Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,584 A→T 6.8% intergenic (‑49/+20) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5840AT 6.8% 395.9 / 10.2 140intergenic (‑49/+20)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (58/72);  new base T (5/5);  total (63/77)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAG  >  NC_000913/2632435‑2632733
                                                                                                                                                     |                                                                                                                                                     
cAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACa                                                                                                                                                       <  1:865681/150‑1 (MQ=255)
  gTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCCTTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATg                                                                                                                                                     <  2:644280/150‑1 (MQ=255)
   tAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGa                                                                                                                                                    <  1:584729/150‑1 (MQ=255)
       cacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATTCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGTCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTc                                                                                                                                                >  2:1449559/1‑150 (MQ=255)
       cacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTc                                                                                                                                                >  2:1718113/1‑150 (MQ=255)
       cacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTc                                                                                                                                                >  1:1787743/1‑150 (MQ=255)
        acCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCg                                                                                                                                               >  1:1679115/1‑150 (MQ=255)
          ccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCggg                                                                                                                                             <  1:954058/150‑1 (MQ=255)
           cAGCTCACGCCGTGGGTGCGGAACCAGTTGAGTGCACTGAGAACCTAAGTCAAGTATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGTAACAGATAATATAAATCGCCCGACATGAAGTCGGGc                                                                                                                                            <  2:1511280/150‑1 (MQ=255)
           cAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGc                                                                                                                                            <  1:1639877/150‑1 (MQ=255)
           cAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGAGGCGATGCTTATGAATTTTTTCCGTCATAGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                                                                            <  2:1825488/150‑1 (MQ=255)
           cAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACGGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCATATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                                                                            <  1:361574/150‑1 (MQ=255)
            cgCTCACGCACGCGGCGCGCAACCCGTTGAGTGTACTGCGCACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATCTAAATCGCCCGACTTCATGTCGGGCg                                                                                                                                           <  1:1540646/149‑1 (MQ=255)
            aGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCg                                                                                                                                           >  1:463797/1‑150 (MQ=255)
             gCTCAAGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGa                                                                                                                                          <  1:211105/150‑1 (MQ=255)
              cTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGat                                                                                                                                         <  1:1662081/150‑2 (MQ=255)
              cTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGaa                                                                                                                                         >  1:1293368/1‑150 (MQ=255)
                cacgcacgCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAaga                                                                                                                                       <  1:28254/150‑1 (MQ=255)
                   gcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCAGGCGAAGAGaa                                                                                                                                    >  2:1426042/1‑150 (MQ=255)
                      cgcgGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                                                                                 >  1:513799/1‑150 (MQ=255)
                        cggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                                                                               <  1:1637193/150‑1 (MQ=255)
                        cggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                                                                               <  1:543178/150‑1 (MQ=255)
                          gcgcgcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAg                                                                                                                             <  1:142020/150‑1 (MQ=255)
                             cgcAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGGCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGccc                                                                                                                          <  1:538621/150‑1 (MQ=255)
                                aaCCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGATTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGa                                                                                                                       >  2:341184/1‑150 (MQ=255)
                                  ccAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGATGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACg                                                                                                                     <  2:1287478/150‑1 (MQ=255)
                                   cAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACgg                                                                                                                    >  1:697946/1‑150 (MQ=255)
                                   cAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACgg                                                                                                                    >  1:1517781/1‑150 (MQ=255)
                                     gTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTa                                                                                                                  >  1:1251406/1‑150 (MQ=255)
                                        gAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGGCATTGACGCTTATTCCGAGTCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGtt                                                                                                               <  2:1800610/150‑1 (MQ=255)
                                          gtgtACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAACATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCg                                                                                                             >  2:461723/1‑150 (MQ=255)
                                            ctACTTAGAACCGAACTCCAGAATGAGTCTGGTATGCATATGAATGTTTTCCGTCATTGACGCTTATTCAGAGGCAATTGAAACAGATAATATAAATCGCCCTACATGAAGTCTGGCGAAGAGAATAAGGAGCCCAGACGGTAGTTCggg                                                                                                           <  2:1174411/149‑1 (MQ=255)
                                               cTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAc                                                                                                        >  1:1355301/1‑150 (MQ=255)
                                                tGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAct                                                                                                       >  2:481840/1‑150 (MQ=255)
                                                 gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGCCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                                                                      >  1:1383339/1‑150 (MQ=255)
                                                 gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                                                                      >  1:159437/1‑150 (MQ=255)
                                                   gaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATCAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCtt                                                                                                    <  1:17457/150‑1 (MQ=255)
                                                    aaCCGAAGTCCAGAATGCGGATGCGATGCTTATGAATGTTTTCCGTAATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                                                                   <  2:717853/150‑1 (MQ=255)
                                                      ccGAAGTCCAGAATGAGGATGTGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                 >  1:1722083/1‑150 (MQ=255)
                                                      ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                                                                                 <  2:1518758/150‑1 (MQ=255)
                                                       cGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGt                                                                                                <  2:758445/150‑1 (MQ=255)
                                                        gAAGCCCAGAATGAGGATGCGATGCTATAGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATCTAAAGCGCCCGACAGGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTa                                                                                               <  2:314472/150‑1 (MQ=255)
                                                           gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                            <  2:1293368/150‑1 (MQ=255)
                                                            tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                                                                           <  1:1006055/150‑1 (MQ=255)
                                                            tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                                                                           <  2:554868/150‑1 (MQ=255)
                                                             ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGt                                                                                          >  2:1779631/1‑150 (MQ=255)
                                                              cAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                                                                         >  1:798951/1‑150 (MQ=255)
                                                                      ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGTTCACGTCGTg                                                                                 >  2:1253828/1‑150 (MQ=255)
                                                                      ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTg                                                                                 <  2:649232/150‑1 (MQ=255)
                                                                      ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGGCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTg                                                                                 <  2:1791339/150‑1 (MQ=255)
                                                                         tgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                                                                              >  1:1443706/1‑150 (MQ=255)
                                                                         tgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                                                                              >  1:1025726/1‑150 (MQ=255)
                                                                          gcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACg                                                                             >  2:199426/1‑150 (MQ=255)
                                                                           cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGt                                                                            >  1:406639/1‑150 (MQ=255)
                                                                            tatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                           <  2:825534/149‑1 (MQ=255)
                                                                             atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTgg                                                                          <  1:1426632/150‑1 (MQ=255)
                                                                              tgcTTATGATTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGc                                                                         <  2:1765456/150‑1 (MQ=255)
                                                                                    tGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGTGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCt                                                                   >  1:48897/1‑150 (MQ=255)
                                                                                    tGAATGTTTTCCGTCATAGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCt                                                                   <  2:311949/150‑1 (MQ=255)
                                                                                     gAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                                                                  <  1:1035771/150‑1 (MQ=255)
                                                                                      aaTGTTTTCCGTCATTGACGCTTATTCCGAGCCAAGTGAAACAGATAATATAAATCGCCCGACATGACGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGGGGCTTTCCTGa                                                                 <  2:916334/150‑1 (MQ=255)
                                                                                       aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                                                <  1:1134209/150‑1 (MQ=255)
                                                                                             tCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg                                                          >  2:1427424/1‑150 (MQ=255)
                                                                                             tCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg                                                          <  1:1292530/150‑1 (MQ=255)
                                                                                              ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGc                                                         >  1:1121731/1‑150 (MQ=255)
                                                                                                    ttGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCt                                                   <  2:1355301/150‑1 (MQ=255)
                                                                                                     tGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTg                                                  <  2:1111910/150‑1 (MQ=255)
                                                                                                      gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGa                                                 >  2:1364032/1‑150 (MQ=255)
                                                                                                      gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTCCTGAATGCCCGCACCGCTGAt                                                <  1:1857945/150‑1 (MQ=255)
                                                                                                       aCGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGATGAGAATCAGTAGCCCAGAAGGTAGTTCGGGGACTCTTTAGTAATGTACACGTCGTGAACGAGGCTTTCCTGAATGCCCGAACCGCTGAt                                                >  1:459465/1‑150 (MQ=255)
                                                                                                          cTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCCCCGCTGATACg                                             >  1:1755813/1‑150 (MQ=255)
                                                                                                            tATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTa                                           <  1:203288/150‑1 (MQ=255)
                                                                                                              ttCCGAGGCAAGTGAAACAGATAATTTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCATGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACa                                         <  1:1535226/150‑1 (MQ=255)
                                                                                                              ttCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCATGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACa                                         <  1:1527866/150‑1 (MQ=255)
                                                                                                                    ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTAAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                   >  1:1632966/1‑150 (MQ=255)
                                                                                                                    ggCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCACACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                   <  2:256517/150‑1 (MQ=255)
                                                                                                                    ggCAAGTGAAACAGATAATATAAATCGACCGACATGAAGTCGGGCTAAGAGAATCAGGAGCCCATACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTTAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTcc                                   <  2:1069119/150‑1 (MQ=255)
                                                                                                                      cAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGc                                 <  2:1514682/150‑1 (MQ=255)
                                                                                                                      aaaGTGAAACATATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGc                                 <  2:215842/149‑1 (MQ=255)
                                                                                                                       aacttaaaCAGTTAATATTAATCGCCAGACATGAATTCGGGCGAAGAGAATCTGGATCCCAGACGGTAGATCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCGTGAATGCCCGCACCGCTGATACTTACAAACTCCGCt                                <  2:1679115/145‑1 (MQ=255)
                                                                                                                        aGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCtt                               >  1:765103/1‑150 (MQ=255)
                                                                                                                          tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTa                             <  2:615257/150‑1 (MQ=255)
                                                                                                                           gAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAg                            >  1:587070/1‑150 (MQ=255)
                                                                                                                            aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACTGTAGTTCGGGGACTCTTTAGTAATTGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           <  1:1208376/150‑1 (MQ=255)
                                                                                                                            aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           >  2:1666848/1‑150 (MQ=255)
                                                                                                                                  ataataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAg                     <  2:1343246/150‑1 (MQ=255)
                                                                                                                                   aaataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGt                    <  1:1451092/149‑1 (MQ=255)
                                                                                                                                     atatAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCCGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAAGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGGGTTAGTACGCTGTtc                  >  1:1253415/1‑150 (MQ=255)
                                                                                                                                        taAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAAAGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtc               >  2:672053/1‑150 (MQ=255)
                                                                                                                                        taAATCGCCCGAAATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtc               <  1:1696938/150‑1 (MQ=255)
                                                                                                                                         aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtcg              <  1:446365/150‑1 (MQ=255)
                                                                                                                                         aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgtcg              <  1:686805/150‑1 (MQ=255)
                                                                                                                                           ttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGAt            >  2:1609248/2‑150 (MQ=255)
                                                                                                                                             ctcccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAg          <  2:1214666/148‑1 (MQ=255)
                                                                                                                                             cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAg          <  2:159437/150‑1 (MQ=255)
                                                                                                                                              gCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGt         >  1:477671/1‑150 (MQ=255)
                                                                                                                                               cccGACATGAAGTCGGGCGAAGAGAATCAGCAGCCCAGACGTTAGTTCGGGGACTCGTTAGTAATGTTCACCTCGTGAGCGAGGCTTTCCTGAATGCCAGCACCGCTGAAACGTACAAACTCCGCTTTAGTACGCAGTTCGACGATAgga        >  1:767580/1‑148 (MQ=255)
                                                                                                                                                    cATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACcaca   <  1:461723/150‑1 (MQ=255)
                                                                                                                                                    cATGAAGTCGGGCGAAGAGAATCAGGAGCACAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTTAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTACTACGCAGTGCGTCGATAGTACcac  >  2:1559545/1‑149 (MQ=255)
                                                                                                                                                     aTGACGTCGGGCGAAGAGACTCAGGAGCCCAGCCGGTCGGTCGGGGACTCTATAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGACTGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAg  <  2:246396/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAG  >  NC_000913/2632435‑2632733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: