Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 201,032 G→A 5.7% G21D (GGC→GAC)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913201,0320GA 5.7% 373.3 / 6.3 122G21D (GGC→GAC) lpxDUDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (62/53);  new base A (3/4);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 125 total aligned reads displayed.

CTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAA  >  NC_000913/200884‑201181
                                                                                                                                                    |                                                                                                                                                     
cTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGc                                                                                                                                                      <  1:1273457/150‑1 (MQ=255)
 tGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCg                                                                                                                                                     <  2:1691283/150‑1 (MQ=255)
       ttgATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCg                                                                                                                                               >  1:360078/1‑150 (MQ=255)
          aTGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCTTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGtca                                                                                                                                            >  1:1583933/1‑150 (MQ=255)
          aTGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTTGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGtca                                                                                                                                            >  1:800918/1‑150 (MQ=255)
             cAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGtcatcc                                                                                                                                         >  2:980306/1‑149 (MQ=255)
              aaaCGCCGTTGCTTACAACAGCAGCGATGTAAAACACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGACGATATCGCCATCAc                                                                                                                                        <  2:472368/150‑1 (MQ=255)
                aCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCg                                                                                                                                      <  2:1292427/150‑1 (MQ=255)
                aCGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCg                                                                                                                                      <  2:30967/150‑1 (MQ=255)
                  gCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGc                                                                                                                                    >  1:846461/1‑150 (MQ=255)
                  gCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGAGATCGTCATCACCGGc                                                                                                                                    >  2:1342619/1‑150 (MQ=255)
                  gCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGACGATATCGCCATCACCgtg                                                                                                                                    <  2:853019/150‑3 (MQ=255)
                   ccGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCg                                                                                                                                   <  1:577608/150‑1 (MQ=255)
                   ccGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCg                                                                                                                                   <  1:119972/150‑1 (MQ=255)
                   ccGCTGCTTACAACACCAGCGATGTACAAGACCTCACTGCCGACCTACTCAAACAGGTAAAATAACAAATGCCTCCAATTCGACTGGCTGATTTACCCCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCg                                                                                                                                   <  2:1864437/150‑1 (MQ=255)
                    cGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGACGATATCGCCATCACCgtgta                                                                                                                                  <  1:764089/150‑5 (MQ=255)
                         cTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGt                                                                                                                             >  1:1722642/1‑150 (MQ=255)
                         cTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGt                                                                                                                             >  2:1148914/1‑150 (MQ=255)
                               acaGCAGCGATGTAAAAGACATCACTGCCGACGTACTGATACAGGGTAAATAAGTATAGCCTTCAATTCGAGTGGCTGATTTAGAGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGACATCACCGGCGATGCGTCCATgg                                                                                                                       >  2:89761/1‑149 (MQ=255)
                               acaGAAGCTATGTAAAAGAAATCACTGCGGAAGTACTTAAACAGGTTAAATAAGTATTGGCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATTCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGc                                                                                                                       <  2:554401/150‑1 (MQ=255)
                                    agcGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTTGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCt                                                                                                                  <  2:800918/150‑1 (MQ=255)
                                       gATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGca                                                                                                               >  2:1351526/1‑150 (MQ=255)
                                       gATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGca                                                                                                               >  1:1283384/1‑150 (MQ=255)
                                           tAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACaaa                                                                                                           >  2:1555385/1‑150 (MQ=255)
                                                 aCATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTc                                                                                                     <  2:360078/150‑1 (MQ=255)
                                                  cATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTca                                                                                                    >  1:612963/1‑150 (MQ=255)
                                                   aTCACTGCCGACGTACTGAAACAGGTTAAATAAGTTATGCCTTCAATTCGACTGGCGGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTcac                                                                                                   >  1:247207/1‑150 (MQ=255)
                                                      aCTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACAtt                                                                                                >  1:743938/1‑150 (MQ=255)
                                                       cTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTa                                                                                               >  2:75913/1‑150 (MQ=255)
                                                       cTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTa                                                                                               <  1:1226531/150‑1 (MQ=255)
                                                       cTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTACCATTa                                                                                               >  1:323838/1‑150 (MQ=255)
                                                        tGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGAGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTAc                                                                                              >  1:315422/1‑150 (MQ=255)
                                                         gCCGTCGTACTGAAACAGGTTAAATAAGACATGCCTTCAATTCGACTGGCTGAGTTAGCGCACCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTAATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACg                                                                                             <  1:1127174/150‑1 (MQ=255)
                                                         gCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGCTCACATTACg                                                                                             >  2:263464/1‑150 (MQ=255)
                                                         gCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAAACTGCACAAACAGGTCACATTACg                                                                                             >  1:1782248/1‑150 (MQ=255)
                                                          ccGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGt                                                                                            <  1:466526/150‑1 (MQ=255)
                                                           cGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGtt                                                                                           <  1:1767030/150‑1 (MQ=255)
                                                            gACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTc                                                                                          <  2:1780770/150‑1 (MQ=255)
                                                            gACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTc                                                                                          <  2:1728228/150‑1 (MQ=255)
                                                               gTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATg                                                                                       <  2:518912/150‑1 (MQ=255)
                                                                tACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGTCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATgg                                                                                      <  1:469097/150‑1 (MQ=255)
                                                                 aCTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGt                                                                                     <  1:1449126/150‑1 (MQ=255)
                                                                 aCTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGt                                                                                     <  2:612963/150‑1 (MQ=255)
                                                                  cTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGtt                                                                                    <  2:19223/150‑1 (MQ=255)
                                                                   tGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTa                                                                                   >  1:647603/1‑150 (MQ=255)
                                                                    gAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTaa                                                                                  >  2:1167405/1‑150 (MQ=255)
                                                                    gAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTaa                                                                                  >  1:233524/1‑150 (MQ=255)
                                                                    gAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTaa                                                                                  >  2:791070/1‑150 (MQ=255)
                                                                    gAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTaa                                                                                  >  2:102822/1‑150 (MQ=255)
                                                                    gAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGAATTAGCGCAGCAGTTGGATGCAGAACAAAACGGTGATGGCGATATCGTCCTCACCGGCGTTGCGTACATGCAATCTGCACAAACAGGTCACATTACGTTGATTGTTaa                                                                                  >  2:1662737/1‑150 (MQ=255)
                                                                     aaaCAGGTTATATAATTAATGCCTTCAATTCGACTGGCTTATTTAGCGCAGCAGTTGGATGCAGAACTACACTGTTATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTaaa                                                                                 <  1:1330858/150‑2 (MQ=255)
                                                                     aaaCAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAAc                                                                                 <  1:1330855/150‑1 (MQ=255)
                                                                      aaCAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCGGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAAcc                                                                                <  2:225231/150‑1 (MQ=255)
                                                                        cAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCa                                                                              >  2:202150/1‑150 (MQ=255)
                                                                           gTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCaaaa                                                                           >  1:1683026/1‑150 (MQ=255)
                                                                           gTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCaaaa                                                                           <  1:340427/150‑1 (MQ=255)
                                                                              aaaTAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGCTGCAGACCTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACCGTTCAAATTACGTTCTTGGTTAACCCAAAATAc                                                                        >  2:148797/1‑150 (MQ=255)
                                                                              aaaTAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATAc                                                                        >  1:1833848/1‑150 (MQ=255)
                                                                                aTAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCg                                                                      >  2:1003018/1‑150 (MQ=255)
                                                                                    gTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAg                                                                  <  2:1488307/150‑1 (MQ=255)
                                                                                    gTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAg                                                                  <  1:1250948/150‑1 (MQ=255)
                                                                                        tGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGAAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTAACATTACGTTCATGTTTAACCCAAAATACAGTGAGCAtt                                                              >  2:585390/1‑150 (MQ=255)
                                                                                          ccTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTa                                                            >  1:694487/1‑150 (MQ=255)
                                                                                           cTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAg                                                           >  1:852026/1‑150 (MQ=255)
                                                                                             tCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGc                                                         <  1:958072/150‑1 (MQ=255)
                                                                                               aaTTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCtt                                                       >  1:1003316/1‑150 (MQ=255)
                                                                                                     aCTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACTGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCa                                                 <  1:1401825/150‑1 (MQ=255)
                                                                                                               ttAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCcgcg                                       <  2:1722642/150‑1 (MQ=255)
                                                                                                                 aGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGt                                     <  1:1306357/150‑1 (MQ=255)
                                                                                                                  gcgcAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGtt                                    >  1:742839/1‑150 (MQ=255)
                                                                                                                  gcgcAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGtt                                    >  2:1729549/1‑150 (MQ=255)
                                                                                                                   cgcAGCAGTTGGATGCAGAACTACACTGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTg                                   <  1:83539/150‑1 (MQ=255)
                                                                                                                   cgcAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGGCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATATCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTg                                   <  1:479806/150‑1 (MQ=255)
                                                                                                                     cagcagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTc                                 >  1:601512/1‑150 (MQ=255)
                                                                                                                     cagcagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTc                                 <  2:846461/150‑1 (MQ=255)
                                                                                                                     cagcagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTc                                 <  2:359323/150‑1 (MQ=255)
                                                                                                                     cagcagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATAACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTc                                 <  2:1183481/150‑1 (MQ=255)
                                                                                                                       gcagTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCag                               >  2:707190/1‑149 (MQ=255)
                                                                                                                         agTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACACACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGGTGTCATGa                             >  2:1568384/1‑150 (MQ=255)
                                                                                                                          gTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGAc                            <  2:233524/150‑1 (MQ=255)
                                                                                                                             ggATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCa                         >  1:821726/1‑150 (MQ=255)
                                                                                                                                      aCTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCt                <  2:1294741/150‑1 (MQ=255)
                                                                                                                                        tACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTc              <  2:1178387/150‑1 (MQ=255)
                                                                                                                                        tACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTc              >  1:756534/1‑150 (MQ=255)
                                                                                                                                        tACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTc              >  2:567326/1‑150 (MQ=255)
                                                                                                                                        acgccGGTGATGACGATATCGCCATCCCCGGCGTTGCGTCCATGCAATCTGCACAACCAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGCCCCAGGCCGATCTTc              >  1:790031/5‑150 (MQ=255)
                                                                                                                                         acacGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTcc             <  2:634490/150‑1 (MQ=255)
                                                                                                                                         acacCGAGATGGCGATATCGTAATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTcc             <  2:1669586/150‑1 (MQ=255)
                                                                                                                                          gccGGTGATGACGATATCGCCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCTTGACCCATGACGATCTTCCt            >  2:1404047/3‑150 (MQ=255)
                                                                                                                                           acGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCAAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCtt           >  1:1692204/1‑150 (MQ=255)
                                                                                                                                            cGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGGCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCttt          <  2:1015080/150‑1 (MQ=255)
                                                                                                                                              gTGCTGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTcg        <  1:1342619/150‑1 (MQ=255)
                                                                                                                                               tGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGCCCCAGGACGATCTTCCTTTcgc       >  1:101282/1‑150 (MQ=255)
                                                                                                                                               tGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGGTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTcgc       <  1:201898/150‑1 (MQ=255)
                                                                                                                                                gATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTcgcg      <  1:791070/150‑1 (MQ=255)
                                                                                                                                                gATGGCGATATCGTCATCACAGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACAATACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTCTCATGACCCAGGACGATGTTCCTTTcgcg      >  2:679815/1‑150 (MQ=255)
                                                                                                                                                  tGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGaa    >  1:66709/1‑150 (MQ=255)
                                                                                                                                                   ggCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGCGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGTCCCAGGACGATCTTCCTTTCGCGaaa   >  2:685868/1‑150 (MQ=255)
                                                                                                                                                   ggCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGaaa   >  2:1394792/1‑150 (MQ=255)
                                                                                                                                                    gCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGaaaa  >  2:811247/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAA  >  NC_000913/200884‑201181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: