Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 304,083 G→T 5.0% P34T (CCC→ACC)  ykgJ ← UPF0153 cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913304,0830GT 5.0% 415.1 / 5.6 135P34T (CCC→ACC) ykgJUPF0153 cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (73/54);  new base T (2/6);  total (75/60)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTACATCG  >  NC_000913/303934‑304229
                                                                                                                                                     |                                                                                                                                                  
 tctcTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGACTGGCGGGa                                                                                                                                                   <  2:168169/150‑1 (MQ=255)
   tctGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCTTGGCGGGAAt                                                                                                                                                 >  1:133838/1‑150 (MQ=255)
   tctGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAAt                                                                                                                                                 <  1:1466095/150‑1 (MQ=255)
   tctGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGGTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAAt                                                                                                                                                 <  2:864458/150‑1 (MQ=255)
    ctGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATa                                                                                                                                                >  1:550762/1‑150 (MQ=255)
    ctGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATa                                                                                                                                                >  2:1554635/1‑150 (MQ=255)
      gCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGt                                                                                                                                              <  2:572495/150‑1 (MQ=255)
            ggACGATCGAGTTTAATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCAGCAAGGGCAATACATCGGGGGTTATTCTGATTGGTACCGCTCAGGCATCGGAGAAAAGGGGATATTTGTTCAGTGATCCTGGCGGGAATAGTACCGcc                                                                                                                                        >  2:360761/1‑150 (MQ=255)
              aCGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGTAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAg                                                                                                                                      >  1:428957/1‑150 (MQ=255)
              aCGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAg                                                                                                                                      >  1:1360263/1‑150 (MQ=255)
               cgatcgatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGTACTATTCCCGCCAgg                                                                                                                                     <  1:1724557/150‑2 (MQ=255)
               cgatcgatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGTACTATTCCCGCCAgg                                                                                                                                     <  2:717247/150‑2 (MQ=255)
               cgatccatTTTTATATCTCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGTACTATTCCCGCCAgg                                                                                                                                     <  1:1704052/150‑2 (MQ=255)
                gatcgatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCa                                                                                                                                    >  1:763211/1‑150 (MQ=255)
                 ttcgatTTTTATATATCTTGCAGCTGGCTTTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGGTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAt                                                                                                                                   <  2:1144789/149‑1 (MQ=255)
                  tcgatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtc                                                                                                                                  <  2:597166/150‑1 (MQ=255)
                   cgatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcg                                                                                                                                 <  1:1692597/150‑1 (MQ=255)
                    gatTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgt                                                                                                                                >  1:976195/1‑150 (MQ=255)
                    gatTTTTATATATCGTGCAGCAGGCATTTTTGACCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgt                                                                                                                                >  2:1268696/1‑150 (MQ=255)
                      tttttATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgtcg                                                                                                                              >  1:508559/1‑150 (MQ=255)
                      tttttATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgtcg                                                                                                                              <  1:1554175/150‑1 (MQ=255)
                      tttttATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgtcg                                                                                                                              <  1:1664349/150‑1 (MQ=255)
                      tttttATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgtcg                                                                                                                              <  2:1851099/150‑1 (MQ=255)
                        tttATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTAACGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCCTCGTCGGc                                                                                                                            >  1:758788/1‑150 (MQ=255)
                             atatCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCgg                                                                                                                       >  2:675601/1‑150 (MQ=255)
                              tatCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGc                                                                                                                      >  1:1156270/1‑150 (MQ=255)
                               atCGTGGAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGcc                                                                                                                     >  2:1466374/1‑150 (MQ=255)
                                  gTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCATGGGCAATACATCGGGGGTTTTTCTGAATGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCCGCATCGTCGGCTTCGGCCCAg                                                                                                                  >  1:1384740/1‑150 (MQ=255)
                                   tGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGt                                                                                                                 <  2:696252/150‑1 (MQ=255)
                                      agcagGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaa                                                                                                              >  2:726882/1‑150 (MQ=255)
                                       gcagGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                             >  1:854107/1‑150 (MQ=255)
                                        ccggCATGTTGGCCCGGTGTGCGTGCAAGGGGATTACATCGTGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAATGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGGCGGCGTCGGACCAGTaaaaa                                                                                                            <  2:1199938/148‑1 (MQ=255)
                                         agGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAag                                                                                                           >  1:1294178/1‑150 (MQ=255)
                                         agGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAag                                                                                                           <  2:124700/150‑1 (MQ=255)
                                          ggCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaaata                                                                                                          >  1:895956/1‑148 (MQ=255)
                                            cATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATGTTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAgaga                                                                                                        >  2:630076/1‑150 (MQ=255)
                                            cATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAgaga                                                                                                        >  2:755939/1‑150 (MQ=255)
                                            cATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAgaga                                                                                                        >  1:1305878/1‑150 (MQ=255)
                                             aTTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGAc                                                                                                       <  2:745272/150‑1 (MQ=255)
                                              tttttGCCCGGGGTGCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGAcg                                                                                                      <  1:943074/150‑1 (MQ=255)
                                                   gCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGaa                                                                                                 >  2:141419/1‑150 (MQ=255)
                                                    cccGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGaaa                                                                                                >  2:525331/1‑150 (MQ=255)
                                                    cccGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGaaa                                                                                                >  1:1372002/1‑150 (MQ=255)
                                                      cGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGaaaaa                                                                                              >  2:1496569/1‑150 (MQ=255)
                                                       ggggTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAAt                                                                                             <  1:1413901/150‑1 (MQ=255)
                                                         ggTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGGGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGc                                                                                           <  2:1781096/150‑1 (MQ=255)
                                                          ggtCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGTTATTTGTTCAGTGAGGCTGGGGGGAATTGTATCGCCAGCATCGTCGGCTTCTGCCCAGTAAAAAGTGACGCGGAAAAATGca                                                                                          <  1:1126583/148‑1 (MQ=255)
                                                          gTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGca                                                                                          <  2:1586120/150‑1 (MQ=255)
                                                             ccTGCAAGGTCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGAATCGTTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGGAAAATGCacaa                                                                                       >  2:246678/1‑150 (MQ=255)
                                                               tGCAAGGGCAATACATCGGGGGTATTTCTGATTGGAACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGCGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGGGGAAAAATGCacaaca                                                                                     >  2:301492/1‑150 (MQ=255)
                                                                    gggCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcac                                                                                >  1:445264/1‑150 (MQ=255)
                                                                    gggCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTAGGACCAGTAAAAAGAGCCGCGGAAAAAAGCACAACAGGcac                                                                                >  2:335227/1‑150 (MQ=255)
                                                                     gtcAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGGTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcacc                                                                               <  1:367077/148‑1 (MQ=255)
                                                                     ggCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcacc                                                                               <  1:478745/150‑1 (MQ=255)
                                                                        aaTACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACcaca                                                                            >  1:380121/1‑150 (MQ=255)
                                                                            cATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTc                                                                        <  2:454619/150‑1 (MQ=255)
                                                                                gggggTTTTTCTGATTGGTACCGCTCATGCATCGTTGAAAAGTGGATATTTGTTCAGTGAGCCTGGCGGTAATAGTACCGCCAGCATCGGCGGCTTCGGCCCAGTAAATAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgc                                                                    <  1:245261/150‑1 (MQ=255)
                                                                                gggggTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgc                                                                    >  2:1036101/1‑150 (MQ=255)
                                                                                  gggTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgcat                                                                  <  2:1540326/150‑1 (MQ=255)
                                                                                      ttttCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGAt                                                              <  2:1817593/150‑1 (MQ=255)
                                                                                      ttttCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGAt                                                              >  1:1058342/1‑150 (MQ=255)
                                                                                            gATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACCCGTCATGCATGGATTCAGAt                                                        >  2:1205898/1‑150 (MQ=255)
                                                                                               tGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGc                                                     <  1:415446/150‑1 (MQ=255)
                                                                                                ggTACCGCTCCTGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCATGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACCACAGGCCCCACCCGTCATGCATGGATTCAGATTGCt                                                    >  2:1400190/1‑150 (MQ=255)
                                                                                                ggTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAAAGCACAACAGGCAACACACGTCATGCATGGATTCAGATTGCt                                                    >  1:512674/1‑150 (MQ=255)
                                                                                                  tcgcGCTCATGCATCGTTGAAAAGGGGATATTTGGTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCCGCTTCGGCCCAGTTAAAAGAGCCGCGGAAAAATGCACAACAGGCACCACACGGCATGCATGGATTCAGATTGCTCa                                                  <  2:256333/147‑1 (MQ=255)
                                                                                                   aCCGCTCATGCATCGGTGTAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCAt                                                 <  2:428957/150‑1 (MQ=255)
                                                                                                     cGCTCATGCATCGGTGAAAAGGTGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATaa                                               <  2:724668/150‑1 (MQ=255)
                                                                                                     cGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATaa                                               <  2:1046245/150‑1 (MQ=255)
                                                                                                      gCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACCCGCCCCACACGTCATGCATGGAGTCAGATTGCTCATAAt                                              >  2:943504/1‑150 (MQ=255)
                                                                                                      gCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAAt                                              >  2:526579/1‑150 (MQ=255)
                                                                                                      gCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAAt                                              <  2:1360263/150‑1 (MQ=255)
                                                                                                         cATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGTCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCa                                           <  1:1594594/150‑1 (MQ=255)
                                                                                                           tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCAcc                                         <  1:364763/150‑1 (MQ=255)
                                                                                                           tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCAcc                                         <  1:1466374/150‑1 (MQ=255)
                                                                                                             cATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAg                                       >  1:1643737/1‑150 (MQ=255)
                                                                                                                cGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTAc                                    <  2:1157581/150‑1 (MQ=255)
                                                                                                                    gAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGACCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGGCATGCTTGGATTCAGATTGCTCATAATTCACCAGTACAGCt                                >  2:971361/1‑150 (MQ=255)
                                                                                                                      aaaGGGGATATTTGTTCAGTGAGCCTGGTGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTaa                              >  1:1064387/1‑150 (MQ=255)
                                                                                                                          gggATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACCGGCACCACACGTCATGAATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAAt                          >  2:1695813/1‑150 (MQ=255)
                                                                                                                          gggATATTTGATCAGTGAGCCTGGCGTGAATAGTACCGCCATCATCGTCGGCATCTGCCCAGTAAAAAGAGACTCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAAt                          <  1:631860/150‑1 (MQ=255)
                                                                                                                           ggATATTTGTTCAGTGAGCCTGGCGGGATTAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACTCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCTTAATTGACCAGTACAGCGATAAATc                         >  1:283450/1‑150 (MQ=255)
                                                                                                                           ggATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGCGACGCGGAAACATGCACAACCGGCACCCCACGTCCTGCATCGAATCAGATTGCTCATAATTCACCAGTACAGCTAAAAATc                         >  1:98367/1‑150 (MQ=255)
                                                                                                                            gATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCg                        <  1:581585/150‑1 (MQ=255)
                                                                                                                             atatTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGt                       >  2:815781/1‑150 (MQ=255)
                                                                                                                               atTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTaa                     <  1:1055269/150‑1 (MQ=255)
                                                                                                                               atTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTaa                     <  2:1156270/150‑1 (MQ=255)
                                                                                                                               atTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTaa                     <  2:783523/150‑1 (MQ=255)
                                                                                                                                   gTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTTAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCCTGCATGGAGTCAGATTGCTCTGAATTCGCCAGTACAGCGAATCATCGTAAagag                 >  2:1176563/1‑150 (MQ=255)
                                                                                                                                   gTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAagag                 >  2:427247/1‑150 (MQ=255)
                                                                                                                                   gTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAagag                 <  1:804203/150‑1 (MQ=255)
                                                                                                                                        gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAg            >  2:1628321/1‑150 (MQ=255)
                                                                                                                                         tGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGc           <  2:525471/150‑1 (MQ=255)
                                                                                                                                           atgcTGGCGGTACTATTCCCGCCAGCATCGTCGGCTTCGGGCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCCCACGTCATGCATGGATTCAGATTGCTCATAATTCCCCAGTCCAGCTAAAAATCGTAAAGAGAACAGCgg         >  1:530716/4‑150 (MQ=255)
                                                                                                                                           aGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCTGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGATAACAGCgg         <  1:762477/150‑1 (MQ=255)
                                                                                                                                             ccTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGTACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTa       <  2:1395521/150‑1 (MQ=255)
                                                                                                                                              cTGGCGGTACTATTCCCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCCCCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTAc      >  2:743664/1‑150 (MQ=255)
                                                                                                                                                 gCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTACATc   <  1:115768/150‑1 (MQ=255)
                                                                                                                                                 gCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTACATc   >  1:663318/1‑150 (MQ=255)
                                                                                                                                                  cGGTAATAGTACCGCCAGCATCTTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACAAGTCATGCATGGATTCAGATTGCTCATATTTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTACATCg  <  1:139784/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAACAGCGGTACATCG  >  NC_000913/303934‑304229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑