Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 176,978 A→T 5.3% intergenic (+24/+23) erpA → / ← yadS iron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913176,9780AT 5.3% 294.1 / 5.0 105intergenic (+24/+23)erpA/yadSiron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (48/49);  new base T (3/3);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

Only 100 of 108 total aligned reads displayed.

TGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTG  >  NC_000913/176830‑177127
                                                                                                                                                    |                                                                                                                                                     
tGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAATGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAc                                                                                                                                                      <  1:172889/150‑1 (MQ=255)
tGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAc                                                                                                                                                      >  1:180250/1‑150 (MQ=255)
tGAGCATGCAATATCTGGTCGGCGGGTCCTTTGATTATACCGAAGGTCTTGAAGGTTCTCGTTTCATCCTGACCAACCCGTACGCGAAAAGCACCTGCGGTTGCTGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAc                                                                                                                                                      <  2:930410/150‑1 (MQ=255)
tGAGACTGCCCTAGCTGGTCGGCGGTTCGGTTGATTATACCTAAGTGCTGGAAGGTACTCGTTTCTTCGTGACCCAGCCGCACTCGAAAAGCACCTGCGTTTGCGGTTCTTCCATTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAc                                                                                                                                                      <  2:930408/150‑1 (MQ=255)
      tGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACACGAACGCGAAAACCACCTGCGGTAGCGGTTCTTCCTTTAGTATCTAATCTGTTGATCATGTGCCGGATAAACTATCCg                                                                                                                                                >  1:1076405/1‑150 (MQ=255)
        cAATATCTGGTCGGCGGTTCCGTTGATTATACCTAAGGTCTGGCAGGTTCTAGTTTCATCGTTTCCAACCCGAACGCGATATGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGc                                                                                                                                              <  1:285910/150‑1 (MQ=255)
         aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             >  2:1027127/1‑150 (MQ=255)
         aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             >  2:1164653/1‑150 (MQ=255)
         aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAACGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGca                                                                                                                                             <  2:1700169/150‑1 (MQ=255)
          atatCTGGTGGGCGGTTCCGTAGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGcac                                                                                                                                            <  2:1711961/150‑1 (MQ=255)
          atatCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGcac                                                                                                                                            <  2:180250/150‑1 (MQ=255)
          atatCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGcac                                                                                                                                            <  1:1575643/150‑1 (MQ=255)
           tatCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGcaca                                                                                                                                           <  1:1505816/150‑1 (MQ=255)
            atCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCCCAt                                                                                                                                          >  2:134665/1‑150 (MQ=255)
             tCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACAtc                                                                                                                                         >  2:27541/1‑150 (MQ=255)
             tCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTCTTCATGTGCCGGATAAACAATCCGGCACAta                                                                                                                                         >  1:681491/1‑149 (MQ=255)
              cTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTTCCGGATAAACTATCCGGCACAtct                                                                                                                                        >  2:665743/1‑150 (MQ=255)
                  tCGGCGGTTCCGTTGATTATACAGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctc                                                                                                                                    <  1:778516/150‑1 (MQ=255)
                         ttCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCaa                                                                                                                             >  2:1755101/1‑150 (MQ=255)
                         ttCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCaa                                                                                                                             >  1:703398/1‑150 (MQ=255)
                          tCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTAGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAAc                                                                                                                            <  1:293681/150‑1 (MQ=255)
                            ctttGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGc                                                                                                                          >  2:1604866/3‑150 (MQ=255)
                             gTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                         >  2:211788/1‑150 (MQ=255)
                             gTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                         >  2:539265/1‑150 (MQ=255)
                             gTTGATTATACCGAAGGTCTGGAAGGGTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                         <  2:518979/150‑1 (MQ=255)
                               tGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCa                                                                                                                       <  2:723578/150‑1 (MQ=255)
                                taTTATACAGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAACAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCAt                                                                                                                      <  1:76153/149‑1 (MQ=255)
                                gATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCAt                                                                                                                      >  1:19131/1‑150 (MQ=255)
                                 aTTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCAtt                                                                                                                     <  1:1164653/150‑1 (MQ=255)
                                  ttATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCTGATAAACTATCCGGCTCATCTTCGCTCTTCAGCGCCCAttg                                                                                                                    >  1:277786/1‑149 (MQ=255)
                                  ttATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCTACGCCCAAtc                                                                                                                    >  1:1186669/1‑150 (MQ=255)
                                  ttATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtc                                                                                                                    >  1:844765/1‑150 (MQ=255)
                                      aCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGATCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTATCAt                                                                                                                >  2:217383/1‑150 (MQ=255)
                                       ccGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATc                                                                                                               >  1:1185334/1‑150 (MQ=255)
                                       ccGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCATCAACGCCCATTctcctc                                                                                                               >  1:692116/1‑150 (MQ=255)
                                          acggTCTGGTAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGGAGCTGCGGATGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCTGATAATCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCac                                                                                                            <  1:301308/148‑2 (MQ=255)
                                              tCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCa                                                                                                        >  1:1302781/1‑150 (MQ=255)
                                                 ggAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAAc                                                                                                     >  2:1583361/1‑150 (MQ=255)
                                                  gacggTTCTCGTTTCATCGTGACCAACCCGACCGCGAAACTCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACg                                                                                                    <  1:256836/147‑1 (MQ=255)
                                                          gcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGc                                                                                            <  1:27541/149‑1 (MQ=255)
                                                            gTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCtt                                                                                          <  2:1275391/150‑1 (MQ=255)
                                                              ttCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTaa                                                                                        <  1:1203469/150‑1 (MQ=255)
                                                              ttCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTaa                                                                                        <  2:1015595/150‑1 (MQ=255)
                                                              ttCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTaa                                                                                        <  1:805368/150‑1 (MQ=255)
                                                               tCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAg                                                                                       <  1:1483817/150‑1 (MQ=255)
                                                                 aTCGTGACCAACCCGAAAGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGAt                                                                                     >  1:1683248/1‑150 (MQ=255)
                                                                   cgggACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGTCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGc                                                                                   <  2:27833/147‑1 (MQ=255)
                                                                    gTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGcc                                                                                  <  1:1755101/150‑1 (MQ=255)
                                                                       aCCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAAc                                                                               <  2:835669/150‑1 (MQ=255)
                                                                        cccccccGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACg                                                                              >  2:96730/5‑150 (MQ=255)
                                                                        ccAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCTCAAACGTCGGTAGCTTAAGATGCCAACg                                                                              <  2:41902/150‑1 (MQ=255)
                                                                        ccAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACg                                                                              <  2:1642842/150‑1 (MQ=255)
                                                                        ccAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACg                                                                              >  2:382831/1‑150 (MQ=255)
                                                                           aCCCGAACGCGAAAATCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCTTGTGCCGGATAAACTATCCGTCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATTCCAACGAAt                                                                           <  2:1376987/150‑1 (MQ=255)
                                                                              cGAACGCGAAAAGCACCTGCGGTTGCGGATCTTCCTTTAGTATCTAATCTGTTCTTCATGTGCCGGATAAACTATCCGGCACAACTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGAGGGTAGCTTAAGATGCCAACGAATAgc                                                                        >  1:1581609/1‑150 (MQ=255)
                                                                                  cgcgAAAAGCACCTGCGGTTGCGGTTCTTCCTGTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATGCGGCACATCTTCTCTCTACAACGCCCAATCTCATCCAGCGCAAACTTCGTTATCTTAAGATGCCAACGAATAgccgcc                                                                    >  2:1197616/1‑150 (MQ=255)
                                                                                    cgAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAg                                                                  >  2:883809/1‑150 (MQ=255)
                                                                                         aGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAAGTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAACCGTCCGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGaa                                                             >  1:120405/1‑150 (MQ=255)
                                                                                         aGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGaa                                                             >  1:535315/1‑150 (MQ=255)
                                                                                           cACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATc                                                           <  1:443222/150‑1 (MQ=255)
                                                                                              cTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAAt                                                        >  2:1483262/1‑150 (MQ=255)
                                                                                                gcggttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAg                                                      <  1:1702260/150‑1 (MQ=255)
                                                                                                  ggttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCg                                                    <  1:709547/150‑1 (MQ=255)
                                                                                                    ttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTc                                                  <  1:335615/150‑1 (MQ=255)
                                                                                                     tgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCa                                                 <  1:1621592/150‑1 (MQ=255)
                                                                                                      gcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATGAGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTATAGATGCAAACGAATAGCAGCCAGCCGAATCAATAGCGTCAc                                                >  2:1203948/1‑150 (MQ=255)
                                                                                                      gcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCAc                                                >  1:1134277/1‑150 (MQ=255)
                                                                                                       cggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACg                                               <  2:1372851/150‑1 (MQ=255)
                                                                                                         gttCTTCCTTTAGTAGCTAATCTGTTGTTCATGTGCCGGAAAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAAAAGCGTCACGAc                                             <  2:1434063/150‑1 (MQ=255)
                                                                                                          ttcttcCTTTAGTATCTAATCTGTTGTGCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGAcc                                            <  1:1022109/150‑1 (MQ=255)
                                                                                                             ttcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATg                                         >  2:645724/1‑150 (MQ=255)
                                                                                                               ccTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGACCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTAACGACCATGcc                                       >  1:13327/1‑150 (MQ=255)
                                                                                                                cTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAACCTATCCGGCACATCTTCTCTCTTCAACGCCCAATCTCATCCAGCGCAAACGTCGGGAGCTTAACATGCCAACGAATAGCCGCCAGCCGAATCAAAAGCGTCCCGCCCATGacc                                      >  2:1157395/1‑150 (MQ=255)
                                                                                                                cTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGccc                                      >  2:874370/1‑150 (MQ=255)
                                                                                                                cTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGccc                                      >  1:1525086/1‑150 (MQ=255)
                                                                                                                      tATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATa                                >  2:1138446/1‑150 (MQ=255)
                                                                                                                         cTAATCTGTTTTTCATGTGCCGGATAAACAATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGTCCATCATACTg                             <  2:1688032/150‑1 (MQ=255)
                                                                                                                         cTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATCCCCATCATACTg                             >  1:510390/1‑150 (MQ=255)
                                                                                                                           aaTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGc                           <  1:59305/150‑1 (MQ=255)
                                                                                                                           aaTCTGGTGGTCATGGGCCGGATAAACAATCCGGCACATCTTCACTCATCAACGCCCATTCTCCTCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGc                           <  2:1185334/150‑1 (MQ=255)
                                                                                                                            aTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCt                          <  2:844765/150‑1 (MQ=255)
                                                                                                                            aTCTGTTGTACATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCAACCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATATCCGCCAGCCGTATCAATAGCGTCACGACCATGCCCATCATACTGGCt                          <  2:277786/150‑1 (MQ=255)
                                                                                                                             tCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTg                         <  1:55975/150‑1 (MQ=255)
                                                                                                                                gttgttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGttt                      >  2:28578/1‑150 (MQ=255)
                                                                                                                                   gttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCTCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCa                   >  1:1116937/1‑150 (MQ=255)
                                                                                                                                   gttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCCCGACCATGCCCATCATACTGGCTGTTTCCa                   >  2:1819328/1‑150 (MQ=255)
                                                                                                                                   gttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCa                   >  2:1561178/1‑150 (MQ=255)
                                                                                                                                      cctgggCCGGAGAAACTATCCGGCACATCATCTCGCTTCAACGCCCCTTCTCCGCCAGCGCAAACGACGGTCTCTTAAGATGCCAACGAATACCCGCCAGCCGAATCAATAGCGTCACGCCCATGCCCATCATCCTGGCTGTGTCCAGTg                <  2:343138/145‑1 (MQ=255)
                                                                                                                                      aaTGGGCCGGATATACTATCCGGCACATCTTCTCTCATCAACGCCCATTCTCAACCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATCGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTg                <  1:327385/149‑1 (MQ=255)
                                                                                                                                       aTGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTATTAGCCCATTATCAACCAGCGCAAACGTCGGTAGCTTAAGATGCCAACTAATAGCCGGCCGCCGCATCAATAGCGTCACGACGATGCCCATCATACTGGCTGTTTCCAGTgg               <  2:81540/150‑1 (MQ=255)
                                                                                                                                        tgtgCCGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCACCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGt              >  1:979352/1‑150 (MQ=255)
                                                                                                                                        tgtgCCGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGATTCCAGAGGt              >  1:1170737/1‑150 (MQ=255)
                                                                                                                                         gtgCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTa             <  2:1633880/150‑1 (MQ=255)
                                                                                                                                         gtgCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTa             <  1:788577/150‑1 (MQ=255)
                                                                                                                                           gCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACg           >  2:79569/1‑150 (MQ=255)
                                                                                                                                           gCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACg           >  1:412280/1‑150 (MQ=255)
                                                                                                                                           gCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACg           >  1:1844095/1‑150 (MQ=255)
                                                                                                                                                  aaaCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAtg    >  1:1610033/1‑150 (MQ=255)
                                                                                                                                                    aCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAtgtg  <  2:1708951/150‑1 (MQ=255)
                                                                                                                                                    aCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAtgtg  <  1:1027127/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTG  >  NC_000913/176830‑177127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: