Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,258 G→A 6.3% intergenic (‑453/‑99) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,2580GA 6.3% 422.0 / 12.7 144intergenic (‑453/‑99)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (61/72);  new base A (3/6);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

Only 100 of 145 total aligned reads displayed.

TATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGT  >  NC_000913/314110‑314406
                                                                                                                                                    |                                                                                                                                                    
tATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCgg                                                                                                                                                     >  1:739838/1‑150 (MQ=255)
 aTCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGGTATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGa                                                                                                                                                    >  2:346137/1‑150 (MQ=255)
 aTCTATTTAATGACTTGCACAAAAAGCTAAATATTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGa                                                                                                                                                    <  1:654215/150‑1 (MQ=255)
    tATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                 >  1:1808212/1‑150 (MQ=255)
     aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAg                                                                                                                                                <  1:806423/150‑1 (MQ=255)
       ttAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGa                                                                                                                                              <  1:939076/150‑1 (MQ=255)
       ttAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGa                                                                                                                                              <  1:1781994/150‑1 (MQ=255)
        tAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCCCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGGGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                             <  1:832358/150‑1 (MQ=255)
         aaTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGaaa                                                                                                                                            <  1:1765692/150‑1 (MQ=255)
         aaTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaaa                                                                                                                                            <  2:1022214/150‑1 (MQ=255)
          aTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCTCTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAt                                                                                                                                           <  1:1375085/150‑1 (MQ=255)
          aTGACTTCCACAAAAAGCGAACTTATCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGCTGAGTTGCTATAGTCACTGGATATATTAGGTGGCGGTGCACTTACTTACATACAGGTATTTCCTTTTCCGCAGAAAAGGAAAt                                                                                                                                           <  1:84557/150‑1 (MQ=255)
           tGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATc                                                                                                                                          >  1:605652/1‑150 (MQ=255)
            gACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCg                                                                                                                                         >  1:1074640/1‑150 (MQ=255)
             aCTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                        <  1:1396054/150‑1 (MQ=255)
                 gCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtaccttta                                                                                                                                    <  1:1333903/150‑8 (MQ=255)
                       aaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTTGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                              >  2:779541/1‑150 (MQ=255)
                          gCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGtt                                                                                                                           <  2:518173/150‑1 (MQ=255)
                           cTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGATATCGGGAAATCACCGGttt                                                                                                                          >  2:1117343/1‑150 (MQ=255)
                              aaTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTc                                                                                                                       <  1:82097/150‑1 (MQ=255)
                               aTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCt                                                                                                                      <  2:245762/150‑1 (MQ=255)
                                ttttCCCCCATAAATAAAAATATAATCCCGCGCCCAACCCCCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTg                                                                                                                     <  2:1848702/150‑1 (MQ=255)
                                ttttCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTg                                                                                                                     <  1:1227412/150‑1 (MQ=255)
                                ttttCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTg                                                                                                                     <  2:495256/150‑1 (MQ=255)
                                      cccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                               <  2:1310698/150‑1 (MQ=255)
                                       ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCa                                                                                                              >  1:379653/1‑150 (MQ=255)
                                          taaataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAgcc                                                                                                           >  1:857561/1‑148 (MQ=255)
                                           aaataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCAGGTTTTTCTGACAAGCAGACg                                                                                                          >  2:1791212/1‑150 (MQ=255)
                                            aataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGc                                                                                                         >  2:1217508/1‑150 (MQ=255)
                                              taaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCa                                                                                                       >  1:417213/1‑150 (MQ=255)
                                                aaaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                     <  2:1635168/150‑1 (MQ=255)
                                                  aaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                                   <  1:209834/150‑1 (MQ=255)
                                                     ataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtg                                                                                                <  1:546584/150‑1 (MQ=255)
                                                     ataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAGCGGGAAATCCCCGGTTTTTCTGACAAGCAGCCGCCATTAgttt                                                                                                >  2:1364974/1‑149 (MQ=255)
                                                      taATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTGTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgt                                                                                               >  2:357879/1‑150 (MQ=255)
                                                       aaTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgtg                                                                                              <  1:1552385/150‑1 (MQ=255)
                                                         tCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTc                                                                                            <  2:894074/150‑1 (MQ=255)
                                                         tCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTc                                                                                            <  2:1824417/150‑1 (MQ=255)
                                                          cccGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCt                                                                                           >  2:312076/1‑150 (MQ=255)
                                                          cccGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCt                                                                                           >  1:1638372/1‑150 (MQ=255)
                                                            cgcgCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGc                                                                                         >  2:1455565/1‑150 (MQ=255)
                                                             gcgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGcc                                                                                        <  1:1810232/150‑1 (MQ=255)
                                                                cccAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTAt                                                                                     <  2:1512562/150‑1 (MQ=255)
                                                                 ccAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATg                                                                                    >  2:228856/1‑150 (MQ=255)
                                                                  cAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTTCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGt                                                                                   >  1:1394702/1‑150 (MQ=255)
                                                                  cAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGt                                                                                   >  1:833195/1‑150 (MQ=255)
                                                                        ccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTa                                                                             >  1:406797/1‑150 (MQ=255)
                                                                        ccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTa                                                                             <  1:1177292/150‑1 (MQ=255)
                                                                        ccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTa                                                                             >  2:873025/1‑150 (MQ=255)
                                                                                  ggCTATAGGCACTGGATATATTAGGTGGCGGTGCACGTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACCAGCAGACGCCATTAATTGTGTCTGCCTATGTTCGTTAATTCGTgcat                                                                   >  2:1330518/1‑150 (MQ=255)
                                                                                    cTATAGGCACTGGGTATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                                 <  1:346137/150‑1 (MQ=255)
                                                                                    cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                                 <  1:302795/150‑1 (MQ=255)
                                                                                    cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                                 <  1:1666196/150‑1 (MQ=255)
                                                                                     tataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAg                                                                <  1:893736/150‑1 (MQ=255)
                                                                                     tataGGCACTGGATATATTAGGTGGCGGTGAACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAACGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCTATATTTGTGTCTGCCTATGGTCGGTTATTAGTTCATCAg                                                                >  2:531458/1‑150 (MQ=255)
                                                                                         ggCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGaaa                                                            >  1:1847227/1‑150 (MQ=255)
                                                                                           cACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTTCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAAtt                                                          <  1:943206/150‑1 (MQ=255)
                                                                                              tGGATATATTAGGTGGCGGTTCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGATATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtc                                                       <  1:287952/150‑1 (MQ=255)
                                                                                               ggATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtct                                                      <  2:364782/150‑1 (MQ=255)
                                                                                                     atTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGt                                                <  2:1071665/150‑1 (MQ=255)
                                                                                                       tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                              <  1:1750579/150‑1 (MQ=255)
                                                                                                       tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                              >  2:1471100/1‑150 (MQ=255)
                                                                                                         ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACACGCAGACGCCATTATTTGTGTCTGCGTATGTTCGTGAATTCGTTCATCAGGAAATTATCTCATTGTcaca                                            >  2:80797/1‑150 (MQ=255)
                                                                                                         ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTcaca                                            <  2:597614/150‑1 (MQ=255)
                                                                                                           tGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACAtt                                          <  1:575105/150‑1 (MQ=255)
                                                                                                              cGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCTGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATtata                                       <  2:212450/150‑1 (MQ=255)
                                                                                                               ggTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                      >  2:623050/1‑150 (MQ=255)
                                                                                                                  gCACTATCTTACATTAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                   <  1:104008/150‑1 (MQ=255)
                                                                                                                   cACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACa                                  <  1:1561256/150‑1 (MQ=255)
                                                                                                                    aCTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGATATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAg                                 <  2:256245/150‑1 (MQ=255)
                                                                                                                       ttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTc                              <  2:465891/150‑1 (MQ=255)
                                                                                                                        tCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCa                             <  1:873025/150‑1 (MQ=255)
                                                                                                                           tACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATaa                          >  1:1289709/1‑150 (MQ=255)
                                                                                                                            aCATAAAGGTATTTCCTTTTCTGCTGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCATATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATaaa                         <  1:652314/150‑1 (MQ=255)
                                                                                                                             cATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGATTTCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaac                        >  2:330015/1‑150 (MQ=255)
                                                                                                                             cATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaac                        >  2:548897/1‑150 (MQ=255)
                                                                                                                              aTAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaaca                       <  1:513978/150‑1 (MQ=255)
                                                                                                                               tAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                      >  2:682886/1‑150 (MQ=255)
                                                                                                                                aaaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTATTTCTGACAAGCAGACGCCATTATATGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaac                     >  2:1627921/1‑150 (MQ=255)
                                                                                                                                    gTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACg                 <  2:739838/150‑1 (MQ=255)
                                                                                                                                    cgaTTTCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTGTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCagg                 >  1:878854/3‑148 (MQ=255)
                                                                                                                                     gaTTTCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGa                >  1:788832/2‑150 (MQ=255)
                                                                                                                                        ttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt             >  1:1043485/1‑150 (MQ=255)
                                                                                                                                        ttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt             >  2:1708320/1‑150 (MQ=255)
                                                                                                                                         tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCTGGAAATTATCTCAATGTCACATTATAAAACAGGTCATATCCAACCACGATTTc            >  1:1239417/1‑150 (MQ=255)
                                                                                                                                         tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtttg            >  2:1723533/1‑149 (MQ=255)
                                                                                                                                         tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTc            <  2:1847227/150‑1 (MQ=255)
                                                                                                                                         tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTc            >  1:1723557/1‑150 (MQ=255)
                                                                                                                                          ccTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCg           >  2:750849/1‑150 (MQ=255)
                                                                                                                                            ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGtt         <  1:1635496/150‑1 (MQ=255)
                                                                                                                                              ttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTAt       <  1:1012917/150‑1 (MQ=255)
                                                                                                                                                cTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTc     <  1:509110/150‑1 (MQ=255)
                                                                                                                                                cTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTc     >  1:790265/1‑150 (MQ=255)
                                                                                                                                                 tGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCa    >  1:990103/1‑150 (MQ=255)
                                                                                                                                                 tGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCa    <  2:1571385/150‑1 (MQ=255)
                                                                                                                                                  tcGGAATAGGATATCGTGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCTTCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAg   <  2:469063/149‑1 (MQ=255)
                                                                                                                                                  gCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAg   >  2:1219106/1‑150 (MQ=255)
                                                                                                                                                  gCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGGTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAACCAGGTCATAAACACCCACGAGTTCGGTAtgaag   >  1:351289/1‑146 (MQ=255)
                                                                                                                                                   cGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGt  >  1:485217/1‑150 (MQ=255)
                                                                                                                                                   cGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGt  <  2:1669164/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGT  >  NC_000913/314110‑314406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: