Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,728 C→A 5.6% intergenic (‑22/‑314) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7280CA 5.6% 348.5 / 8.5 127intergenic (‑22/‑314)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (60/59);  new base A (2/5);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

Only 100 of 129 total aligned reads displayed.

TGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAC  >  NC_000913/2512579‑2512876
                                                                                                                                                     |                                                                                                                                                    
tGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGcc                                                                                                                                                      <  1:846176/150‑1 (MQ=255)
 gAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCt                                                                                                                                                     >  1:1751600/1‑150 (MQ=255)
 gAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCt                                                                                                                                                     >  2:1365562/1‑150 (MQ=255)
      ttGGTCGCAAAGTTACCGGGATCGATAAAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                                                                                                                <  2:833394/150‑1 (MQ=255)
       tGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTa                                                                                                                                               <  2:801050/150‑1 (MQ=255)
         gTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATg                                                                                                                                             <  2:588302/150‑1 (MQ=255)
          tCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGt                                                                                                                                            >  2:1090529/1‑150 (MQ=255)
           cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                           <  1:137975/150‑1 (MQ=255)
           cGCAAAGTTACCGGGATCGATATAACAACTCGCCGCAATGAACGCCGGTCCCATTAATGCGAGACTCATGTTGCGCGGCGCCAGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                           <  2:939817/150‑1 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                         <  2:486849/150‑2 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                         <  2:697500/150‑2 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGACGCAATGAACGCAGGTCCCATTAATTCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTc                                                                                                                                         <  1:534422/150‑1 (MQ=255)
               aaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCAt                                                                                                                                       <  1:380722/150‑1 (MQ=255)
                aGTTAACGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATg                                                                                                                                      <  2:940596/150‑1 (MQ=255)
                  ttACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGttttagagg                                                                                                                                    <  1:519892/150‑7 (MQ=255)
                     ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                 >  1:1134069/1‑150 (MQ=255)
                     ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGGTTCATGCTATg                                                                                                                                 >  1:491185/1‑150 (MQ=255)
                     ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCATCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                 >  2:1737041/1‑150 (MQ=255)
                        ggATCGATATAACCAATCTCCGCAATGAACGCAGGTCCCATAAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCa                                                                                                                              <  1:94710/150‑1 (MQ=255)
                        ggATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCa                                                                                                                              >  1:115144/1‑150 (MQ=255)
                           tCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAAc                                                                                                                           >  1:1864779/1‑150 (MQ=255)
                             gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                         >  1:1411596/1‑150 (MQ=255)
                              atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                        >  2:780198/1‑150 (MQ=255)
                                 taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTCCCTCTAAAACATAGACTTTGCTATGTTTCATGCTATGCCCAACGAGAAt                                                                                                                     >  2:534219/1‑150 (MQ=255)
                                   aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGa                                                                                                                   >  1:1244157/1‑150 (MQ=255)
                                     cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGAtt                                                                                                                 >  1:1075408/1‑150 (MQ=255)
                                     cAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCACCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGACAATGAtt                                                                                                                 >  1:848813/1‑150 (MQ=255)
                                       aTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAGAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTAt                                                                                                               <  1:877746/150‑1 (MQ=255)
                                          gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                            >  1:852946/1‑150 (MQ=255)
                                           ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaaa                                                                                                           >  2:138870/1‑150 (MQ=255)
                                           ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaaa                                                                                                           >  1:515804/1‑150 (MQ=255)
                                            ctcactGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGAGCGCAGCCCGTCCGCTGATACTATCAACTCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAt                                                                                                          <  1:1082903/145‑1 (MQ=255)
                                              cAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTc                                                                                                        >  2:476765/1‑150 (MQ=255)
                                              cAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGACCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGCGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTc                                                                                                        <  2:1330108/150‑1 (MQ=255)
                                               aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                       >  2:1772694/1‑150 (MQ=255)
                                                   aaCGAAGGTCCCATTAATGCGAGCCTCATATTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAGTCAATTa                                                                                                   >  2:518714/1‑150 (MQ=255)
                                                    aCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTaa                                                                                                  >  1:601577/1‑150 (MQ=255)
                                                      gCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                <  1:1707644/150‑1 (MQ=255)
                                                       cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                               <  1:1507588/150‑1 (MQ=255)
                                                            cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                          <  2:781777/150‑1 (MQ=255)
                                                                ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                      <  2:852946/150‑1 (MQ=255)
                                                                    tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                  <  2:791736/150‑1 (MQ=255)
                                                                    tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                  >  2:654419/1‑150 (MQ=255)
                                                                      cGAGCCTCATCTTGTGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtc                                                                                >  2:1095750/1‑150 (MQ=255)
                                                                         gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCCTGCTATGCCAAACGAGAATGATTATCAACTTCATTTAAATGGATTGTGGTGATTTctct                                                                             >  1:934169/1‑150 (MQ=255)
                                                                          ccTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTg                                                                            <  2:572291/150‑1 (MQ=255)
                                                                          ccTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTg                                                                            <  1:138870/150‑1 (MQ=255)
                                                                           cTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCCTCTTGTGCCTCTAAAACATCGCCTTTGCTATGGTTCCTGCTATGCCAAACGAGAATGATTATCAAATTCATTTTAATGGATTGTGGTGATTTCTCTGa                                                                           >  2:278797/1‑150 (MQ=255)
                                                                               tCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGa                                                                       >  2:1321957/1‑150 (MQ=255)
                                                                                cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                      <  1:234546/150‑1 (MQ=255)
                                                                                 ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                     <  2:393535/150‑1 (MQ=255)
                                                                                  tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGATATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                    >  2:1102970/1‑150 (MQ=255)
                                                                                       gccgccGGCCCGCTGCTACTATCACCGCTATTGTTCGTCAATTTGTCCCACAAAAACATCCCCTTTGCTATGTTTCATGCTATCCCAAACCATACTGATAATCAATTTCATTTAAATGCATGGTGGTGATTTCTCTGAAACAcccgtata                                                               <  2:1029760/150‑7 (MQ=255)
                                                                                       gccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAtt                                                               <  1:3257/150‑1 (MQ=255)
                                                                                          gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                            <  2:734912/150‑1 (MQ=255)
                                                                                              gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                        <  2:119335/150‑1 (MQ=255)
                                                                                                        aCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACAAAGCCTTAGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAtt                                              <  2:639167/150‑1 (MQ=255)
                                                                                                          tctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGATAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtga                                            <  2:1276157/150‑1 (MQ=255)
                                                                                                             cAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAATATtgatga                                         >  1:1236511/1‑150 (MQ=255)
                                                                                                              aaCGCGATAGATCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                        <  2:174005/150‑1 (MQ=255)
                                                                                                                cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAATTGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAATATTGATGACtt                                      >  1:1832761/1‑150 (MQ=255)
                                                                                                                cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACtt                                      <  1:499674/150‑1 (MQ=255)
                                                                                                                cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACtt                                      >  1:1791350/1‑150 (MQ=255)
                                                                                                                  cgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAACTGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGa                                    <  1:488718/150‑1 (MQ=255)
                                                                                                                     tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                 <  2:1459732/150‑1 (MQ=255)
                                                                                                                     tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                 <  1:386816/150‑1 (MQ=255)
                                                                                                                      aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAAttt                                >  2:829038/1‑148 (MQ=255)
                                                                                                                      aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATCTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                >  2:694003/1‑150 (MQ=255)
                                                                                                                         tCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttat                             >  2:494627/1‑150 (MQ=255)
                                                                                                                          cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                            >  2:163504/1‑150 (MQ=255)
                                                                                                                           ttCATCTTGTGCCTCTAAAAAATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGTTTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAACGATTGATGACTTGAATgttatgg                           <  2:1360450/149‑1 (MQ=255)
                                                                                                                           gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                           >  1:1104237/1‑150 (MQ=255)
                                                                                                                            tCATCTTGTGCCGCTAAAACTTAGCCATTGCTATGTTTCATGCTATGGCAAACGAGAATGATTATCATATTCATTTAAATGGATTGTGGTGATTTCACTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                          <  2:1193587/150‑1 (MQ=255)
                                                                                                                             cATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggtt                         >  2:960365/1‑150 (MQ=255)
                                                                                                                             cATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggtt                         >  2:1790578/1‑150 (MQ=255)
                                                                                                                              aTCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGATTgttatggtta                        >  1:432128/1‑150 (MQ=255)
                                                                                                                                ctagtgCCTCTAAAACATAGCCGTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttatg                      <  1:91237/147‑1 (MQ=255)
                                                                                                                                 ttGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGt                     >  2:137696/1‑150 (MQ=255)
                                                                                                                                  tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                    >  1:1309510/1‑150 (MQ=255)
                                                                                                                                   gttccTCTTATACATATCATTTTCTATGTTTCATGCTATGCCATACGAGTATGATAATCAAATTCATTTAAAAGGATTGTGGTGATTGCTTTGATAGACCAGGATTATGACTACGAAAATATTGATGAATTGAATGTTATGGTTATGttt                   <  2:1733702/147‑1 (MQ=255)
                                                                                                                                    tgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTa                  >  2:1235733/1‑150 (MQ=255)
                                                                                                                                     gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGTTTGATGACTTGAATGTTATGGTTATGTTTaa                 >  1:965285/1‑150 (MQ=255)
                                                                                                                                       gcatgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg               >  1:1328900/6‑150 (MQ=255)
                                                                                                                                       ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTGATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg               <  2:4645/150‑1 (MQ=255)
                                                                                                                                       ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg               <  2:491185/150‑1 (MQ=255)
                                                                                                                                       ctctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg               >  1:1197086/1‑150 (MQ=255)
                                                                                                                                        tctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt              >  2:1610066/1‑150 (MQ=255)
                                                                                                                                         ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt             >  2:1048917/1‑150 (MQ=255)
                                                                                                                                         ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGGATTATGACTACGAAAAGATTGATGACTTTATTGTTATGGTTATGTTTATTGtt             >  1:1245644/1‑150 (MQ=255)
                                                                                                                                         atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt             >  1:437299/4‑150 (MQ=255)
                                                                                                                                          tAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa            <  2:115144/150‑1 (MQ=255)
                                                                                                                                           aaaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATAATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg           <  2:507743/150‑1 (MQ=255)
                                                                                                                                            aaaCATTGCCTTTGCTATGTTTCATGCTATGACAATCGAGAATGATTATCAAATTCATTTATATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTTAGAATAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc          <  1:935169/150‑1 (MQ=255)
                                                                                                                                             aaCATAGCCTTTGCTATGTTTCATGATATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGAGTACGAGAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca         <  1:534219/150‑1 (MQ=255)
                                                                                                                                                aTAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAt      >  1:427732/1‑150 (MQ=255)
                                                                                                                                                  aGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGCCCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAttt    >  2:312850/1‑150 (MQ=255)
                                                                                                                                                  aGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAttt    <  1:1693796/150‑1 (MQ=255)
                                                                                                                                                   gCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTa   >  1:615622/1‑150 (MQ=255)
                                                                                                                                                   gCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTa   >  2:470878/1‑150 (MQ=255)
                                                                                                                                                    ccTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTaa  >  1:693074/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAC  >  NC_000913/2512579‑2512876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: