Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,919,905 G→C 5.0% T281S (ACC→AGC)  gudX ← glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,919,9050GC 5.0% 388.6 / 9.9 119T281S (ACC→AGC) gudXglucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base G (52/61);  new base C (3/3);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 121 total aligned reads displayed.

GTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGT  >  NC_000913/2919757‑2920053
                                                                                                                                                    |                                                                                                                                                    
gtcgtcGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTTCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCCAGCCggg                                                                                                                                                     >  2:74109/1‑149 (MQ=255)
  cgtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCGAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCg                                                                                                                                                   <  1:1597553/150‑1 (MQ=255)
  cgtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCg                                                                                                                                                   <  1:946068/150‑1 (MQ=255)
   gtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGc                                                                                                                                                  <  1:753967/150‑1 (MQ=255)
    tcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGGCGCGACGGGCACGCCGGTCGcc                                                                                                                                                 >  2:812663/1‑150 (MQ=255)
     cgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGATTATCTACCGCATTGAGCATCCCCGCATGACACATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGccc                                                                                                                                                >  2:809911/1‑150 (MQ=255)
      gCAAATCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                               <  1:1452257/150‑1 (MQ=255)
         aaGCTGCGCCACACGGACTGCAGCGGAAAGCGGCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCAGGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCg                                                                                                                                            <  1:722736/150‑1 (MQ=255)
           gCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcg                                                                                                                                          <  2:1830599/150‑3 (MQ=255)
                gcCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcgac                                                                                                                                        <  1:986912/147‑5 (MQ=255)
                  cacaCGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGc                                                                                                                                   <  2:1697549/150‑1 (MQ=255)
                   acacGGACTGCTCCGGACAGCGGCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGCCCCATATCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGcc                                                                                                                                  <  2:1825925/150‑1 (MQ=255)
                        gACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCAc                                                                                                                             <  2:1179522/150‑1 (MQ=255)
                          cTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACtt                                                                                                                           >  1:892522/1‑150 (MQ=255)
                            gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                         <  2:1079497/150‑1 (MQ=255)
                                cGGAAAGCCTCCAGAAGTGCGGATCGGCAAGTGGAAAATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTc                                                                                                                     <  2:1673501/150‑1 (MQ=255)
                                 ggAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAACCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTcc                                                                                                                    >  2:323814/1‑150 (MQ=255)
                                   acaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                  <  2:1759879/148‑1 (MQ=255)
                                   aaaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATGGCGCAACACCGCATGACCCATTTCGCGCCAGAGGGGGGCGATCATGTTACTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                  <  2:114418/150‑1 (MQ=255)
                                        gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAg                                                                                                             >  1:1634086/1‑150 (MQ=255)
                                        gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAg                                                                                                             >  1:1574300/1‑150 (MQ=255)
                                           cAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGAGCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCAGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGccc                                                                                                          <  1:1226502/150‑1 (MQ=255)
                                             gAAGTGCGGATCAGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGTTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                        <  2:1459848/150‑1 (MQ=255)
                                                gTGCGGATCGGCGAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                     <  1:78921/150‑1 (MQ=255)
                                                gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGTCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                     >  2:1031628/1‑150 (MQ=255)
                                                gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                     <  1:596219/150‑1 (MQ=255)
                                                gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                     >  1:538703/1‑150 (MQ=255)
                                                 tGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCTCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCTTCACTTCACGTCCGGAGAAGCCCTGTTCt                                                                                                    <  2:1193763/150‑1 (MQ=255)
                                                  gCGGAGCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTg                                                                                                   <  2:647060/150‑1 (MQ=255)
                                                    ggATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcg                                                                                                 >  1:443060/1‑150 (MQ=255)
                                                      aTCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGcgcc                                                                                               >  1:825938/1‑150 (MQ=255)
                                                        cGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGcgccgc                                                                                             >  1:1076323/1‑150 (MQ=255)
                                                         ggCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGGTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCa                                                                                            >  1:1759647/1‑150 (MQ=255)
                                                          gCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTAAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCAt                                                                                           <  1:34718/150‑1 (MQ=255)
                                                            aaGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATgg                                                                                         <  2:524378/150‑1 (MQ=255)
                                                            aaGGGGAATATCTACCGGATTGAGCATAACCGCATGACCCATTTCGCGCCAGTTGTGGGCTATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCTTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTTTTATGCGCCGCATgg                                                                                         <  1:759958/150‑1 (MQ=255)
                                                              gTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGGCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAt                                                                                       <  2:1586761/150‑1 (MQ=255)
                                                              gTGCAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTGGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCTAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAt                                                                                       <  2:49966/150‑1 (MQ=255)
                                                               tGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATc                                                                                      <  1:543249/150‑1 (MQ=255)
                                                                ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGGAGGAGCCCTGTTCTGCGCCGCATGGATcg                                                                                     >  1:165691/1‑149 (MQ=255)
                                                                ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                     >  1:130485/1‑150 (MQ=255)
                                                                ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                     >  2:1441448/1‑150 (MQ=255)
                                                                  aaTATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAGCCCTGTTCTGCGCCGCATGGATCTTc                                                                                   >  1:934853/1‑150 (MQ=255)
                                                                     atCTACCGCATTGAGCATCACCGCATGACGCATTTCGCGCCAGTTGTTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                <  1:1683805/150‑1 (MQ=255)
                                                                     atCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                >  2:460085/1‑150 (MQ=255)
                                                                     atCTACCGCATTGAGCATCACCGCATGACCCAGTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                <  2:920245/150‑1 (MQ=255)
                                                                      tCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCa                                                                               <  1:1441448/150‑1 (MQ=255)
                                                                      tCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCa                                                                               <  1:723199/150‑1 (MQ=255)
                                                                       cTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAt                                                                              >  1:1581417/1‑150 (MQ=255)
                                                                        tACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATa                                                                             >  2:1595647/1‑150 (MQ=255)
                                                                         aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                            >  1:1350352/1‑150 (MQ=255)
                                                                         aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                            <  2:1837755/150‑1 (MQ=255)
                                                                          ccGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAgg                                                                           <  1:1517873/150‑1 (MQ=255)
                                                                            gCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCTGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTa                                                                         <  1:888276/150‑1 (MQ=255)
                                                                              aTTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGCTCTTCGGCATAGGTAAg                                                                       >  1:919031/1‑150 (MQ=255)
                                                                               ttGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGCTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGGCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGa                                                                      <  1:1126027/150‑1 (MQ=255)
                                                                                tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATATGTAAGaa                                                                     <  2:1758037/150‑1 (MQ=255)
                                                                                tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGaa                                                                     <  2:919442/150‑1 (MQ=255)
                                                                                 gAGCATCCCCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGGTTGTCGCGACGGGCAAGCCCGTCGCCCGTCGAATTACCGCCATCACTTCACGTCCGGAGAAGCGCTGGTCTGCGCCGCATGGATCTTCGGCTTAggacagagc                                                                    >  2:331203/1‑143 (MQ=255)
                                                                                 gAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGGCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAc                                                                    >  2:73206/1‑150 (MQ=255)
                                                                                  aGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAca                                                                   <  1:1590219/150‑1 (MQ=255)
                                                                                   gCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcat                                                                  >  1:1791928/1‑150 (MQ=255)
                                                                                    cATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatc                                                                 >  1:588541/1‑150 (MQ=255)
                                                                                     aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                <  1:997995/150‑1 (MQ=255)
                                                                                      tCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatcat                                                               <  2:842800/150‑1 (MQ=255)
                                                                                      tCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatcat                                                               <  2:693679/150‑1 (MQ=255)
                                                                                        aCCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTACGCCATCACTTCACGTCCGGTGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTc                                                             >  1:910333/1‑150 (MQ=255)
                                                                                          cGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAg                                                           <  2:473122/150‑1 (MQ=255)
                                                                                               gACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCtt                                                      >  1:1862645/1‑150 (MQ=255)
                                                                                                 cccATTTCTCTCCATTTTTTGGCGATCATGTTAGTCTCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGGCATCACTTCATGTCCGGAGAAGCCCTGTGCTGCGCCGGATGGATCTTCTGCATAGGTAAGAACATCATTCTGCCCtttt                                                    <  2:1756843/150‑2 (MQ=255)
                                                                                                   cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                  >  2:1007975/1‑150 (MQ=255)
                                                                                                   cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                  <  1:1451309/150‑1 (MQ=255)
                                                                                                    aTTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCAt                                                 <  2:1076323/150‑1 (MQ=255)
                                                                                                       tCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCTCATGGATCTTCGGCATAGGTAAGAACATCAATCAGCCCTTTGCATaaa                                              <  1:92153/150‑1 (MQ=255)
                                                                                                        cgcgCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAg                                             <  2:1602590/150‑1 (MQ=255)
                                                                                                         gcgcCAGTTGGGGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                            <  1:276296/150‑1 (MQ=255)
                                                                                                               gTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAGGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGc                                      >  1:1772116/1‑150 (MQ=255)
                                                                                                                  ggtggCGATCTTGTTAGTCGCGACGGGCAAGCCGGTCGACCGTCGAAATTCCGCCATCACTTCGCGTCCGGAGAAGCCATGGTATGCGACGCATGGATCTTCGGCCGAGGTAAGAACATCATTCAGCCAGTTGCATAAAGAAATGGCTtc                                   >  2:159351/1‑150 (MQ=255)
                                                                                                                  ggtggCGATCATGGTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGTAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtc                                   <  2:133298/150‑1 (MQ=255)
                                                                                                                       cGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCaa                              >  1:856453/1‑150 (MQ=255)
                                                                                                                        gATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAag                             >  1:406171/1‑150 (MQ=255)
                                                                                                                         aTCAGGGTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGATCAGCATGGATCTTCGGCATAGGTAAGAACATCATTCATCCCTTTGCATAAAGAAATGGCTTCATCAagc                            <  1:107384/150‑1 (MQ=255)
                                                                                                                           cATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                          <  2:1799544/150‑1 (MQ=255)
                                                                                                                            aTGTTAGTCGCGACGGGCAAGCCGGGCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcagc                         <  2:1235231/150‑1 (MQ=255)
                                                                                                                             tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                        >  2:982242/1‑150 (MQ=255)
                                                                                                                             tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                        >  1:1036158/1‑150 (MQ=255)
                                                                                                                             tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCAAGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                        >  2:1474657/1‑150 (MQ=255)
                                                                                                                              gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGCTCTTCGGCATAGGGAAGAACCTCCTTCCGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                       >  1:178154/1‑150 (MQ=255)
                                                                                                                               ttAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTCCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCAt                      <  2:1112981/150‑1 (MQ=255)
                                                                                                                                  gTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGGAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTAATCAAGCAGCCATGCa                   >  2:264056/1‑150 (MQ=255)
                                                                                                                                    ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                 >  1:1564161/3‑150 (MQ=255)
                                                                                                                                    cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACAACATTCAGCCCTTTGCATAAAGAAATGGCGTCATCAAGCAGCCATGCAcc                 >  2:1401346/1‑150 (MQ=255)
                                                                                                                                    cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                 >  2:620738/1‑150 (MQ=255)
                                                                                                                                    cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                 >  1:1848058/1‑150 (MQ=255)
                                                                                                                                       gACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGtt              >  2:1398343/1‑150 (MQ=255)
                                                                                                                                            gCAAGCCGGGCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGAt         <  2:482917/150‑1 (MQ=255)
                                                                                                                                             cAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCTAGCAGCCATGCACCGTTGGGATc        >  1:289219/1‑150 (MQ=255)
                                                                                                                                               aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCTTAGGTAAGAAGATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa      >  2:153652/1‑150 (MQ=255)
                                                                                                                                                 ccGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATATAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACg    >  1:807522/1‑150 (MQ=255)
                                                                                                                                                   ggTCGCCCGTCGAAATTCCGCCATCACTTCACGGCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGTCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGt  <  1:169133/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGT  >  NC_000913/2919757‑2920053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑