Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,125,395 A→T 7.2% L22Q (CTG→CAG)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,3950AT 7.2% 316.7 / 7.8 111L22Q (CTG→CAG) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base A (60/42);  new base T (2/7);  total (62/49)
Fisher's exact test for biased strand distribution p-value = 4.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAT  >  NC_000913/3125253‑3125543
                                                                                                                                              |                                                                                                                                                    
cTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCt                                                                                                                                               >  1:922148/1‑150 (MQ=255)
   tgtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTc                                                                                                                                            >  1:663998/1‑150 (MQ=255)
    gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGAGAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                           >  1:308835/1‑150 (MQ=255)
    gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGGTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                           >  2:1141091/1‑150 (MQ=255)
     ttttAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCgg                                                                                                                                          >  1:1334480/1‑150 (MQ=255)
     ttttAAGCGGGACTTCGTTGCCTTCCAGCACAGGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCATTTCATCGCCCAGAAGCTGATAGGGTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCgg                                                                                                                                          <  1:1152282/150‑1 (MQ=255)
      tttAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGc                                                                                                                                         >  1:1428323/1‑150 (MQ=255)
          aGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTcc                                                                                                                                     >  2:1150990/1‑150 (MQ=255)
              tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                 <  1:746567/150‑1 (MQ=255)
              tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGAGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                 >  1:1310613/1‑150 (MQ=255)
               gACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgc                                                                                                                                >  2:1808049/1‑150 (MQ=255)
                ccTTCGTTGCCTTCCCGCACCTGTTTAATCAGAAAGATGCGCCCGCGCGGCCCGTCCAGTCCATCGCCCAGAAGCTGATAGGTTGGGCAGGTGGCGGTACAAACTCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCAgcgcg                                                                                                                               <  2:746129/149‑1 (MQ=255)
                aCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcg                                                                                                                               <  1:1399099/150‑1 (MQ=255)
                 cTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgc                                                                                                                              <  1:75437/150‑1 (MQ=255)
                 cTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgc                                                                                                                              >  1:134135/1‑150 (MQ=255)
                    cggtGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGGCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctg                                                                                                                           <  2:200688/147‑4 (MQ=255)
                    cGTTTCCTTCCAGCACCTGTTTAATCAGATACATGCGCCCGCGCGGCCAGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGAAGGATGCTGTCGGCTTCCAgcgcgcgcc                                                                                                                           >  2:1514253/1‑149 (MQ=255)
                     gTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctgt                                                                                                                          <  1:38574/150‑5 (MQ=255)
                     gTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcgg                                                                                                                          >  1:1074594/1‑149 (MQ=255)
                      ttGCCTTCCAGCACCTGTTTAATCCGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctgtg                                                                                                                         <  1:943919/150‑6 (MQ=38)
                        gCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCTTCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGtt                                                                                                                       <  1:958517/150‑1 (MQ=255)
                         ccTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                                                                                      >  1:272280/1‑150 (MQ=255)
                          cTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCt                                                                                                                     >  1:873658/1‑150 (MQ=255)
                             ccAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                                                                                  >  2:1042104/1‑150 (MQ=255)
                             ccAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                                                                                  >  2:830001/1‑150 (MQ=255)
                               aGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                                                                                                >  2:549471/1‑150 (MQ=255)
                                   ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtct                                                                                                            <  2:1288213/150‑1 (MQ=255)
                                    cTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtctc                                                                                                           <  2:922148/150‑1 (MQ=255)
                                        ttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGAGAGGTTGGGCAGGTTGCGGTTCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGGTCTGCCGCCACTCTACa                                                                                                       >  2:651071/1‑150 (MQ=255)
                                         tAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGTGCAGGTTGCGGTAGAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAg                                                                                                      <  1:501282/150‑1 (MQ=255)
                                          aaTCAGATAGATGCGCCCGCGGGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGt                                                                                                     <  2:1794008/150‑1 (MQ=255)
                                           aTCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGtt                                                                                                    >  1:1295388/1‑150 (MQ=255)
                                             cAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTaa                                                                                                  >  2:1545723/1‑150 (MQ=255)
                                              agatagatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTGCTGCCGCATCTCGGCAGTGAAt                                                                                                 >  2:1720043/1‑150 (MQ=255)
                                                  agatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTggg                                                                                             <  2:1599138/150‑1 (MQ=255)
                                                   gatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGCACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGGTAATTGGGt                                                                                            >  1:1299052/1‑150 (MQ=255)
                                                    ctGCGCCCGCGCGGCCCGTCCTGTGCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACATAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCTCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                           <  1:1339927/149‑1 (MQ=255)
                                                    atGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                           <  2:1115069/150‑1 (MQ=255)
                                                     tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCTTTCTGCCGCATCTCTGCAGTTAATTGGGttt                                                                                          >  2:313424/1‑150 (MQ=255)
                                                     tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGttt                                                                                          >  1:1041326/1‑150 (MQ=255)
                                                     tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCGGCAGGTAATTGGGttt                                                                                          >  1:1746416/1‑150 (MQ=255)
                                                        gcCCTCGCCGCCCGTCACGATCAGCGCCCCGAAGCGCATCCGGTGGGCAGGGTGCGGAACAAAATCCGCAGTGCACACACGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGGCGCATCTCTTGAGTTAATTGGGTTTGCa                                                                                       <  2:289269/150‑1 (MQ=255)
                                                           cgcgggTCCCGTCCACTTCTTCGCCCACAAGCTGAAAGCTTTCGCAGGGTGCTGTACAAACTCGGCAGTGAACACAGGCGCGCAGGATGCGGTCGGCATCCAGCGCGCGCGCGTTCTGCCGCATCTCTTGAGTTAATTGGGTTTGCATAg                                                                                    <  2:1687845/150‑1 (MQ=255)
                                                             cgcgGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGcc                                                                                  <  2:657363/150‑1 (MQ=255)
                                                                   ccGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGctcc                                                                            <  1:681525/150‑1 (MQ=255)
                                                                    cGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTACAGTTAATTGGGTTTGCATAGCCTGctcct                                                                           >  1:884883/1‑150 (MQ=255)
                                                                       ccAGTTCATCGTCCAGACGCTGATAGCTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGGTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa                                                                        <  2:550270/150‑1 (MQ=255)
                                                                       ccAGTTCATCGCCCAGAAGCTGATAGGTGGGGCAGGGTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa                                                                        <  1:877918/150‑1 (MQ=255)
                                                                        cAGTTCATCGCCCAGAAGCTGATAGCTTCGGCAGGTTGCGGCACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGGCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGGTTGCATAGCCTGCTCCTCaaa                                                                       <  2:287426/150‑1 (MQ=255)
                                                                          gTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGCACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCACTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGt                                                                     >  1:607956/1‑150 (MQ=255)
                                                                             cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcg                                                                                                                                 >  2:414036/1‑87 (MQ=25)
                                                                             cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTcc                                                                  >  1:93252/1‑150 (MQ=255)
                                                                              aTCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTTATGGGGTTTGCATAGCCTGCGCCTCCAAGTTCcg                                                                 >  2:83103/1‑150 (MQ=255)
                                                                               tCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCcgc                                                                >  1:1804760/1‑150 (MQ=255)
                                                                                 gCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGt                                                              <  1:969577/150‑1 (MQ=255)
                                                                                    cAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACa                                                           <  1:1545723/150‑1 (MQ=255)
                                                                                          cTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGAc                                                     >  1:1258411/1‑150 (MQ=255)
                                                                                              tAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCggg                                                 <  1:1150990/150‑1 (MQ=255)
                                                                                               aGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCgggg                                                <  1:646228/150‑1 (MQ=255)
                                                                                                 gTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGtt                                              <  2:663998/150‑1 (MQ=255)
                                                                                                     ggCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAac                                          >  1:1860431/1‑150 (MQ=255)
                                                                                                      gCAGGTTGCGGTACAAAATACGCAGTGTCCACAGTCGCGCAGTATTCTGTCTGCGTCGAGCGCGCGCGCGTTATGCTTCATCTCTTGAGTTAATTGGGTTTTCATTGCCTGCTCCTCAAAGTTACGCGTACATGCGACCGGGGTTAAaca                                         <  1:1132506/150‑1 (MQ=255)
                                                                                                        aGGTTGCTGGACAAAATCCGCAGTGATCACAGGCGCGGATGATGCTGTCCTCTTCCAGCGCGCTCGATTTCTGTCGCATCTCTTCAGTTTATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGGTAAACAcg                                       <  2:530101/150‑1 (MQ=255)
                                                                                                         ggTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCAGAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACAcgc                                      >  1:314944/1‑150 (MQ=255)
                                                                                                           ttGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGGTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACAcgccg                                    >  1:1636265/1‑150 (MQ=255)
                                                                                                            tGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGAGACCGGGGTTAAACAcgccgc                                   >  2:1367313/1‑150 (MQ=255)
                                                                                                             gCGGTAGAAAATCCGCAGTGATCACAGGCGCGCAGGATTCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTGCAGTTAATTGGGTTTGAATAGCCTGCTCCTCTAAGTTCCGCGTACACGCGACCGGGGTTAAACACGCCGCa                                  <  1:258758/150‑1 (MQ=255)
                                                                                                             gCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCa                                  >  1:1272799/1‑150 (MQ=255)
                                                                                                              cGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGAATGGCTGCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGGTCCGCGTACATGCGACCGGGGTTAAACAcgccgcta                                 >  2:166672/1‑148 (MQ=255)
                                                                                                               ggTACAAAATCCGCAGTGACCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAg                                >  1:1656638/1‑150 (MQ=255)
                                                                                                               ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGGACATGCGACCGGGGTTAAACACGCCGCAAg                                >  2:1716972/1‑150 (MQ=255)
                                                                                                               ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAg                                >  2:1086998/1‑150 (MQ=255)
                                                                                                                 tACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAggg                              >  2:438896/1‑150 (MQ=255)
                                                                                                                  aCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCCTCTCTTCAGTAAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAgggg                             >  2:927271/1‑149 (MQ=255)
                                                                                                                    aaaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCg                           >  1:68086/1‑150 (MQ=255)
                                                                                                                     aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTGAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAAGCGACCGGGGTTAAACACGCCGCAAGGGTCgc                          >  1:190260/1‑149 (MQ=255)
                                                                                                                     aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGa                          >  2:1476135/1‑150 (MQ=255)
                                                                                                                      aaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCGTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACCCGCCGCAAGGGTCgcg                         >  1:220247/1‑148 (MQ=255)
                                                                                                                      aaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCCTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAg                         >  1:214432/1‑150 (MQ=255)
                                                                                                                       aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGGATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACCCGCCGCAAGGGTCGAgc                        >  1:1512184/1‑150 (MQ=255)
                                                                                                                       aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgc                        <  2:1523197/150‑1 (MQ=255)
                                                                                                                        tCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgct                       <  2:1428323/150‑1 (MQ=255)
                                                                                                                            cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                   <  1:1343104/150‑1 (MQ=255)
                                                                                                                            cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                   <  2:616878/150‑1 (MQ=255)
                                                                                                                              gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                 <  1:407109/150‑1 (MQ=255)
                                                                                                                              gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                 <  1:830001/150‑1 (MQ=255)
                                                                                                                              gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                 <  2:858765/150‑1 (MQ=255)
                                                                                                                                 aaCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa              >  1:1395729/1‑150 (MQ=255)
                                                                                                                                 aaCACAGGAGCGCAGTATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCTTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa              <  2:873248/150‑1 (MQ=255)
                                                                                                                                    acaGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgcg           >  2:1377203/1‑149 (MQ=255)
                                                                                                                                     caGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCCTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGGTTAGgctg          >  2:1502431/1‑150 (MQ=255)
                                                                                                                                      aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGTCCGGGGTTAAACACGCCGCAAGGGTCGTGCTGCTGTTTTAgctgg         >  1:720289/1‑149 (MQ=255)
                                                                                                                                      aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc         >  2:683035/1‑150 (MQ=255)
                                                                                                                                      aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc         >  2:689616/1‑150 (MQ=255)
                                                                                                                                         cgcgcTGGAAGCCGACAGCATCCTGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg      >  1:1870204/1‑150 (MQ=255)
                                                                                                                                         cgcgcAGGCGGCGGTCGGCTTCCAGCGCGCGCGCGTGCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg      <  2:308835/150‑1 (MQ=255)
                                                                                                                                         cgcgcAGGATGCTGTCGGGTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCATCGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg      <  1:354247/150‑1 (MQ=255)
                                                                                                                                         cgcgcAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg      <  1:1713401/150‑1 (MQ=255)
                                                                                                                                             cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt  <  1:1831445/150‑1 (MQ=255)
                                                                                                                                             cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt  >  2:1563876/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAT  >  NC_000913/3125253‑3125543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑