Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,919,907 C→T 5.0% A280A (GCG→GCA)  gudX ← glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,919,9070CT 5.0% 389.9 / 5.6 119A280A (GCG→GCA) gudXglucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base C (51/62);  new base T (3/3);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 120 total aligned reads displayed.

CGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAA  >  NC_000913/2919759‑2920055
                                                                                                                                                    |                                                                                                                                                    
cgtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCGAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCg                                                                                                                                                     <  1:1597553/150‑1 (MQ=255)
 gtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGc                                                                                                                                                    <  1:753967/150‑1 (MQ=255)
   cgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGATTATCTACCGCATTGAGCATCCCCGCATGACACATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGccc                                                                                                                                                  >  2:809911/1‑150 (MQ=255)
   cgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGccc                                                                                                                                                  <  1:384842/150‑1 (MQ=255)
    gCAAATCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                 <  1:1452257/150‑1 (MQ=255)
       aaGCTGCGCCACACGGACTGCAGCGGAAAGCGGCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCAGGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCg                                                                                                                                              <  1:722736/150‑1 (MQ=255)
              gcCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcgac                                                                                                                                          <  1:986912/147‑5 (MQ=255)
                cacaCGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGc                                                                                                                                     <  2:1697549/150‑1 (MQ=255)
                 acacGGACTGCTCCGGACAGCGGCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGCCCCATATCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGcc                                                                                                                                    <  2:1825925/150‑1 (MQ=255)
                      gACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCAc                                                                                                                               <  2:1179522/150‑1 (MQ=255)
                         tGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTc                                                                                                                            <  2:474804/150‑1 (MQ=255)
                          gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCTCCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTGAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                           >  2:1169051/1‑150 (MQ=255)
                              cGGAAAGCCTCCAGAAGTGCGGATCGGCAAGTGGAAAATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTc                                                                                                                       <  2:1673501/150‑1 (MQ=255)
                               ggAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAACCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTcc                                                                                                                      >  2:323814/1‑150 (MQ=255)
                                 acaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                    <  2:1759879/148‑1 (MQ=255)
                                 aaaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATGGCGCAACACCGCATGACCCATTTCGCGCCAGAGGGGGGCGATCATGTTACTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                    <  2:114418/150‑1 (MQ=255)
                                      gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAg                                                                                                               >  1:1634086/1‑150 (MQ=255)
                                      gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAg                                                                                                               >  1:1574300/1‑150 (MQ=255)
                                        ccAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGcc                                                                                                             <  2:1662691/150‑1 (MQ=255)
                                         cAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGAGCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCAGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGccc                                                                                                            <  1:1226502/150‑1 (MQ=255)
                                           gAAGTGCGGATCAGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGTTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                          <  2:1459848/150‑1 (MQ=255)
                                            aaGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGt                                                                                                         <  2:1522535/150‑1 (MQ=255)
                                              gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGTCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                       >  2:1031628/1‑150 (MQ=255)
                                              gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                       >  1:538703/1‑150 (MQ=255)
                                               tGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCTCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCTTCACTTCACGTCCGGAGAAGCCCTGTTCt                                                                                                      <  2:1193763/150‑1 (MQ=255)
                                                gCGGAGCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTg                                                                                                     <  2:647060/150‑1 (MQ=255)
                                                  ggATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcg                                                                                                   >  2:255876/1‑150 (MQ=255)
                                                  ggATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcg                                                                                                   >  1:443060/1‑150 (MQ=255)
                                                    aTCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGcgcc                                                                                                 >  1:825938/1‑150 (MQ=255)
                                                      cGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGcgccgc                                                                                               >  1:1076323/1‑150 (MQ=255)
                                                       ggCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGGTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCa                                                                                              >  1:1759647/1‑150 (MQ=255)
                                                       ggCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCa                                                                                              >  1:1837755/1‑150 (MQ=255)
                                                        gCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTAAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCAt                                                                                             <  1:34718/150‑1 (MQ=255)
                                                         cAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATg                                                                                            <  1:881486/150‑1 (MQ=255)
                                                          aaGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATgg                                                                                           <  2:524378/150‑1 (MQ=255)
                                                          aaGGGGAATATCTACCGGATTGAGCATAACCGCATGACCCATTTCGCGCCAGTTGTGGGCTATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCTTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTTTTATGCGCCGCATgg                                                                                           <  1:759958/150‑1 (MQ=255)
                                                            gTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGGCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAt                                                                                         <  2:1586761/150‑1 (MQ=255)
                                                            gTGCAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTGGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCTAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAt                                                                                         <  2:49966/150‑1 (MQ=255)
                                                             tGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATc                                                                                        <  1:543249/150‑1 (MQ=255)
                                                              ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGGAGGAGCCCTGTTCTGCGCCGCATGGATcg                                                                                       >  1:165691/1‑149 (MQ=255)
                                                              ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                       >  2:1441448/1‑150 (MQ=255)
                                                              ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                       >  1:130485/1‑150 (MQ=255)
                                                                aaTATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAGCCCTGTTCTGCGCCGCATGGATCTTc                                                                                     >  1:934853/1‑150 (MQ=255)
                                                                   atCTACCGCATTGAGCATCACCGCATGACGCATTTCGCGCCAGTTGTTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                  <  1:1683805/150‑1 (MQ=255)
                                                                   atCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                  <  1:590161/150‑1 (MQ=255)
                                                                   atCTACCGCATTGAGCATCACCGCATGACCCAGTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGc                                                                                  <  2:920245/150‑1 (MQ=255)
                                                                    tCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCa                                                                                 <  1:1441448/150‑1 (MQ=255)
                                                                    tCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCa                                                                                 <  1:723199/150‑1 (MQ=255)
                                                                     cTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAt                                                                                >  1:1581417/1‑150 (MQ=255)
                                                                      tACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATa                                                                               >  2:1595647/1‑150 (MQ=255)
                                                                       aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                              >  1:1350352/1‑150 (MQ=255)
                                                                       aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                              <  2:1837755/150‑1 (MQ=255)
                                                                        ccGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAgg                                                                             <  1:1517873/150‑1 (MQ=255)
                                                                          gCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCTGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTa                                                                           <  1:888276/150‑1 (MQ=255)
                                                                            aTTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGCTCTTCGGCATAGGTAAg                                                                         >  1:919031/1‑150 (MQ=255)
                                                                             ttGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGCTCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGGCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGa                                                                        <  1:1126027/150‑1 (MQ=255)
                                                                              tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATATGTAAGaa                                                                       <  2:1758037/150‑1 (MQ=255)
                                                                              tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGaa                                                                       <  2:919442/150‑1 (MQ=255)
                                                                              tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGaa                                                                       <  2:1765304/150‑1 (MQ=255)
                                                                               gAGCATCCCCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGGTTGTCGCGACGGGCAAGCCCGTCGCCCGTCGAATTACCGCCATCACTTCACGTCCGGAGAAGCGCTGGTCTGCGCCGCATGGATCTTCGGCTTAggacagagc                                                                      >  2:331203/1‑143 (MQ=255)
                                                                               gAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGGCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAc                                                                      >  2:73206/1‑150 (MQ=255)
                                                                               gAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAc                                                                      >  2:835773/1‑150 (MQ=255)
                                                                                aGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAca                                                                     <  1:1590219/150‑1 (MQ=255)
                                                                                 gCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcat                                                                    >  1:1791928/1‑150 (MQ=255)
                                                                                  cATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatc                                                                   >  1:588541/1‑150 (MQ=255)
                                                                                  cATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatc                                                                   >  2:681803/1‑150 (MQ=255)
                                                                                   aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                  <  1:997995/150‑1 (MQ=255)
                                                                                    tCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatcat                                                                 <  2:1378634/150‑1 (MQ=255)
                                                                                    tCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatcat                                                                 <  2:842800/150‑1 (MQ=255)
                                                                                    tCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatcat                                                                 <  2:693679/150‑1 (MQ=255)
                                                                                      aCCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTACGCCATCACTTCACGTCCGGTGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTc                                                               >  1:910333/1‑150 (MQ=255)
                                                                                        cGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAg                                                             <  2:473122/150‑1 (MQ=255)
                                                                                             gACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCtt                                                        >  1:1862645/1‑150 (MQ=255)
                                                                                                 cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                    >  2:1007975/1‑150 (MQ=255)
                                                                                                 cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                    <  1:1451309/150‑1 (MQ=255)
                                                                                                  aTTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCAt                                                   <  2:1076323/150‑1 (MQ=255)
                                                                                                     tCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCTCATGGATCTTCGGCATAGGTAAGAACATCAATCAGCCCTTTGCATaaa                                                <  1:92153/150‑1 (MQ=255)
                                                                                                      cgcgCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAg                                               <  2:1602590/150‑1 (MQ=255)
                                                                                                       gcgcCAGTTGGGGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                              <  1:276296/150‑1 (MQ=255)
                                                                                                             gTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAGGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGc                                        >  1:1772116/1‑150 (MQ=255)
                                                                                                                ggtggCGATCATGGTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGTAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtc                                     <  2:133298/150‑1 (MQ=255)
                                                                                                                   ggCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGCTCTTCGGCAGAGGTAAGAACATCATTCGGCCCTTTGCATAAAGCAATGGCTtcatc                                  >  1:93174/1‑150 (MQ=255)
                                                                                                                     cGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCaa                                >  1:856453/1‑150 (MQ=255)
                                                                                                                       aTCAGGGTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGATCAGCATGGATCTTCGGCATAGGTAAGAACATCATTCATCCCTTTGCATAAAGAAATGGCTTCATCAagc                              <  1:107384/150‑1 (MQ=255)
                                                                                                                         cATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagcag                            <  2:1799544/150‑1 (MQ=255)
                                                                                                                           tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                          >  1:1036158/1‑150 (MQ=255)
                                                                                                                           tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                          >  2:982242/1‑150 (MQ=255)
                                                                                                                           tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCAAGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                          >  2:1474657/1‑150 (MQ=255)
                                                                                                                            gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGCTCTTCGGCATAGGGAAGAACCTCCTTCCGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                         >  1:178154/1‑150 (MQ=255)
                                                                                                                             ttAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTCCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCAt                        <  2:1112981/150‑1 (MQ=255)
                                                                                                                                gTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGGAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTAATCAAGCAGCCATGCa                     >  2:264056/1‑150 (MQ=255)
                                                                                                                                  ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                   >  1:1564161/3‑150 (MQ=255)
                                                                                                                                  cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCCCGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACAACATTCAGCCCTTTGCATAAAGAAATGGCGTCATCAAGCAGCCATGCAcc                   >  2:1401346/1‑150 (MQ=255)
                                                                                                                                     gACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGtt                >  2:1398343/1‑150 (MQ=255)
                                                                                                                                          gCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTTGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGAt           >  1:788057/1‑150 (MQ=255)
                                                                                                                                          gCAAGCCGGGCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGAt           <  2:482917/150‑1 (MQ=255)
                                                                                                                                           cAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCTAGCAGCCATGCACCGTTGGGATc          >  1:289219/1‑150 (MQ=255)
                                                                                                                                             aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCTTAGGTAAGAAGATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa        >  2:153652/1‑150 (MQ=255)
                                                                                                                                               ccGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATATAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACg      >  1:807522/1‑150 (MQ=255)
                                                                                                                                                 ggTCGCCCGTCGAAATTCCGCCATCACTTCACGGCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGTCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGt    <  1:169133/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAA  >  NC_000913/2919759‑2920055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑