Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,587,007 C→T 8.2% T426M (ACG→ATG) ‡ narQ → sensory histidine kinase in two‑component regulatory system with NarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,587,0070CT 8.2% 329.8 / 11.7 109T426M (ACG→ATG) ‡narQsensory histidine kinase in two‑component regulatory system with NarP
Reads supporting (aligned to +/- strand):  ref base C (40/60);  new base T (6/3);  total (46/63)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCG  >  NC_000913/2586863‑2587156
                                                                                                                                                |                                                                                                                                                     
 ttacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgca                                                                                                                                                 <  2:1107490/150‑1 (MQ=255)
  tccttacGTCTCCAGTTGACGTTACTGAAGCGGTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATCATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTATCCGCCTGACGCTgcag                                                                                                                                                <  2:1622832/148‑1 (MQ=255)
  tccttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                                <  1:1156119/148‑1 (MQ=255)
  tacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                                >  1:418940/1‑150 (MQ=255)
    cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                              >  2:1164297/1‑150 (MQ=255)
    cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                              >  1:523326/1‑150 (MQ=255)
      tacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGAGTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAgg                                                                                                                                            >  1:138618/1‑150 (MQ=255)
         gcaTCCAGTTGACGTTCCTGACGCGTTCGATCCCGGAAGCTCACGCCACCGCACAAAGGATCATGGCCGATTTTTCCCCGGCGTTGAATGAGGCTTCGCGGCAGGTACGCGAGCTGTTGACTACTTTCCGCCTGACGCGGCAGCAGGCgg                                                                                                                                         <  1:1158875/148‑1 (MQ=255)
         gTATCCAGTTGACGTTACTGCAGCGTTCGATACCCGAAGATACCGCCACCGCACAAAGTATCCTGGCCGATTTTTCCCAGGCGTTGAATGATGCATATCGGCAGTTACGCGAGCTGTGGACTACTTTCCGCCTGACGCTGCAGCAGGCgg                                                                                                                                         <  2:673205/150‑1 (MQ=255)
         gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCgg                                                                                                                                         <  1:1045772/150‑1 (MQ=255)
         gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCgg                                                                                                                                         <  2:1484484/150‑1 (MQ=255)
           aTCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaa                                                                                                                                       <  1:863873/150‑2 (MQ=255)
           aTCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCATCAGGCGGAt                                                                                                                                       >  1:338973/1‑150 (MQ=255)
            tCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtc                                                                                                                                      <  2:1184012/150‑1 (MQ=255)
              cAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtctc                                                                                                                                    >  2:377555/1‑150 (MQ=255)
              cAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTGGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAtctc                                                                                                                                    <  1:42438/150‑1 (MQ=255)
               aGTTGACGTTACTGAAGCGTGCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTcc                                                                                                                                   <  2:1510311/150‑1 (MQ=255)
                gTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCAGATCTccc                                                                                                                                  <  2:1737859/150‑1 (MQ=255)
                gTTGACGTTACTGAAGCGTGCTATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTccc                                                                                                                                  <  1:213356/150‑1 (MQ=255)
                     cgaaaCTGACGCGTTCGCTACCGGACGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCg                                                                                                                             <  2:147269/146‑1 (MQ=255)
                     cGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCg                                                                                                                             <  2:269287/150‑1 (MQ=255)
                        aaccgAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAt                                                                                                                          <  1:1494916/146‑1 (MQ=255)
                          cTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTg                                                                                                                        >  2:534912/1‑150 (MQ=255)
                           tGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGa                                                                                                                       >  2:777128/1‑150 (MQ=255)
                            gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                      >  2:1436303/1‑150 (MQ=255)
                             caGCGTTCGATACCGGAAGATGACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAgg                                                                                                                     <  1:205333/149‑1 (MQ=255)
                              aGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATGTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAggg                                                                                                                    <  2:897708/150‑1 (MQ=255)
                               gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATTGCCGATTTTTCCCAGGCGTTGATTGATGCTTATCGGCAGTAACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGCCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                   >  1:134427/1‑150 (MQ=255)
                               gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                   >  2:1767317/1‑150 (MQ=255)
                                cGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGaa                                                                                                                  >  2:138268/1‑150 (MQ=255)
                                 gTTCGATACAGGAAGATAACGCCACCGCACAAAGTATCATCGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGAGAGCTGTTGAATACTTTCCGCCTGACCCTGCAGCAGGAGGATCTCCCATCCGCATTTAGGGaaa                                                                                                                 >  1:1588735/1‑150 (MQ=255)
                                  ttCGATACCGGAAGATAAAGCCACCTCACAAAGTATCTTGGCCGATTTTTGCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAAt                                                                                                                <  1:1306737/150‑1 (MQ=255)
                                   tCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCATATCAGCACTTACCCGAGCTGTAGACAACTTTCCGCCTGACGCTGCAGCTGGAGGCTCTCCCCTCCGCATTGAGGGAAATg                                                                                                               >  1:305279/1‑150 (MQ=255)
                                    cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGc                                                                                                              >  2:1490259/1‑150 (MQ=255)
                                      aTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTg                                                                                                            <  1:1770916/150‑1 (MQ=255)
                                       tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGCTGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCtcg                                                                                                           >  2:164800/1‑148 (MQ=255)
                                       tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTgg                                                                                                           <  1:1003471/150‑1 (MQ=255)
                                       tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTgg                                                                                                           <  1:219979/150‑1 (MQ=255)
                                         ccGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGAt                                                                                                         >  2:1607713/1‑150 (MQ=255)
                                              aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                    <  1:890805/150‑1 (MQ=255)
                                              aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                    <  2:1003009/150‑1 (MQ=255)
                                              aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                    >  1:1748618/1‑150 (MQ=255)
                                              aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                    >  2:392198/1‑150 (MQ=255)
                                                    cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaaaa                                                                                              <  2:1573269/150‑1 (MQ=255)
                                                    cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaaaa                                                                                              >  1:1498081/1‑150 (MQ=255)
                                                     gCCACCGCCCAAAGGATCATGGCCGATTTTTCCCCGGCGGTGAATGATGCTTAGCGGCAGTTACGCGAGCTGTTGCCTTCTTTGCGCCTGACGCTGCAGCAGGCGGATCTCGCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAAt                                                                                             <  2:1134403/150‑1 (MQ=255)
                                                      ccACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATc                                                                                            <  1:1471246/150‑1 (MQ=255)
                                                           gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                       <  2:218942/150‑1 (MQ=255)
                                                              caAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTCAGGGAAATGCTGGATACGTTACAAAATCAAACCAgc                                                                                    >  2:624401/1‑150 (MQ=255)
                                                                        tGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGa                                                                          <  2:789609/150‑1 (MQ=255)
                                                                         ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCACACTGAc                                                                         >  1:289114/1‑150 (MQ=255)
                                                                         ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATGGAGGGAAATGCGGGATACGTTACAAAATCAAACCAGCGCCAAACTGAc                                                                         <  1:905145/150‑1 (MQ=255)
                                                                          gCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAcc                                                                        >  1:230471/1‑150 (MQ=255)
                                                                          gCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAcc                                                                        >  1:871514/1‑150 (MQ=255)
                                                                           ccGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCCGCGCCAAACTGAccc                                                                       >  1:676090/1‑150 (MQ=255)
                                                                           ccGATTTTTACCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAccc                                                                       <  1:123346/150‑1 (MQ=255)
                                                                            ccaTTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTCACGCTGCAGCAGGCGGATCTCCACACCGCATTGAGGGAAATGCTGTATACGTTCAAAAGCAAACCAGCGCCAAACTCACCCTc                                                                     >  2:343989/3‑150 (MQ=255)
                                                                              aTTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGATGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCg                                                                    >  2:234367/1‑150 (MQ=255)
                                                                                     ccAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCg                                                             >  2:413341/1‑150 (MQ=255)
                                                                                      cAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCATCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGt                                                            <  2:214920/150‑1 (MQ=255)
                                                                                       agtcGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTc                                                           <  2:1804826/147‑1 (MQ=255)
                                                                                       aGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTc                                                           <  2:523326/150‑1 (MQ=255)
                                                                                          cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAACCTGACCCTCGACTGCCGTCTGc                                                        >  2:422831/1‑150 (MQ=255)
                                                                                          cGTTGAATGATGCGTATCGGCAGTTACGCTAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGc                                                        <  2:314250/150‑1 (MQ=255)
                                                                                           gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGcc                                                       <  2:1044070/150‑1 (MQ=255)
                                                                                                atgatgCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAccc                                                  <  2:405678/150‑1 (MQ=255)
                                                                                                  gatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTg                                                <  1:1431451/150‑1 (MQ=255)
                                                                                                   atgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTgg                                               <  1:534912/150‑1 (MQ=255)
                                                                                                    tgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTAACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGc                                              <  1:99594/150‑1 (MQ=255)
                                                                                                     gCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCa                                             <  1:1436303/150‑1 (MQ=255)
                                                                                                       ttATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACt                                           <  2:1495353/150‑1 (MQ=255)
                                                                                                        tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                          <  2:1830519/150‑1 (MQ=255)
                                                                                                         aTCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTgg                                         >  1:846539/1‑150 (MQ=255)
                                                                                                           cGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGAt                                       <  1:1164297/150‑1 (MQ=255)
                                                                                                              cAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgcg                                    >  1:1874096/1‑150 (MQ=255)
                                                                                                              cAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgcg                                    <  1:400300/150‑1 (MQ=255)
                                                                                                              cAGTTACGCGAGCGGGTGACTACTTGCCGCCTGACGCTGCAGCATGCGGATCTCCCCTCCGCAGTGAGGGAACTGCTGGATACGTTACACAATCACACCCGCGCCAAACTGACCCTCGCCTGCCGTCTGCCAACCCTGGCACTGGATgcg                                    <  1:1282791/150‑1 (MQ=255)
                                                                                                                gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCa                                  <  1:799763/150‑1 (MQ=255)
                                                                                                                gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCa                                  <  1:259841/150‑1 (MQ=255)
                                                                                                                    cgcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATg                              <  2:912418/150‑1 (MQ=255)
                                                                                                                      cgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCa                            <  2:138618/150‑1 (MQ=255)
                                                                                                                         gCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGt                         <  1:223386/150‑1 (MQ=255)
                                                                                                                            gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACTTTACAAAATCAAACCAGCGCCAAACTGACCCTCCACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCa                      >  2:1006087/1‑150 (MQ=255)
                                                                                                                            gTTGACTACTTTCCGCCTGACGCGGCAGCAGGCGGATCTCCCCTCCGCATTGTGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCa                      <  1:779687/150‑1 (MQ=255)
                                                                                                                                actactTTCCGCCTGACGCTTCAGAAGGCGGATCTACACTCCGCATTGAGGTAAATGGTGGATACTTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACAGCCGTCTGCCACCCCTGGCACTGGATGCGCAATAGCAGGTGACTttg                  >  2:1157906/1‑150 (MQ=255)
                                                                                                                                actactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCAtttt                  >  2:1726138/1‑149 (MQ=255)
                                                                                                                                actactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttg                  >  2:1012633/1‑150 (MQ=255)
                                                                                                                                  tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                <  2:923304/150‑1 (MQ=255)
                                                                                                                                  tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCACCTGCATttgcg                <  2:1383330/150‑3 (MQ=255)
                                                                                                                                  ggagatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                >  2:1391454/6‑150 (MQ=255)
                                                                                                                                  ggagatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                >  2:1434806/6‑150 (MQ=255)
                                                                                                                                  aactTTCCTCCTGACGCTTCCGCAGGCGGATCTCCCCTCAGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGTCACTGGATGCGCAAATGCAGGTGCATttgtt                <  1:1189666/149‑1 (MQ=255)
                                                                                                                                   gagatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgttg               >  2:571813/5‑150 (MQ=255)
                                                                                                                                     tttCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa             >  2:958863/1‑150 (MQ=255)
                                                                                                                                     gatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATCCAGGTGCATTTGTTGCa             >  1:444575/3‑150 (MQ=255)
                                                                                                                                     gatCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTAACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa             >  1:1095532/3‑150 (MQ=255)
                                                                                                                                         cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt         >  2:1033967/1‑150 (MQ=255)
                                                                                                                                            cTGACGCTGCAGCAGGCGCATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTCACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAattatt      <  2:1748618/150‑1 (MQ=255)
                                                                                                                                              gACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcg    <  2:338973/150‑1 (MQ=255)
                                                                                                                                                cGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcgcg  >  2:258693/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCG  >  NC_000913/2586863‑2587156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: