGCTCGCCCGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCTAAATTCCGCGCCGCAc                                                                 >  1:315211/1‑150 (MQ=255)
                                                                                     gcagcaCACCGTAAAGGATCAAGGCGAAAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACg                                                                <  2:794745/150‑1 (MQ=255)
                                                                                        gcaCACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGc                                                             <  2:1002651/150‑1 (MQ=255)
                                                                                           cacCGTACAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACTGGAACGGCGATCGCCAGCACTGCGTCGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAg                                                          <  2:738205/150‑1 (MQ=255)
                                                                                           cacCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAg                                                          <  2:1406461/150‑1 (MQ=255)
                                                                                           cacCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAg                                                          <  2:1109632/150‑1 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGGCCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        <  2:49939/150‑1 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGCCCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        >  2:262100/1‑150 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        <  1:1342808/150‑1 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        >  1:1041266/1‑150 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        <  1:1488565/150‑1 (MQ=255)
                                                                                             ccGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTg                                                        <  2:427821/150‑1 (MQ=255)
                                                                                               gTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGAc                                                      >  2:1521923/1‑150 (MQ=255)
                                                                                                tAACGGATCAAGGCGATAATCGCTGGTGGCAGCCCAAAGCCGAACACCGGAACGGCGATCGCAAGCACTGCGACGGGCGTAAAAGTCCTTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCTCGGGCGAGTGACa                                                     <  1:1141147/150‑1 (MQ=255)
                                                                                                tAAAGGATCACGGCGATAATCGCTGGTTGCAGCCCAAAGCCGATCACCGGACCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACa                                                     <  1:263088/150‑1 (MQ=255)
                                                                                                 aaaGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAg                                                    <  2:591103/150‑1 (MQ=255)
                                                                                                   aGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGGTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCa                                                  >  2:1578342/1‑150 (MQ=255)
                                                                                                      aTCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAAtt                                               >  1:304567/1‑150 (MQ=255)
                                                                                                          aGGCGATAATCGCTGGTTGCAGCCCAAAGCCGATCACCGGAACGGCGATCGCCACCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGACCCACGGGCGAGTGACAGCAATTCCgg                                           >  2:1200404/1‑150 (MQ=255)
                                                                                                           ggCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGc                                          <  2:1334989/150‑1 (MQ=255)
                                                                                                            gCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCa                                         <  1:452059/150‑1 (MQ=255)
                                                                                                             cGATAATCGCTGGTTGCAGACCAAAGCCGATCACCAGAACGGCGATCGCCAGCCCTGCGACGGGCGGATAAGTCTGTCCAACGGCGGCAATAGTTTCCCCCAGTGGGCGCGATTCCGCGCCCCCCGGGCGCGTGACAGCAATTCCGGCAc                                        >  1:314045/1‑150 (MQ=255)
                                                                                                              gATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAcc                                       <  1:1745336/150‑1 (MQ=255)
                                                                                                              cacaaTCGCTGGTAGCAGAGCATAGCCGATCAACGGAACTGCGATCGCCAGCACTGCGACGGGCGGAAAAGGCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAcc                                       <  2:102904/147‑1 (MQ=255)
                                                                                                                 aaTCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACCGCAATTCCGGCACCAGt                                    >  1:673917/1‑150 (MQ=255)
                                                                                                                 aaTCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGt                                    <  1:40623/150‑1 (MQ=255)
                                                                                                                    cGCTGGTTGCAGCCCAAAGCCGATCACCGGATCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATACCGCGCCCCCCGGGCGAGTGCCAGCAAATCCGGCACCAGTccc                                 >  2:313764/1‑150 (MQ=255)
                                                                                                                     gCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCa                                >  1:950694/1‑150 (MQ=255)
                                                                                                                        ggTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATg                             >  2:432681/1‑150 (MQ=255)
                                                                                                                           tGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATc                          <  1:1201166/150‑1 (MQ=255)
                                                                                                                           ggCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGGCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATc                          <  2:1443528/149‑1 (MQ=255)
                                                                                                                               gACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCg                      >  2:628963/1‑150 (MQ=255)
                                                                                                                                 ccAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGca                    >  1:446144/1‑150 (MQ=255)
                                                                                                                                    aaGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGcaaac                 <  2:1815844/150‑1 (MQ=255)
                                                                                                                                    aaGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGcaaac                 <  2:522366/150‑1 (MQ=255)
                                                                                                                                         gATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGCCAGCAATTCCGGCCCCAGTGCCAATGATCACCGCAAACAAACt            >  2:1173569/1‑150 (MQ=255)
                                                                                                                                            cACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTGCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGa         <  1:1195370/150‑1 (MQ=255)
                                                                                                                                              ccGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGaaa       >  1:1463453/1‑150 (MQ=255)
                                                                                                                                              ccGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGaaa       >  1:1326510/1‑150 (MQ=255)
                                                                                                                                               cGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAAt      <  1:627033/150‑1 (MQ=255)
                                                                                                                                                  aaCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTcc   <  2:1300210/150‑1 (MQ=255)
                                                                                                                                                   aCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTccc  <  1:160467/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACGGCGGCAATAGTTTCCACCAGTGGGCGAAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCC  >  NC_000913/2216010‑2216306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑