Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,103 C→T 5.7% intergenic (+27/‑433) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1030CT 5.7% 391.8 / 7.9 123intergenic (+27/‑433)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (61/54);  new base T (5/2);  total (67/56)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTT  >  NC_000913/2986954‑2987252
                                                                                                                                                     |                                                                                                                                                     
cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGc                                                                                                                                                       <  1:677451/150‑1 (MQ=255)
 ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                      >  2:1352516/1‑150 (MQ=255)
 ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                      >  2:1578234/1‑150 (MQ=255)
        catGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGAATGGTTCAAAATTGTAGCAAttt                                                                                                                                               <  1:1351296/150‑1 (MQ=255)
         atGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtttt                                                                                                                                              <  2:675662/150‑1 (MQ=255)
          tGCCTACGCTGTCAACGTCTCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTTTCCAGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAttttt                                                                                                                                             >  1:163818/1‑149 (MQ=255)
          tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTg                                                                                                                                             <  2:750600/150‑1 (MQ=255)
          tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCAAATATCAGTTGTGGATGGTTCAAAATTGCTACAAttttt                                                                                                                                             >  1:367598/1‑149 (MQ=255)
           gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGa                                                                                                                                            <  2:1619671/150‑1 (MQ=255)
           gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGa                                                                                                                                            >  2:1579781/1‑150 (MQ=255)
            ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGTCGTTCCATCTGAAATCTTATTACGCTGATCTTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTATTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                           <  1:1562924/150‑1 (MQ=255)
            ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGTGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                           <  2:279324/150‑1 (MQ=255)
             cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAc                                                                                                                                          <  2:1262720/150‑1 (MQ=255)
             cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAc                                                                                                                                          <  2:729507/150‑1 (MQ=255)
             cTACGCTGTCAAAGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGTATCAATTTTGAAc                                                                                                                                          <  2:728689/150‑1 (MQ=255)
                    ggcAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATTTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAATTTGCTACAATTTTGAAccattcca                                                                                                                                   <  2:214433/148‑1 (MQ=255)
                    gTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattcca                                                                                                                                   <  1:716962/150‑1 (MQ=255)
                     tCAACGTATCTCATCCCGGCGATTAAACCCTGGCGTTCAATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTTGATGGTTAAAAATTGATACAATTTTTAAccattccat                                                                                                                                  >  2:407099/1‑150 (MQ=255)
                            ttctcATCCCGGCGCTGAAACGCTGTCGTTCCATCGGATATCTGATTTCGCTGATCGTGGGTATCCTGTGCGTATCTGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCat                                                                                                                           <  1:1353539/149‑1 (MQ=255)
                            aTCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCat                                                                                                                           >  2:1741376/1‑150 (MQ=255)
                              ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                         <  1:1405643/150‑1 (MQ=255)
                                 aTCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                                                                                      <  1:1407648/150‑1 (MQ=255)
                                 aTCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                                                                                      >  1:218800/1‑150 (MQ=255)
                                  tCCCGGCGCTTAAACCCTTGCGTTCCATCGGAAATCTGATTACGCTGATCGTGTGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATAtt                                                                                                                     <  1:1718247/150‑1 (MQ=255)
                                  tCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATAtt                                                                                                                     <  2:1321132/150‑1 (MQ=255)
                                      ggCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAtt                                                                                                                 >  1:176485/1‑150 (MQ=255)
                                        cgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTg                                                                                                               <  1:975271/150‑1 (MQ=255)
                                          cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCAGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtt                                                                                                             <  2:412343/150‑1 (MQ=255)
                                               aCCCTGGCGTTCCTACGGAAATCTGATAACGATGATCGGGGGTATCCTGTGCGTATTTTTAATTATATTTAGTTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATTATATTCATTTGTtattat                                                                                                        <  2:1227171/150‑1 (MQ=255)
                                               aCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTtattat                                                                                                        >  1:65711/1‑150 (MQ=255)
                                                 ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                      <  1:1555343/150‑1 (MQ=255)
                                                 ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                      <  2:1161085/150‑1 (MQ=255)
                                                   tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                    <  2:410961/150‑1 (MQ=255)
                                                    ggCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCAc                                                                                                   >  1:866263/1‑150 (MQ=255)
                                                     gCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTACAcc                                                                                                  >  1:1416079/1‑150 (MQ=255)
                                                          ccATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATc                                                                                             >  2:1517270/1‑150 (MQ=255)
                                                           cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCa                                                                                            >  1:1026342/1‑150 (MQ=255)
                                                           cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCATATCa                                                                                            >  2:915633/1‑150 (MQ=255)
                                                           cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGATAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCa                                                                                            >  2:1435471/1‑150 (MQ=255)
                                                            aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                           >  2:1442282/1‑150 (MQ=255)
                                                            aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                           <  2:134823/150‑1 (MQ=255)
                                                            aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                           >  2:1339215/1‑150 (MQ=255)
                                                             tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTAATTTGTTATTATTCAACACTATCACt                                                                                          >  2:1140550/1‑150 (MQ=255)
                                                              cgtaaaTCTGATTACGCTGATCGTGGGTATCCTGTTCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACtt                                                                                         <  1:1063381/147‑1 (MQ=255)
                                                               ggAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACtta                                                                                        >  2:148994/1‑149 (MQ=255)
                                                                  aaTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctct                                                                                     >  2:556023/1‑150 (MQ=255)
                                                                   aTCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACACTATCACTtctctc                                                                                    >  1:1794040/1‑150 (MQ=255)
                                                                    tCTGATTACTCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCg                                                                                   <  2:346641/150‑1 (MQ=255)
                                                                       gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                                                                                >  2:1465504/1‑150 (MQ=255)
                                                                       gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                                                                                >  1:276318/1‑150 (MQ=255)
                                                                            cGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTTTTATTATTCCACCCTATCACTTCTCTCGTCATATcc                                                                           >  1:406170/1‑150 (MQ=255)
                                                                            cGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATcc                                                                           >  1:650387/1‑150 (MQ=255)
                                                                              cTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                         >  1:885228/1‑150 (MQ=255)
                                                                                gATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGt                                                                       >  2:1743379/1‑150 (MQ=255)
                                                                                 aTCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGta                                                                      <  1:403572/150‑1 (MQ=255)
                                                                                 aTCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGta                                                                      <  1:205659/150‑1 (MQ=255)
                                                                                 aTCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATATGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGta                                                                      <  1:149604/150‑1 (MQ=255)
                                                                                   cGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtatt                                                                    <  2:1275560/150‑1 (MQ=255)
                                                                                     tGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtattat                                                                  >  2:1613341/1‑150 (MQ=255)
                                                                                      gggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtattata                                                                 >  2:1400447/1‑149 (MQ=255)
                                                                                       ggTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACACTATCACTTCTCTCGTCATATACTAGTATTATCa                                                                >  2:981436/1‑150 (MQ=255)
                                                                                       ggTATCCTGTGCGAATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTAAAATTTTGAACCATTCCATTTTTAATAATATTCATTTTTCATTAATACAAACTATAACTACTCTCGTAATATCCTATTATTAACa                                                                >  1:640454/1‑150 (MQ=255)
                                                                                        gTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCag                                                               <  1:386372/150‑1 (MQ=255)
                                                                                         tATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagt                                                              <  1:1797619/150‑1 (MQ=255)
                                                                                            ccTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttat                                                           >  1:1219480/1‑149 (MQ=255)
                                                                                             cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagt                                                          >  2:1680487/1‑150 (MQ=255)
                                                                                               gtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAgttagtta                                                        <  2:1295497/150‑1 (MQ=255)
                                                                                                  cGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAac                                                     <  1:992915/150‑1 (MQ=255)
                                                                                                  cGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAac                                                     <  2:754186/150‑1 (MQ=255)
                                                                                                   gTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAaca                                                    >  1:1186399/1‑150 (MQ=255)
                                                                                                    tATCGGTAATGTTCTTTATCTAAAATCAGTTGTGGATGGTTCAAAAATGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacac                                                   <  2:103595/150‑1 (MQ=255)
                                                                                                      tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                 <  2:1679560/150‑1 (MQ=255)
                                                                                                       cGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                                >  2:975216/1‑150 (MQ=255)
                                                                                                       cGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                                >  2:117820/1‑150 (MQ=255)
                                                                                                            aTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAagag                                           >  1:666959/1‑150 (MQ=255)
                                                                                                             tGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAgaga                                          <  1:832472/150‑1 (MQ=255)
                                                                                                              cttCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGAt                                         >  1:1209436/2‑150 (MQ=255)
                                                                                                                  tttAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTAATCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGa                                     <  1:1787244/150‑1 (MQ=255)
                                                                                                                     aGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACa                                  <  1:569362/150‑1 (MQ=255)
                                                                                                                     aGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACa                                  >  1:1462184/1‑150 (MQ=255)
                                                                                                                      gCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTACACCCTATCACTTATAAAGTCAAATCCAAGTATTATAAGTTTGTTAAACACACAAAGAAATATTAACaa                                 >  2:499485/1‑149 (MQ=255)
                                                                                                                         aaTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCtt                              <  2:1252086/150‑1 (MQ=255)
                                                                                                                         aaTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCtt                              <  1:1252317/150‑1 (MQ=255)
                                                                                                                         aaTATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCtt                              >  2:1016724/1‑150 (MQ=255)
                                                                                                                            atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                           <  2:1727695/150‑1 (MQ=255)
                                                                                                                             tCAGTTGTTGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                          <  1:1188226/150‑1 (MQ=255)
                                                                                                                                 ttGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGa                      >  1:1117938/1‑150 (MQ=255)
                                                                                                                                    tgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTa                   <  1:1352516/150‑1 (MQ=255)
                                                                                                                                     ggATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAt                  >  2:1717668/1‑150 (MQ=255)
                                                                                                                                     ggATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAATTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTAAAATATCACTAt                  >  2:928000/1‑150 (MQ=255)
                                                                                                                                       aTGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACTAAGAGATCTGAACATCTTAAATAAGACTAttt                <  1:477042/150‑1 (MQ=255)
                                                                                                                                       aTGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTACACCCGATCACTTCTCTCGTCATATCCTAGTATTTTAAGTTATTTAAACACAATAATAGATCTGAACATCGTAAATAAGAGAAttt                >  1:1782384/1‑150 (MQ=255)
                                                                                                                                         ggTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTaa              >  1:22391/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg             >  2:460239/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg             >  1:1407202/1‑150 (MQ=255)
                                                                                                                                          gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg             >  1:1681348/1‑150 (MQ=255)
                                                                                                                                           ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGa            >  1:88208/1‑150 (MQ=255)
                                                                                                                                             cAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATg          >  1:1012822/1‑150 (MQ=255)
                                                                                                                                                aaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCATATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttc       <  2:273230/150‑4 (MQ=255)
                                                                                                                                                    gCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACt   <  2:654345/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTT  >  NC_000913/2986954‑2987252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: