Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,531 G→T 10.5% intergenic (+70/‑284) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5310GT10.5% 241.8 / 6.9 95intergenic (+70/‑284)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (47/36);  new base T (2/10);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAT  >  NC_000913/2804383‑2804679
                                                                                                                                                    |                                                                                                                                                     
cGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGc                                                                                                                                                     >  1:1208618/1‑150 (MQ=255)
      cTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCAGCCAAATAGCTTTTTATCACGCAaataa                                                                                                                                               >  1:1232078/1‑150 (MQ=255)
       ttttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataat                                                                                                                                              >  1:95698/1‑150 (MQ=255)
       ttttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACAACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGAAaataat                                                                                                                                              >  1:1072692/1‑150 (MQ=255)
        tttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAtt                                                                                                                                             <  1:295977/150‑1 (MQ=255)
         ttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAttt                                                                                                                                            >  1:673579/1‑150 (MQ=255)
           actgTTCAGGCGCCTCTTCTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                          <  2:1168070/147‑1 (MQ=255)
             ggTTTATGCTACTCTTATGTGATGGGGAAATCTGGTGAAACATTAGAATAATACTTGAATTTCAGATGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                        <  1:1227419/150‑1 (MQ=255)
             ggTTCAGGCTGCTCTTTTGTGATGGGTAAAGCGGTTGTATCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACACGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCTCTCAAATAATTTgtgg                                                                                                                                        <  1:1060967/150‑1 (MQ=255)
             ggTTCAGGCTCCTCTTATGTGATGGGTAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAACTAGCGGTTTATCACGCAAATAATTTgtgg                                                                                                                                        <  1:1152279/150‑1 (MQ=255)
             ggTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                        <  1:1792663/150‑1 (MQ=255)
             ggTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                        <  1:403381/150‑1 (MQ=255)
             ggTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCAAAACCAGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTtgttg                                                                                                                                        >  1:746753/1‑148 (MQ=255)
             ggTCCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                        <  2:65684/150‑1 (MQ=255)
              gTTCATGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggt                                                                                                                                       <  2:1627961/150‑1 (MQ=255)
                 cagtttcctcTTAAGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                    <  2:1249926/145‑1 (MQ=255)
                  agtctcctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAATATATCTCTACACGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATc                                                                                                                                   <  2:510690/147‑1 (MQ=255)
                   ggCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCt                                                                                                                                  >  2:898868/1‑150 (MQ=255)
                   ggCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTAGTGGTGATCt                                                                                                                                  >  1:1572048/1‑150 (MQ=255)
                       cctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac                                                                                                                              <  1:602382/150‑1 (MQ=255)
                        ctctTATGTGTTGGGGAAAGCGTTTGAAAAAGAAGATGAAGACTGGAATTTCTGAGGGTTTTATTTTAAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                             <  1:138356/150‑1 (MQ=255)
                        ctctTATGTGATGGGGAAATAGGTTTAAACAGAAGATGAAGAATGGAATTTCTGAGGGTGTTATTTTCAAAATTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                             <  1:152381/150‑1 (MQ=255)
                          ctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTTGTGGAGATCTACACTGa                                                                                                                           >  2:898737/1‑150 (MQ=255)
                             aTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAc                                                                                                                        <  1:486289/150‑1 (MQ=255)
                               gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActc                                                                                                                      <  1:1623724/150‑1 (MQ=255)
                                tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActct                                                                                                                     >  2:1445712/1‑150 (MQ=255)
                                tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActct                                                                                                                     >  1:434539/1‑150 (MQ=255)
                                tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGAGATCTACACTGATActct                                                                                                                     >  1:166399/1‑150 (MQ=255)
                                  aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGt                                                                                                                   <  1:281720/150‑1 (MQ=255)
                                  aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTACAGAAATTTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCGGt                                                                                                                   <  2:646300/150‑1 (MQ=255)
                                    ggggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTg                                                                                                                 <  1:1026917/150‑1 (MQ=255)
                                     gggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGTGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTATTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGc                                                                                                                <  1:1516656/150‑1 (MQ=255)
                                     gggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGc                                                                                                                >  2:1204499/1‑150 (MQ=255)
                                      ggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGATTTTTATATGTTAATTATTTTGTGGTGATCTACACTGATACTCTGTTGCa                                                                                                               <  2:1838796/150‑1 (MQ=255)
                                        aaaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatt                                                                                                             <  1:898868/150‑1 (MQ=255)
                                        aaaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatt                                                                                                             >  2:1852067/1‑150 (MQ=255)
                                            cGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTc                                                                                                         <  1:1278329/150‑1 (MQ=255)
                                            cGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTc                                                                                                         <  1:1104255/150‑1 (MQ=255)
                                            cGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTc                                                                                                         <  1:573125/150‑1 (MQ=255)
                                              gTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTctc                                                                                                       >  1:753927/1‑148 (MQ=255)
                                               tttaaaCAGAAGATGAAGACTGGAATTTGTGAGGTTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTAGAGAATTTTTTTTTTTTTTATGTTAAGTAGTTTTTGGTGATCGACAGTGATACTCTGTTGCATGATTCggc                                                                                                      <  2:1183430/147‑3 (MQ=255)
                                                 gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTg                                                                                                    >  1:1494876/1‑150 (MQ=255)
                                                  aaaCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGa                                                                                                   >  2:1140624/1‑150 (MQ=255)
                                                   aaCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATTTCACTACCCGAAGCAGGTAAATAATTATATCTAATTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaa                                                                                                  <  2:216323/150‑1 (MQ=255)
                                                   aaCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaa                                                                                                  >  2:1213825/1‑150 (MQ=255)
                                                    aCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTACCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaaa                                                                                                 >  1:459284/1‑150 (MQ=255)
                                                       gaagaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACca                                                                                              >  2:251933/1‑150 (MQ=255)
                                                             gAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAatat                                                                                        >  2:519491/1‑150 (MQ=255)
                                                               aGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATATTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTc                                                                                      <  2:550378/150‑1 (MQ=255)
                                                                taCTGGAATTTGTGAGGGTGTTATTTTAAAAAATATCACTACCCGCAGCATTGAAATAATTCCCGCCAAATTTTTTTTTATAAGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCa                                                                                     <  2:713437/149‑1 (MQ=255)
                                                                gACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCa                                                                                     >  1:1768087/1‑150 (MQ=255)
                                                                  cTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                   >  1:1759135/1‑150 (MQ=255)
                                                                  cTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                   >  2:1650020/1‑150 (MQ=255)
                                                                  cTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                   >  1:1224136/1‑150 (MQ=255)
                                                                   tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGc                                                                                  >  2:775893/1‑150 (MQ=255)
                                                                   tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGc                                                                                  >  2:711794/1‑150 (MQ=255)
                                                                    gtaaTTTCTGATCTTTTTATTTTCAAAAATATCACTACCCGCAGCAGGTATATAATTCCCGCCAAATATCTTTTTATCACGCAAATAATTTGTGCTGATCTACACTGATACTCTGTTGCAGTATTCGCCTGAAACCACAATAATCAGGCg                                                                                 <  1:132342/148‑1 (MQ=255)
                                                                    ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTATATCACGCAAATAATATGTGGCGATCTACACTGATACTCTGTGGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                                 <  1:898737/150‑1 (MQ=255)
                                                                        tttCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACAACAATATTCAGGCGattt                                                                             >  2:509158/1‑150 (MQ=255)
                                                                           cTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTc                                                                          <  1:1445712/150‑1 (MQ=255)
                                                                           cTGAGGGTGTTATTTTCAAAAATATCACTAACCGCAGCAGGGAAATAATTACCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCATGAAACCACAAAATTCATGCGtttttta                                                                          >  2:1346213/1‑149 (MQ=255)
                                                                            tGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCg                                                                         >  1:467584/1‑150 (MQ=255)
                                                                               gggTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTa                                                                      <  1:1442645/150‑1 (MQ=255)
                                                                                   gtTATATTCAAAAATATCACTACCCTCAGCATGGAAATAATTCCCGCCAAATAGTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                                  <  2:1503386/150‑1 (MQ=255)
                                                                                     tATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTACCTCCAAATATTTTTTTTTATGTTAATTATTTTTTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTg                                                                <  2:1711113/150‑1 (MQ=255)
                                                                                      aTTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAATTAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGa                                                               >  1:139511/1‑150 (MQ=255)
                                                                                      aTTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGa                                                               >  2:1566979/1‑150 (MQ=255)
                                                                                       ttttCAAAAATATCACTACCCGCAGAAGGGAAATAATTCCCGCCAAATAGATTTTTATCACTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                              <  2:640742/150‑1 (MQ=255)
                                                                                           cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                          >  1:977118/1‑150 (MQ=255)
                                                                                           cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                          >  1:382412/1‑150 (MQ=255)
                                                                                           cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCACCTGAAACAACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                          >  2:1054802/1‑150 (MQ=255)
                                                                                                atatCACTACCCGCAGCAGGGAAATAATTACCGCCAATTAGCTTTTTATCACTCAAATAATTTGAGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCATGCGTTTTTTCGCTATCTTTGACAAAAAatat                                                     <  1:262193/150‑1 (MQ=255)
                                                                                                  atCACTACCCGCAGCAGGGAAATAATTCCCTCCAATTTTTTTTTGTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATGATGCGCCTGAAACCACGAGATTCAGGCGTTTTGGCGCTATCTTGGACAAAAAAGATCa                                                   <  2:1072692/150‑1 (MQ=255)
                                                                                                   tCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCaa                                                  >  1:1247669/1‑150 (MQ=255)
                                                                                                    cACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAAc                                                 <  1:709069/150‑1 (MQ=255)
                                                                                                     aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGAAAACTTTGACAAAAAATATCAACt                                                >  2:993413/1‑150 (MQ=255)
                                                                                                      atACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACtt                                               <  1:652138/149‑1 (MQ=255)
                                                                                                        aCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtc                                             <  1:1582088/150‑1 (MQ=255)
                                                                                                        aCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtc                                             <  1:904178/150‑1 (MQ=255)
                                                                                                             cagcagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGAt                                        >  2:578657/1‑150 (MQ=255)
                                                                                                                cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGCAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTg                                     >  2:462724/1‑150 (MQ=255)
                                                                                                                         aatTCCCGCCAAATAGATTTTTATAACTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGcc                            <  2:1810608/150‑1 (MQ=255)
                                                                                                                          aatCCCGCCAAATAGGTTTTTATCTCGTAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCACTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGcct                           <  2:1224136/148‑2 (MQ=255)
                                                                                                                            tCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAGCCAAAATATTCAGGCGTTTTTTCGCTATCTTTGAAAAAAAATATCAACTTTCTCGATTTGCTCACAGCCCtt                         >  2:742767/1‑150 (MQ=255)
                                                                                                                              ccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCATtat                       >  2:1607163/1‑150 (MQ=255)
                                                                                                                               cGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTtata                      <  1:519491/150‑1 (MQ=255)
                                                                                                                                 ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTATTTTCCCTAACTTTCACAAAAAATATCAACTTTCTCGATTTTCACTAAGCCCTTATATc                    >  2:1056913/1‑150 (MQ=255)
                                                                                                                                  cAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCTCAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCa                   <  1:163823/150‑1 (MQ=255)
                                                                                                                                   aaaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCAc                  >  1:1624524/1‑150 (MQ=255)
                                                                                                                                       aGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGa              >  2:722698/1‑150 (MQ=255)
                                                                                                                                       aGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACAACAATATTCAGGCGAATAATCCATAACATTAACAAAAAATATCAACTTTCTCGAATTAAACTAAACCATAATATAACGGGa              >  2:978952/1‑150 (MQ=255)
                                                                                                                                                 cACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGCAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCg    >  1:1274052/1‑150 (MQ=255)
                                                                                                                                                 cACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCg    >  1:1266815/1‑150 (MQ=255)
                                                                                                                                                  aCGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGa   >  1:1467035/1‑150 (MQ=255)
                                                                                                                                                   cGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAt  >  2:900916/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAT  >  NC_000913/2804383‑2804679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: