Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 184,240 G→T 10.6% intergenic (‑145/+17) yaeH ← / ← yaeI UPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913184,2400GT10.6% 209.2 / 3.4 83intergenic (‑145/+17)yaeH/yaeIUPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base G (47/25);  new base T (2/9);  total (49/34)
Fisher's exact test for biased strand distribution p-value = 6.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCG  >  NC_000913/184092‑184389
                                                                                                                                                    |                                                                                                                                                     
aCAAAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCGTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGt                                                                                                                                                      <  2:315037/150‑1 (MQ=255)
 cATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTg                                                                                                                                                     <  2:754067/150‑1 (MQ=255)
   tAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTTGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGAt                                                                                                                                                   >  2:1268579/1‑150 (MQ=255)
   tAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGAt                                                                                                                                                   >  2:537621/1‑150 (MQ=255)
     gctttACCTCAAAATTAGTCAGTAAATTGTGCTTATTTTATCATTTGGACTGGCCCGACAGTCTCGACTGTGATAGCAGAAAGCATTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATaa                                                                                                                                                 <  1:1152687/147‑1 (MQ=255)
     gCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATaa                                                                                                                                                 <  1:1542897/150‑1 (MQ=255)
      cGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGGGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCAGGGCGTAAAAATCAAAAAATTACATGCTTTATTCTCGTGATaaa                                                                                                                                                >  1:1102695/1‑150 (MQ=255)
           ccTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCa                                                                                                                                           <  1:194406/150‑1 (MQ=255)
            cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAc                                                                                                                                          <  1:9349/150‑1 (MQ=255)
            cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGAGCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAc                                                                                                                                          <  2:280295/150‑1 (MQ=255)
             tCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACg                                                                                                                                         <  1:621907/150‑1 (MQ=255)
             tCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTACCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACg                                                                                                                                         >  2:362380/1‑150 (MQ=255)
              cAAAATGAGTCAGTAAATTTTGCTTATTTTAGCATTTGGCCTTGCCCGCCCGTCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCAATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGa                                                                                                                                        <  2:1702198/150‑1 (MQ=255)
              cAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAAGa                                                                                                                                        >  2:928692/1‑150 (MQ=255)
              cAAAATGAGTCAGGAAATTCGGCTTATCTTAGCACTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAACTCAAAAAATTACCTGCTATATTCTGGTGATAAAATTCACGa                                                                                                                                        <  2:1702193/150‑1 (MQ=255)
                 aaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTCGCCTGGCCCGCCCGGCGCGACTGTGATCGCAGAAAGCCTTTTTCATCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCt                                                                                                                                     <  2:1182592/150‑1 (MQ=255)
                   tGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTac                                                                                                                                   <  1:314873/150‑1 (MQ=255)
                    gAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTaca                                                                                                                                  <  1:1530510/150‑1 (MQ=255)
                    gAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTaca                                                                                                                                  >  2:1006859/1‑150 (MQ=255)
                      gTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGAGATCGCAGAAAGCCTTTTTCTTCAAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGAGATAAAATTCACCATCTACAcc                                                                                                                                >  2:871904/1‑150 (MQ=255)
                       tCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCa                                                                                                                               >  1:988944/1‑150 (MQ=255)
                        cAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATACACGATCTACACCAg                                                                                                                              >  1:1468537/1‑150 (MQ=255)
                        aaGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAg                                                                                                                              <  1:464849/149‑1 (MQ=255)
                             aaTTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTATATCATGGCTTAAAAATCAAAATTTTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCa                                                                                                                         <  2:137944/150‑1 (MQ=255)
                               ttGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAga                                                                                                                       >  1:651043/1‑149 (MQ=255)
                                   gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCg                                                                                                                   >  2:1549969/1‑150 (MQ=255)
                                   gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCg                                                                                                                   >  2:35383/1‑150 (MQ=255)
                                   gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTATTTTGATAAAATTCACGATCTACACCATTTCCAGCATCg                                                                                                                   >  2:292894/1‑150 (MQ=255)
                                    cTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATAAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGt                                                                                                                  <  2:1439761/150‑1 (MQ=255)
                                      tATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTACCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTATGGTGATAAAATTAACGATCTACACCAGTTACAGCATCGTTa                                                                                                                >  2:45755/1‑150 (MQ=255)
                                       aTTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTaa                                                                                                               >  1:523437/1‑149 (MQ=255)
                                        ttttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACt                                                                                                              >  2:1454897/1‑150 (MQ=255)
                                            aGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCTCAGAAAGCCTTTTTCTTCCAGACTTCTATCAAGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATATACACCACTTCCAGCATCGTTACTTCCg                                                                                                          >  2:1227866/1‑150 (MQ=255)
                                                  tggcctggccCGCCCTGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                    <  1:415773/150‑1 (MQ=255)
                                                  tggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                    <  1:1184582/150‑1 (MQ=255)
                                                  tggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                    <  1:1440160/150‑1 (MQ=255)
                                                    gcctggccCGCCCGGCTCTACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                  <  2:1197932/150‑1 (MQ=255)
                                                    gcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGCCGGAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                  >  1:168825/1‑150 (MQ=255)
                                                     cctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGt                                                                                                 >  1:1444132/1‑150 (MQ=255)
                                                        ggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTATTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTATTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCa                                                                                              <  2:508723/150‑1 (MQ=255)
                                                          cccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTAACATCGCGTAAAAATCAAAAAATTACAACCTTAATTCTGTTGAAAAAATTAACGATCTACACCAGTTCCACCATCGTTACTACCGGGCGGCAGATCAGa                                                                                            >  2:585054/1‑150 (MQ=255)
                                                           ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                           >  2:1818147/1‑150 (MQ=255)
                                                              cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                       >  2:683136/1‑150 (MQ=255)
                                                                     cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa                                                                                 >  2:1598723/1‑150 (MQ=255)
                                                                      gACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATAc                                                                                >  2:929238/1‑150 (MQ=255)
                                                                       aCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACTATCTAAACAAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa                                                                               >  1:787090/1‑150 (MQ=255)
                                                                       aCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa                                                                               <  1:569825/150‑1 (MQ=255)
                                                                       aCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTACAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa                                                                               >  1:580227/1‑150 (MQ=255)
                                                                        cTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                              >  1:1624887/1‑150 (MQ=255)
                                                                           tgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACt                                                                           <  1:677097/150‑1 (MQ=255)
                                                                            gATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAACCTg                                                                          >  1:1193315/1‑150 (MQ=255)
                                                                             aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGATCCAGCATCGTTACTTCAGGGCGGCAGTTCAGAAGCAAACCATACAAACTGc                                                                         >  1:911751/1‑150 (MQ=255)
                                                                              tCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATAAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                        <  2:984483/150‑1 (MQ=255)
                                                                                gCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCcca                                                                      >  2:1347475/1‑150 (MQ=255)
                                                                                gCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACATGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAAGCAAACCATACAAACTGcccc                                                                      >  1:994009/1‑149 (MQ=255)
                                                                                   gAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgc                                                                   >  2:709395/1‑150 (MQ=255)
                                                                                   gAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgc                                                                   >  2:1721867/1‑150 (MQ=255)
                                                                                    aaaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTAATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                  <  1:1161083/150‑1 (MQ=255)
                                                                                         cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                             <  2:1102695/150‑1 (MQ=255)
                                                                                         cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                             >  1:1641411/1‑150 (MQ=255)
                                                                                         cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTAACGATCTAAACCAGTTACAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                             >  1:330364/1‑150 (MQ=255)
                                                                                            tttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTATATTTTTTATTGTGTTGATTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtg                                                          <  2:523437/150‑1 (MQ=255)
                                                                                            tttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtg                                                          <  1:1006859/150‑1 (MQ=255)
                                                                                               cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCATTTCAGACGCAAACCATACAAACTTCCCACGCCACGTGTTgtgt                                                       >  2:1145321/1‑150 (MQ=255)
                                                                                               cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCAGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTgtgt                                                       >  2:216213/1‑150 (MQ=255)
                                                                                               attcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTgtgt                                                       <  1:180006/149‑1 (MQ=255)
                                                                                                ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                      >  2:283221/1‑150 (MQ=255)
                                                                                                ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                      >  2:1493542/1‑150 (MQ=255)
                                                                                                   cAGACTTCTATCATGGCGTAAAAATCAAAAAATTCCCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAt                                                   <  1:1390646/150‑1 (MQ=255)
                                                                                                   cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAt                                                   >  1:1232536/1‑150 (MQ=255)
                                                                                                    acaCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACTATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtg                                                  <  1:292894/148‑1 (MQ=255)
                                                                                                    aGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtg                                                  <  2:1106874/150‑1 (MQ=255)
                                                                                                       cTTCTATCATGGCGTAAAAATCAAAAAAATACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCAAGGGTTGTGTAGATgtgt                                               >  2:413470/1‑150 (MQ=255)
                                                                                                            aTCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTCACTTCCGGGCGCCAGTTCAGACGCAAACAATACAAACTGCCCACGCCACGGGTTTTTTAGATGTGTCttta                                          >  2:143173/1‑149 (MQ=255)
                                                                                                               aTGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGcc                                       >  2:150604/1‑150 (MQ=255)
                                                                                                                    gTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAgg                                  <  1:537621/150‑1 (MQ=255)
                                                                                                                         aaTCAAAAAATTACTTGCTTTATTGTTTTGATAAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTGGATGTGTCTTTCGCCAAAGGCAttt                             <  2:1468537/150‑1 (MQ=255)
                                                                                                                           tCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCAAACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTat                           >  1:1439376/1‑149 (MQ=255)
                                                                                                                            cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAAt                          >  2:1474735/1‑150 (MQ=255)
                                                                                                                            cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACAATACAAACTGCCCACGCCACGGGTTGTGAAGATGTGTCTTTCGCCAAAGGCATTTaaa                          >  1:1545071/1‑149 (MQ=255)
                                                                                                                             aaaaaaTAACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCAATCATCGTTACTTCCGTGCGGCAGTTCAGACGCAAACCATACAAACTGCCCAAGCCACGGGTTGTGTAGATGTGTCTTTCTACAAAGGCATTTAATc                         >  2:1562934/1‑150 (MQ=255)
                                                                                                                                                tCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATc      >  2:490580/1‑150 (MQ=255)
                                                                                                                                                 cTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCCCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCt     >  2:1019203/1‑150 (MQ=255)
                                                                                                                                                    gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCg  <  1:1549969/150‑1 (MQ=255)
                                                                                                                                                    gTGATAAAATTCACGATCCACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGGTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCg  <  1:574295/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCG  >  NC_000913/184092‑184389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: