Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,312,170 G→C 7.7% L194V (CTG→GTG)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,312,1700GC 7.7% 316.6 / 16.9 104L194V (CTG→GTG) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (59/37);  new base C (4/4);  total (63/41)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 104 total aligned reads displayed.

AGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC  >  NC_000913/2312026‑2312316
                                                                                                                                                |                                                                                                                                                  
aGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCAGCAGTACGTTTGGAGCGGGAGATCGCACCACCGATACCGAAACCTTCGAAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGt                                                                                                                                               <  1:557108/150‑1 (MQ=255)
  gTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCa                                                                                                                                             >  1:705196/1‑150 (MQ=255)
   tttCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCAc                                                                                                                                            <  2:1697306/150‑1 (MQ=255)
    ttCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACg                                                                                                                                           >  1:1664158/1‑150 (MQ=255)
      cAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGCTCGCCCCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTATGACGCAGTGCGTCCCgcc                                                                                                                                         >  1:1167660/1‑148 (MQ=255)
      cAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaac                                                                                                                                         <  2:867090/150‑5 (MQ=255)
       aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGCCGCCGTCGCCGTTTTGACGCAGTGCGTCACGAcc                                                                                                                                        >  2:728360/1‑150 (MQ=255)
       aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaacg                                                                                                                                        <  1:387130/150‑6 (MQ=255)
        gCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGATCATCTGTACGTTTGGTGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaacgg                                                                                                                                       <  2:1173477/150‑7 (MQ=255)
        gCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                       >  1:195414/1‑150 (MQ=255)
        gCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                       >  2:1416387/1‑150 (MQ=255)
        gCACGGGCGCCGTTACCGATGAAAGCAGCGGTGTTCTGAGCATCAGTACGGTTGGAGCTGGAGATCGCACCACCGATACCGATACCTTCGTAATCATAAGTGATATAACCGCCGACGCCGTCGCCGTTTTGACGCACTGCGTCaaaacgg                                                                                                                                       <  1:911419/150‑7 (MQ=255)
           cGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgttg                                                                                                                                    <  1:451553/150‑1 (MQ=255)
               cGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttag                                                                                                                                <  2:666543/150‑1 (MQ=255)
               cGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGAACGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttag                                                                                                                                <  1:1598468/150‑1 (MQ=255)
                gCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttagt                                                                                                                               >  1:1516880/1‑150 (MQ=255)
                   gTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGACACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCTACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTAc                                                                                                                            <  1:292065/150‑1 (MQ=255)
                      aCCGATGTAAGCAGCGGTGTTCTGATCATCATTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCTTAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGTCGCAGTGCGTCACGACCGTTGTTAGTTACGcc                                                                                                                         <  2:1481463/150‑1 (MQ=255)
                         gATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACt                                                                                                                      >  1:663629/1‑150 (MQ=255)
                           tGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAg                                                                                                                    <  2:656465/150‑1 (MQ=255)
                                gcagcGGTGTTCTTAGAATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGACGACGTTTTCACGCAGTGCGTCACGACCGTGGTTAGTTTCGGCACTAggtaag                                                                                                               >  2:33551/1‑145 (MQ=255)
                                gcagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAg                                                                                                               >  2:1092397/1‑150 (MQ=255)
                                gcagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGAGGGGACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAg                                                                                                               <  2:283573/150‑1 (MQ=255)
                                     ggTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCtta                                                                                                          >  1:1656914/1‑149 (MQ=255)
                                        gtTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCAcc                                                                                                       >  1:441486/1‑150 (MQ=255)
                                          tCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAg                                                                                                     >  2:1841796/1‑150 (MQ=255)
                                          tCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAg                                                                                                     >  2:1502791/1‑150 (MQ=255)
                                          tCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAg                                                                                                     >  1:3465/1‑150 (MQ=255)
                                           cTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACAAGTAAAGCCTTCACCAGa                                                                                                    <  2:660290/150‑1 (MQ=255)
                                            tGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGAt                                                                                                   >  1:189798/1‑150 (MQ=255)
                                             gAGCATCAGTACGTTTGGTGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATg                                                                                                  <  2:1219659/150‑1 (MQ=255)
                                             gAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCATCACCAGATg                                                                                                  >  2:648663/1‑150 (MQ=255)
                                               ccATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATggg                                                                                                <  2:1440143/149‑1 (MQ=255)
                                                        cGTTTGGAGCTGTAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGttt                                                                                       <  2:529306/150‑1 (MQ=255)
                                                           ttGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTTATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa                                                                                    <  2:1258188/150‑1 (MQ=255)
                                                              gAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                 <  2:1819342/150‑1 (MQ=255)
                                                              gAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGGCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTGCACCAGATGGGTTGCCGTTTTTAccc                                                                                 <  1:1023060/150‑1 (MQ=255)
                                                                gCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATATGTGATAGAACCGCAGCCGCCGTCGCCGTATTGACGCAGTGCGTCACGACCGTTGTTAGTTTCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTg                                                                               >  2:1553860/1‑150 (MQ=255)
                                                                 cTGGAGCTCGCACCACCGATACCGAATCCTTCGTTATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGGTAGGTACGCAACTAGGAAAGCCTTCACCAGATGGGTTGACGTTGTTCCCCTgg                                                                              >  2:7602/1‑150 (MQ=255)
                                                                 cTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTgg                                                                              >  2:1046147/1‑150 (MQ=255)
                                                                  tGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGCCCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGt                                                                             >  1:241266/1‑150 (MQ=255)
                                                                   ggAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGCCGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                            >  2:829329/1‑150 (MQ=255)
                                                                   ggAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                            >  2:439742/1‑150 (MQ=255)
                                                                    gagaTCGCACCACCGATAACGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGGTGCCGTTTTTACCCTGGTAc                                                                           <  1:1824448/150‑1 (MQ=255)
                                                                      gaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCCCCAGATGGGTTGCCGTTTTTACCCTGGTACTg                                                                         >  2:547366/1‑150 (MQ=255)
                                                                      gaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTg                                                                         <  2:490645/150‑1 (MQ=255)
                                                                      gaTCGAATCATCGTTATCGAAACCTTAGTAATCATAAGTGTTATAACCGCGGACTCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACGCTGGTACTg                                                                         <  2:314920/150‑1 (MQ=255)
                                                                        tCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGACACGACCGTTGTTAGATACGCCACTAGTAACGCCTTCACCAGATGGGTTGCAGGTGTTACCCTGGTACTGaa                                                                       >  2:1015741/1‑150 (MQ=255)
                                                                         cGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCCCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAAc                                                                      >  1:978642/1‑150 (MQ=255)
                                                                         cGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAAc                                                                      >  2:151601/1‑150 (MQ=255)
                                                                         cGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAAc                                                                      >  1:1716012/1‑150 (MQ=255)
                                                                         cGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGGTGCCGTTTTTACCCTGGTACTGacc                                                                      >  2:939113/1‑148 (MQ=255)
                                                                          gCACCACCGATACCGAAACCTTCGTAATCATATGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTCCCCTGGTACTGAACa                                                                     >  1:33892/1‑150 (MQ=255)
                                                                          gCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGCCCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACa                                                                     >  1:893348/1‑150 (MQ=255)
                                                                          gCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAGCCTGGTACTGATCa                                                                     >  2:337301/1‑150 (MQ=255)
                                                                            accaccGATACCGCAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTTCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGc                                                                   >  2:1848578/1‑150 (MQ=255)
                                                                              caccGATCCCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGCCCGTTGTTAGTTACGCCACTAGTAAAGCCGTCCCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaa                                                                 >  2:917537/1‑150 (MQ=255)
                                                                                ccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                               >  1:1251055/1‑150 (MQ=255)
                                                                                 cGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt                                                              <  1:1739697/150‑1 (MQ=255)
                                                                                  gATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGtt                                                             >  1:417856/1‑150 (MQ=255)
                                                                                   ctACCGAAACCTTCGTAATCATAAGTTATAGAACCGCAGACGCCGTCGCAGTTTTGTCTTAGTTCGTAACTAACGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGttt                                                            <  1:179217/149‑2 (MQ=255)
                                                                                      ccGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                         >  2:5049/1‑150 (MQ=255)
                                                                                      ccGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                         >  2:486781/1‑150 (MQ=255)
                                                                                      ccGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                         >  1:1215140/1‑150 (MQ=255)
                                                                                                 gaactCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg                                              <  1:95010/146‑1 (MQ=255)
                                                                                                 gTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg                                              >  2:369773/1‑150 (MQ=255)
                                                                                                 gTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg                                              >  1:105387/1‑150 (MQ=255)
                                                                                                  tAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGACCAGCAAAGTTCAGGCCGTCAACCAgc                                             >  1:170010/1‑149 (MQ=255)
                                                                                                  tAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa                                             >  1:838254/1‑150 (MQ=255)
                                                                                                   attctaaaGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGGAAAGCCTTCACCAGATGGGTGGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGAc                                            <  1:1166616/144‑1 (MQ=255)
                                                                                                   aaTCATAAGTGATAGAACCGCCGACGCAGTCGCCGTTTTGACGCAGTGCGTAACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGGTGCCGTTTTTACCCTGGTACTGAACAGCATAGTTCAGGCCGTCAACCAGAc                                            <  1:1455357/150‑1 (MQ=255)
                                                                                                    aTCATACGTGATAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGAcc                                           <  1:1582048/150‑1 (MQ=255)
                                                                                                       aTAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACAgaa                                        >  1:1201104/1‑150 (MQ=255)
                                                                                                        tAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaag                                       <  2:818446/150‑1 (MQ=255)
                                                                                                         caGTGATCGACCCTCCGACGCCGTCGCCTTTTTGACGCAGTGCGGCACTACCGTTGTTTGTTACGCCACTAGTAAATCCTTCACCAGATGGGTGGCCGTTTTTACCCTGGTACTGAACAGCACAGTTCAGGCCGTCAACCAGACCgaaga                                      <  2:1167660/149‑1 (MQ=255)
                                                                                                         aaGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaaga                                      <  1:1579959/150‑1 (MQ=255)
                                                                                                          aGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaagaa                                     >  2:1855520/1‑150 (MQ=255)
                                                                                                          aGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaagaa                                     <  2:830052/150‑1 (MQ=255)
                                                                                                           ttGATAGAACCTCCGACGCCGTCGACGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                    <  2:881296/149‑1 (MQ=255)
                                                                                                           gTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                    >  2:1433950/1‑150 (MQ=255)
                                                                                                           gTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGaagaag                                    <  2:1505825/150‑1 (MQ=255)
                                                                                                                    ccgccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTTGTTACGCCCCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                           >  1:887261/1‑150 (MQ=255)
                                                                                                                    ccgacgACGCCGTCGCCGGTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCAACAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa                           <  1:1127766/150‑1 (MQ=255)
                                                                                                                        cgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGt                       >  2:1543023/1‑150 (MQ=255)
                                                                                                                              cgtcgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCCGACCGAAGAAGTCAGTGTTACGGTAGGTcg                 >  2:1334670/1‑150 (MQ=255)
                                                                                                                                  gccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCCCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa             >  1:977038/1‑150 (MQ=255)
                                                                                                                                   ccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg            >  2:843673/1‑150 (MQ=255)
                                                                                                                                   ccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg            >  1:722073/1‑150 (MQ=255)
                                                                                                                                    ggtcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACCGCAAGGTTCAGGCCGTCGTCCAGACCGAAGCAGTCAGTGTTACGGTAGGTCGCGAAGc           >  1:123446/6‑150 (MQ=255)
                                                                                                                                    cgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc           >  1:953200/1‑150 (MQ=255)
                                                                                                                                     gtcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc          >  1:1033957/5‑150 (MQ=255)
                                                                                                                                     gtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGATGAAGTCAGTGTTACGGTAGGTCGCGAAGcc          >  2:137413/1‑150 (MQ=255)
                                                                                                                                     gtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc          <  1:1841796/150‑1 (MQ=255)
                                                                                                                                      ttttGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTAGTGAACAGCAAAGTTCAGGCCGTCATCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg         <  1:151601/150‑1 (MQ=255)
                                                                                                                                      ttttGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg         >  2:258244/1‑150 (MQ=255)
                                                                                                                                      tcgtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCg         >  1:1583340/4‑150 (MQ=255)
                                                                                                                                        gtGACGCACTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGtt       >  1:1281383/2‑150 (MQ=255)
                                                                                                                                         tGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGGTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGCCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa      >  2:641639/1‑150 (MQ=255)
                                                                                                                                            cGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa   <  2:1664158/150‑1 (MQ=255)
                                                                                                                                             gCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc  >  1:812224/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAC  >  NC_000913/2312026‑2312316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑