Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,315 C→G 5.9% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3150CG 5.9% 398.1 / 12.8 132intergenic (+28/+75)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (57/67);  new base G (5/3);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

Only 100 of 135 total aligned reads displayed.

TACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGA  >  NC_000913/2286166‑2286464
                                                                                                                                                     |                                                                                                                                                     
tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                       <  2:1603564/150‑1 (MQ=255)
 aCTTGCAATCGGGGTCGAAAACGGTAGAATTCGCAGCCCCGAGGCGGCACGTAGCGCATCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAACGCCTCACGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACg                                                                                                                                                      <  2:1314976/150‑1 (MQ=255)
    tGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggtt                                                                                                                                                   >  1:902567/1‑150 (MQ=255)
    tGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGGGCGGACCAATAATCCCAAGAAAAAACCAACCCTTACggtt                                                                                                                                                   >  2:272109/1‑150 (MQ=255)
      cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGCGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTCCggttgg                                                                                                                                                 >  1:1294353/1‑150 (MQ=255)
      cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttgg                                                                                                                                                 <  2:1285237/150‑1 (MQ=255)
         tCGGTGTGGAAAACTGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGAAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttt                                                                                                                                              <  2:1209986/150‑1 (MQ=255)
          cGGTGTGGAAAACGGTAGTATTAGCAGCCTCGAGTTGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttt                                                                                                                                             <  2:1592894/150‑1 (MQ=255)
           ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                            >  1:419038/1‑150 (MQ=255)
             tgtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCAGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                                          <  2:1454324/150‑1 (MQ=255)
             tgtgGAAAACGGTAGTATAAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                                          <  1:1622676/150‑1 (MQ=255)
               tgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                                                                                        >  1:621200/1‑150 (MQ=255)
                ggAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                                                                       <  1:870404/150‑1 (MQ=255)
                 gAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttgg                                                                                                                                      <  1:883337/150‑6 (MQ=255)
                 gAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTatat                                                                                                                                      >  2:777708/1‑150 (MQ=255)
                   aaaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCGTACGGTTGGTTTTTTTATATCt                                                                                                                                    >  2:1777867/1‑150 (MQ=255)
                    aaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGGCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTg                                                                                                                                   <  2:287096/150‑1 (MQ=255)
                       cctagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCAGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGATTTTTTATATCTGCaa                                                                                                                                >  1:1216711/3‑150 (MQ=255)
                        gtagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                                                                               <  1:1263434/150‑1 (MQ=255)
                        gtagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTATCTGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                                                                               >  1:776515/1‑150 (MQ=255)
                         ttttaTTAGCAGCCACGAGTCGGCTCGTAGCGAAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatt                                                                                                                              <  1:335240/147‑1 (MQ=255)
                         tagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatt                                                                                                                              <  2:1190642/150‑1 (MQ=255)
                            taTTAGCAGCCACGAGTCGGCACGTAGCGCATCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaat                                                                                                                           <  1:42913/150‑1 (MQ=255)
                              ttAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                                                                                         <  1:567470/150‑1 (MQ=255)
                              ttAGCAGCCACGAGTCGGAACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGCGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                                                                                         <  1:681750/150‑1 (MQ=255)
                               tAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTTTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                        <  1:1271392/150‑1 (MQ=255)
                               tAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                        >  2:1553602/1‑150 (MQ=255)
                                agcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGa                                                                                                                       <  2:878724/150‑1 (MQ=255)
                                agcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTAGTGCCGACCAAAATTCCCAAGAAAAAACCAACCATTTCGGATGGTTTTTTTATATATGCAATTAATTAGa                                                                                                                       >  2:1243681/1‑150 (MQ=255)
                                 gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                                      >  1:1533971/1‑150 (MQ=255)
                                     ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                                                                                  <  2:1557844/150‑1 (MQ=255)
                                     ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                                                                                  <  1:1734516/150‑1 (MQ=255)
                                        cGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGa                                                                                                               <  2:1831316/150‑1 (MQ=255)
                                             cGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg                                                                                                          <  2:830771/150‑1 (MQ=255)
                                              ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGa                                                                                                         >  2:864109/1‑150 (MQ=255)
                                              ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATATGCAATTAATTCGATAAACAGACCGtca                                                                                                         >  2:792893/1‑148 (MQ=255)
                                              ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGAGTCGGGGGTCGGAGGGTGAAGTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCATACGGATGGTTTGTTTATATCTGCAATAAAGTCGATAAACAGAACGTgc                                                                                                         >  1:265077/1‑149 (MQ=255)
                                                  cGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca                                                                                                     <  2:1831292/150‑1 (MQ=255)
                                                    tAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAAGCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATc                                                                                                   >  1:1318276/1‑150 (MQ=255)
                                                     aGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATca                                                                                                  >  2:1695880/1‑150 (MQ=255)
                                                     aGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATca                                                                                                  <  1:1054313/150‑1 (MQ=255)
                                                          aGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                                                                             <  1:564031/150‑1 (MQ=255)
                                                          aGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                                                                             <  1:556741/150‑1 (MQ=255)
                                                          aGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                                                                             >  2:427895/1‑150 (MQ=255)
                                                           gCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCt                                                                                            >  1:706285/1‑150 (MQ=255)
                                                            ccTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTg                                                                                           <  2:863116/150‑1 (MQ=255)
                                                                gTAGCGCACCGTCATGGGGTGTCGGGGGTCTGAGGTTCAAATCCTCTCGTGCAGACCAAAAATCCCAAGAAATAACCAACACTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTa                                                                                       <  1:1100548/150‑1 (MQ=255)
                                                                   gcgcACCGTCATGGGGTGGCGGGGGTCGGAGGTTAAAATCATATCGTGACGACCAAAAATCCCAAGAAAAAACCAACCCTTACTGTTGGTTTTTTTATATCTTCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAttt                                                                                    <  2:117513/150‑1 (MQ=255)
                                                                    cgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtttt                                                                                   <  1:777708/150‑1 (MQ=255)
                                                                     gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                  <  2:70782/150‑1 (MQ=255)
                                                                     gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                  >  1:409365/1‑150 (MQ=255)
                                                                      cACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATTAACAGACCGTGACACATCACCGCCGGTTTATTTTCt                                                                                 >  2:850519/1‑150 (MQ=255)
                                                                      cACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGATGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                                 <  1:1051908/150‑1 (MQ=255)
                                                                       aCCGTCATGTGGTGTCGGTGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                                                                                <  2:98485/150‑1 (MQ=255)
                                                                       aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                                                                                <  1:657289/150‑1 (MQ=255)
                                                                        ccGTCATGGGGTGTCGGGGGTCGGAGGTTCAGATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACACTTACGGTTGGTTGTTTTATATCGGCAATTAATTCGATAAACAGAACGTGACCCAACACAGCCTGTTAATTTTCTGt                                                                               >  1:772547/1‑150 (MQ=255)
                                                                        ccGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCACCCCTTACGGTTGGTTTTTTTATATCAGCAATTAATTCGATAAACAGACCGTGACACATCACAGCATGTTTATGTTCTGt                                                                               >  1:149136/1‑150 (MQ=255)
                                                                            cATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                           <  2:1332205/150‑1 (MQ=255)
                                                                               ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                                                                        >  1:1252453/1‑150 (MQ=255)
                                                                               ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATACGATAAAAAGACAGTGACACATAACAGCCTGTTTATTTTCTGTTTTCAGa                                                                        >  2:1631468/1‑150 (MQ=255)
                                                                                  gtgtCGGGGGTCGGAGGTTCAAATCCTCTCGGGCCGACCAACACTCCCAAGAAAAAACCAACCCTTACGGGTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGCCCGTGCCACCGCACAGCGTGTTTATTTTCTGTTATCAGAACg                                                                     >  2:1842055/1‑150 (MQ=255)
                                                                                  gggtCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                                                                     <  2:1501784/148‑1 (MQ=255)
                                                                                        ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGa                                                               >  1:1144127/1‑150 (MQ=255)
                                                                                           gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACca                                                            >  2:1567919/1‑150 (MQ=255)
                                                                                                   ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTTTTATCAGAACGTCCAGACCACACCCGcc                                                    <  1:1695880/150‑1 (MQ=255)
                                                                                                   ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGcc                                                    >  1:92123/1‑150 (MQ=255)
                                                                                                   ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGcc                                                    <  2:508235/150‑1 (MQ=255)
                                                                                                   ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGCTTTATTTATATCTGCAATTAATTCGATAAACAGACCGGGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGcc                                                    >  1:96496/1‑150 (MQ=255)
                                                                                                     cAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTg                                                  >  2:837691/1‑150 (MQ=255)
                                                                                                       aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                                <  1:1313504/150‑1 (MQ=255)
                                                                                                         tCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTAAATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTa                                              <  2:349151/150‑1 (MQ=255)
                                                                                                             ctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTcc                                          <  1:1553602/150‑1 (MQ=255)
                                                                                                                     gACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAC‑‑CACCCGCCTGAGTATTCCACGGCGATTc                                >  1:148821/1‑150 (MQ=255)
                                                                                                                          aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                             >  1:1190839/1‑150 (MQ=255)
                                                                                                                            caTCCCAAGAAAAAACCACCCCTTAGGGGTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCGGGCCACACCCGCCTGAGTATTCCACGGCGATTCTAccc                           >  2:100823/2‑150 (MQ=255)
                                                                                                                                ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                       >  1:1874693/1‑150 (MQ=255)
                                                                                                                                ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                       >  1:434972/1‑150 (MQ=255)
                                                                                                                                ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACAGTGACACATAACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCGCACCCGCCTGAGAATTCCACGGAGATTCTACCCCTgc                       >  1:946434/1‑150 (MQ=255)
                                                                                                                                ccAAGAAAAAACCAACCCTTACGGTTGGTATTTTTATATCTGCAATTAAATCGATACACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAACACACCCGCCTGAGTATTCCACGCGGATTATACCCCTgc                       >  1:71203/1‑150 (MQ=255)
                                                                                                                                 cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcg                      >  2:443162/1‑150 (MQ=255)
                                                                                                                                   taaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGcc                    >  1:1036147/3‑150 (MQ=255)
                                                                                                                                    gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa                   >  1:1013462/1‑150 (MQ=255)
                                                                                                                                    gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa                   >  1:1108156/1‑150 (MQ=255)
                                                                                                                                    gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa                   >  1:437878/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATTTCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGACTGTTTATTTTATGTTATCATAACGTCCAGCCCACACCCGCCTGAGTATTCCACGGCGATTCTACCACTGCGCCa                   >  1:994181/2‑150 (MQ=255)
                                                                                                                                      aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt                 >  2:1788747/1‑150 (MQ=255)
                                                                                                                                      aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCATGCGCCAtt                 >  2:98234/1‑150 (MQ=255)
                                                                                                                                       aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTAATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTg                >  2:1007158/1‑150 (MQ=255)
                                                                                                                                       aaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGTGCCATTg                <  1:1181226/150‑1 (MQ=255)
                                                                                                                                        aaaccaaaccTTACGGTTTGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGTACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGc               <  2:1746182/150‑1 (MQ=255)
                                                                                                                                         aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc              <  2:610938/150‑1 (MQ=255)
                                                                                                                                         aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc              <  2:187361/150‑1 (MQ=255)
                                                                                                                                             aaccCTTACGGGTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAg          <  2:1364039/150‑1 (MQ=255)
                                                                                                                                              accCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTTTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGc         <  2:1318276/150‑1 (MQ=255)
                                                                                                                                              accCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGc         <  1:837691/150‑1 (MQ=255)
                                                                                                                                                  ttACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGCCCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgac     >  2:1011312/1‑150 (MQ=255)
                                                                                                                                                   tACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgact    <  2:676678/150‑1 (MQ=255)
                                                                                                                                                   tACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgact    <  1:515944/150‑1 (MQ=255)
                                                                                                                                                    aCGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgactg   <  2:615314/150‑1 (MQ=255)
                                                                                                                                                    aCGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgactg   <  1:986694/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGA  >  NC_000913/2286166‑2286464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: