Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,761,620 T→G 7.3% K641Q (AAG→CAG)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,761,6200TG 7.3% 345.3 / 14.0 118K641Q (AAG→CAG) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base T (58/51);  new base G (7/2);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 118 total aligned reads displayed.

GGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATA  >  NC_000913/2761474‑2761767
                                                                                                                                                  |                                                                                                                                                   
ggAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                                  >  1:999120/1‑150 (MQ=255)
 gAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa                                                                                                                                                 >  2:788239/1‑150 (MQ=255)
 gAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa                                                                                                                                                 >  2:913295/1‑150 (MQ=255)
  aaaaTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACCTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                                                                <  1:1718057/150‑1 (MQ=255)
  aaaaTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                                                                <  1:981762/150‑1 (MQ=255)
  aaaaTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                                                                >  1:1522922/1‑150 (MQ=255)
     aTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGCACGCCAATTTGACATGTGTGTAATCGACTTTGCTTCGTATACCACGGTTTACAGCgt                                                                                                                                             >  2:1132956/1‑150 (MQ=255)
     aTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                             >  1:663460/1‑150 (MQ=255)
        ccAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggt                                                                                                                                          <  2:505610/150‑1 (MQ=255)
         cAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtggtg                                                                                                                                         >  1:564703/1‑150 (MQ=255)
          aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                                                                        >  1:1053917/1‑150 (MQ=255)
          aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAAGGCCAATCTCTTCTAAGGCATTGACTCCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                                                                        <  1:217560/150‑1 (MQ=255)
           gCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGtt                                                                                                                                       <  1:1680301/150‑1 (MQ=255)
             aaGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatacg                                                                                                                                     <  1:878072/150‑6 (MQ=255)
                tCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACt                                                                                                                                  <  2:1593769/150‑1 (MQ=255)
                tCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACt                                                                                                                                  >  2:866509/1‑150 (MQ=255)
                   aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACa                                                                                                                               >  1:1663874/1‑150 (MQ=255)
                   aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACa                                                                                                                               <  1:1485254/150‑1 (MQ=255)
                    ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAt                                                                                                                              <  1:788239/150‑1 (MQ=255)
                       gTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTCCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcct                                                                                                                           >  2:772900/1‑150 (MQ=255)
                         gggTGTAAGGTCGGACTGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAACTTGACATGTGTGTAATGGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                         <  2:284717/149‑1 (MQ=255)
                         cGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                         >  1:1813284/1‑150 (MQ=255)
                         cGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                         <  2:756336/150‑1 (MQ=255)
                         cGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                         >  1:1255330/1‑150 (MQ=255)
                             gtAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCa                                                                                                                     >  1:1726196/1‑150 (MQ=255)
                             gtAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCa                                                                                                                     >  1:1389201/1‑150 (MQ=255)
                              tAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCaa                                                                                                                    <  2:1522910/150‑1 (MQ=255)
                               aaCGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAg                                                                                                                   <  1:1613892/150‑1 (MQ=255)
                               aaCGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAg                                                                                                                   <  2:1107382/150‑1 (MQ=255)
                                 cGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCt                                                                                                                 >  1:163148/1‑150 (MQ=255)
                                   tCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa                                                                                                               <  1:417561/150‑1 (MQ=255)
                                    cTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAg                                                                                                              >  2:1486541/1‑150 (MQ=255)
                                     ttaaTGGGAATGCCAATATCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGc                                                                                                             <  1:1190653/148‑1 (MQ=255)
                                      gAATGGGATTGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCa                                                                                                            <  1:1718109/150‑1 (MQ=255)
                                       aaTGGGAATGCCAATCTCTTCTAAGGCATTGACAGCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGTCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAc                                                                                                           <  1:1608759/150‑1 (MQ=255)
                                         tGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGATGCGTATACAACGCTTTACATCGTGTTGTTTAAATACATCCTCCATCAAGCTGAGCAcat                                                                                                         >  1:337836/1‑148 (MQ=255)
                                           ggAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                       <  2:374481/150‑1 (MQ=255)
                                             aaTGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtagg                                                                                                     <  1:781276/150‑1 (MQ=255)
                                               tGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                                   <  1:1193265/150‑1 (MQ=255)
                                                 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtatg                                                                                                 >  2:1382448/1‑148 (MQ=255)
                                                 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                                 >  2:1194344/1‑150 (MQ=255)
                                                 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                                 >  1:369050/1‑150 (MQ=255)
                                                 ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                                 >  2:1798373/1‑150 (MQ=255)
                                                        cttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGtatacc                                                                                          >  2:1516898/1‑148 (MQ=255)
                                                          tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTTATGGACTTTGCTGCGTATACCACGCTTTACCGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCg                                                                                        >  2:108954/1‑150 (MQ=255)
                                                           ctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGCGCACCTTAGGTAGGGTATAGCCGa                                                                                       >  1:262759/1‑150 (MQ=255)
                                                            tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCATTTTGACATGTGTGTAATCGACTGTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAGCCTCCATCAAGCTGCGCACCGTAGGTAGGGTATAGCCGaa                                                                                      >  1:1192503/1‑150 (MQ=255)
                                                              aGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAgg                                                                                    <  1:1830493/150‑1 (MQ=255)
                                                                 cATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAt                                                                                 >  1:563711/1‑150 (MQ=255)
                                                                    tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTgg                                                                              >  2:186241/1‑150 (MQ=255)
                                                                      acacCTGGGGGGAGATGGACCCTTTCGAACGCCAATTTGACATGTGTGTATTCGACTTTGCTGCGTATACCACGCTTTACAGCGGGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGtt                                                                            <  2:1162130/150‑1 (MQ=255)
                                                                        acCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt                                                                          <  2:1389201/150‑1 (MQ=255)
                                                                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTACCTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg                                                                         >  2:344363/1‑150 (MQ=255)
                                                                         ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg                                                                         <  2:793769/150‑1 (MQ=255)
                                                                            ggtggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGc                                                                      >  2:773791/1‑150 (MQ=255)
                                                                             gtggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCt                                                                     >  1:429010/1‑150 (MQ=255)
                                                                               ggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTc                                                                   <  2:564703/150‑1 (MQ=255)
                                                                                 tAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaa                                                                 <  2:559214/150‑1 (MQ=255)
                                                                                  aGATGGAAACTTTCGAACGACAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaaa                                                                <  2:1270016/150‑1 (MQ=255)
                                                                                   gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAAt                                                               <  2:705095/150‑1 (MQ=255)
                                                                                     tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTACCTACATCCTCCATCAAGCTGCGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCa                                                             >  2:853800/1‑150 (MQ=255)
                                                                                     tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATATCCGAAGGTATTTGTTATGAGCTTCAAATCa                                                             >  2:1539240/1‑150 (MQ=255)
                                                                                     tGGAAACTTTCGAACGCCAATTTGACATGTGGGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCTAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCa                                                             <  2:498236/150‑1 (MQ=255)
                                                                                       gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAAAGCGTGGGGTTTAACTAAATCCTCCATCAAGCTGACCACCTTAGGTAGGGTAAAGCCGAAGGTATAGGTTAGGACATTCAAATCAtt                                                           >  2:99581/1‑150 (MQ=255)
                                                                                              tCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGTCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAgg                                                    <  2:398259/150‑1 (MQ=255)
                                                                                                    gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGATGAGCACCTTAGGTATGTTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGTCAATg                                              >  1:1151973/1‑150 (MQ=255)
                                                                                                     gtaaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATgg                                             <  1:268184/148‑1 (MQ=255)
                                                                                                     ccAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAATCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATgg                                             <  1:913295/150‑1 (MQ=255)
                                                                                                       aaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaa                                           >  1:1386078/1‑150 (MQ=255)
                                                                                                         tttGACATGTGTGTAATCGACTTTGCTGGGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTAAAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAag                                         <  2:822576/150‑1 (MQ=255)
                                                                                                         tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAag                                         <  2:60660/150‑1 (MQ=255)
                                                                                                           tGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAagag                                       <  2:663460/150‑1 (MQ=255)
                                                                                                               aTGTGTGTACTCGACTTTGCTGCGTCTACCCCGCTTTACCGCGTGGTGTTTCACTCCATCCTCCATCAAGCTGAGCACCTTCGGGAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACg                                   <  2:230739/150‑1 (MQ=255)
                                                                                                               aTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACg                                   <  2:1726196/150‑1 (MQ=255)
                                                                                                                  tgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGCGCACCTTAGGTAGGGTATTGCCGAAGGTCTTGGTTTTGGGCGTCAAATCAGTGTTGAGTGCAATCGAAAGAGTAAGttt                                >  1:305212/1‑150 (MQ=255)
                                                                                                                         tCGACTTTGATGCGTAAACCAAGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGACCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCt                         <  2:347701/150‑1 (MQ=255)
                                                                                                                           gACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTc                       <  2:997358/150‑1 (MQ=255)
                                                                                                                                 gctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTTTGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAAt                 >  1:229028/1‑150 (MQ=255)
                                                                                                                                  ctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc                <  1:404550/150‑1 (MQ=255)
                                                                                                                                  ctgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc                >  1:523704/1‑150 (MQ=255)
                                                                                                                                   tgcGTAAACCACGCATTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTAAGGTAGGGTATAGCCGAAGGTATTGGGTATGCGCTGCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATcc               >  2:162626/1‑150 (MQ=255)
                                                                                                                                    gcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATccc              >  2:1002082/1‑149 (MQ=255)
                                                                                                                                     cGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAc             >  2:418283/1‑150 (MQ=255)
                                                                                                                                     cGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAc             <  2:659651/150‑1 (MQ=255)
                                                                                                                                      gTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACt            <  2:1773233/150‑1 (MQ=255)
                                                                                                                                       aacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTg           >  2:1809417/4‑150 (MQ=255)
                                                                                                                                       aacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTg           >  1:575682/4‑150 (MQ=255)
                                                                                                                                       aacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTg           >  2:725947/4‑150 (MQ=255)
                                                                                                                                        acaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAACTCCACTGt          >  2:486640/3‑150 (MQ=255)
                                                                                                                                        acaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGt          >  1:272074/3‑150 (MQ=255)
                                                                                                                                         taCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTc         >  2:1726417/1‑150 (MQ=255)
                                                                                                                                          aCCAGGCTTAACAGCTTGGTGTTTAACTACATCCTCCATCTACCTTAGCACCTTAGGTAGGGCATAGCAGAAGGCATTGGTTATGAGCTTCATAGCATTGTTGTCGGCAATGGAAAGAGTACGTTTCTCCCCTTCTGAAATCCACTGTCg        <  1:889444/150‑1 (MQ=255)
                                                                                                                                          aCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCg        >  1:1857860/1‑150 (MQ=255)
                                                                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGCGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt       >  2:1510747/1‑150 (MQ=255)
                                                                                                                                           ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt       >  2:1062093/1‑150 (MQ=255)
                                                                                                                                           ccACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt       >  1:1297292/1‑150 (MQ=255)
                                                                                                                                            cACGCTTTACAGCGTGGTGTTTACCTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTTTAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCGTTCAGAAATCCACTGTCGTg      >  2:511478/1‑150 (MQ=255)
                                                                                                                                            cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTg      <  2:1579259/150‑1 (MQ=255)
                                                                                                                                                cTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCTTGCATa  >  1:1084588/1‑150 (MQ=255)
                                                                                                                                                cTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATa  <  2:163148/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATA  >  NC_000913/2761474‑2761767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: