Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,125,406 G→A 8.4% A18A (GCC→GCT)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,4060GA 8.4% 323.3 / 12.3 109A18A (GCC→GCT) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base G (57/41);  new base A (2/7);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 7.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 117 total aligned reads displayed.

GTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAG  >  NC_000913/3125257‑3125555
                                                                                                                                                     |                                                                                                                                                     
gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGTTGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                       <  1:131598/150‑1 (MQ=255)
gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGAGAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                       >  1:308835/1‑150 (MQ=255)
gtTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGGTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCg                                                                                                                                                       >  2:1141091/1‑150 (MQ=255)
 ttttAAGCGTGCCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGGCCAGTTCATCGCCCAGAAGCTGATAGGTGGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGGCgg                                                                                                                                                      <  1:192989/150‑1 (MQ=255)
  tttAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGc                                                                                                                                                     >  1:1428323/1‑150 (MQ=255)
          tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                             <  1:746567/150‑1 (MQ=255)
          tGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGAGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                                                                                             >  1:1310613/1‑150 (MQ=255)
           gACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgc                                                                                                                                            >  2:1808049/1‑150 (MQ=255)
            ccTTCGTTGCCTTCCCGCACCTGTTTAATCAGAAAGATGCGCCCGCGCGGCCCGTCCAGTCCATCGCCCAGAAGCTGATAGGTTGGGCAGGTGGCGGTACAAACTCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCAgcgcg                                                                                                                                           <  2:746129/149‑1 (MQ=255)
            aCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcg                                                                                                                                           <  1:1399099/150‑1 (MQ=255)
            aCTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcg                                                                                                                                           <  2:1693942/150‑1 (MQ=255)
             cTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgc                                                                                                                                          <  1:75437/150‑1 (MQ=255)
             cTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgc                                                                                                                                          >  1:134135/1‑150 (MQ=255)
                cggtGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGGCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctg                                                                                                                                       <  2:200688/147‑4 (MQ=255)
                cGTTTCCTTCCAGCACCTGTTTAATCAGATACATGCGCCCGCGCGGCCAGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGAAGGATGCTGTCGGCTTCCAgcgcgcgcc                                                                                                                                       >  2:1514253/1‑149 (MQ=255)
                 gTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcgg                                                                                                                                      >  1:1074594/1‑149 (MQ=255)
                  ttGCCTTCCAGCACCTGTTTAATCCGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcgcgcctgtg                                                                                                                                     <  1:943919/150‑6 (MQ=38)
                     ccTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                                                                                                  >  1:272280/1‑150 (MQ=255)
                         ccAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                                                                                              >  2:1042104/1‑150 (MQ=255)
                           aGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                                                                                                            >  2:549471/1‑150 (MQ=255)
                               ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtct                                                                                                                        <  2:1288213/150‑1 (MQ=255)
                                cTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAtctc                                                                                                                       <  2:922148/150‑1 (MQ=255)
                                     tAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGTGCAGGTTGCGGTAGAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAg                                                                                                                  <  1:501282/150‑1 (MQ=255)
                                       aTCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGtt                                                                                                                >  1:1295388/1‑150 (MQ=255)
                                         cAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTaa                                                                                                              >  2:1545723/1‑150 (MQ=255)
                                          agatagatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTGCTGCCGCATCTCGGCAGTGAAt                                                                                                             >  2:1720043/1‑150 (MQ=255)
                                               gatGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGCACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGGTAATTGGGt                                                                                                        >  1:1299052/1‑150 (MQ=255)
                                                ctGCGCCCGCGCGGCCCGTCCTGTGCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACATAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCTCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                                       <  1:1339927/149‑1 (MQ=255)
                                                atGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGtt                                                                                                       <  2:1115069/150‑1 (MQ=255)
                                                 tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCTTTCTGCCGCATCTCTGCAGTTAATTGGGttt                                                                                                      >  2:313424/1‑150 (MQ=255)
                                                 tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGttt                                                                                                      >  1:1041326/1‑150 (MQ=255)
                                                 tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCGGCAGGTAATTGGGttt                                                                                                      >  1:1746416/1‑150 (MQ=255)
                                                    gcCCTCGCCGCCCGTCACGATCAGCGCCCCGAAGCGCATCCGGTGGGCAGGGTGCGGAACAAAATCCGCAGTGCACACACGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGGCGCATCTCTTGAGTTAATTGGGTTTGCa                                                                                                   <  2:289269/150‑1 (MQ=255)
                                                       cgcgggTCCCGTCCACTTCTTCGCCCACAAGCTGAAAGCTTTCGCAGGGTGCTGTACAAACTCGGCAGTGAACACAGGCGCGCAGGATGCGGTCGGCATCCAGCGCGCGCGCGTTCTGCCGCATCTCTTGAGTTAATTGGGTTTGCATAg                                                                                                <  2:1687845/150‑1 (MQ=255)
                                                       cgcgcgGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAg                                                                                                >  1:1553982/1‑150 (MQ=255)
                                                         cgcgGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGcc                                                                                              <  2:657363/150‑1 (MQ=255)
                                                               ccGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGctcc                                                                                        <  1:681525/150‑1 (MQ=255)
                                                                cGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTACAGTTAATTGGGTTTGCATAGCCTGctcct                                                                                       >  1:884883/1‑150 (MQ=255)
                                                                   ccAGTTCATCGTCCAGACGCTGATAGCTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGGTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa                                                                                    <  2:550270/150‑1 (MQ=255)
                                                                   ccAGTTCATCGCCCAGAAGCTGATAGGTGGGGCAGGGTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCaa                                                                                    <  1:877918/150‑1 (MQ=255)
                                                                    cAGTTCATCGCCCAGAAGCTGATAGCTTCGGCAGGTTGCGGCACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGGCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGGTTGCATAGCCTGCTCCTCaaa                                                                                   <  2:287426/150‑1 (MQ=255)
                                                                      gTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGCACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCACTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGt                                                                                 >  1:607956/1‑150 (MQ=255)
                                                                        tCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTc                                                                               >  1:797692/1‑150 (MQ=255)
                                                                         cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcg                                                                                                                                             >  2:414036/1‑87 (MQ=25)
                                                                         cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCTGGAAGCCGACAGCATCCTgcg                                                                                                                                             <  1:414036/87‑1 (MQ=25)
                                                                         cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTcc                                                                              >  2:668802/1‑150 (MQ=255)
                                                                         cATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTcc                                                                              >  1:93252/1‑150 (MQ=255)
                                                                          aTCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTTATGGGGTTTGCATAGCCTGCGCCTCCAAGTTCcg                                                                             >  2:83103/1‑150 (MQ=255)
                                                                             gCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGt                                                                          <  1:969577/150‑1 (MQ=255)
                                                                                cAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACa                                                                       <  1:1545723/150‑1 (MQ=255)
                                                                                      cTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGAc                                                                 >  1:1258411/1‑150 (MQ=255)
                                                                                          tAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCggg                                                             <  1:1150990/150‑1 (MQ=255)
                                                                                           aGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCgggg                                                            <  1:646228/150‑1 (MQ=255)
                                                                                             gTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGtt                                                          <  2:663998/150‑1 (MQ=255)
                                                                                                 ggCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAac                                                      >  1:1860431/1‑150 (MQ=255)
                                                                                                  gCAGGTTGCGGTACAAAATACGCAGTGTCCACAGTCGCGCAGTATTCTGTCTGCGTCGAGCGCGCGCGCGTTATGCTTCATCTCTTGAGTTAATTGGGTTTTCATTGCCTGCTCCTCAAAGTTACGCGTACATGCGACCGGGGTTAAaca                                                     <  1:1132506/150‑1 (MQ=255)
                                                                                                    aGGTTGCTGGACAAAATCCGCAGTGATCACAGGCGCGGATGATGCTGTCCTCTTCCAGCGCGCTCGATTTCTGTCGCATCTCTTCAGTTTATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGGTAAACAcg                                                   <  2:530101/150‑1 (MQ=255)
                                                                                                     ggTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCAGAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACAcgc                                                  >  1:314944/1‑150 (MQ=255)
                                                                                                       ttGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGGTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACAcgccg                                                >  1:1636265/1‑150 (MQ=255)
                                                                                                        tGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGAGACCGGGGTTAAACAcgccgc                                               >  2:1367313/1‑150 (MQ=255)
                                                                                                         gCGGTAGAAAATCCGCAGTGATCACAGGCGCGCAGGATTCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTGCAGTTAATTGGGTTTGAATAGCCTGCTCCTCTAAGTTCCGCGTACACGCGACCGGGGTTAAACACGCCGCa                                              <  1:258758/150‑1 (MQ=255)
                                                                                                         gCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCa                                              >  1:1272799/1‑150 (MQ=255)
                                                                                                          cGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGAATGGCTGCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGGTCCGCGTACATGCGACCGGGGTTAAACAcgccgcta                                             >  2:166672/1‑148 (MQ=255)
                                                                                                           ggTACAAAATCCGCAGTGACCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAg                                            >  1:1656638/1‑150 (MQ=255)
                                                                                                           ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGGACATGCGACCGGGGTTAAACACGCCGCAAg                                            >  2:1716972/1‑150 (MQ=255)
                                                                                                           ggTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAg                                            >  2:1086998/1‑150 (MQ=255)
                                                                                                             tACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAggg                                          >  2:438896/1‑150 (MQ=255)
                                                                                                              aCAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCCTCTCTTCAGTAAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAgggg                                         >  2:927271/1‑149 (MQ=255)
                                                                                                               cAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTc                                        >  2:1032462/1‑150 (MQ=255)
                                                                                                                 aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCCGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTGAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACAAGCGACCGGGGTTAAACACGCCGCAAGGGTCgc                                      >  1:190260/1‑149 (MQ=255)
                                                                                                                 aaaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGa                                      >  2:1476135/1‑150 (MQ=255)
                                                                                                                  aaTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCCTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAg                                     >  1:214432/1‑150 (MQ=255)
                                                                                                                   aTCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgc                                    <  2:1523197/150‑1 (MQ=255)
                                                                                                                    tCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAgct                                   <  2:1428323/150‑1 (MQ=255)
                                                                                                                        cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                               <  2:616878/150‑1 (MQ=255)
                                                                                                                        cAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGctgctg                               <  1:1343104/150‑1 (MQ=255)
                                                                                                                          gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                             <  2:858765/150‑1 (MQ=255)
                                                                                                                          gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                             <  1:830001/150‑1 (MQ=255)
                                                                                                                          gTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGtt                             <  1:407109/150‑1 (MQ=255)
                                                                                                                             aaCACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa                          >  1:1395729/1‑150 (MQ=255)
                                                                                                                             aaCACAGGAGCGCAGTATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCTTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTaa                          <  2:873248/150‑1 (MQ=255)
                                                                                                                                acaGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgcg                       >  2:1377203/1‑149 (MQ=255)
                                                                                                                                 caGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCCTAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGGTTAGgctg                      >  2:1502431/1‑150 (MQ=255)
                                                                                                                                  aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGTCCGGGGTTAAACACGCCGCAAGGGTCGTGCTGCTGTTTTAgctgg                     >  1:720289/1‑149 (MQ=255)
                                                                                                                                  aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc                     >  2:683035/1‑150 (MQ=255)
                                                                                                                                  aGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAgctgc                     >  2:689616/1‑150 (MQ=255)
                                                                                                                                     cgcgcTGGAAGCCGACAGCATCCTGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                  >  1:1870204/1‑150 (MQ=255)
                                                                                                                                     cgcgcAGGCGGCGGTCGGCTTCCAGCGCGCGCGCGTGCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                  <  2:308835/150‑1 (MQ=255)
                                                                                                                                     cgcgcAGGATGCTGTCGGGTTCCAGCGCGCGCGCGTTCTGCCGCAGCTCTTCAGTTAATTGGGTTTGCATCGCCTGCTCCTCAAAGTTCCGCGTACATGCGCCCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                  <  1:354247/150‑1 (MQ=255)
                                                                                                                                     cgcgcAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTgg                  <  1:1713401/150‑1 (MQ=255)
                                                                                                                                         cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt              <  1:1831445/150‑1 (MQ=255)
                                                                                                                                         cAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAt              >  2:1563876/1‑150 (MQ=255)
                                                                                                                                              tGCTGTCGGCTTCCAGGGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCGCCTCAAAGGGCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGGCGAGCTGCTGTTAAAGCTGCTGGTGATAGCgg         <  1:1141091/150‑1 (MQ=255)
                                                                                                                                              ggcgggcGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTACACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCgg         <  2:1746416/144‑1 (MQ=255)
                                                                                                                                                cTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATATCTTCAGTTAATTGGGTTTCAATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGaa       >  1:1083781/1‑150 (MQ=255)
                                                                                                                                                  gTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAGGGGTCGAGCTGGTGTTTAAGCTGCTGGTGAGAGCGGAATa     >  1:89372/1‑150 (MQ=255)
                                                                                                                                                  gTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATa     <  2:134135/150‑1 (MQ=255)
                                                                                                                                                   tCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATaa    <  1:549471/150‑1 (MQ=255)
                                                                                                                                                     ggCTTCCATCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTACTTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGTGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAg  <  2:1310613/150‑1 (MQ=255)
                                                                                                                                                     ggCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGATAATTGGGTTTGAATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGATAAACACGCCGCAAGGCTAGATCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAg  >  1:288842/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAG  >  NC_000913/3125257‑3125555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑