Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,020 T→A 6.5% R204* (AGA→TGA) ‡ dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0200TA 6.5% 185.9 / 3.3 72R204* (AGA→TGA) ‡dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (15/52);  new base A (3/2);  total (18/54)
Fisher's exact test for biased strand distribution p-value = 9.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

ATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGA  >  NC_000913/2476872‑2477169
                                                                                                                                                    |                                                                                                                                                      
aTCGCCACCCCAATATGAATCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCctgtctg                                                                                                                                                       <  2:494441/150‑1 (MQ=255)
   gCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCAAGCCAgtcagtca                                                                                                                                                    >  1:41482/1‑150 (MQ=255)
        cccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAg                                                                                                                                               <  1:441386/150‑1 (MQ=255)
         ccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGc                                                                                                                                              <  1:1314858/150‑1 (MQ=255)
         ccAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGc                                                                                                                                              <  2:1807729/150‑1 (MQ=255)
            atatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAAc                                                                                                                                           <  1:1539764/150‑1 (MQ=255)
             tatGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATAAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAACg                                                                                                                                          <  1:1734988/150‑1 (MQ=255)
                 aTTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGTCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACa                                                                                                                                      <  1:874952/150‑1 (MQ=255)
                   tCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGt                                                                                                                                    <  1:1332348/150‑1 (MQ=255)
                     aTCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGAATAATGTTGCGCCCAACTATGCCATACATCCGTAACGGAGTCGTTGCTCAAAGCCAGTCTTTCATGGAGCAACCTTAAGTGa                                                                                                                                 >  1:1098918/1‑150 (MQ=255)
                      tCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGac                                                                                                                                <  1:438949/150‑1 (MQ=255)
                        gcgGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATTCCTGGCTGTGATGGAGCAACGTACAGGGaccc                                                                                                                              >  1:1020906/1‑148 (MQ=255)
                        gcgGCAATAACAGCTAAATCAGAACGAGCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGacac                                                                                                                              <  1:1531089/150‑1 (MQ=255)
                           gCAATAACAGCTAAATCAGAACGATCAAAGCCAAATCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAgg                                                                                                                           <  1:1164987/150‑1 (MQ=255)
                             aaTAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGtt                                                                                                                         <  1:177729/150‑1 (MQ=255)
                               tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                       <  1:1611719/150‑1 (MQ=255)
                               tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGTGTGTCATTGAGCAACGTACAGTGACACAGGTTaa                                                                                                                       >  2:1051515/1‑150 (MQ=255)
                                  cAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTa                                                                                                                    <  1:828934/150‑1 (MQ=255)
                                    gCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTGCATGCCTGTCATGCATGGAGCAACTTACAGTGACACAGGTTAATTaac                                                                                                                  >  2:121040/1‑148 (MQ=255)
                                    gCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGGAACGTAAAGTGACACAGGTTAATTaac                                                                                                                  >  2:125712/1‑148 (MQ=255)
                                     cTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCg                                                                                                                 <  2:1055332/150‑1 (MQ=255)
                                       aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCgtg                                                                                                               <  2:757284/150‑1 (MQ=255)
                                       aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTACATGCCTGTCTGTCATGGAGCATCGTACAGTGACACAGGTTAATTTCCgtt                                                                                                               >  2:83029/1‑149 (MQ=255)
                                          tCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTg                                                                                                            <  2:1278171/150‑1 (MQ=255)
                                           cAGAACGAGCAAAGGCAATTCCTGAAGAGGTCGGCTAATTAACCGCTTAATGTTGATCCCGACTATGCCATTGATCTGTACAGGAGTCGTGGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTgg                                                                                                           <  1:171748/150‑1 (MQ=255)
                                           cAGAACGAGCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTAGGCCATTCATCCGTACCGGAGTCGTCGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTgg                                                                                                           <  2:219540/150‑1 (MQ=255)
                                            aGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGt                                                                                                          <  1:1430174/150‑1 (MQ=255)
                                              aaCGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTa                                                                                                        <  1:585989/150‑1 (MQ=255)
                                                cGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTGGCCAGGCCGGGATTTGAAAGAGCAACCTAAAGTGTAACCAGTTTAATAACCGGTTTGGTTaa                                                                                                       >  2:769958/1‑150 (MQ=255)
                                                 gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCTCCCTGCCTGTCTGGGATGGGGCAACGTACAGTTACACACGTTATTTTACGTGTTGGTTaaaa                                                                                                     >  2:1131985/1‑149 (MQ=255)
                                                 gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAg                                                                                                     <  1:1563624/150‑1 (MQ=255)
                                                  aTCACAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGc                                                                                                    <  2:1726606/150‑1 (MQ=255)
                                                  aTCAAAGCCAACTCCCGACGCTGTCGGCAACTTAACCGCATAATGTGGCGCCCAACTATGCCATTCATCCGTACCGGAGTCTTTGCTCCATGCCTGGCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGc                                                                                                    <  2:86337/150‑1 (MQ=255)
                                                    cAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCAt                                                                                                  <  2:1361824/150‑1 (MQ=255)
                                                     aaaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATg                                                                                                 <  1:1586326/150‑1 (MQ=255)
                                                     aaaGCAAATTCCAGAAGATGTCGGCAAATTATCCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATg                                                                                                 <  1:208581/150‑1 (MQ=255)
                                                        gCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCAAGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCt                                                                                              >  1:1316233/1‑150 (MQ=255)
                                                         ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCtt                                                                                             <  2:1830657/150‑1 (MQ=255)
                                                             ttCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAg                                                                                         <  1:818045/150‑1 (MQ=255)
                                                               ccAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATAAGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCg                                                                                       <  1:1812117/150‑1 (MQ=255)
                                                                   aagaTGTAGGCAAATTATCCGCATAATGTTGCGCGCAACAATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTAtt                                                                                   <  2:807563/150‑1 (MQ=255)
                                                                       tGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTGATGGCGCCAAGGACAGTGACACAGGTTTATTTCCGGGTTGGTTAAAGCATGTCGTTGAGCGTATTACgg                                                                               >  2:1318929/1‑150 (MQ=255)
                                                                       tGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATGCCgg                                                                               <  2:1361463/150‑1 (MQ=255)
                                                                        gTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCggg                                                                              <  2:518854/150‑1 (MQ=255)
                                                                         tCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGc                                                                             <  1:1054948/150‑1 (MQ=255)
                                                                             cAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACa                                                                         <  2:1350306/150‑1 (MQ=255)
                                                                                             tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttct                                                         <  2:1267570/150‑1 (MQ=255)
                                                                                             tGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGGCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttct                                                         <  1:125712/150‑1 (MQ=255)
                                                                                             tGTTCCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTAAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttct                                                         <  1:121040/150‑1 (MQ=255)
                                                                                              gTTGCGCCCAACTATGCCAGTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATACCGGGCTACATACTGGCAGGATttctt                                                        <  2:1808256/150‑1 (MQ=255)
                                                                                                    cccAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTGCGGGCTTCAAACTGGCAGGATttcttcctgca                                                  >  1:50075/1‑145 (MQ=255)
                                                                                                             gCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGt                                         <  1:692281/150‑1 (MQ=255)
                                                                                                              ccATTCATCCGTCCCGGAGTCGTTGCTCCATGCCTGGCTGTCATTGAGCAACGTACAGTGACACAGGTTAATTACCGTGGTGGTTAAAGCATGTCGTTGAGCGGATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtg                                        <  2:154644/150‑1 (MQ=255)
                                                                                                                 ttCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                                     <  2:1572671/150‑1 (MQ=255)
                                                                                                                 ttCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                                     <  1:698926/150‑1 (MQ=255)
                                                                                                                     tCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagtg                                 <  1:83029/150‑1 (MQ=255)
                                                                                                                      ccGTACCTGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgagtga                                <  1:1871572/150‑1 (MQ=255)
                                                                                                                          aCCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTg                            <  1:1538023/150‑1 (MQ=255)
                                                                                                                             ggAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                         <  1:1763442/150‑1 (MQ=255)
                                                                                                                              gAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCt                        <  1:1834750/150‑1 (MQ=255)
                                                                                                                               tgTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTg                       <  1:694110/149‑1 (MQ=255)
                                                                                                                                   gTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACgg                   >  1:643300/1‑150 (MQ=255)
                                                                                                                                     tGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcg                 >  2:496833/1‑150 (MQ=255)
                                                                                                                                      gCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGGTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc                <  1:532445/150‑1 (MQ=255)
                                                                                                                                      gCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTAAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcgc                >  2:1120561/1‑150 (MQ=255)
                                                                                                                                       cTCCATGCCTGTCTGTCATGTAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCTTCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca               <  1:1500721/150‑1 (MQ=255)
                                                                                                                                       cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca               <  1:519558/150‑1 (MQ=255)
                                                                                                                                       cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca               >  1:1607435/1‑150 (MQ=255)
                                                                                                                                       cTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca               >  2:1821093/1‑150 (MQ=255)
                                                                                                                                        tCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcat              >  2:1505233/1‑150 (MQ=255)
                                                                                                                                            tGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc          <  1:1051515/150‑1 (MQ=255)
                                                                                                                                            tGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc          <  2:1515454/150‑1 (MQ=255)
                                                                                                                                             gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca         <  2:496225/150‑1 (MQ=255)
                                                                                                                                              ccTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaa        >  2:958312/1‑150 (MQ=255)
                                                                                                                                               ctgtctgtCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaaa       >  1:1740790/1‑150 (MQ=255)
                                                                                                                                                tgtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAg      >  1:912384/1‑150 (MQ=255)
                                                                                                                                                tgtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGGCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAg      <  1:1605669/150‑1 (MQ=255)
                                                                                                                                                 gtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTATCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCAATGAGTAAGTGATTAGTGAGTTGCGCTGTCGGCGCATCATCAAAGt     <  2:670206/150‑1 (MQ=255)
                                                                                                                                                 gtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGt     >  2:184675/1‑150 (MQ=255)
                                                                                                                                                 gtctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGt     <  2:780538/150‑1 (MQ=255)
                                                                                                                                                   ctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAg   >  1:863816/1‑150 (MQ=255)
                                                                                                                                                   ctgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTAGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAg   >  1:1521752/1‑150 (MQ=255)
                                                                                                                                                    tgtcATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGTTTTCTTCATCCTTCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGa  >  1:653227/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATCGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGA  >  NC_000913/2476872‑2477169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: