Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,125,404 T→A 7.7% D19V (GAC→GTC)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,4040TA 7.7% 292.6 / 12.7 114D19V (GAC→GTC) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base T (61/41);  new base A (2/7);  total (63/51)
Fisher's exact test for biased strand distribution p-value = 3.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-02

Only 100 of 115 total aligned reads displayed.

TGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAA  >  NC_000913/3125256‑3125553
                                                                                                                                                    |                                                                                                                                                     
tgtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTc                                                                                                                                                      >  1:663998/1‑150 (MQ=255)
 gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGTTGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                     <  1:131598/150‑1 (MQ=255)
 gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGAGAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                     >  1:308835/1‑150 (MQ=255)
 gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGGTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                     >  2:1141091/1‑150 (MQ=255)
  ttttAAGCGTGCCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGGCCAGTTCATCGCCCAGAAGCTGATAGGTGGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGGCgg                                                                                                                                                    <  1:192989/150‑1 (MQ=255)
  ttttAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCgg                                                                                                                                                    >  1:1334480/1‑150 (MQ=255)
  ttttAAGCGGGACTTCGTTGCCTTCCAGCACAGGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCATTTCATCGCCCAGAAGCTGATAGGGTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCgg                                                                                                                                                    <  1:1152282/150‑1 (MQ=255)
   tttAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGc                                                                                                                                                   >  1:1428323/1‑150 (MQ=255)
           tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                           <  1:746567/150‑1 (MQ=255)
           tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGAGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                           >  1:1310613/1‑150 (MQ=255)
            gACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgc                                                                                                                                          >  2:1808049/1‑150 (MQ=255)
             aCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcg                                                                                                                                         <  1:1399099/150‑1 (MQ=255)
              cTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgc                                                                                                                                        <  1:75437/150‑1 (MQ=255)
                 cggtGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGGCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctg                                                                                                                                     <  2:200688/147‑4 (MQ=255)
                 cGTTTCCTTCCAGCACCTGTTTAATCAGATACATGCGCCCGCGCGGCCAGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGAAGGATGCTGTCGGCTTCCAgcgcgcgcc                                                                                                                                     >  2:1514253/1‑149 (MQ=255)
                  gTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctgt                                                                                                                                    <  1:38574/150‑5 (MQ=255)
                  gTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcgg                                                                                                                                    >  1:1074594/1‑149 (MQ=255)
                   ttGCCTTCCAGCACCTGTTTAATCCGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctgtg                                                                                                                                   <  1:943919/150‑6 (MQ=38)
                      ccTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                                                                                                >  1:272280/1‑150 (MQ=255)
                       cTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCt                                                                                                                               >  1:873658/1‑150 (MQ=255)
                          ccAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                                                                                            >  2:1042104/1‑150 (MQ=255)
                          ccAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                                                                                            >  2:830001/1‑150 (MQ=255)
                            aGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                                                                                                          >  2:549471/1‑150 (MQ=255)
                                ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtct                                                                                                                      <  2:1288213/150‑1 (MQ=255)
                                 cTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtctc                                                                                                                     <  2:922148/150‑1 (MQ=255)
                                     ttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGAGAGGTTGGGCAGGTTGCGGTTCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGGTCTGCCGCCACTCTACa                                                                                                                 >  2:651071/1‑150 (MQ=255)
                                      tAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGTGCAGGTTGCGGTAGAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAg                                                                                                                <  1:501282/150‑1 (MQ=255)
                                       aaTCAGATAGATGCGCCCGCGGGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGt                                                                                                               <  2:1794008/150‑1 (MQ=255)
                                        aTCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGtt                                                                                                              >  1:1295388/1‑150 (MQ=255)
                                          cAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTaa                                                                                                            >  2:1545723/1‑150 (MQ=255)
                                           agatagatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTGCTGCCGCATCTCGGCAGTGAAt                                                                                                           >  2:1720043/1‑150 (MQ=255)
                                               agatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTggg                                                                                                       <  2:1599138/150‑1 (MQ=255)
                                                gatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGCACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGGTAATTGGGt                                                                                                      >  1:1299052/1‑150 (MQ=255)
                                                 ctGCGCCCGCGCGGCCCGTCCTGTGCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACATAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCTCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                                     <  1:1339927/149‑1 (MQ=255)
                                                 atGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                                     <  2:1115069/150‑1 (MQ=255)
                                                  tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCTTTCTGCCGCATCTCTGCAGTTAATTGGGttt                                                                                                    >  2:313424/1‑150 (MQ=255)
                                                  tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGttt                                                                                                    >  1:1041326/1‑150 (MQ=255)
                                                  tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCGGCAGGTAATTGGGttt                                                                                                    >  1:1746416/1‑150 (MQ=255)
                                                        cgcgggTCCCGTCCACTTCTTCGCCCACAAGCTGAAAGCTTTCGCAGGGTGCTGTACAAACTCGGCAGTGAACACAGGCGCGCAGGATGCGGTCGGCATCCAGCGCGCGCGCGTTCTGCCGCATCTCTTGAGTTAATTGGGTTTGCATAg                                                                                              <  2:1687845/150‑1 (MQ=255)
                                                          cgcgGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGcc                                                                                            <  2:657363/150‑1 (MQ=255)
                                                                ccGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGctcc                                                                                      <  1:681525/150‑1 (MQ=255)
                                                                 cGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTACAGTTAATTGGGTTTGCATAGCCTGctcct                                                                                     >  1:884883/1‑150 (MQ=255)
                                                                 cGTCCAGATCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTGTGCATAGCCTGctcct                                                                                     >  2:355537/1‑150 (MQ=255)
                                                                    ccAGTTCATCGCCCAGAAGCTGATAGGTGGGGCAGGGTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa                                                                                  <  1:877918/150‑1 (MQ=255)
                                                                     cAGTTCATCGCCCAGAAGCTGATAGCTTCGGCAGGTTGCGGCACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGGCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGGTTGCATAGCCTGCTCCTCaaa                                                                                 <  2:287426/150‑1 (MQ=255)
                                                                          cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcg                                                                                                                                           <  1:414036/87‑1 (MQ=25)
                                                                          cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcg                                                                                                                                           >  2:414036/1‑87 (MQ=25)
                                                                          cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTcc                                                                            >  2:668802/1‑150 (MQ=255)
                                                                          cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTcc                                                                            >  1:93252/1‑150 (MQ=255)
                                                                           aTCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTTATGGGGTTTGCATAGCCTGCGCCTCCAAGTTCcg                                                                           >  2:83103/1‑150 (MQ=255)
                                                                            tCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCcgc                                                                          >  1:1804760/1‑150 (MQ=255)
                                                                              gCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGt                                                                        <  1:969577/150‑1 (MQ=255)
                                                                                 cAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACa                                                                     <  1:1545723/150‑1 (MQ=255)
                                                                                           tAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCggg                                                           <  1:1150990/150‑1 (MQ=255)
                                                                                            aGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCgggg                                                          <  1:646228/150‑1 (MQ=255)
                                                                                              gTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGtt                                                        <  2:663998/150‑1 (MQ=255)
                                                                                                  ggCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAac                                                    >  1:1860431/1‑150 (MQ=255)
                                                                                                   gCAGGTTGCGGTACAAAATACGCAGTGTCCACAGTCGCGCAGTATTCTGTCTGCGTCGAGCGCGCGCGCGTTATGCTTCATCTCTTGAGTTAATTGGGTTTTCATTGCCTGCTCCTCAAAGTTACGCGTACATGCGACCGGGGTTAAaca                                                   <  1:1132506/150‑1 (MQ=255)
                                                                                                     aGGTTGCTGGACAAAATCCGCAGTGATCACAGGCGCGGATGATGCTGTCCTCTTCCAGCGCGCTCGATTTCTGTCGCATCTCTTCAGTTTATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGGTAAACAcg                                                 <  2:530101/150‑1 (MQ=255)
                                                                                                        ttGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGGTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACAcgccg                                              >  1:1636265/1‑150 (MQ=255)
                                                                                                         tGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGAGACCGGGGTTAAACAcgccgc                                             >  2:1367313/1‑150 (MQ=255)
                                                                                                          gCGGTAGAAAATCCGCAGTGATCACAGGCGCGCAGGATTCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTGCAGTTAATTGGGTTTGAATAGCCTGCTCCTCTAAGTTCCGCGTACACGCGACCGGGGTTAAACACGCCGCa                                            <  1:258758/150‑1 (MQ=255)
                                                                                                          gCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCa                                            >  1:1272799/1‑150 (MQ=255)
                                                                                                           cGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGAATGGCTGCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGGTCCGCGTACATGCGACCGGGGTTAAACAcgccgcta                                           >  2:166672/1‑148 (MQ=255)
                                                                                                            ggTACAAAATCCGCAGTGACCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAg                                          >  1:1656638/1‑150 (MQ=255)
                                                                                                            ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGGACATGCGACCGGGGTTAAACACGCCGCAAg                                          >  2:1716972/1‑150 (MQ=255)
                                                                                                            ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAg                                          >  2:1086998/1‑150 (MQ=255)
                                                                                                              tACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAggg                                        >  2:438896/1‑150 (MQ=255)
                                                                                                               aCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCCTCTCTTCAGTAAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAgggg                                       >  2:927271/1‑149 (MQ=255)
                                                                                                                cAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTc                                      >  2:1032462/1‑150 (MQ=255)
                                                                                                                 aaaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCg                                     >  1:68086/1‑150 (MQ=255)
                                                                                                                  aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTGAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAAGCGACCGGGGTTAAACACGCCGCAAGGGTCgc                                    >  1:190260/1‑149 (MQ=255)
                                                                                                                  aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGa                                    >  2:1476135/1‑150 (MQ=255)
                                                                                                                   aaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCGTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACCCGCCGCAAGGGTCgcg                                   >  1:220247/1‑148 (MQ=255)
                                                                                                                   aaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCCTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAg                                   >  1:214432/1‑150 (MQ=255)
                                                                                                                    aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGGATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACCCGCCGCAAGGGTCGAgc                                  >  1:1512184/1‑150 (MQ=255)
                                                                                                                    aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgc                                  <  2:1523197/150‑1 (MQ=255)
                                                                                                                     tCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgct                                 <  2:1428323/150‑1 (MQ=255)
                                                                                                                         cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                             <  2:616878/150‑1 (MQ=255)
                                                                                                                         cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                             <  1:1343104/150‑1 (MQ=255)
                                                                                                                           gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                           <  2:858765/150‑1 (MQ=255)
                                                                                                                           gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                           <  1:407109/150‑1 (MQ=255)
                                                                                                                           gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                           <  1:830001/150‑1 (MQ=255)
                                                                                                                            tGAACACAGGCGCGCAGGATGCTGGCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGttt                          <  2:1438784/150‑1 (MQ=255)
                                                                                                                              aaCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa                        >  1:1395729/1‑150 (MQ=255)
                                                                                                                              aaCACAGGAGCGCAGTATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCTTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa                        <  2:873248/150‑1 (MQ=255)
                                                                                                                                 acaGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgcg                     >  2:1377203/1‑149 (MQ=255)
                                                                                                                                   aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGTCCGGGGTTAAACACGCCGCAAGGGTCGTGCTGCTGTTTTAgctgg                   >  1:720289/1‑149 (MQ=255)
                                                                                                                                   aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc                   >  2:683035/1‑150 (MQ=255)
                                                                                                                                   aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc                   >  2:689616/1‑150 (MQ=255)
                                                                                                                                      cgcgcAGGCGGCGGTCGGCTTCCAGCGCGCGCGCGTGCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                <  2:308835/150‑1 (MQ=255)
                                                                                                                                      cgcgcAGGATGCTGTCGGGTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCATCGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                <  1:354247/150‑1 (MQ=255)
                                                                                                                                      cgcgcAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                <  1:1713401/150‑1 (MQ=255)
                                                                                                                                          cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt            >  2:1563876/1‑150 (MQ=255)
                                                                                                                                          cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt            <  1:1831445/150‑1 (MQ=255)
                                                                                                                                               tGCTGTCGGCTTCCAGGGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCGCCTCAAAGGGCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGGCGAGCTGCTGTTAAAGCTGCTGGTGATAGCgg       <  1:1141091/150‑1 (MQ=255)
                                                                                                                                                 cTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATATCTTCAGTTAATTGGGTTTCAATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGaa     >  1:1083781/1‑150 (MQ=255)
                                                                                                                                                   gTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAGGGGTCGAGCTGGTGTTTAAGCTGCTGGTGAGAGCGGAATa   >  1:89372/1‑150 (MQ=255)
                                                                                                                                                   gTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATa   <  2:134135/150‑1 (MQ=255)
                                                                                                                                                    tCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATaa  <  1:549471/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAA  >  NC_000913/3125256‑3125553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: