Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,854,313 T→A 5.1% intergenic (+49/‑25) hypE → / → fhlA carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein/formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,854,3130TA 5.1% 462.9 / 9.1 151intergenic (+49/‑25)hypE/fhlAcarbamoyl dehydratase, hydrogenases 1,2,3 maturation protein/formate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (76/67);  new base A (4/4);  total (80/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

GTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGC  >  NC_000913/2854164‑2854462
                                                                                                                                                     |                                                                                                                                                     
  taaGTGGTTGAACGTAAATGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAATTTCTTAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGc                                                                                                                                                     <  2:1641933/149‑1 (MQ=255)
    aGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCgg                                                                                                                                                   >  2:1789643/1‑150 (MQ=255)
        gtggAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCt                                                                                                                                               >  1:1800410/1‑150 (MQ=255)
          ggAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGTTTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCttt                                                                                                                                             >  2:1163063/1‑150 (MQ=255)
          ggAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCCCACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCttt                                                                                                                                             >  2:1680403/1‑150 (MQ=255)
          ggAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCttt                                                                                                                                             >  1:800548/1‑150 (MQ=255)
           gAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCAAAGCGGTGcaaaa                                                                                                                                            <  1:1607236/150‑5 (MQ=255)
           gAACGTAAAGGTGTTCGTCTTGACGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCAAAGCGGTGcaaaa                                                                                                                                            <  1:164057/150‑5 (MQ=255)
            aaCGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCAAAGCGGTGcaaaag                                                                                                                                           <  1:1282586/150‑6 (MQ=255)
            aaCGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGAGTGAAACGAACCCTCGAGTTACCACACTCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATCGTTAGTCAATGACCTTTTGCACCGCAAAGCGGTGcaaaag                                                                                                                                           <  1:1502679/150‑6 (MQ=255)
                 aaaGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGgaa                                                                                                                                      <  1:768243/150‑1 (MQ=255)
                  aaGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGgaag                                                                                                                                     <  2:708396/150‑1 (MQ=255)
                    ggTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGgaagaa                                                                                                                                   >  1:371707/1‑150 (MQ=255)
                      tgtTCGTCTTGCCGATCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACa                                                                                                                                 >  1:1144057/1‑150 (MQ=255)
                           gTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATg                                                                                                                            <  1:198181/150‑1 (MQ=255)
                             cTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTc                                                                                                                          >  1:600261/1‑150 (MQ=255)
                               tGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCat                                                                                                                        <  1:1419967/150‑1 (MQ=255)
                                gCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCata                                                                                                                       >  1:1253286/1‑150 (MQ=255)
                                gCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGGTTATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAAGGACCTTTTGCACCGCTTTGCGGTGGGTGCCTGGAAGAACAAAATGTCatg                                                                                                                       >  2:1221702/1‑149 (MQ=255)
                                   ggTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATac                                                                                                                    >  2:1241386/1‑150 (MQ=255)
                                   ggTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATac                                                                                                                    >  1:1622443/1‑150 (MQ=255)
                                   ggTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATac                                                                                                                    >  1:1312169/1‑150 (MQ=255)
                                      cTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACAcc                                                                                                                 <  2:1220191/150‑1 (MQ=255)
                                      cTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACAcc                                                                                                                 >  2:1390636/1‑150 (MQ=255)
                                      cTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACAcc                                                                                                                 <  2:865863/150‑1 (MQ=255)
                                       tGTATGGCGTGAAACGAACCCACGATATACCACACGCCGAACCGCTTACGCGTATATGATAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTTCTTTCCTGGAAGAACAAAATTTCATATACACCg                                                                                                                >  1:85364/1‑150 (MQ=255)
                                          aTGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATg                                                                                                             >  2:1016045/1‑150 (MQ=255)
                                            ggCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAg                                                                                                           >  2:1853619/1‑150 (MQ=255)
                                              cGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTg                                                                                                         <  2:821239/150‑1 (MQ=255)
                                               gTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGa                                                                                                        >  1:805833/1‑150 (MQ=255)
                                                  aaaCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGAtct                                                                                                     <  1:315601/150‑1 (MQ=255)
                                                   aaCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGAtctc                                                                                                    >  1:1011271/1‑150 (MQ=255)
                                                    aCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                                                                   >  2:503543/1‑150 (MQ=255)
                                                    aCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                                                                   >  2:128850/1‑150 (MQ=255)
                                                    aCGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCg                                                                                                   >  1:1482445/1‑150 (MQ=255)
                                                     cGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCgg                                                                                                  <  2:648665/150‑1 (MQ=255)
                                                      gAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGa                                                                                                 <  2:1511888/150‑1 (MQ=255)
                                                       aaCCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGac                                                                                                <  1:683987/150‑1 (MQ=255)
                                                       aaCCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGac                                                                                                <  2:554078/150‑1 (MQ=255)
                                                          ccTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGacaac                                                                                             >  1:1354788/1‑150 (MQ=255)
                                                           cTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGacaaca                                                                                            <  1:863902/150‑1 (MQ=255)
                                                              gATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAgg                                                                                         <  2:671029/150‑1 (MQ=255)
                                                                     cacaCGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTc                                                                                  >  2:1139451/1‑150 (MQ=255)
                                                                      acacGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTCTAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCg                                                                                 <  1:317382/150‑1 (MQ=255)
                                                                      acacGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCg                                                                                 <  2:895094/150‑1 (MQ=255)
                                                                         cGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACa                                                                              >  1:516595/1‑150 (MQ=255)
                                                                         cGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACa                                                                              >  1:175507/1‑150 (MQ=255)
                                                                           ccGAACCGCTTCCGCGTATATGCTAATAACAGTCTAAGTCTGCTATAGTTAGTCAAGGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACTACAAGGGTTGTTCGACATc                                                                            >  2:287114/1‑150 (MQ=255)
                                                                             gacccGCTTGCTCGTATATGCTAATATAATTCTAACTCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTCCGGTGCTTTCCTGGAATAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCAc                                                                          <  2:76234/147‑1 (MQ=255)
                                                                               aCCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTc                                                                        >  1:141945/1‑150 (MQ=255)
                                                                                ccGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGCCCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCg                                                                       >  1:1813733/1‑150 (MQ=255)
                                                                                    ttCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATGTCGAACAACCCTTGTTGTCCGAGATCACTcatcgg                                                                   <  1:461888/150‑6 (MQ=37)
                                                                                     tCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATGTCGAACAACCCTTGTTGTCCGAGATCACTcatcggt                                                                  <  1:281091/150‑7 (MQ=37)
                                                                                     tCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGacac                                                                  >  1:1755136/1‑150 (MQ=255)
                                                                                      ccGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACt                                                                 >  1:1386335/1‑150 (MQ=255)
                                                                                        gcgTATATTCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTAt                                                               <  2:1242272/150‑1 (MQ=255)
                                                                                        gcgTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTAt                                                               >  1:928426/1‑150 (MQ=255)
                                                                                                gCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCCCCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATCTACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGcagcag                                                       <  1:372495/150‑1 (MQ=255)
                                                                                                gCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGcagcag                                                       <  2:1330341/150‑1 (MQ=255)
                                                                                                  aaataaAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCCCCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCAAATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGcc                                                     <  2:267224/149‑1 (MQ=255)
                                                                                                    ataaAATTCTAAATCTACTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCAGATCCACCGATGACTGATCTCGGACAACTACGGTGGTTCGACAGCCGTCGGACACTATTGAagaagcagg                                                   >  2:310821/1‑147 (MQ=255)
                                                                                                     taaAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTTGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGa                                                  >  1:922841/1‑150 (MQ=255)
                                                                                                     taaAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGa                                                  >  2:880651/1‑150 (MQ=255)
                                                                                                     taaAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGa                                                  >  2:1815879/1‑150 (MQ=255)
                                                                                                       aaaTTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATc                                                <  2:175507/150‑1 (MQ=255)
                                                                                                        aaTTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCt                                               >  2:193864/1‑150 (MQ=255)
                                                                                                        aaTTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCt                                               <  1:1789643/150‑1 (MQ=255)
                                                                                                           tCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGc                                            <  1:1163063/150‑1 (MQ=255)
                                                                                                             tAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCt                                          >  2:104539/1‑150 (MQ=255)
                                                                                                               aaTCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTACTGGAAGAACAAAAAGTCATATACACCGATCAGTGATCTCGTACATCAAGGGTTGTTCGACAACACTCGGAAACAATTACATCAGCCCGATCTGTCATCg                                        >  1:1364434/1‑150 (MQ=255)
                                                                                                               aaTCTCCTAAAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCTGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCg                                        <  2:350075/150‑1 (MQ=255)
                                                                                                                aTCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGc                                       >  2:1042986/1‑150 (MQ=255)
                                                                                                                 tctcCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCt                                      <  2:764636/150‑1 (MQ=255)
                                                                                                                 tctcCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCt                                      <  1:1397158/150‑1 (MQ=255)
                                                                                                                  ctcCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCtg                                     >  2:805769/1‑150 (MQ=255)
                                                                                                                    ccTATAGTTAGTCATTGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGCGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCAGATCTGGCCTCGCtgtg                                   >  2:715043/1‑150 (MQ=255)
                                                                                                                    ccTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAATAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGTGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCtggg                                   >  1:1008842/1‑148 (MQ=255)
                                                                                                                    ccTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCtgtg                                   <  2:1011271/150‑1 (MQ=255)
                                                                                                                    ccTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGCCCTCGCtgtg                                   >  1:830590/1‑150 (MQ=255)
                                                                                                                     cTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCtgtgt                                  <  2:1101812/150‑1 (MQ=255)
                                                                                                                      tttagttagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCtgtgtg                                 <  2:454444/148‑1 (MQ=255)
                                                                                                                      tttagtgagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGCACAAAATGTAATATACACCGATGCGTGATCTCGGACAACAAGGGTTGTCCGACATCACTCGGACACTAGTGCAGCAGCCCGATCTGGCCTCGCggtgtg                                 <  2:263512/148‑1 (MQ=255)
                                                                                                                        tagttagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACACGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAg                               <  2:1431086/150‑1 (MQ=255)
                                                                                                                          gttagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGc                             <  2:804922/150‑1 (MQ=255)
                                                                                                                          gttagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGc                             >  2:802900/1‑150 (MQ=255)
                                                                                                                          gttagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGc                             <  1:398229/150‑1 (MQ=255)
                                                                                                                            tagtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGctc                           >  2:604692/1‑150 (MQ=255)
                                                                                                                             agtCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGctct                          >  2:198990/1‑150 (MQ=255)
                                                                                                                                   tGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCa                    <  2:692541/150‑1 (MQ=255)
                                                                                                                                     caggaaagCACCGCAAAGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGATCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAAc                  >  2:819788/8‑150 (MQ=255)
                                                                                                                                      ccTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACt                 >  2:1562763/1‑150 (MQ=255)
                                                                                                                                      aggaaagCACCGCAAAGCGGTGCTTTACTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACt                 >  2:1744636/7‑150 (MQ=255)
                                                                                                                                        ttttGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTgg               <  2:800548/150‑1 (MQ=255)
                                                                                                                                         aaagCACCGCAAAGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGt              >  1:1264523/4‑150 (MQ=255)
                                                                                                                                            gCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTaaa           >  2:1294369/1‑150 (MQ=255)
                                                                                                                                             cACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAg          >  1:1836941/1‑150 (MQ=255)
                                                                                                                                             cACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAg          <  2:1383148/150‑1 (MQ=255)
                                                                                                                                               ccGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCg        <  2:3403/150‑1 (MQ=255)
                                                                                                                                               ccGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCg        >  2:397188/1‑150 (MQ=255)
                                                                                                                                                     tGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGC  <  2:404873‑M1/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGGTGCTTTCCTGGAAGAACAAAATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCTGGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGC  >  NC_000913/2854164‑2854462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: