Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,629 C→A 6.0% intergenic (‑258/+34) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6290CA 6.0% 438.9 / 7.7 152intergenic (‑258/+34)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (70/69);  new base A (7/5);  total (78/74)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

GTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCT  >  NC_000913/3136482‑3136778
                                                                                                                                                   |                                                                                                                                                     
gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                     <  1:1274367/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                    <  1:1607847/150‑1 (MQ=255)
   tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAt                                                                                                                                                  >  2:1712915/1‑150 (MQ=255)
    aTCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATa                                                                                                                                                 >  2:1247903/1‑150 (MQ=255)
     tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATaa                                                                                                                                                <  1:223539/150‑1 (MQ=255)
       aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATAATGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                              <  2:277438/150‑1 (MQ=255)
         aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                            >  2:295297/1‑150 (MQ=255)
         aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                            >  2:334601/1‑150 (MQ=255)
          cAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCAGTACTGATTATTCTTCATAATCAGt                                                                                                                                           >  1:361861/1‑150 (MQ=255)
           aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                          >  2:246686/1‑150 (MQ=255)
            aTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTATTAATAATAAGTaa                                                                                                                                         >  1:144296/1‑149 (MQ=255)
             tAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACg                                                                                                                                        >  2:207152/1‑150 (MQ=255)
              aaGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACgg                                                                                                                                       <  1:1131050/150‑1 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGTTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                      <  2:1076608/150‑1 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                      <  2:1557645/150‑1 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                      >  2:383203/1‑150 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggg                                                                                                                                      <  2:476953/150‑1 (MQ=255)
                tcattcattCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATACTCAGTACgggg                                                                                                                                     <  1:1201918/149‑1 (MQ=255)
                 caataattaaAAAACATTTGAAATAAACAATACCCAGGGATTCCAGTGGTTAATATTTTGCCGATTGAAATGAAAAAATATTTGAATATATAAAGTTTGAATATTTTGATTAATCACCGTACAGTTTATTCTTCATAAGCAGTACGGGGt                                                                                                                                    >  2:84808/6‑150 (MQ=255)
                      attcaAAACCTTTAGAAATAATCAAAAAACAGGTATTCCACTGGTTAATATATACACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAATCTTAATAATCAGTACTTGGTTGCTa                                                                                                                               >  1:360680/1‑150 (MQ=255)
                       ttcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTac                                                                                                                              <  2:1289742/150‑1 (MQ=255)
                         caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaa                                                                                                                            >  1:1108886/1‑150 (MQ=255)
                         caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGATacaa                                                                                                                            >  2:1012287/1‑150 (MQ=255)
                          aaaaCCTTTAGAAATAATAAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                           <  2:536783/150‑1 (MQ=255)
                           aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                          <  2:313739/150‑1 (MQ=255)
                              ccTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACAAATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGt                                                                                                                       <  1:1818289/150‑1 (MQ=255)
                               catgtgAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTa                                                                                                                      <  2:246429/145‑1 (MQ=255)
                               cTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTa                                                                                                                      <  1:1333743/150‑1 (MQ=255)
                                     aaaTAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACCGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACtt                                                                                                                <  2:858665/150‑1 (MQ=255)
                                       ataatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACtttt                                                                                                              <  2:1490135/150‑1 (MQ=255)
                                         aatCAAAACACATGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTc                                                                                                            <  2:1282204/150‑1 (MQ=255)
                                         aatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTc                                                                                                            <  2:840791/150‑1 (MQ=255)
                                         aatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTc                                                                                                            <  1:92236/150‑1 (MQ=255)
                                            cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                         <  2:1008569/150‑1 (MQ=255)
                                            cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                         >  1:1823845/1‑150 (MQ=255)
                                             aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                        <  1:1051366/150‑1 (MQ=255)
                                             aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                        >  2:609412/1‑150 (MQ=255)
                                                 cacaGGTATTCCACTGGTTAATATATTCACATATGAAATGAATATATATTGTAATATATAAATATTGAATATTTTGATTAATCCCCGAACTGATTATTATTCATAATCAGTACGGGGTTGCTAGAACATTTATTTCTTTATCACCACAAt                                                                                                    >  2:155742/1‑150 (MQ=255)
                                                      ttATTCCACTGGTTAATATAATCACATATGAAATTAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACg                                                                                               <  2:1170106/149‑1 (MQ=255)
                                                       tATTCCACTGGTTAATATATTCACATAAGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTATTCATAATCAGAACGGGGTTGCTACAACATGTATTAAATTTTCACCACAATTAActg                                                                                              >  2:144101/1‑148 (MQ=255)
                                                        aTTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGt                                                                                             <  1:1547592/150‑1 (MQ=255)
                                                        aTTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGt                                                                                             <  2:1199567/150‑1 (MQ=255)
                                                           ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACGACATGTATTACt                                                                                                                 <  2:1145302/127‑1 (MQ=255)
                                                           ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACGACATGTATTACt                                                                                                                 >  1:1145302/1‑127 (MQ=255)
                                                              atgtttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                       <  1:295297/146‑1 (MQ=255)
                                                                 gTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                    <  1:226477/150‑1 (MQ=255)
                                                                  ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                   <  2:1498584/150‑1 (MQ=255)
                                                                           tCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                          >  1:721684/1‑150 (MQ=255)
                                                                            cacaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACTGTTCAATATCACTCTCtttttt                                                                         >  2:685833/1‑150 (MQ=255)
                                                                             acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                        <  1:43250/150‑1 (MQ=255)
                                                                             acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                        <  2:826363/150‑1 (MQ=255)
                                                                             acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCCTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                        <  2:953586/150‑1 (MQ=255)
                                                                              caTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtga                                                                       <  1:1698439/150‑1 (MQ=255)
                                                                              caTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTAGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTAGCTACAACATGTATTACTATTTCACCACAATTAACGTTTCAATATCACTCTCTTTTTtga                                                                       >  2:641421/1‑150 (MQ=255)
                                                                              aatatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtga                                                                       <  1:1676748/149‑1 (MQ=255)
                                                                               atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                      <  1:1471205/150‑1 (MQ=255)
                                                                                tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                     <  1:81174/150‑1 (MQ=255)
                                                                                  tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCTCAACAATTAACGGTTCAATATCTCTCTCTTTTTTGATGAc                                                                   >  1:149941/1‑150 (MQ=255)
                                                                                  tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTAAATATCACTCTCTTTTTTGATGAc                                                                   >  2:807168/1‑150 (MQ=255)
                                                                                     aatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTTATCCCCGTACTGATTATTCATCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTATCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAg                                                                <  1:43962/150‑1 (MQ=255)
                                                                                     aatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAg                                                                >  2:383311/1‑150 (MQ=255)
                                                                                         aataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATAAGTACGGTGTTGCTACAACATGTATTACTTGTTCCCAAGAATTAAATGTTCAAGATCACGCTCATTTTTGATGACATGTGAt                                                            >  2:1132137/1‑150 (MQ=255)
                                                                                          ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                           >  1:208039/1‑150 (MQ=255)
                                                                                          aaaaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                           <  2:1862575/148‑1 (MQ=255)
                                                                                           taaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtc                                                          >  2:1799940/1‑150 (MQ=255)
                                                                                           taaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtc                                                          <  2:1108886/150‑1 (MQ=255)
                                                                                            aaataTTGTAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                         <  1:470654/150‑1 (MQ=255)
                                                                                               tatTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtcatca                                                      <  2:416648/150‑1 (MQ=255)
                                                                                                 ttGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCAcc                                                    >  2:171730/1‑150 (MQ=255)
                                                                                                  tGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcg                                                   >  1:902448/1‑150 (MQ=255)
                                                                                                   tgAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTAATCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                  <  1:343992/149‑1 (MQ=255)
                                                                                                   ggAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                  <  1:383032/150‑1 (MQ=255)
                                                                                                   ggAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                  >  1:743172/1‑150 (MQ=255)
                                                                                                         tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATTTCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                            >  1:687066/1‑150 (MQ=255)
                                                                                                         tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                            <  1:84610/150‑1 (MQ=255)
                                                                                                            aaaTATTGAATATTTTGATTAATCCCCGTTCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCACAAACACg                                         >  2:668869/1‑150 (MQ=255)
                                                                                                                   gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                  <  1:207152/150‑1 (MQ=255)
                                                                                                                   gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                  >  2:33491/1‑150 (MQ=255)
                                                                                                                   gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTGTTTTGATTACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                  >  1:6459/1‑150 (MQ=255)
                                                                                                                       atTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAg                              <  1:390012/150‑1 (MQ=255)
                                                                                                                       atTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAg                              <  1:1350304/150‑1 (MQ=255)
                                                                                                                        ttttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGt                             >  1:1706261/1‑150 (MQ=255)
                                                                                                                             aTTAATCCCCGTACTGATTTTTGTTCATAATCAGTACGGGGGTGCTACGACATGTATTCCTTTTTCACCACAATTAACGGTTCAATATCAGGCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                        >  1:1046158/1‑150 (MQ=255)
                                                                                                                             aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                        >  1:1157466/1‑150 (MQ=255)
                                                                                                                              ttAATCCCCGTACTGATTTTTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACAACAAATAACGGTTCAATATCACTCTCTATTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccgc                       >  1:881351/1‑150 (MQ=255)
                                                                                                                                 aTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                    >  1:322045/1‑150 (MQ=255)
                                                                                                                                 aTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                    >  1:855603/1‑150 (MQ=255)
                                                                                                                                  tCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAAc                   <  1:179221/150‑1 (MQ=255)
                                                                                                                                    cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                 <  2:1224360/150‑1 (MQ=255)
                                                                                                                                         aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg            >  1:726372/1‑150 (MQ=255)
                                                                                                                                          cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTATCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg           >  1:1223803/1‑150 (MQ=255)
                                                                                                                                          cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg           <  2:72136/150‑1 (MQ=255)
                                                                                                                                          cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg           >  2:1216611/1‑150 (MQ=255)
                                                                                                                                            gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa         >  2:455950/1‑150 (MQ=255)
                                                                                                                                            gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa         >  1:668732/1‑150 (MQ=255)
                                                                                                                                            gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa         >  1:650176/1‑150 (MQ=255)
                                                                                                                                              ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa       <  2:557966/150‑1 (MQ=255)
                                                                                                                                              ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTGCAATATCACTCTCTTTTTTGATGACCAGAGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa       <  1:96131/150‑1 (MQ=255)
                                                                                                                                                  tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCCCCGTAGTTGCCGCCAACGGTGAAGGTACATAcc   >  2:797757/1‑150 (MQ=255)
                                                                                                                                                   cttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt  >  1:185222/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCT  >  NC_000913/3136482‑3136778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: