Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,295 A→T 5.2% intergenic (‑175/+90) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,2950AT 5.2% 471.4 / 11.0 154intergenic (‑175/+90)yfcJ/fabBputative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (74/72);  new base T (4/4);  total (78/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 155 total aligned reads displayed.

GAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440148‑2440444
                                                                                                                                                   |                                                                                                                                                     
gaggagTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATc                                                                                                                                                     <  2:1682125/150‑1 (MQ=255)
     gTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGtt                                                                                                                                                >  2:994390/1‑150 (MQ=255)
        tCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             <  1:1062979/150‑1 (MQ=255)
        tCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             <  2:960849/150‑1 (MQ=255)
        tCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             >  1:51447/1‑150 (MQ=255)
          cccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtt                                                                                                                                           <  1:693017/150‑1 (MQ=255)
           ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCttt                                                                                                                                          <  2:1555503/150‑1 (MQ=255)
           ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGGTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCttt                                                                                                                                          <  2:526669/150‑1 (MQ=255)
           ccGTCTCATCTCTCTGGTTTCAGGGGTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCttt                                                                                                                                          <  2:127414/150‑1 (MQ=255)
           ccGTCTCATCTCTCTGGGTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGTAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCttt                                                                                                                                          <  2:903369/150‑1 (MQ=255)
            cGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttt                                                                                                                                         <  1:1674350/150‑1 (MQ=255)
            cGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGGTGGCAGGATTTAAAGCGAACTTCTTTTCAGAAGGATATCGACAATGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttt                                                                                                                                         <  2:1195247/150‑1 (MQ=255)
             gTCTCATCTCTCTTGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        <  2:1863063/150‑1 (MQ=255)
             gTCTCATCTCTCTGGTTTCAGGTTTTCGGTGCGTTGGCAGGATTTCACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGCGAATTTTGCCTGGAACTGGCGGCGATTGTCAATGATGGGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        <  2:297621/150‑1 (MQ=255)
             gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        <  2:1597273/150‑1 (MQ=255)
               ctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttttca                                                                                                                                      <  1:91681/150‑4 (MQ=255)
               ctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCg                                                                                                                                      <  1:1755446/150‑1 (MQ=255)
                tcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGt                                                                                                                                     >  2:1546279/1‑150 (MQ=255)
                 cATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGtt                                                                                                                                    >  1:1483650/1‑150 (MQ=255)
                   tctctcTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGGTCCCTTTTGCGTTAg                                                                                                                                  <  1:931838/150‑1 (MQ=255)
                       tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACCACGAGGGAAGTTAGCCTGGAACTGGCGGCGATTGTCTATGATCTGAAAAAGGGAACAATCAGGTTACATTTTGCGTTAGTGca                                                                                                                              >  1:285510/1‑149 (MQ=255)
                       tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGcc                                                                                                                              <  2:1848104/150‑1 (MQ=255)
                       tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGcc                                                                                                                              >  1:261408/1‑150 (MQ=255)
                        ctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCg                                                                                                                             >  1:748470/1‑150 (MQ=255)
                         tGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCgg                                                                                                                            >  2:1132294/1‑150 (MQ=255)
                          ggTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGa                                                                                                                           >  2:450347/1‑150 (MQ=255)
                           gTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAg                                                                                                                          >  2:422285/1‑150 (MQ=255)
                            tttCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAgg                                                                                                                         <  1:1565180/150‑1 (MQ=255)
                             ttCAGGGTTACGGTGCGTTGGCAGGATTTCACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGc                                                                                                                        <  1:939234/150‑1 (MQ=255)
                             ttCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGc                                                                                                                        >  1:433390/1‑150 (MQ=255)
                               cAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCg                                                                                                                      <  1:21276/150‑1 (MQ=255)
                               cAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCg                                                                                                                      >  1:174259/1‑150 (MQ=255)
                               cAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGAGTTAGTGCCGGAGGCCg                                                                                                                      >  2:476884/1‑150 (MQ=255)
                                aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGGCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGc                                                                                                                     <  2:23146/150‑1 (MQ=255)
                                        ggTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcgc                                                                                                             >  1:1119659/1‑150 (MQ=255)
                                         gTGCGGTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGcgcg                                                                                                            <  2:1322444/150‑1 (MQ=255)
                                              ttGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACg                                                                                                       <  1:913575/150‑1 (MQ=255)
                                                ggCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     >  2:492351/1‑150 (MQ=255)
                                                 gCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  2:391299/1‑150 (MQ=255)
                                                 gCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    <  1:145384/150‑1 (MQ=255)
                                                       tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCg                                                                                              >  2:1578912/1‑150 (MQ=255)
                                                         tAACGCGTACGTCTTTTCAGAAGGAAATCGCCAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTACCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACTTCGGATGCgcc                                                                                            >  2:668089/1‑148 (MQ=255)
                                                          aaCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACg                                                                                           >  1:776756/1‑150 (MQ=255)
                                                            cgcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         >  1:156877/1‑150 (MQ=255)
                                                                tACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGAGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGgcg                                                                                     <  1:1594457/150‑1 (MQ=255)
                                                                   gTCTTTTCAGTAGGAAATCGACAAAGCGGGAAGGTTGCCTGGAACTGGCGGCGATTGTCAATGATGGGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  <  1:930115/150‑1 (MQ=255)
                                                                     cTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCt                                                                                >  2:597742/1‑150 (MQ=255)
                                                                     cTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCt                                                                                >  1:1292845/1‑150 (MQ=255)
                                                                     cTTTTCACAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGGCAATGATGTGAAAAAGGGAACCATCAGGGGCCCTTTTGCGGTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCt                                                                                <  1:114079/150‑1 (MQ=255)
                                                                          cAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  1:138135/1‑150 (MQ=255)
                                                                          cAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  2:1487061/1‑150 (MQ=255)
                                                                           aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCg                                                                          >  1:42627/1‑150 (MQ=255)
                                                                              cggACATCGACCAAGCGGGAAGTTTGCCTGGAACTGGCGGCGCGTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGAGGCGACGCTGGCGCGTCTACTCCGAcc                                                                       <  1:932065/149‑1 (MQ=255)
                                                                              aGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                       >  2:1564284/1‑150 (MQ=255)
                                                                              aGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                       >  2:1562476/1‑150 (MQ=255)
                                                                              aGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                       >  1:51536/1‑150 (MQ=255)
                                                                                 aaaTCGACAAAGCGGGAAGTTTGCCTGGAAATGGCGGCGATTGTCAATGATGTGAAAAAGGGATCCATCCGGTTCCCTTTTTCGTTAGTGCCGGAGGCCGAATTGGCGCGTAACTTCGGATGCGACGCTGGGGCGTCTCCTCCGACGTAc                                                                    >  2:1641724/1‑150 (MQ=255)
                                                                                  aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACt                                                                   >  2:209082/1‑150 (MQ=255)
                                                                                    ccGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGc                                                                 <  1:807803/149‑1 (MQ=255)
                                                                                     cGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGCACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGAATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTCCTCCGACCGACTGCg                                                                >  2:300156/1‑150 (MQ=255)
                                                                                      gACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTTGCGCGTCTACTCCGCCCTACTGGGa                                                               >  1:1240904/1‑150 (MQ=255)
                                                                                        cAAAGCGGGAAGTTTGCCTGGAACTGGCTGCGATTGTCAATGATGTGAAAAATGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGTAGGCCGCATTGGCGCGTAACGTCTGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             <  1:1147110/150‑1 (MQ=255)
                                                                                        cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             <  2:440629/150‑1 (MQ=255)
                                                                                        cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             >  2:1036131/1‑150 (MQ=255)
                                                                                           aGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                          <  2:748470/150‑1 (MQ=255)
                                                                                             cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATc                                                        >  1:1425559/1‑150 (MQ=255)
                                                                                             cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATc                                                        <  2:51447/150‑1 (MQ=255)
                                                                                                 aaGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGGACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTc                                                    <  1:553290/150‑1 (MQ=255)
                                                                                                  aGTTTTCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGAGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                   <  2:1153109/150‑1 (MQ=255)
                                                                                                       gCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCttt                                              >  1:1492770/1‑149 (MQ=255)
                                                                                                         cTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgcg                                            >  1:742540/1‑150 (MQ=255)
                                                                                                          tGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgcgc                                           <  2:1419957/150‑1 (MQ=255)
                                                                                                           ggAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCa                                          <  2:314202/150‑1 (MQ=255)
                                                                                                                tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTAcc                                     <  1:391299/150‑1 (MQ=255)
                                                                                                                 ggcggcGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCa                                    >  1:421061/1‑150 (MQ=255)
                                                                                                                 agcggcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCa                                    >  1:1648849/2‑150 (MQ=255)
                                                                                                                   cggcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGc                                  >  2:235760/1‑150 (MQ=255)
                                                                                                                           gTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGCCCTACTGCGAATTAATCTTTCAGCATGCGCATTACCAGCGTGGCGtt                          >  2:1490074/1‑150 (MQ=255)
                                                                                                                             cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        <  2:516529/150‑1 (MQ=255)
                                                                                                                             cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  2:593258/1‑150 (MQ=255)
                                                                                                                             cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  2:666418/1‑150 (MQ=255)
                                                                                                                             cAATGATGTGAAAAAGGGAACCATCAGGGTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        <  2:1632775/150‑1 (MQ=255)
                                                                                                                              aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       >  2:1840360/1‑150 (MQ=255)
                                                                                                                               atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTg                      <  1:318829/150‑1 (MQ=255)
                                                                                                                               atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTg                      <  1:1657070/150‑1 (MQ=255)
                                                                                                                                   tgtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTTATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgc                  >  2:1329865/1‑150 (MQ=255)
                                                                                                                                   tgtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgc                  >  1:1817484/1‑150 (MQ=255)
                                                                                                                                    gtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgcc                 >  1:964846/1‑150 (MQ=255)
                                                                                                                                     ggcaaaaGGGAACCATCAGGTGCCCTTTTGCGATAGTGCCGGAGGCCGCATTGGCGCGTACCGTCGGATGCGACGCTGGCGCGGCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                <  2:358402/147‑1 (MQ=255)
                                                                                                                                      taaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGACGGCGCTTTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATAACCAGCGTGGCGTTGGTGCCgccga               <  2:351251/149‑1 (MQ=255)
                                                                                                                                      gcaaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga               >  1:604789/3‑150 (MQ=255)
                                                                                                                                      gcaaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga               >  2:1056444/3‑150 (MQ=255)
                                                                                                                                        aaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaag             >  1:1012921/1‑150 (MQ=255)
                                                                                                                                          aaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagcc           >  1:1595447/1‑150 (MQ=255)
                                                                                                                                           aGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccg          >  1:1108317/1‑150 (MQ=255)
                                                                                                                                              gAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGccgaagccgaag       <  1:450347/150‑1 (MQ=255)
                                                                                                                                              gAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGccgaagccgaag       <  1:1311676/150‑1 (MQ=255)
                                                                                                                                               aaCCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCcgaagccgaagc      <  1:1629321/150‑1 (MQ=255)
                                                                                                                                                 ccATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTg    >  1:118367/1‑150 (MQ=255)
                                                                                                                                                   aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGtt  >  2:771317/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440148‑2440444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: