Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,587,006 A→C 8.3% T426P (ACG→CCG) ‡ narQ → sensory histidine kinase in two‑component regulatory system with NarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,587,0060AC 8.3% 291.2 / 15.3 109T426P (ACG→CCG) ‡narQsensory histidine kinase in two‑component regulatory system with NarP
Reads supporting (aligned to +/- strand):  ref base A (40/60);  new base C (6/3);  total (46/63)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 110 total aligned reads displayed.

TACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCG  >  NC_000913/2586857‑2587154
                                                                                                                                                     |                                                                                                                                                    
tACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCTCAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCTCCTGa                                                                                                                                                      >  1:314250/1‑150 (MQ=255)
       ttacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgca                                                                                                                                               <  2:1107490/150‑1 (MQ=255)
        tccttacGTCTCCAGTTGACGTTACTGAAGCGGTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATCATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTATCCGCCTGACGCTgcag                                                                                                                                              <  2:1622832/148‑1 (MQ=255)
        tccttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                              <  1:1156119/148‑1 (MQ=255)
        tacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                              >  1:418940/1‑150 (MQ=255)
          cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                            >  1:523326/1‑150 (MQ=255)
          cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                            >  2:1164297/1‑150 (MQ=255)
            tacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGAGTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAgg                                                                                                                                          >  1:138618/1‑150 (MQ=255)
               gcaTCCAGTTGACGTTCCTGACGCGTTCGATCCCGGAAGCTCACGCCACCGCACAAAGGATCATGGCCGATTTTTCCCCGGCGTTGAATGAGGCTTCGCGGCAGGTACGCGAGCTGTTGACTACTTTCCGCCTGACGCGGCAGCAGGCgg                                                                                                                                       <  1:1158875/148‑1 (MQ=255)
               gTATCCAGTTGACGTTACTGCAGCGTTCGATACCCGAAGATACCGCCACCGCACAAAGTATCCTGGCCGATTTTTCCCAGGCGTTGAATGATGCATATCGGCAGTTACGCGAGCTGTGGACTACTTTCCGCCTGACGCTGCAGCAGGCgg                                                                                                                                       <  2:673205/150‑1 (MQ=255)
               gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCgg                                                                                                                                       <  1:1045772/150‑1 (MQ=255)
               gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCgg                                                                                                                                       <  2:1484484/150‑1 (MQ=255)
                 aTCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaa                                                                                                                                     <  1:863873/150‑2 (MQ=255)
                  tCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtc                                                                                                                                    <  2:1184012/150‑1 (MQ=255)
                    cAGTTGACGTTACTGAAGCGTTCGATACTGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCGGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaaagt                                                                                                                                  <  2:1667940/150‑5 (MQ=255)
                    cAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtctc                                                                                                                                  >  2:377555/1‑150 (MQ=255)
                    cAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTGGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtctc                                                                                                                                  <  1:42438/150‑1 (MQ=255)
                     aGTTGACGTTACTGAAGCGTGCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTcc                                                                                                                                 <  2:1510311/150‑1 (MQ=255)
                      gTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCAGATCTccc                                                                                                                                <  2:1737859/150‑1 (MQ=255)
                      gTTGACGTTACTGAAGCGTGCTATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTccc                                                                                                                                <  1:213356/150‑1 (MQ=255)
                           cgaaaCTGACGCGTTCGCTACCGGACGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCg                                                                                                                           <  2:147269/146‑1 (MQ=255)
                           cGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCg                                                                                                                           <  2:269287/150‑1 (MQ=255)
                              aaccgAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAt                                                                                                                        <  1:1494916/146‑1 (MQ=255)
                                cTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTg                                                                                                                      >  2:534912/1‑150 (MQ=255)
                                 tGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGa                                                                                                                     >  2:777128/1‑150 (MQ=255)
                                  gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                    >  2:1436303/1‑150 (MQ=255)
                                   caGCGTTCGATACCGGAAGATGACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAgg                                                                                                                   <  1:205333/149‑1 (MQ=255)
                                    aGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATGTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAggg                                                                                                                  <  2:897708/150‑1 (MQ=255)
                                     gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATTGCCGATTTTTCCCAGGCGTTGATTGATGCTTATCGGCAGTAACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGCCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                 >  1:134427/1‑150 (MQ=255)
                                     gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                 >  2:1767317/1‑150 (MQ=255)
                                      cGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGaa                                                                                                                >  2:138268/1‑150 (MQ=255)
                                       gTTCGATACAGGAAGATAACGCCACCGCACAAAGTATCATCGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGAGAGCTGTTGAATACTTTCCGCCTGACCCTGCAGCAGGAGGATCTCCCATCCGCATTTAGGGaaa                                                                                                               >  1:1588735/1‑150 (MQ=255)
                                        ttCGATACCGGAAGATAAAGCCACCTCACAAAGTATCTTGGCCGATTTTTGCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAAt                                                                                                              <  1:1306737/150‑1 (MQ=255)
                                         tCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCATATCAGCACTTACCCGAGCTGTAGACAACTTTCCGCCTGACGCTGCAGCTGGAGGCTCTCCCCTCCGCATTGAGGGAAATg                                                                                                             >  1:305279/1‑150 (MQ=255)
                                          cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGc                                                                                                            >  2:1490259/1‑150 (MQ=255)
                                            aTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTg                                                                                                          <  1:1770916/150‑1 (MQ=255)
                                             tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTgg                                                                                                         <  1:219979/150‑1 (MQ=255)
                                             tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTgg                                                                                                         <  1:1003471/150‑1 (MQ=255)
                                              aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                        <  2:1056259/150‑1 (MQ=255)
                                              aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGAGTTTTCCCAGGCGTTGAATGATGCATATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                        <  2:143507/150‑1 (MQ=255)
                                               ccGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGAt                                                                                                       >  2:1607713/1‑150 (MQ=255)
                                                    aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                  >  1:1748618/1‑150 (MQ=255)
                                                    aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                  <  1:890805/150‑1 (MQ=255)
                                                    aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                  <  2:1003009/150‑1 (MQ=255)
                                                    aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                  >  2:392198/1‑150 (MQ=255)
                                                          cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaaaa                                                                                            >  1:1498081/1‑150 (MQ=255)
                                                          cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaaaa                                                                                            <  2:1573269/150‑1 (MQ=255)
                                                           gCCACCGCCCAAAGGATCATGGCCGATTTTTCCCCGGCGGTGAATGATGCTTAGCGGCAGTTACGCGAGCTGTTGCCTTCTTTGCGCCTGACGCTGCAGCAGGCGGATCTCGCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAAt                                                                                           <  2:1134403/150‑1 (MQ=255)
                                                            ccACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATc                                                                                          <  1:1471246/150‑1 (MQ=255)
                                                                 gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                     <  2:218942/150‑1 (MQ=255)
                                                                    caAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTCAGGGAAATGCTGGATACGTTACAAAATCAAACCAgc                                                                                  >  2:624401/1‑150 (MQ=255)
                                                                              tGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGa                                                                        <  2:789609/150‑1 (MQ=255)
                                                                               ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCACACTGAc                                                                       >  1:289114/1‑150 (MQ=255)
                                                                               ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATGGAGGGAAATGCGGGATACGTTACAAAATCAAACCAGCGCCAAACTGAc                                                                       <  1:905145/150‑1 (MQ=255)
                                                                                gCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAcc                                                                      >  1:871514/1‑150 (MQ=255)
                                                                                gCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAcc                                                                      >  1:230471/1‑150 (MQ=255)
                                                                                 ccGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCCGCGCCAAACTGAccc                                                                     >  1:676090/1‑150 (MQ=255)
                                                                                 ccGATTTTTACCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAccc                                                                     <  1:123346/150‑1 (MQ=255)
                                                                                  ccaTTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTCACGCTGCAGCAGGCGGATCTCCACACCGCATTGAGGGAAATGCTGTATACGTTCAAAAGCAAACCAGCGCCAAACTCACCCTc                                                                   >  2:343989/3‑150 (MQ=255)
                                                                                    aTTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGATGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCg                                                                  >  2:234367/1‑150 (MQ=255)
                                                                                           ccAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCg                                                           >  2:413341/1‑150 (MQ=255)
                                                                                            cAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCATCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGt                                                          <  2:214920/150‑1 (MQ=255)
                                                                                             agtcGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTc                                                         <  2:1804826/147‑1 (MQ=255)
                                                                                             aGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTc                                                         <  2:523326/150‑1 (MQ=255)
                                                                                                cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAACCTGACCCTCGACTGCCGTCTGc                                                      >  2:422831/1‑150 (MQ=255)
                                                                                                cGTTGAATGATGCGTATCGGCAGTTACGCTAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGc                                                      <  2:314250/150‑1 (MQ=255)
                                                                                                      atgatgCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAccc                                                <  2:405678/150‑1 (MQ=255)
                                                                                                        gatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTg                                              <  1:1431451/150‑1 (MQ=255)
                                                                                                         atgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTgg                                             <  1:534912/150‑1 (MQ=255)
                                                                                                          tgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTAACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGc                                            <  1:99594/150‑1 (MQ=255)
                                                                                                           gCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCa                                           <  1:1436303/150‑1 (MQ=255)
                                                                                                             ttATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACt                                         <  1:851844/150‑1 (MQ=255)
                                                                                                             ttATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACt                                         <  2:1495353/150‑1 (MQ=255)
                                                                                                              tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                        <  2:1830519/150‑1 (MQ=255)
                                                                                                                 cGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGAt                                     <  1:1164297/150‑1 (MQ=255)
                                                                                                                    cAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgcg                                  >  1:1874096/1‑150 (MQ=255)
                                                                                                                    cAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgcg                                  <  1:400300/150‑1 (MQ=255)
                                                                                                                    cAGTTACGCGAGCGGGTGACTACTTGCCGCCTGACGCTGCAGCATGCGGATCTCCCCTCCGCAGTGAGGGAACTGCTGGATACGTTACACAATCACACCCGCGCCAAACTGACCCTCGCCTGCCGTCTGCCAACCCTGGCACTGGATgcg                                  <  1:1282791/150‑1 (MQ=255)
                                                                                                                      gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCa                                <  1:799763/150‑1 (MQ=255)
                                                                                                                      gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCa                                <  1:259841/150‑1 (MQ=255)
                                                                                                                          cgcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATg                            <  2:912418/150‑1 (MQ=255)
                                                                                                                            cgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCa                          <  2:138618/150‑1 (MQ=255)
                                                                                                                               gCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGt                       <  1:223386/150‑1 (MQ=255)
                                                                                                                                  gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACTTTACAAAATCAAACCAGCGCCAAACTGACCCTCCACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCa                    >  2:1006087/1‑150 (MQ=255)
                                                                                                                                  gTTGACTACTTTCCGCCTGACGCGGCAGCAGGCGGATCTCCCCTCCGCATTGTGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCa                    <  1:779687/150‑1 (MQ=255)
                                                                                                                                      actactTTCCGCCTGACGCTTCAGAAGGCGGATCTACACTCCGCATTGAGGTAAATGGTGGATACTTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACAGCCGTCTGCCACCCCTGGCACTGGATGCGCAATAGCAGGTGACTttg                >  2:1157906/1‑150 (MQ=255)
                                                                                                                                      actactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCAtttt                >  2:1726138/1‑149 (MQ=255)
                                                                                                                                      actactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttg                >  2:1012633/1‑150 (MQ=255)
                                                                                                                                        tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt              <  2:923304/150‑1 (MQ=255)
                                                                                                                                        tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCACCTGCATttgcg              <  2:1383330/150‑3 (MQ=255)
                                                                                                                                        ggagatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt              >  2:1391454/6‑150 (MQ=255)
                                                                                                                                        aactTTCCTCCTGACGCTTCCGCAGGCGGATCTCCCCTCAGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGTCACTGGATGCGCAAATGCAGGTGCATttgtt              <  1:1189666/149‑1 (MQ=255)
                                                                                                                                         gagatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgttg             >  2:571813/5‑150 (MQ=255)
                                                                                                                                           tttCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa           >  2:958863/1‑150 (MQ=255)
                                                                                                                                           gatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATCCAGGTGCATTTGTTGCa           >  1:444575/3‑150 (MQ=255)
                                                                                                                                           gatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTAACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa           >  1:1095532/3‑150 (MQ=255)
                                                                                                                                              ccGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAat        <  1:399037/150‑1 (MQ=255)
                                                                                                                                               cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt       >  2:1033967/1‑150 (MQ=255)
                                                                                                                                                  cTGACGCTGCAGCAGGCGCATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTCACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAattatt    <  2:1748618/150‑1 (MQ=255)
                                                                                                                                                    gACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcg  <  2:338973/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCG  >  NC_000913/2586857‑2587154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: