Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,899 G→T 7.4% Q113H (CAG→CAT) ‡ alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,8990GT 7.4% 361.0 / 15.3 132Q113H (CAG→CAT) ‡alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base G (64/57);  new base T (8/2);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 134 total aligned reads displayed.

ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGC  >  NC_000913/2407750‑2408048
                                                                                                                                                     |                                                                                                                                                      
aCCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTATAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCATTGGTGTATCGGCGCTTATCGTTCAGGCAATGCAg                                                                                                                                                        >  1:239321/1‑150 (MQ=255)
      cAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTg                                                                                                                                                  >  1:226104/1‑150 (MQ=255)
       acgcgTATTGCGAATCCAAAGGTCTTTCCTCCGCGCGTATAGCCATCATCCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                                <  2:120595/146‑1 (MQ=255)
        aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCt                                                                                                                                               <  2:1875904/150‑1 (MQ=255)
        aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGATCAGGCAATGCAGGCATTGCt                                                                                                                                               >  1:1731362/1‑150 (MQ=255)
         gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATGATGAAGCACTACCAGGCTCGGGGCATGCGTGATGTTACCGAGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                              <  1:160387/150‑1 (MQ=255)
            tATTGCGAATCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                           <  1:1144021/150‑1 (MQ=255)
            aaatGCGAGTCCAAAGGTCTCTACGCCGCGCGTAACGCCATCCTGCAGCACTACCAGGCTCGTGGCAGGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                           <  2:907814/147‑1 (MQ=255)
                gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                                       >  2:35800/1‑150 (MQ=255)
                 cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACGGTGGAAGATATTTTCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                                      >  2:231310/1‑150 (MQ=255)
                     tCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAc                                                                                                                                  >  1:10182/1‑150 (MQ=255)
                      ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                                 >  2:883323/1‑150 (MQ=255)
                       cAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGa                                                                                                                                >  1:623941/1‑150 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                         >  1:709154/1‑150 (MQ=255)
                              cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                         <  1:1664568/150‑1 (MQ=255)
                                 tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGATACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                                      <  1:1190387/150‑1 (MQ=255)
                                   cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                    <  2:261294/150‑1 (MQ=255)
                                   cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                    <  1:1323177/150‑1 (MQ=255)
                                   cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGCGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                    >  1:1364389/1‑150 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTCCCAGGCTCGTGGCATGCGTGATGTTACCGGGGAAGCTTTTTAGATCGGCAATGGGGTATCTGAGCTTATCGTTCAGGCATTGCAGGCTTTGCTGAACAGCGGGGCCGCATTGTGGGTTCCTg                                                                                                                  >  2:946864/1‑150 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                  >  2:958279/1‑150 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                  <  2:1489065/150‑1 (MQ=255)
                                     ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                  >  1:836480/1‑150 (MQ=255)
                                     ccGAGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGATGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                  >  2:193453/1‑150 (MQ=255)
                                       gcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTAACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGGGCTTATCGTTCACGCAATGCACTCATTGCTGAACAGCCGGGCCGAAATCTTGGTTCCTGcc                                                                                                                >  1:118622/1‑149 (MQ=255)
                                        cgagTAAAGCCAGCATGCAGCACTACCAGTCTCGGGGCATGCGGGATGTTACCGTTGAATATATTTACATCGTCAATGTTGTATAGGAGATTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                               <  2:563526/147‑1 (MQ=255)
                                          cgTAAAGCCATCATGGAGCACTAGCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGGATCGGAGCGTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGGACCa                                                                                                             <  1:984472/150‑1 (MQ=255)
                                           gTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAg                                                                                                            <  2:878699/150‑1 (MQ=255)
                                            tAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGa                                                                                                           <  1:332325/150‑1 (MQ=255)
                                             aaaGCCAGCATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAt                                                                                                          <  1:320895/150‑1 (MQ=255)
                                               aGCCATAATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAGTa                                                                                                        >  2:1634540/1‑150 (MQ=255)
                                                gCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                                       >  2:1609639/1‑150 (MQ=255)
                                                gCCAGCATGCAGCACTACCAGGCTCGTGTCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCAGTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                                       <  1:291088/150‑1 (MQ=255)
                                                 ccATCATGCAGCAGTACCTGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCATGCACCAGATTaaa                                                                                                      >  2:1814924/1‑148 (MQ=255)
                                                 ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAATTGTTGGTTCCTGCTCCAGATTAcc                                                                                                      >  1:145634/1‑150 (MQ=255)
                                                  catcatGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATAATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTAccc                                                                                                     >  1:1341138/1‑150 (MQ=255)
                                                  catcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAccc                                                                                                     >  1:955184/1‑150 (MQ=255)
                                                   atcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTAAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAAAGTTGGTTCCTGCAGCAGATTAcccc                                                                                                    >  2:1753992/1‑149 (MQ=255)
                                                     catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATTGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATAGATGAACAGCGGGAACGAAAAGTTGGTTCCTGAACCAGATTacacaca                                                                                                  >  2:1481940/1‑149 (MQ=255)
                                                     catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCAca                                                                                                  >  1:1514532/1‑149 (MQ=255)
                                                       tGCAGCACAACCAGGCTCGTGGCAGGCGTGAGGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                                <  1:1143535/150‑1 (MQ=255)
                                                         cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                              >  2:1763770/1‑150 (MQ=255)
                                                         cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACAGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTAACGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                              >  2:1091732/1‑150 (MQ=255)
                                                          agcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGa                                                                                             >  1:1412466/1‑150 (MQ=255)
                                                           gcaCGACCAGGCTCGTGGCATGAGTGATGTAACCGTGGAAGATATTTACAGCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGGGGGGACGAAATGTTGGTGCCTGCACGAGATTACCCACTCTGGAc                                                                                            >  1:1126198/1‑150 (MQ=255)
                                                              cTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACACACTCTGGACcgc                                                                                         >  2:1205813/1‑150 (MQ=255)
                                                                   aGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGTCCGcggcgg                                                                                    >  2:1230290/1‑150 (MQ=255)
                                                                        cGTGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGTCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                               <  1:580206/150‑1 (MQ=255)
                                                                        cGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                               <  2:1333754/150‑1 (MQ=255)
                                                                            gCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCTTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCttt                                                                           <  2:1390241/150‑1 (MQ=255)
                                                                             cATGCGTGATGTTACCGTGGAAGATATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCCGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTcc                                                                         <  1:1104427/150‑1 (MQ=255)
                                                                                   tGATGTTACCGTGGAAGATATTTACTTCGGCAATTGTGTATCGGAGCTTTTCGTTCAGGCAATGCAGTCATTGCTGAATAGCGGGGTCGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCTGCgg                                                                    <  1:1317246/150‑1 (MQ=255)
                                                                                   tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTGAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                                    <  1:1432658/150‑1 (MQ=255)
                                                                                        ttACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCCCCCGATTACCCCCTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAg                                                               >  2:137352/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                            >  2:1581028/1‑150 (MQ=255)
                                                                                           ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                            >  2:538599/1‑150 (MQ=255)
                                                                                            cGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGt                                                           >  1:1592427/1‑150 (MQ=255)
                                                                                                gAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAt                                                       >  1:749135/1‑150 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                     <  1:706585/150‑1 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                     >  1:694814/1‑150 (MQ=255)
                                                                                                  aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAATGCTGAACAGCGCGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGCTAAAGAGGTGCATTa                                                     >  2:1825869/1‑150 (MQ=255)
                                                                                                    atatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATc                                                   <  1:580928/150‑1 (MQ=255)
                                                                                                     tatTTACATCGTCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                                  <  2:1582920/150‑1 (MQ=255)
                                                                                                     tatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                                  <  1:1410267/150‑1 (MQ=255)
                                                                                                      atTTACATCGGCAATGGTCTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCtt                                                 <  1:204100/150‑1 (MQ=255)
                                                                                                        ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                               <  1:1427523/150‑1 (MQ=255)
                                                                                                         tACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                              >  2:205586/1‑150 (MQ=255)
                                                                                                          aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGCAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                             <  2:1065420/150‑1 (MQ=255)
                                                                                                           cATCGGCTATGGTGTATCGGAGGTTTGCGTTCCGGCATTGCAGGCATTGCTGAACAGGGGGGAGGAAATGTTGGGTCCTGCACCAGATTTCCCAGTCTGGACCGCGGCGGTGTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGa                                            <  1:364886/150‑1 (MQ=255)
                                                                                                           cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                                         >  2:482840/1‑133 (MQ=255)
                                                                                                           cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                                         <  1:482840/137‑5 (MQ=255)
                                                                                                            aTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACTGCGGGGACGAAATGTGGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGAt                                           <  2:1195091/150‑1 (MQ=255)
                                                                                                              cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                                         >  2:472201/1‑150 (MQ=255)
                                                                                                              cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                                         <  1:1438264/150‑1 (MQ=255)
                                                                                                                  caTGGGGGATCGGAGCTTATCGTTCACGCAAAGCAGGCATTGCAGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATcc                                     <  2:327243/149‑1 (MQ=255)
                                                                                                                       tgtATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGATCAGCGGGGACGAAATGTTGGTTCCTGAACCAGATTACCCAATCTGGACCGCGGCGGTTTCGCTTGCGAGCGGTAAAGCGTTGCAGTATCTTTGCGAGGAATGCTCTGa                                >  1:1429121/1‑150 (MQ=255)
                                                                                                                         tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                              <  1:1060098/150‑1 (MQ=255)
                                                                                                                          aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                             >  1:890977/1‑150 (MQ=255)
                                                                                                                          aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                             >  2:336774/1‑150 (MQ=255)
                                                                                                                           tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGATTgg                            <  1:1687827/150‑1 (MQ=255)
                                                                                                                            cGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGt                           >  2:469748/1‑150 (MQ=255)
                                                                                                                              gAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGGTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTc                         <  2:1026554/150‑1 (MQ=255)
                                                                                                                                   tATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGa                    <  2:1277016/150‑1 (MQ=255)
                                                                                                                                    gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc                   >  2:1801851/4‑150 (MQ=255)
                                                                                                                                    gctgTTCAGCAATGCCTGCATTGCCTGAAAAGCGGGGACGAAATGTTGGTTACTGCACCAGATTACCCACACTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATTAATACTCTGAATGGTTCCCGGAc                   >  1:733255/4‑150 (MQ=255)
                                                                                                                                     ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAcc                  >  1:1698849/3‑150 (MQ=255)
                                                                                                                                     ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGCTTCCCGGAcc                  >  1:1691236/3‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGCACAGCGGGGACGAAATGTTGGTTCCGGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCGTGCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAAGCCTCTGCCTGGTTCCCGGACCt                 >  1:1450142/1‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTCCCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt                 >  1:1679162/1‑150 (MQ=255)
                                                                                                                                      cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt                 >  1:961788/1‑150 (MQ=255)
                                                                                                                                       gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc                >  1:256955/1‑150 (MQ=255)
                                                                                                                                       gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAAGAAATGATGGTTCCTGAACCCGATTAACCACTCTGGACCGCGGCGGTTTCGCTTTCAAGCGTTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc                >  2:72260/1‑150 (MQ=255)
                                                                                                                                        ttCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAATTGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCg               <  2:226104/150‑1 (MQ=255)
                                                                                                                                          tcagCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCga              >  1:1230361/4‑150 (MQ=255)
                                                                                                                                           cggCCATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGTTGCATTATCTTTGCGATGAATCCTCTGACTGGATCCCGGACCTCgatg            <  2:145634/149‑1 (MQ=255)
                                                                                                                                           cagCAATGCCTGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATAACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGGAACGCGGGCCATTATCTTTGCGATGAATCCTCGGACTGGTTCCCGGACCTCgagg            >  2:1843230/3‑148 (MQ=255)
                                                                                                                                           aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAGCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCTTTATGTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg            >  2:1243021/1‑150 (MQ=255)
                                                                                                                                           aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg            <  2:151373/150‑1 (MQ=255)
                                                                                                                                                 tGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTc      >  2:1666508/1‑150 (MQ=255)
                                                                                                                                                     ggCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCgcgc  <  1:35800/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGC  >  NC_000913/2407750‑2408048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: