Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,898 A→C 7.2% Q113P (CAG→CCG) ‡ alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,8980AC 7.2% 382.0 / 11.0 133Q113P (CAG→CCG) ‡alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base A (66/56);  new base C (8/3);  total (74/59)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTC  >  NC_000913/2407750‑2408044
                                                                                                                                                    |                                                                                                                                                   
aCCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTATAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCATTGGTGTATCGGCGCTTATCGTTCAGGCAATGCAg                                                                                                                                                    >  1:239321/1‑150 (MQ=255)
      cAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTg                                                                                                                                              >  1:226104/1‑150 (MQ=255)
        aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCt                                                                                                                                           <  2:1875904/150‑1 (MQ=255)
        aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGATCAGGCAATGCAGGCATTGCt                                                                                                                                           >  1:1731362/1‑150 (MQ=255)
         gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATGATGAAGCACTACCAGGCTCGGGGCATGCGTGATGTTACCGAGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                          <  1:160387/150‑1 (MQ=255)
            tATTGCGATTCCAAAGGCCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAAc                                                                                                                                       <  1:781506/150‑1 (MQ=255)
            tATTGCGAATCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                       <  1:1144021/150‑1 (MQ=255)
               ttcGATTCCAAAGGTCTTAACTCCGCGCGTAAAGCCATCATGCCGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAAAGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGc                                                                                                                                    <  1:1622203/148‑1 (MQ=255)
               tGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCTGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGc                                                                                                                                    >  1:1277016/1‑150 (MQ=255)
                gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                                   >  1:1112118/1‑150 (MQ=255)
                 cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACGGTGGAAGATATTTTCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                                  >  2:231310/1‑150 (MQ=255)
                 cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                                  <  1:1171042/150‑1 (MQ=255)
                  gATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCggg                                                                                                                                 <  1:168862/150‑1 (MQ=255)
                     tCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAc                                                                                                                              >  1:10182/1‑150 (MQ=255)
                      ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                             >  2:883323/1‑150 (MQ=255)
                       cAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGa                                                                                                                            >  1:623941/1‑150 (MQ=255)
                             tCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGtt                                                                                                                      <  1:254229/150‑1 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                     >  1:709154/1‑150 (MQ=255)
                               tttACTCCGCGCGTAAAGACATCATGCAGGACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                                    <  2:1103283/150‑1 (MQ=255)
                                 tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGATACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                                  <  1:1190387/150‑1 (MQ=255)
                                   cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                <  1:1323177/150‑1 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTCCCAGGCTCGTGGCATGCGTGATGTTACCGGGGAAGCTTTTTAGATCGGCAATGGGGTATCTGAGCTTATCGTTCAGGCATTGCAGGCTTTGCTGAACAGCGGGGCCGCATTGTGGGTTCCTg                                                                                                              >  2:946864/1‑150 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                              >  2:958279/1‑150 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                              >  1:836480/1‑150 (MQ=255)
                                     ccGAGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGATGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                              >  2:193453/1‑150 (MQ=255)
                                      cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATACTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTCCTGc                                                                                                             >  2:1208135/1‑150 (MQ=255)
                                      cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATACTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGc                                                                                                             >  2:443201/1‑150 (MQ=255)
                                        cgagTAAAGCCAGCATGCAGCACTACCAGTCTCGGGGCATGCGGGATGTTACCGTTGAATATATTTACATCGTCAATGTTGTATAGGAGATTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                           <  2:563526/147‑1 (MQ=255)
                                          cgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCa                                                                                                         >  1:327243/1‑150 (MQ=255)
                                          cgTAAAGCCAACATGCAGCACAACCAGGCTCGTGGCATGCGTGATGTTACCGTGCAACATAAAAACATCGGCAATGCTGTATCCCAGCTTACCGTTCACGCAATCCAGGCATAGCTGAACAGCGGGGACGAAATGTTGGTTCCAGCACCa                                                                                                         <  1:990757/150‑1 (MQ=255)
                                           gTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAg                                                                                                        <  2:878699/150‑1 (MQ=255)
                                             aaaGCCAGCATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAt                                                                                                      <  1:320895/150‑1 (MQ=255)
                                               aGCCATAATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAGTa                                                                                                    >  2:1634540/1‑150 (MQ=255)
                                                gCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                                   >  2:1609639/1‑150 (MQ=255)
                                                gCCAGCATGCAGCACTACCAGGCTCGTGTCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCAGTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                                   <  1:291088/150‑1 (MQ=255)
                                                 ccATCATGCAGCAGTACCTGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCATGCACCAGATTaaa                                                                                                  >  2:1814924/1‑148 (MQ=255)
                                                 ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAATTGTTGGTTCCTGCTCCAGATTAcc                                                                                                  >  1:145634/1‑150 (MQ=255)
                                                 ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAcc                                                                                                  >  1:151373/1‑150 (MQ=255)
                                                  catcatGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATAATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTAccc                                                                                                 >  1:1341138/1‑150 (MQ=255)
                                                  catcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAccc                                                                                                 >  1:955184/1‑150 (MQ=255)
                                                   atcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTAAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAAAGTTGGTTCCTGCAGCAGATTAcccc                                                                                                >  2:1753992/1‑149 (MQ=255)
                                                     catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATTGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATAGATGAACAGCGGGAACGAAAAGTTGGTTCCTGAACCAGATTacacaca                                                                                              >  2:1481940/1‑149 (MQ=255)
                                                       tGCAGCACAACCAGGCTCGTGGCAGGCGTGAGGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                            <  1:1143535/150‑1 (MQ=255)
                                                         cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                          >  2:1763770/1‑150 (MQ=255)
                                                          agcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGa                                                                                         >  1:1412466/1‑150 (MQ=255)
                                                            caCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGTTTCCTGCACCAGATTACCCACTCTGGAcc                                                                                       >  2:917436/1‑150 (MQ=255)
                                                              cTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACACACTCTGGACcgc                                                                                     >  2:1205813/1‑150 (MQ=255)
                                                                 ccAGCCGCGTGGCACGCGTGCGGTTACCGTGGCAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCACTGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCGGCACCAGAGTACCCACTCTGGACCgcggc                                                                                  <  2:291065/150‑1 (MQ=255)
                                                                   aGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGTCCGcggcgg                                                                                >  2:1230290/1‑150 (MQ=255)
                                                                        cGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                           <  2:1333754/150‑1 (MQ=255)
                                                                           ggCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCtt                                                                        <  2:1425576/150‑1 (MQ=255)
                                                                             cATGCGTGATGTTACCGTGGAAGATATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCCGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTcc                                                                     <  1:1104427/150‑1 (MQ=255)
                                                                                   tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTGAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                                <  1:1432658/150‑1 (MQ=255)
                                                                                   tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                                <  1:1434696/150‑1 (MQ=255)
                                                                                        ttACCGTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGCGACGAAATGTTGGTTCCTGCACCAGATTACCAACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAg                                                           >  1:435486/1‑150 (MQ=255)
                                                                                        ttACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCCCCCGATTACCCCCTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAg                                                           >  2:137352/1‑150 (MQ=255)
                                                                                          aCCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCg                                                         >  2:215581/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTCCCTCGGCAATGGTGTATCGGCCCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCTACTCTGGACCGCCGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                        <  2:1672341/150‑1 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTTTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCATGCACCAGATTACAAACTCTGGAACGCGGCTGTTTCGCTTTACAGCGGTAAAGCgg                                                        >  1:927584/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                        >  2:1581028/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                        >  2:538599/1‑150 (MQ=255)
                                                                                            cGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGt                                                       >  1:1592427/1‑150 (MQ=255)
                                                                                             ggggAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTg                                                      <  1:1413603/148‑1 (MQ=255)
                                                                                             gTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGGTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTg                                                      <  2:1589947/150‑1 (MQ=255)
                                                                                                gAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAt                                                   >  1:749135/1‑150 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                 <  1:706585/150‑1 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAATGCTGAACAGCGCGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGCTAAAGAGGTGCATTa                                                 >  2:1825869/1‑150 (MQ=255)
                                                                                                    atatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATc                                               <  1:580928/150‑1 (MQ=255)
                                                                                                     tatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                              <  1:1410267/150‑1 (MQ=255)
                                                                                                     tatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                              >  1:1360804/1‑150 (MQ=255)
                                                                                                        ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                           <  2:1855463/150‑1 (MQ=255)
                                                                                                         tACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                          >  2:1285849/1‑150 (MQ=255)
                                                                                                          aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGCAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                         <  2:1065420/150‑1 (MQ=255)
                                                                                                           cATCGGCTATGGTGTATCGGAGGTTTGCGTTCCGGCATTGCAGGCATTGCTGAACAGGGGGGAGGAAATGTTGGGTCCTGCACCAGATTTCCCAGTCTGGACCGCGGCGGTGTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGa                                        <  1:364886/150‑1 (MQ=255)
                                                                                                           cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                                     <  1:482840/137‑5 (MQ=255)
                                                                                                            aTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACTGCGGGGACGAAATGTGGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGAt                                       <  2:1195091/150‑1 (MQ=255)
                                                                                                              cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                                     >  2:472201/1‑150 (MQ=255)
                                                                                                                  caTGGGGGATCGGAGCTTATCGTTCACGCAAAGCAGGCATTGCAGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATcc                                 <  2:327243/149‑1 (MQ=255)
                                                                                                                    tGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCctc                               >  1:1830687/1‑150 (MQ=255)
                                                                                                                       tgtATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGATCAGCGGGGACGAAATGTTGGTTCCTGAACCAGATTACCCAATCTGGACCGCGGCGGTTTCGCTTGCGAGCGGTAAAGCGTTGCAGTATCTTTGCGAGGAATGCTCTGa                            >  1:1429121/1‑150 (MQ=255)
                                                                                                                         tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                          <  1:1060098/150‑1 (MQ=255)
                                                                                                                          aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGATATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCATCTGACTg                         >  1:7078/1‑150 (MQ=255)
                                                                                                                          aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                         >  2:336774/1‑150 (MQ=255)
                                                                                                                           tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGATTgg                        <  1:1687827/150‑1 (MQ=255)
                                                                                                                            cGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGt                       >  2:469748/1‑150 (MQ=255)
                                                                                                                             ggAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGtt                      >  2:518907/1‑150 (MQ=255)
                                                                                                                              gAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGGTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTc                     <  2:1026554/150‑1 (MQ=255)
                                                                                                                                 cTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGGTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg                  <  2:1505937/150‑1 (MQ=255)
                                                                                                                                    gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc               >  2:1809100/4‑150 (MQ=255)
                                                                                                                                     ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAcc              >  1:1698849/3‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGCACAGCGGGGACGAAATGTTGGTTCCGGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCGTGCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAAGCCTCTGCCTGGTTCCCGGACCt             >  1:1450142/1‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTCCCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt             >  1:1679162/1‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt             >  1:961788/1‑150 (MQ=255)
                                                                                                                                        ttCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAATTGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCg           <  2:226104/150‑1 (MQ=255)
                                                                                                                                          tcagCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCga          >  1:1230361/4‑150 (MQ=255)
                                                                                                                                          tcagCAATGCCTGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgat         >  1:620191/4‑150 (MQ=255)
                                                                                                                                          cAGGCAATTCCGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgat         <  2:85052/150‑1 (MQ=255)
                                                                                                                                           cggCCATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGTTGCATTATCTTTGCGATGAATCCTCTGACTGGATCCCGGACCTCgatg        <  2:145634/149‑1 (MQ=255)
                                                                                                                                           aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAGCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCTTTATGTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg        >  2:1243021/1‑150 (MQ=255)
                                                                                                                                           aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg        <  2:151373/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTC  >  NC_000913/2407750‑2408044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: