Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,753,986 A→T 8.0% intergenic (+40/+22) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,753,9860AT 8.0% 386.2 / 18.9 137intergenic (+40/+22)bamE/ratBlipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base A (61/65);  new base T (6/5);  total (67/70)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 138 total aligned reads displayed.

CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAA  >  NC_000913/2753838‑2754134
                                                                                                                                                    |                                                                                                                                                     
    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACACACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                                                                                                  >  1:898962/1‑150 (MQ=255)
    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                                                                                                  <  2:1559444/150‑1 (MQ=255)
    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                                                                                                  <  1:1368685/150‑1 (MQ=255)
    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                                                                                                  >  1:1537699/1‑150 (MQ=255)
       ccAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCAcc                                                                                                                                               >  2:424103/1‑150 (MQ=255)
       ccAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCAcc                                                                                                                                               <  1:1620490/150‑1 (MQ=255)
        cAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCt                                                                                                                                              <  1:570061/150‑1 (MQ=255)
          ggTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttt                                                                                                                                            <  2:909178/150‑1 (MQ=255)
           gTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttt                                                                                                                                           <  1:389294/150‑1 (MQ=255)
               tGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtct                                                                                                                                      >  2:1752681/1‑150 (MQ=255)
                gAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                                                                     <  1:1158336/150‑1 (MQ=255)
                gAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                                                                     <  1:232774/150‑1 (MQ=255)
                 aatgtgtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaacg                                                                                                                                     <  2:1624210/147‑5 (MQ=255)
                 aaGGTGTAACTCAGCAAACGCTGACGCTGACATTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaacg                                                                                                                                     <  2:351149/150‑5 (MQ=255)
                 aaGGTGTAACGCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATATCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTTCTCATTGAGCACCttttttaacg                                                                                                                                     <  2:495578/150‑5 (MQ=255)
                  aGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                                                                                                   <  2:658041/150‑1 (MQ=255)
                  aGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                                                                                                   <  1:864159/150‑1 (MQ=255)
                   ggTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtcg                                                                                                                                  >  1:1238198/1‑149 (MQ=255)
                    gtgtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtctg                                                                                                                                 >  2:825642/1‑150 (MQ=255)
                    gtgtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAAAAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtctg                                                                                                                                 <  1:1002805/150‑1 (MQ=255)
                        aaCTCAGAAAACGCTGACTATGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAAATAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttat                                                                                                                             <  1:1163308/150‑1 (MQ=255)
                             aGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGCGCACCGTTTTTCTGTCTGTtatttatt                                                                                                                        >  2:1088149/1‑150 (MQ=255)
                             aGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTtatttatt                                                                                                                        <  1:110931/150‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTACCCGTAGCGGTGTGTTGACCAATATTGATAACAACCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                                                                      <  2:1862333/150‑1 (MQ=255)
                                   cgctgacgctgaCCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGa                                                                                                                  >  1:1718397/1‑150 (MQ=255)
                                    gctgacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAt                                                                                                                 <  2:554913/150‑1 (MQ=255)
                                         cgctgccCTTTAACAGTTGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGTGTGGTAAGTAATAATAAAGTTGCTCTCATAGACGTTAAAAATGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                            <  1:781018/150‑1 (MQ=255)
                                         cgctgacCTTTAACAGTATCGGTGTGTTGACCAATATTGATAACAAACCTGCTCTGATTGGTAACTAATATTAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                            <  2:592005/150‑1 (MQ=255)
                                         cgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                            <  2:43355/150‑1 (MQ=255)
                                          gctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCt                                                                                                           >  2:936789/1‑150 (MQ=255)
                                            tgacCTTTAACAGTAGCTGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGc                                                                                                         <  2:16682/150‑1 (MQ=255)
                                            tgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATATCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGc                                                                                                         <  2:23135/150‑1 (MQ=255)
                                                cTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGt                                                                                                     >  1:429630/1‑150 (MQ=255)
                                                  ttAACCGTAGCGGTGTGTTGACCAATATTGCTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCGCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTg                                                                                                   <  2:304137/150‑1 (MQ=255)
                                                    aaCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                                                                                                 <  1:1302697/150‑1 (MQ=255)
                                                     aCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCt                                                                                                >  2:743529/1‑150 (MQ=255)
                                                      cAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTg                                                                                               >  2:231318/1‑150 (MQ=255)
                                                      cAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTg                                                                                               >  1:57715/1‑150 (MQ=255)
                                                            cGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGc                                                                                         <  2:1408460/150‑1 (MQ=255)
                                                                  gtTGACGATTATTGATACCAAACCTGCTCGGAGTGGTAACTTATAATAATGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                   <  1:327370/150‑1 (MQ=255)
                                                                  gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                   >  2:897964/1‑150 (MQ=255)
                                                                  gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                                   >  2:45762/1‑150 (MQ=255)
                                                                  gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCATGCGAAGCTCTTTc                                                                                   >  2:113759/1‑150 (MQ=255)
                                                                  gtTGACCAATATTGATAACAAACATGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCttta                                                                                   >  1:511669/1‑149 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCg                                                                                  <  2:1502262/150‑1 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGGGGTAACTAATAATACAGTTGCTCTCAAAGACGTTAAAAAATGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCg                                                                                  <  2:326263/150‑1 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                              <  2:668660/150‑1 (MQ=255)
                                                                        cAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTAAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCg                                                                             >  1:1199034/1‑150 (MQ=255)
                                                                          atatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAATTTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGc                                                                           <  1:274616/150‑1 (MQ=255)
                                                                                aTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgag                                                                     <  2:892149/150‑1 (MQ=255)
                                                                                aTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATAGGCAATgag                                                                     >  1:1217794/1‑150 (MQ=255)
                                                                                 tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                    >  2:1649347/1‑150 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                                                                   <  2:1537699/150‑1 (MQ=255)
                                                                                    cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAggg                                                                 >  2:873472/1‑150 (MQ=255)
                                                                                    cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAggg                                                                 <  1:743529/150‑1 (MQ=255)
                                                                                    cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAggg                                                                 >  1:461660/1‑150 (MQ=255)
                                                                                      aaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCg                                                               <  2:1374574/150‑1 (MQ=255)
                                                                                           gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAt                                                         <  1:386177/150‑1 (MQ=255)
                                                                                           gcgcTGAGGGGTCACTAATAATAAAGATGCGCTCAACGACCTTACAAAAGGTGCTCCATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATaaa                                                          <  2:1212867/150‑1 (MQ=255)
                                                                                             gcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc                                                        <  2:1789617/150‑1 (MQ=255)
                                                                                              cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtct                                                       >  1:1309518/1‑150 (MQ=255)
                                                                                                gAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc                                                     <  1:1511861/150‑1 (MQ=255)
                                                                                                gAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc                                                     <  1:1349403/150‑1 (MQ=255)
                                                                                                    ggTAACTAATAATAAGGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCCCCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGATCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAccc                                                 >  2:1247192/1‑150 (MQ=255)
                                                                                                    ggTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCAccc                                                 >  2:1587207/1‑150 (MQ=255)
                                                                                                      tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACATTTTTTCTGTATGTTATTTATTCGCTGATATTTCTGCTCTATGCATGCGAAGATCTATAGGATAGGCAATGAGATTGCGATAAATCTCAACCCGa                                               >  1:76958/1‑150 (MQ=255)
                                                                                                       aaCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGAt                                              <  2:1075967/150‑1 (MQ=255)
                                                                                                          taataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACATTTTTTCTGTGTGTTATTTATTGGCTGATTTTTCTGCTCGTTGCCTTCGAATCTCTTTAGGATCGGCAATGAGAGGGCGCTATATCTCCACCCGCTCGc                                           >  1:895075/1‑150 (MQ=255)
                                                                                                           aataataaAGCTGCGCTCTAAGACGTTAAAAAAGCTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGcc                                          <  2:146581/150‑1 (MQ=255)
                                                                                                            ataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCca                                         <  2:163284/150‑1 (MQ=255)
                                                                                                             taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcat                                        >  2:105710/1‑150 (MQ=255)
                                                                                                             taataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcat                                        <  1:925692/150‑1 (MQ=255)
                                                                                                             aaataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcat                                        <  2:500271/149‑1 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTTTTATTTATTCGCTGATTTTTATGCTCGTTGCCTGCGAAGCTATTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatc                                       >  2:804636/1‑150 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatc                                       >  2:1624284/1‑150 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatc                                       >  1:422892/1‑150 (MQ=255)
                                                                                                                taaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcatcat                                     >  1:1290634/1‑150 (MQ=255)
                                                                                                                 aaaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATg                                    >  1:1413750/1‑150 (MQ=255)
                                                                                                                  aaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGc                                   <  2:1330343/150‑1 (MQ=255)
                                                                                                                  aaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGc                                   >  2:550479/1‑150 (MQ=255)
                                                                                                                         tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTAt                            <  1:1752681/150‑1 (MQ=255)
                                                                                                                          ctcAAAGAAGTTAAAAAATGTGATCAATGAGCACCTTTTTTCTGTCTGTTATGTATTCGCGGATTTTTCTTCTAGTTGCCTGCGAAGCTCTTGCGGATCGGCAATGCGAGGGCCATAAATCTCCCCCCGATCGCCATCATGCCCCCTATc                           >  2:921911/1‑150 (MQ=255)
                                                                                                                           tcAAAGACGTTAACAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCg                          <  1:56225/150‑1 (MQ=255)
                                                                                                                            cAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGc                         >  2:788345/1‑150 (MQ=255)
                                                                                                                              aaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCtt                       >  1:1604289/1‑150 (MQ=255)
                                                                                                                                gACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAg                     <  1:1043043/150‑1 (MQ=255)
                                                                                                                                  cGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtt                   >  1:913466/1‑150 (MQ=255)
                                                                                                                                  acagaaaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCCCCCGATCGCCAGCCTGCACACAAGCGCTTAGtt                   >  2:886883/5‑150 (MQ=255)
                                                                                                                                     tAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTg                >  2:726336/1‑150 (MQ=255)
                                                                                                                                     tAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTg                >  2:1786217/1‑150 (MQ=255)
                                                                                                                                     tAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTg                >  1:666232/1‑150 (MQ=255)
                                                                                                                                      aaaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc               >  2:1168877/1‑150 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCATTGAGCACCTTTTTTAACGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc               >  1:152535/1‑150 (MQ=255)
                                                                                                                                        aaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGATGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCg             >  2:1276832/1‑150 (MQ=255)
                                                                                                                                            ggTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGAGAAGCTGTTTCGGATCGGCTATGAGCGGGCGATATATCTCCGCCCGATCGCCATCATGCACACGATCGGTTAGTTTTGCAGGACg         >  2:313044/1‑150 (MQ=255)
                                                                                                                                               tcTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGACGGACGGCt      <  1:1747956/149‑1 (MQ=255)
                                                                                                                                               gCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCt      >  2:963902/1‑150 (MQ=255)
                                                                                                                                                 gcAAGGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGt    <  1:324545/149‑1 (MQ=255)
                                                                                                                                                  cAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTa   >  1:185292/1‑150 (MQ=255)
                                                                                                                                                   aaTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTaa  >  2:540568/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCTGTAA  >  NC_000913/2753838‑2754134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: