Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,129 G→A 5.1% intergenic (+28/+29) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1290GA 5.1% 314.7 / 3.9 99intergenic (+28/+29)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (51/41);  new base A (3/2);  total (55/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 104 total aligned reads displayed.

ACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGG  >  NC_000913/3044981‑3045272
                                                                                                                                                    |                                                                                                                                               
acccTACGGCTTTATCTATGTTAATAAACATGTCGACGGTAATGGCGATATGTCGCGATCACGTAAGAAGAGCTTTAAATTCTACACAGAGGTGTATGCCAGCAACGGCGAGAAGCGTTAAGTCGATGAAGTACCGGATGCAATACttgt                                                                                                                                                <  1:282528/147‑1 (MQ=255)
aCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgt                                                                                                                                                <  1:1399768/150‑1 (MQ=255)
 cGCTACGGCTTTATCTATGTGAATAAACAGGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgtt                                                                                                                                               <  1:1547005/150‑1 (MQ=255)
 cGCTACGGCTTTATCTACGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgtt                                                                                                                                               <  1:1361697/150‑1 (MQ=255)
  gCTACTGCTTTATCTATGTTAATAAACATGACGACGGTACTTGCGATTTGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                              <  2:1500497/150‑1 (MQ=255)
    tACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCa                                                                                                                                            <  2:28111/150‑1 (MQ=255)
       ggCTTTATCTATTTTAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATcc                                                                                                                                         <  2:297464/150‑1 (MQ=255)
          tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGt                                                                                                                                      <  2:1513719/150‑1 (MQ=255)
          tttATCTATGTGAATAAAAATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                      <  1:1344318/150‑1 (MQ=255)
            tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                    <  1:1267895/150‑1 (MQ=255)
            tATCAATGGGAATAAACATGTCGACGGTTCTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTTGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATTCAATACTTGTCGCATCCGGTCa                                                                                                                                    <  2:1683681/150‑1 (MQ=255)
             agcTATGTGACTAAACATGACGACGGGACTGGCGATATGTCGCGTTCACGTAACAAGAGCATAAACTCGTACAAAGAGGTGATTGCCCGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                   <  2:1382051/148‑1 (MQ=255)
             aTCTATGTGAATAAACATGACGACGGTAGTGGCGATTTGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTATGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAg                                                                                                                                   >  2:1130758/1‑150 (MQ=255)
               cTATGTGAATAATCATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGAc                                                                                                                                 <  2:665222/150‑1 (MQ=255)
                  tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAc                                                                                                                              >  2:825809/1‑150 (MQ=255)
                      aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTcc                                                                                                                          >  1:1836610/1‑150 (MQ=255)
                            cATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCGCTGttt                                                                                                                    >  2:40649/1‑150 (MQ=255)
                            cATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGttt                                                                                                                    <  1:27590/150‑1 (MQ=255)
                            cATGACGAAGGTACTGGCTATATGTCGCGTTCACGTAAGTAGAGCTTTTACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGttt                                                                                                                    <  2:1610650/150‑1 (MQ=255)
                             aTGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGtttt                                                                                                                   <  2:1362069/150‑1 (MQ=255)
                              tGACGACGGTTCTGGCGATATGTCGCGTTCACGTAAGAAGATCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGTCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTa                                                                                                                  <  2:1424701/150‑1 (MQ=255)
                                 cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                               >  2:1615920/1‑150 (MQ=255)
                                 cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                               >  2:656284/1‑150 (MQ=255)
                                      gTACAGGCGATATGTCGCGATCACGAAAGAAGAGCTATAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCCGAAGCAAAACTTGATCCATCCGCTCAGACAACATCCCGGGTTTATATcccgc                                                                                                          >  2:378740/1‑150 (MQ=255)
                                         cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                       <  2:315777/150‑1 (MQ=255)
                                         cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCACCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                       <  2:543871/150‑1 (MQ=255)
                                          tGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCccgcccgc                                                                                                      >  2:183509/1‑150 (MQ=255)
                                              gATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGATGTGATTGCCAGCAACGGCGAGAAGCTATAAGTCGATGAAGTACCGGATGAAATACTTTTTGCATCCGCTCAGACAACATCCCTGATTTATTTACCGCCCGCCaaa                                                                                                  >  1:229665/1‑150 (MQ=255)
                                                 atGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGTTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCg                                                                                               <  1:1217990/150‑1 (MQ=255)
                                                   gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                             <  2:1272690/150‑1 (MQ=255)
                                                   gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                             >  2:5477/1‑150 (MQ=255)
                                                        gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGCTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                        <  2:1385674/150‑1 (MQ=255)
                                                         ttCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACt                                                                                       >  2:501521/1‑150 (MQ=255)
                                                           cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                     >  2:1562456/1‑150 (MQ=255)
                                                           cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCACCAAATCGATAAAACTTc                                                                                     >  2:524005/1‑150 (MQ=255)
                                                            aCGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTcc                                                                                    <  1:54250/150‑1 (MQ=255)
                                                                aagaagAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTg                                                                                >  1:1241457/1‑150 (MQ=255)
                                                                 agaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGa                                                                               <  2:835964/150‑1 (MQ=255)
                                                                  gaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGAc                                                                              <  1:1566190/150‑1 (MQ=255)
                                                                  gaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGAc                                                                              >  1:913314/1‑150 (MQ=255)
                                                                         tttAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAagag                                                                       <  1:290502/150‑1 (MQ=255)
                                                                          ttAACTTGTACACAGCGGTGATTGCCACCAACGGCGAGAAGCTGTAACTCGATGAACAACCGGATGCCCTACTTGCTGCATCCGGTCAGACAACTTCACTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAgg                                                                      <  2:293677/150‑1 (MQ=255)
                                                                             aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                   <  2:1250432/150‑1 (MQ=255)
                                                                              cTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCttt                                                                  >  1:60311/1‑150 (MQ=255)
                                                                              cTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCttt                                                                  >  2:1699742/1‑150 (MQ=255)
                                                                              cTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCttt                                                                  >  1:1847752/1‑150 (MQ=255)
                                                                              cTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCttt                                                                  >  1:247147/1‑150 (MQ=255)
                                                                                ggTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTAt                                                                <  1:1010804/150‑1 (MQ=255)
                                                                                ggTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTAt                                                                >  1:1614546/1‑150 (MQ=255)
                                                                                   aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                             <  1:1368259/150‑1 (MQ=255)
                                                                                    cAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                            >  2:1198094/1‑150 (MQ=255)
                                                                                    cAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                            >  2:1436515/1‑150 (MQ=255)
                                                                                     aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                           <  1:804089/150‑1 (MQ=255)
                                                                                       agagGTGATTGCCAGCAACTGCGAGAAGCTTTAAGTCGATGAAGTACCGTATTCAATACTTTTTGCTTCCGGTCAGACAACTTACCTGTTTTATTTCCCGCCCGACAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtag                                                         <  1:1014547/150‑1 (MQ=255)
                                                                                        aagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                        >  1:613297/2‑150 (MQ=255)
                                                                                            tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAGTTCTTGTTGCATCCCGTCAGACAAGTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTTTCACTtagtaggc                                                    >  2:333697/1‑148 (MQ=255)
                                                                                             gATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCa                                                   >  1:1442000/1‑150 (MQ=255)
                                                                                              aTTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAg                                                  <  2:744657/150‑1 (MQ=255)
                                                                                                tGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGAc                                                <  1:1618114/150‑1 (MQ=255)
                                                                                                tGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGAc                                                >  2:841206/1‑150 (MQ=255)
                                                                                                 gCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACa                                               >  1:705495/1‑150 (MQ=255)
                                                                                                  ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                              >  1:349230/1‑150 (MQ=255)
                                                                                                  ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                              >  2:587093/1‑150 (MQ=255)
                                                                                                  ccAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                              >  2:1545033/1‑150 (MQ=255)
                                                                                                       aaCGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGcc                                         <  2:60311/150‑1 (MQ=255)
                                                                                                         cGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTg                                       >  1:1500896/1‑150 (MQ=255)
                                                                                                                aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                <  1:785161/150‑1 (MQ=255)
                                                                                                                aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAATTCCCTGTTTTATGTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                <  1:81233/150‑1 (MQ=255)
                                                                                                                  gCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCCCTTAGTAGCCAGACACTGGCCTGCGCGACctc                              >  2:84272/1‑150 (MQ=255)
                                                                                                                  gCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACctc                              >  1:1253305/1‑150 (MQ=255)
                                                                                                                      tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttct                          >  1:1283750/1‑150 (MQ=255)
                                                                                                                      tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttct                          >  1:483216/1‑150 (MQ=255)
                                                                                                                      tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAGTTCCCGCCCGCCAAATCGATAAACCTTCCCGTGACGTAAGAGGCGTGATCACTTCGTAGCCAGACAATGGCCTGCGCGCCCtcttct                          >  1:1181987/1‑150 (MQ=255)
                                                                                                                        aGTCGATGAAGTACCGCATGCAATACTTGTTGCATCCGGTAAGACAACTTCCCTGTTTTATTTCACGCCCGCCAAATCGATAAAACTTCCCGTGACGAAAGAGGCGTGATCACTTAGTAGCCAGACAATGGCCAGCGCGACCTCTTCTGc                        >  2:244432/1‑150 (MQ=255)
                                                                                                                         gTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGcc                       >  1:1415484/1‑150 (MQ=255)
                                                                                                                         gTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGcc                       <  1:825809/150‑1 (MQ=255)
                                                                                                                          tCGATGAAGTACCGGATGCAATTCTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCTATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCAGCCt                      <  2:1574532/150‑1 (MQ=255)
                                                                                                                               gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACACCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccc                 >  2:546362/1‑149 (MQ=255)
                                                                                                                               gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcca                 <  1:986004/150‑1 (MQ=255)
                                                                                                                                aaGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccac                <  2:861633/150‑1 (MQ=255)
                                                                                                                                 aGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccacc               >  1:1201568/1‑150 (MQ=255)
                                                                                                                                  gTACCGGCTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGTCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccacca              <  2:1161856/150‑1 (MQ=255)
                                                                                                                                     ccGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc           <  2:599528/150‑1 (MQ=255)
                                                                                                                                      cGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt          <  2:1836610/150‑1 (MQ=255)
                                                                                                                                      cGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt          >  1:728948/1‑150 (MQ=255)
                                                                                                                                      cGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt          >  1:1543462/1‑150 (MQ=255)
                                                                                                                                      cGGATGCAACAAGTATTGAATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCt          >  1:1527804/1‑150 (MQ=255)
                                                                                                                                       ggATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTg         >  1:139188/1‑150 (MQ=255)
                                                                                                                                       ggATGCAATACTTGTTGCATCCGGTCAGAAAACTTCCCTGTTTTATTTCCCGCACGCAAAATCGATAAAACTTCCCGTGACGTAAGAGCCTTTATCAATTAGACGCCAGACAAAGGCCTGCGCGACATCTTCTGCCTGTAAACCACGCTg         >  1:1655450/1‑150 (MQ=255)
                                                                                                                                       ggATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTg         >  1:682733/1‑150 (MQ=255)
                                                                                                                                        gATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGc        >  1:517059/1‑150 (MQ=255)
                                                                                                                                        gATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTGTTATTTCCCGCCAGCCATATCGATAAAACTTCCCGTGACGTTAGCGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGc        <  1:886411/150‑1 (MQ=255)
                                                                                                                                            cAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgg    >  2:1585623/1‑150 (MQ=255)
                                                                                                                                            cAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgg    >  1:447370/1‑150 (MQ=255)
                                                                                                                                            cAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACATAGTAGCCAGACAATGGCCTGCGCGACCTCTGCTGCCTCTCCACCACGCGGCATgg    >  1:265675/1‑150 (MQ=255)
                                                                                                                                            cAATACTTGTAGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTACTGCCTGTCCACAACGCTGCATgg    >  1:1398125/1‑150 (MQ=255)
                                                                                                                                             aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg   <  1:183509/150‑1 (MQ=255)
                                                                                                                                             aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg   >  2:1842228/1‑150 (MQ=255)
                                                                                                                                             aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACAACGCTGCATggg   >  2:894652/1‑150 (MQ=255)
                                                                                                                                              aTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgggg  <  2:613297/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGG  >  NC_000913/3044981‑3045272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: