Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,744 A→T 7.0% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7440AT 7.0% 380.3 / 14.2 138intergenic (‑109/+20)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (73/53);  new base T (6/4);  total (81/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

Only 100 of 140 total aligned reads displayed.

GATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAG  >  NC_000913/2980595‑2980893
                                                                                                                                                     |                                                                                                                                                     
gATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACa                                                                                                                                                       <  2:585316/150‑1 (MQ=255)
 aTTCCCAGCTCATTCCTCACAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGATTAAGGAACGAGAGAACCGCCTCAACGGCCAAGAGCCCAATATCTATTAACGAAAAAAGGGCCGGATGTACAg                                                                                                                                                      >  2:1145461/1‑150 (MQ=255)
    cccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                                                   >  1:1042510/1‑150 (MQ=255)
    cccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                                                   >  1:47722/1‑150 (MQ=255)
        gCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCACCAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGTGCCGGATGTACAGCACATcc                                                                                                                                               >  2:1128470/1‑150 (MQ=255)
          tCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCgg                                                                                                                                             <  1:1143210/150‑1 (MQ=255)
           cATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGTCCAAGTGCCCAATCTCAAATAACGAAAAAAGGGCCGGCTGTACAGCACATCCGGc                                                                                                                                            >  1:170724/1‑150 (MQ=255)
           cATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGc                                                                                                                                            <  1:163763/150‑1 (MQ=255)
            aTTCCTCCCAGCAAACCAATTGTTTTCATACCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTAATAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                                                                           <  1:1135308/150‑1 (MQ=255)
             ttCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCACCAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGccc                                                                                                                                          <  1:1003474/150‑1 (MQ=255)
             ttCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGccc                                                                                                                                          >  2:291267/1‑150 (MQ=255)
                  cccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATCTCTGTTACCGCAACAAGGCTCAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGACCAATCTCTATTCGCGAAAAAAGGGCCTGATGTACCGCACATCCGCCCCGTGaa                                                                                                                                     >  1:274234/1‑150 (MQ=255)
                  cccAGCAAACCAATTGATTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaa                                                                                                                                     <  1:960655/150‑1 (MQ=255)
                    cAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCTCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAACAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                   <  1:463849/150‑1 (MQ=255)
                    aaGCAAACCAATTGTTTTCATTCCACTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGCAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                   <  1:249390/149‑1 (MQ=255)
                        aaaCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGCTGTACAGCACATCCGGCCCGTGAAATCAGa                                                                                                                               <  1:738632/150‑1 (MQ=255)
                          aCCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCATCAAGGCTGAATAAGGAACGAGACAAACGCCTCACCGGCAAAGTGCCCAATCTCTAGTAACGAAAAAAGGGCCGGATGTACATCACATCCGGCCCGTGAAATCAGAcc                                                                                                                             >  2:745459/1‑149 (MQ=255)
                            cAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAgccgcc                                                                                                                           >  2:1134633/1‑150 (MQ=255)
                             aaTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCg                                                                                                                          <  2:424489/150‑1 (MQ=255)
                                tGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGata                                                                                                                       >  2:1005161/1‑150 (MQ=255)
                                   tttCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAACAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                                                                    >  1:573502/1‑150 (MQ=255)
                                     tCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtct                                                                                                                  >  1:639753/1‑150 (MQ=255)
                                       aTTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGACAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAATTCTCa                                                                                                                >  1:624136/1‑150 (MQ=255)
                                         tCCTCTCTCCTCATGTGTGTTACCGCAACAAGGCTGAATAAGCAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAc                                                                                                              <  2:1671181/150‑1 (MQ=255)
                                          ccTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACt                                                                                                             >  2:1614366/1‑150 (MQ=255)
                                          ccTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACt                                                                                                             >  2:351537/1‑150 (MQ=255)
                                          ccTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAAAAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACt                                                                                                             <  1:370581/150‑1 (MQ=255)
                                            tctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtc                                                                                                           >  2:1053793/1‑150 (MQ=255)
                                               ctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                                                                        <  2:1291410/150‑1 (MQ=255)
                                                  cTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCt                                                                                                     >  2:1392345/1‑150 (MQ=255)
                                                      tgtgtgTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                                                                 >  1:796012/1‑150 (MQ=255)
                                                      tgtgtgTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                                                                 <  2:1346841/150‑1 (MQ=255)
                                                        tgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATc                                                                                               >  1:1840795/1‑150 (MQ=255)
                                                             tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGttt                                                                                          >  1:1403580/1‑150 (MQ=255)
                                                              aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCAATTAACGAAAAACGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                                                                         <  2:1516351/150‑1 (MQ=255)
                                                                cGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCg                                                                                       >  1:1281681/1‑150 (MQ=255)
                                                                    acaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCg                                                                                   <  2:1392701/150‑1 (MQ=255)
                                                                     caaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGa                                                                                  <  1:1617933/150‑1 (MQ=255)
                                                                      aacggagAATAAGGACCGAGACAAACGCCTCAAAGCCCACGTGCCCAAACTATATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGCCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGat                                                                                 <  2:144019/144‑1 (MQ=255)
                                                                        ggatGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCCTCAGTTTGCGTTCGatat                                                                               <  1:1751861/147‑1 (MQ=255)
                                                                        ggCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGatat                                                                               >  2:1819047/1‑150 (MQ=255)
                                                                         gCTTAATAAGGAACGAGACAAACTCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATg                                                                              <  1:1305591/150‑1 (MQ=255)
                                                                           tGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATggt                                                                            >  2:1761846/1‑148 (MQ=255)
                                                                           tGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                                                                            <  1:13686/150‑1 (MQ=255)
                                                                             aaTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                                                                          <  2:1632532/150‑1 (MQ=255)
                                                                               tAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                                                                        >  2:725004/1‑150 (MQ=255)
                                                                                 aGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCg                                                                      >  2:675650/1‑150 (MQ=255)
                                                                                      cGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCAATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACa                                                                 <  1:291267/150‑1 (MQ=255)
                                                                                       gagaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGACCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATAAGTTTGCGTTCGATATGTTCCAGCGTTACAt                                                                >  1:1396001/1‑150 (MQ=255)
                                                                                        agaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGAGAAATCAGACCCCGATATTTCTCAACTTCTCGCCTCCCATCAGAATGCGTTCGATAAGATCCAGCGTGACAtt                                                               >  2:1721489/1‑150 (MQ=255)
                                                                                           cAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                            <  2:964784/150‑1 (MQ=255)
                                                                                           cAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGGTCGATATGTTCCAGCGTGACAttttt                                                            >  2:41018/1‑150 (MQ=255)
                                                                                            aaaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTg                                                           <  1:216863/150‑1 (MQ=255)
                                                                                             aaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTgg                                                          <  2:1680160/150‑1 (MQ=255)
                                                                                               cGCTTCAACGGCTATGTGCAGAATCTCTATTAAAGAAATAAGGGCCTGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCATCTTCTCGCCTGCAATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGtt                                                        <  2:201028/150‑1 (MQ=255)
                                                                                                gCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCATGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGttt                                                       >  2:1356508/1‑150 (MQ=255)
                                                                                                 ccTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc                                                      >  2:1718579/1‑150 (MQ=255)
                                                                                                  cTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc                                                     >  2:1872388/1‑150 (MQ=255)
                                                                                                    cAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                   <  2:47722/150‑1 (MQ=255)
                                                                                                     aaCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAACTCAGACGCCGATATTTCTCTACTTCTGGCCTGCCATCAGTTTTGGTTCGATATGTTCCCGCGTGACATTTATGGTTTCAGGa                                                  >  2:189479/1‑150 (MQ=255)
                                                                                                     aaCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCATCTTCTCGCCTGCCATCAGTTTGCGTGCGATATGTTCCAGCGTGACATTTTTGGTTACCGGa                                                  >  1:264792/1‑150 (MQ=255)
                                                                                                      aCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                 <  1:1451185/150‑1 (MQ=255)
                                                                                                      aCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                 <  2:537599/150‑1 (MQ=255)
                                                                                                        ggCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATg                                               <  2:1597514/150‑1 (MQ=255)
                                                                                                           cAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACCTCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGGTCGATATGTGCCAGCGTGAGATTTTTGGTGTCCGGAATgcgc                                            >  2:946893/1‑150 (MQ=255)
                                                                                                           cAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGAAGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                            <  2:1574756/150‑1 (MQ=255)
                                                                                                            aaGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGcc                                           <  1:495557/150‑1 (MQ=255)
                                                                                                                  ccAATCTCTCTTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg                                     <  2:973980/150‑1 (MQ=255)
                                                                                                                       ctctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCAGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGGCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATg                                >  1:81605/1‑150 (MQ=255)
                                                                                                                       ctctATTAACGAAAAAAGGGACGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGTTATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATg                                <  1:12927/150‑1 (MQ=255)
                                                                                                                          tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGccc                             >  1:378349/1‑150 (MQ=255)
                                                                                                                            ttAACGAAAAAAGGGCCGGATGTACCGCACATCCGGCCCGTGAAATCAGCCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcac                           >  1:543779/1‑150 (MQ=255)
                                                                                                                            ttAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcac                           <  2:1050819/150‑1 (MQ=255)
                                                                                                                             tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                          >  1:1693443/1‑150 (MQ=255)
                                                                                                                             tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                          >  1:171472/1‑150 (MQ=255)
                                                                                                                             tAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                          <  1:351537/150‑1 (MQ=255)
                                                                                                                              aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaa                         >  1:1556146/1‑150 (MQ=255)
                                                                                                                              aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaa                         >  2:1287830/1‑150 (MQ=255)
                                                                                                                              aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaa                         >  2:951382/1‑150 (MQ=255)
                                                                                                                              aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaa                         >  1:186706/1‑150 (MQ=255)
                                                                                                                               aCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaaa                        >  1:1353705/1‑150 (MQ=255)
                                                                                                                                cGAAAAAAGGGCCGGATGTACAGCTCATCCGGCCCGTGAAATCAGACGCCGATAGTTCTCCACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTACTGCCCACCAAc                       >  1:279307/1‑150 (MQ=255)
                                                                                                                                   aaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTCATGCCCACCAACGCa                    >  1:344998/1‑150 (MQ=255)
                                                                                                                                    aaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCaa                   <  2:729658/150‑1 (MQ=255)
                                                                                                                                    aaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCAATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGATCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCaa                   <  2:1668000/150‑1 (MQ=255)
                                                                                                                                     aaaGGGGCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTATCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAt                  <  1:1562408/150‑1 (MQ=255)
                                                                                                                                     aaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAATTTTGGGTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAt                  <  1:1815984/150‑1 (MQ=255)
                                                                                                                                      acgggCCGGATGTGATGTACATCCGGACCGTGATCTCAGCCGCCGATATTTCTCTACTGCTCGCCTGCCATCTGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGGTTCCGGTATGAGACAGAAAGTATTGCCCACAAACGCatag                 >  1:1046518/3‑147 (MQ=255)
                                                                                                                                      aaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATg                 >  2:1014079/1‑150 (MQ=255)
                                                                                                                                       cgggCCGGATGTGCTGTACATCCGGCCCATGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGt                >  1:1368130/2‑150 (MQ=255)
                                                                                                                                         ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa             >  2:1010992/1‑150 (MQ=255)
                                                                                                                                         ggCCGGATGTGCTGTACATACGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCACAAAGTACTGCGCACAACCGCAATGttg              >  2:298183/1‑149 (MQ=255)
                                                                                                                                         ggCCGGATGTGCTGTACATACGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGAGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCAACAAACGCAATGTTc              >  1:30711/1‑150 (MQ=255)
                                                                                                                                           ccGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAg            <  1:889359/150‑1 (MQ=255)
                                                                                                                                           ccGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAg            <  2:1042510/150‑1 (MQ=255)
                                                                                                                                            cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgc           <  1:1360382/150‑1 (MQ=255)
                                                                                                                                                 gTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTACAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTAAGCGAAgt      >  2:945951/1‑150 (MQ=255)
                                                                                                                                                  tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgtg     >  1:979642/1‑150 (MQ=255)
                                                                                                                                                  tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgtg     >  1:1350462/1‑150 (MQ=255)
                                                                                                                                                    cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTa   >  1:795846/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAG  >  NC_000913/2980595‑2980893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: