Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,660,612 T→A 5.3% Y307F (TAC→TTC)  iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,660,6120TA 5.3% 369.8 / 3.8 130Y307F (TAC→TTC) iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (71/50);  new base A (5/3);  total (77/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

AAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATG  >  NC_000913/2660463‑2660761
                                                                                                                                                     |                                                                                                                                                     
aaGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCTCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGt                                                                                                                                                       <  1:1402761/150‑1 (MQ=255)
  gagaAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAg                                                                                                                                                     >  1:1735298/1‑150 (MQ=255)
    gaAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGtt                                                                                                                                                   >  2:69954/1‑150 (MQ=255)
     aaaCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTg                                                                                                                                                  <  2:16265/150‑1 (MQ=255)
      aaCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGa                                                                                                                                                 >  1:457615/1‑150 (MQ=255)
          gATAGAGCTCTGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGTCTGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACTACGTTGAAggt                                                                                                                                             <  1:61188/150‑3 (MQ=255)
          gATAGAGCTATGTGCCAGCTCGTCGTTCCGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCTCCTCACCTTCAAAGAAGTTGAAGCt                                                                                                                                             >  1:267122/1‑150 (MQ=255)
          gATAGAGCTATGTGCCAGCTCGTCGTTCATCCCCAGCGCGCGCATCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGTCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACTACGTTGAAggt                                                                                                                                             <  1:183574/150‑3 (MQ=255)
           aTAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTg                                                                                                                                            >  1:634339/1‑150 (MQ=255)
              gagCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGCAACTGCGAGGTCTTTCCGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACg                                                                                                                                         >  1:1032093/1‑150 (MQ=255)
                  tATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTga                                                                                                                                     >  1:325093/1‑150 (MQ=255)
                   aTGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTgag                                                                                                                                    >  1:522078/1‑150 (MQ=255)
                       gCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGCAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGGTGAAGCTGACGTTGAGAATg                                                                                                                                >  2:941747/1‑150 (MQ=255)
                             tcgtcgTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgc                                                                                                                          >  2:964766/1‑150 (MQ=255)
                             tcgtcgTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgc                                                                                                                          >  1:541678/1‑150 (MQ=255)
                             gcgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgc                                                                                                                          <  1:1565819/149‑1 (MQ=255)
                              cgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgcg                                                                                                                         >  2:1660794/1‑150 (MQ=255)
                              cgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgcg                                                                                                                         >  2:1785303/1‑150 (MQ=255)
                                 cgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCAc                                                                                                                      >  2:925764/1‑150 (MQ=255)
                                  gtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCAcc                                                                                                                     >  2:35068/1‑150 (MQ=255)
                                     cAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCgtg                                                                                                                  >  2:1547961/1‑150 (MQ=255)
                                      aGCCCCAGCGCGCGCCGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATTATCCGCGACTCCCCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCgtgt                                                                                                                 >  2:110557/1‑150 (MQ=255)
                                      aGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGAACAGGCCGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCgtgt                                                                                                                 >  1:348377/1‑150 (MQ=255)
                                        ccccAGCGCGCGCAGCACGTAGGACGGTTGGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGCCTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTc                                                                                                               >  2:788344/1‑150 (MQ=255)
                                         cccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTcc                                                                                                              >  1:1393147/1‑150 (MQ=255)
                                          ccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCa                                                                                                             <  2:676655/150‑1 (MQ=255)
                                             gcgcgcgcAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGCCGGATCCTGAATAAACTGCGAGGTCTTTCAGCGACATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGt                                                                                                          >  2:106697/1‑150 (MQ=255)
                                                    cagcaCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGtt                                                                                                   >  2:1339828/1‑150 (MQ=255)
                                                    cagcaCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGtt                                                                                                   >  2:15780/1‑150 (MQ=255)
                                                       caCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAg                                                                                                <  2:1659598/150‑1 (MQ=255)
                                                        aCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAgg                                                                                               <  1:1227110/150‑1 (MQ=255)
                                                        aCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGAAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAgg                                                                                               <  1:1007898/150‑1 (MQ=255)
                                                         cGTAGGACGTTTCGAGGCGTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGTGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGt                                                                                              <  2:1179687/150‑1 (MQ=255)
                                                            aGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTaaa                                                                                           <  1:1419653/150‑1 (MQ=255)
                                                             ggACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc                                                                                          <  2:946152/150‑1 (MQ=255)
                                                             ggACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc                                                                                          >  1:242714/1‑150 (MQ=255)
                                                              gACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAct                                                                                         >  1:938349/1‑150 (MQ=255)
                                                                gggTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGGGACTCACCTTCACCGAAGTTGAAGCTGACGTTGAGATTGTTCTGAGCACCGTGATCCAGGTCACCGTTCAGGTAAActtc                                                                                       <  1:1481391/149‑1 (MQ=255)
                                                                  gtgcGAGGCGTGCTGACGTACAGCCGGAACCTGAAGAAACTGCGAGGGCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtctt                                                                                     <  1:114415/147‑1 (MQ=255)
                                                                  gTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtctt                                                                                     <  2:1472675/150‑1 (MQ=255)
                                                                  gTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtctt                                                                                     >  1:1755713/1‑150 (MQ=255)
                                                                  gTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTGCAGGTAATcttctt                                                                                     >  1:900642/1‑150 (MQ=255)
                                                                   ttCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc                                                                                    >  1:953085/1‑150 (MQ=255)
                                                                   ttCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc                                                                                    >  1:969067/1‑150 (MQ=255)
                                                                   ttCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc                                                                                    <  1:1372471/150‑1 (MQ=255)
                                                                    gcgcggCTTGCTGACGTACAGGCTGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGGTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTGCCAGGTCACCGTTCAGGTAAACTTCTTCg                                                                                   <  2:1194848/146‑1 (MQ=255)
                                                                      gAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGat                                                                                 <  1:1450955/150‑1 (MQ=255)
                                                                      gAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACATAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGGTCAGGTAAACTTCTTCGat                                                                                 >  2:1047678/1‑150 (MQ=255)
                                                                            tGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                                           >  2:333532/1‑150 (MQ=255)
                                                                             gCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTg                                                                          <  2:120967/150‑1 (MQ=255)
                                                                              cTGACGTACAGGCGGAACCTGAACAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                                         <  2:1223820/150‑1 (MQ=255)
                                                                                  cGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGCCGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATGTTTGATGcc                                                                     >  1:244272/1‑150 (MQ=255)
                                                                                   gTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCg                                                                    >  1:1707944/1‑150 (MQ=255)
                                                                                    tACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGt                                                                   >  1:683506/1‑150 (MQ=255)
                                                                                       aGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTcc                                                                <  2:703292/150‑1 (MQ=255)
                                                                                           ggAACCTCAAGAACCTGCGAGGTCTTTCAGCGCCAAAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCTCCGTGATCCAGGTCACCCTTAAGGTAAACTTCTTCGATAACATTGATGCCGTTAcacat                                                            >  2:319307/1‑149 (MQ=255)
                                                                                             aaCCTGAAGAAACTGCGAGGGCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGAc                                                          <  2:1032093/150‑1 (MQ=255)
                                                                                              aCCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACTTTGAGAATGTTAGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACg                                                         >  1:1571494/1‑150 (MQ=255)
                                                                                               ccTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACgg                                                        >  2:973523/1‑150 (MQ=255)
                                                                                                cTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGt                                                       >  1:168726/1‑150 (MQ=255)
                                                                                                cTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGGTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGCCGGt                                                       >  2:1026642/1‑150 (MQ=255)
                                                                                                   aagaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCACCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCTCCGTGTTCCAGGGCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTAc                                                    >  1:933851/1‑150 (MQ=255)
                                                                                                    agaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACg                                                   <  2:501301/150‑1 (MQ=255)
                                                                                                     gaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGc                                                  <  1:1741368/150‑1 (MQ=255)
                                                                                                      aaaCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCa                                                 >  1:1041367/1‑150 (MQ=255)
                                                                                                       aaCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                                                >  1:1451673/1‑150 (MQ=255)
                                                                                                       aaCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                                                >  1:1469320/1‑150 (MQ=255)
                                                                                                       aaCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGGTGAGAATGTTCGGCGCACCGTGTGCCAGGTCACCGTTCAGGTACACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGATACCCAg                                                >  2:319265/1‑150 (MQ=255)
                                                                                                           gCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCcg                                            >  2:60040/1‑150 (MQ=255)
                                                                                                             gAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCcgcg                                          >  2:1172987/1‑150 (MQ=255)
                                                                                                              aGGTCTTTCTGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCgcgc                                         <  1:960535/150‑1 (MQ=255)
                                                                                                                 tCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGa                                      <  1:149993/150‑1 (MQ=255)
                                                                                                                  cTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGAc                                     >  1:1206633/1‑150 (MQ=255)
                                                                                                                     tCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGtt                                  <  2:807520/150‑1 (MQ=255)
                                                                                                                      cAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTc                                 <  2:525582/150‑1 (MQ=255)
                                                                                                                      cAGCGCCATAATCAGCGACTCACCTTCAAAGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACAGTTCAGGTAAAATTCTTCGATATCTTTGATGCCGTTCCCCAGACGGTTACGCCGGCCGCGGCGACGTTc                                 >  1:285235/1‑150 (MQ=255)
                                                                                                                       aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTcc                                <  2:965911/150‑1 (MQ=255)
                                                                                                                         cgcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGAGGGTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAt                              <  1:106697/150‑1 (MQ=255)
                                                                                                                         cgcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAt                              <  1:941747/150‑1 (MQ=255)
                                                                                                                          gcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGCCGTTCCAtc                             >  1:507471/1‑150 (MQ=255)
                                                                                                                             aTAATCAGCGACTCACCTTCAACGTAGTTGACGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCAGCGCTGACGTTCCATCTCg                          >  1:266340/1‑150 (MQ=255)
                                                                                                                              tAATCCGCGACTCACCTTCACCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCgg                         <  1:1137953/150‑1 (MQ=255)
                                                                                                                                 tCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCg                      >  1:956369/1‑150 (MQ=255)
                                                                                                                                 tCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCg                      <  2:494716/150‑1 (MQ=255)
                                                                                                                                  cAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGCCGTTCCATCTCGGTCGc                     >  1:433069/1‑150 (MQ=255)
                                                                                                                                   tcaacgtCAGCTTCAACTACGTTGAAGGTGACGTTGAGAATGTTCGGCGCACCGTGATCCAGGTCACCCTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGCTTACGCAGGCCGCGCAGCCGTTCCATCTCGGTCGcc                    >  2:1483738/7‑150 (MQ=255)
                                                                                                                                    gCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCa                   >  1:1669669/1‑150 (MQ=255)
                                                                                                                                       cgtCAGCTTCAACTACGTTGAAGGTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCAtct                >  1:1053715/3‑150 (MQ=255)
                                                                                                                                       cgtCAGCTTCAACTACGTTGAAGGTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCAtcg                >  1:641750/3‑149 (MQ=255)
                                                                                                                                          cACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCtctt             >  1:917205/1‑150 (MQ=255)
                                                                                                                                            ccTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCtcttct           <  1:1276261/150‑1 (MQ=255)
                                                                                                                                             cTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTcttctt          >  1:42426/1‑150 (MQ=255)
                                                                                                                                             cTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTAAGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTcttctt          >  1:1619818/1‑150 (MQ=255)
                                                                                                                                              ttCAAGGTAGGTGAAGCTGACGTTCAGAATGTTCGGCTCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCATCGATATCTTTGATGCCGTTCCACAGTCGGTTACGCAGGCCGCGCAGACGGTCCATCTCGGTCGCCATCTCTTCttt         <  1:319307/150‑1 (MQ=255)
                                                                                                                                                cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtg       >  2:1001830/1‑150 (MQ=255)
                                                                                                                                                cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtg       >  1:1770726/1‑150 (MQ=255)
                                                                                                                                                 aaCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtgc      <  2:242714/150‑1 (MQ=255)
                                                                                                                                                 aaCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtgc      <  1:702004/150‑1 (MQ=255)
                                                                                                                                                  aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCTCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtgcg     <  2:223853/150‑1 (MQ=255)
                                                                                                                                                   cGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTtgcga    >  2:1813974/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTTGCGATG  >  NC_000913/2660463‑2660761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: