Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,234,506 G→A 5.8% intergenic (‑51/+233) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,5060GA 5.8% 385.0 / 6.8 119intergenic (‑51/+233)higB/rlmGmRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (49/63);  new base A (5/2);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGC  >  NC_000913/3234358‑3234655
                                                                                                                                                    |                                                                                                                                                     
cccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa                                                                                                                                                      >  1:519296/1‑150 (MQ=255)
  cTTAGCAATCGTGTTCCCCAGAGCCACCATCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAtt                                                                                                                                                    <  1:615630/150‑1 (MQ=255)
  cTTAGCAATCGTGTTCCCCAGAGCCACCATCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAtt                                                                                                                                                    <  1:607943/150‑1 (MQ=255)
     cgCAAGCGTGTTCCCCAGCGCCCCCAACTCCGTTTTATGTTGCGGGTATTTTTCCGGAGCATCTTTCAATGCTGTTTGAGTAATCAGGTGCATTCTTCATCACGTCCGGGGACATATTGGCAATATGATAACATCCGTGGCCAGATTGGc                                                                                                                                                 <  2:130368/149‑1 (MQ=255)
     atcAATCGTGTTCCCCAGAGCCACCAACTCCGTTTAATGTTGCGGGTATTTATCCGCAGCATCTTTCAATGCTTTTTGAGTAATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                 <  1:1684157/148‑1 (MQ=255)
     aGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCAATCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                 <  1:1676764/150‑1 (MQ=255)
      gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCa                                                                                                                                                <  2:1831687/150‑1 (MQ=255)
          tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACgg                                                                                                                                            <  2:788686/150‑1 (MQ=255)
           ggtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTTCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCTCGTCCGTTGACTAATTGGCAATATGATAACATCCGTTTCCAGATTGGCAACgta                                                                                                                                           <  2:502071/149‑3 (MQ=255)
           cGTGTTCCGCTGAGCCACCTACTGCGTTTTATGTTGCGGGTATTTTTGCGCAGCATCTATCATTCCTTTTTGAGTTTTCATGTGCATTGTTCATCATGTCCGATGACAAATTGGCATTATGATAACATCCGTTGCCAATCTGGCAAGGGa                                                                                                                                           <  2:1839614/150‑1 (MQ=255)
           cGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGa                                                                                                                                           <  2:1487696/150‑1 (MQ=255)
            gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                          >  1:1331752/1‑150 (MQ=255)
            gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                          <  1:551359/150‑1 (MQ=255)
            gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                          >  2:37105/1‑150 (MQ=255)
               ttCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCTGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGaa                                                                                                                                       <  2:1063758/150‑1 (MQ=255)
                  cccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACATATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt                                                                                                                                    >  1:295822/1‑150 (MQ=255)
                   ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                   >  1:1070734/1‑150 (MQ=255)
                   ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTATTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                   >  2:521707/1‑150 (MQ=255)
                     agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATc                                                                                                                                 <  2:280925/150‑1 (MQ=255)
                      gagCCACAAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCt                                                                                                                                <  2:1012529/150‑1 (MQ=255)
                        gCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                              >  1:904890/1‑150 (MQ=255)
                         ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtg                                                                                                                             <  1:1291335/150‑1 (MQ=255)
                                cTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGga                                                                                                                      <  2:52260/150‑1 (MQ=255)
                                 tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaa                                                                                                                     >  1:1843609/1‑150 (MQ=255)
                                 tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaa                                                                                                                     <  2:1525589/150‑1 (MQ=255)
                                  ccGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATGACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                    <  1:595738/150‑1 (MQ=255)
                                  ccGTTTTATGTTGAGGGTATTTTTCCGCAGCATATTTCAATGCTTTATGAGTTAGCAGGTGCATTCTGCATCACGTCCGTGGACAAATTGGCAATATGATAACATCCGTTGCAAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                    <  1:154048/150‑1 (MQ=255)
                                    gTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCACTGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAAGgaagaa                                                                                                                  >  1:98848/1‑150 (MQ=255)
                                     ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGTTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt                                                                                                                 <  1:228132/150‑1 (MQ=255)
                                     ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt                                                                                                                 <  2:646875/150‑1 (MQ=255)
                                              gCGGGTATTTTTCCGCAGCATCTTTCAATGCGTTTTGAGTTAACAGGTGCATTCTACATCACGTCCGTTGAATAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATACACCTAGa                                                                                                        <  2:843525/150‑1 (MQ=255)
                                                 ggTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaa                                                                                                     >  2:1682039/1‑150 (MQ=255)
                                                   tATTTTTCCGCAGCATCTTTCAATGCTTTTTGATATATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTTGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc                                                                                                   <  1:1234603/150‑1 (MQ=255)
                                                     tttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGATGGAAGAATAAACGTAGAAAAACGc                                                                                                 <  1:1306613/150‑1 (MQ=255)
                                                      ttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCt                                                                                                >  1:536983/1‑150 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAACCGCTAAACAag                                                                                         >  1:1450088/1‑150 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                         <  2:1248739/150‑1 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                         <  2:616718/150‑1 (MQ=255)
                                                              agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga                                                                                        <  2:1275095/150‑1 (MQ=255)
                                                                caTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAgaga                                                                                      <  2:861667/150‑1 (MQ=255)
                                                                  tCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt                                                                                    >  1:327181/1‑150 (MQ=255)
                                                                    tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt                                                                                  >  1:1696777/1‑150 (MQ=255)
                                                                    tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAAATGGCAATATGATAACATCCTTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAACAATAAACGTAGAAAAACGCAAAACAATAGAATGt                                                                                  >  1:345174/1‑150 (MQ=255)
                                                                       cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                               >  2:806695/1‑150 (MQ=255)
                                                                          tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGa                                                                            >  1:190118/1‑150 (MQ=255)
                                                                          tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGATAAACAAGAGAATGTAGACGa                                                                            >  2:1723594/1‑150 (MQ=255)
                                                                            cTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATg                                                                          >  2:389656/1‑150 (MQ=255)
                                                                             ttttttAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                         <  2:181901/150‑1 (MQ=255)
                                                                             tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                         <  2:870114/150‑1 (MQ=255)
                                                                             tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                         >  2:140911/1‑150 (MQ=255)
                                                                             attttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                         <  1:240247/149‑1 (MQ=255)
                                                                              ttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGt                                                                        <  1:71503/150‑1 (MQ=255)
                                                                                ttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGa                                                                      >  1:1445607/1‑150 (MQ=255)
                                                                                    gTTATCAGGGGCATTCAGCATCACGTCCGTTGACAAATAGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAgg                                                                  <  2:904890/150‑1 (MQ=255)
                                                                                      tATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGa                                                                >  2:1229408/1‑150 (MQ=255)
                                                                                      tATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGa                                                                >  1:746784/1‑150 (MQ=255)
                                                                                       aTCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAt                                                               >  2:1322173/1‑150 (MQ=255)
                                                                                         cAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttt                                                             >  1:571794/1‑150 (MQ=255)
                                                                                         cAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttt                                                             <  1:1579033/150‑1 (MQ=255)
                                                                                           ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt                                                           >  2:1692961/1‑150 (MQ=255)
                                                                                           ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt                                                           <  2:1331752/150‑1 (MQ=255)
                                                                                            gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt                                                          <  2:602285/150‑1 (MQ=255)
                                                                                             tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTg                                                         <  1:702270/150‑1 (MQ=255)
                                                                                              gCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt                                                        >  1:123731/1‑150 (MQ=255)
                                                                                               cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt                                                       <  2:1524733/150‑1 (MQ=255)
                                                                                               cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt                                                       >  2:546579/1‑150 (MQ=255)
                                                                                                  tcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                    <  1:854698/150‑1 (MQ=255)
                                                                                                    ttcATCACGTCCGTTGACAAATTTGCAATATGATAACATCCGATGCCAGATTGTCAACGGATGAATTTATCTGTGTGACGGAAGAATCTACGTAGAAAAACTCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATg                                                  <  1:263757/150‑1 (MQ=255)
                                                                                                        tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTCGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAg                                              >  2:399052/1‑150 (MQ=255)
                                                                                                           cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACTCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcgc                                           <  2:1056990/150‑1 (MQ=255)
                                                                                                           cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATGTTTGGTTTTATGGAAgcgc                                           <  2:220039/150‑1 (MQ=255)
                                                                                                               cGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt                                       >  2:1267048/1‑150 (MQ=255)
                                                                                                                  tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTACACAAGAGAATGTAGAAGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                    <  2:46016/150‑1 (MQ=255)
                                                                                                                  tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                    <  2:1105260/150‑1 (MQ=255)
                                                                                                                  tGACAAATTGGCAATATGATAACATCCGTTGCCAGAGTGGCAACGGATGAATTTATATGTGTGACGTAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGAAAGTGATTTTTTTTTTTATGTAAGATCGATTCCa                                    >  1:1243874/1‑150 (MQ=255)
                                                                                                                   gACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAGCAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAg                                   >  2:260939/1‑150 (MQ=255)
                                                                                                                   gACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAg                                   >  1:999815/1‑150 (MQ=255)
                                                                                                                     cAAATTGGCAATATGATAACATCCGTTGCCAGAGTGGCTACGGATGAATTTATCTGTGTGACGGAAGAATAAACGAAGAAAAACGCTAAACAAGAGAATGTAAAAGATGGTGACAGAGATTTTTTGTTTAATGGAAGCGCGAATCCAGaa                                 >  2:112349/1‑150 (MQ=255)
                                                                                                                         ttGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg                             <  1:670923/150‑1 (MQ=255)
                                                                                                                          tGGCAATATGATAACTTCCGTTGCCAGCTTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTggg                            <  2:1631273/150‑1 (MQ=255)
                                                                                                                            gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                          <  2:327181/150‑1 (MQ=255)
                                                                                                                            gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                          <  2:388299/150‑1 (MQ=255)
                                                                                                                              aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGCGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                        <  1:491997/150‑1 (MQ=255)
                                                                                                                              aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                        <  2:1445607/150‑1 (MQ=255)
                                                                                                                                  tGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                    >  1:446632/1‑150 (MQ=255)
                                                                                                                                  tGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                    >  2:1866089/1‑150 (MQ=255)
                                                                                                                                    aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc                  <  2:295822/150‑1 (MQ=255)
                                                                                                                                    aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc                  >  2:1522418/1‑150 (MQ=255)
                                                                                                                                     tAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACTGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGTAGCGCGATTCCAGAACTGGGCAGACATTGcc                 <  2:562518/150‑1 (MQ=255)
                                                                                                                                       tcATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCgg               >  1:608190/2‑150 (MQ=255)
                                                                                                                                        cATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa              >  1:1737061/1‑150 (MQ=255)
                                                                                                                                         aTCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTTCCGGAt             >  1:1570725/1‑150 (MQ=255)
                                                                                                                                          tCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc           >  1:1064315/1‑150 (MQ=255)
                                                                                                                                          tCCGGTGCCAGATAGGCAACGGAAGAATTTATCTGTGTGACGGACGAATACCCGTAGAAAAACGCTAAACAAGAGAATGTAGACGATCGTGACTGGGATTTTTTGATTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATg            <  2:226650/150‑1 (MQ=255)
                                                                                                                                           ccGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGGGAGAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc           >  2:791905/1‑150 (MQ=255)
                                                                                                                                              ttGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGAc        <  1:1322173/150‑1 (MQ=255)
                                                                                                                                               tGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACg       <  2:275429/150‑1 (MQ=255)
                                                                                                                                                gCCAGATTGTCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGc      <  1:521707/150‑1 (MQ=255)
                                                                                                                                                    gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGc  >  1:392856/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGc  <  1:13743/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGC  >  NC_000913/3234358‑3234655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: