Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,234,507 A→T 6.3% intergenic (‑52/+232) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,5070AT 6.3% 349.0 / 6.5 119intergenic (‑52/+232)higB/rlmGmRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (48/62);  new base T (5/3);  total (53/66)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCC  >  NC_000913/3234358‑3234656
                                                                                                                                                     |                                                                                                                                                     
  cTTAGCAATCGTGTTCCCCAGAGCCACCATCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAtt                                                                                                                                                     <  1:615630/150‑1 (MQ=255)
  cTTAGCAATCGTGTTCCCCAGAGCCACCATCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAtt                                                                                                                                                     <  1:607943/150‑1 (MQ=255)
   ttAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTg                                                                                                                                                    <  1:491474/150‑1 (MQ=255)
     atcAATCGTGTTCCCCAGAGCCACCAACTCCGTTTAATGTTGCGGGTATTTATCCGCAGCATCTTTCAATGCTTTTTGAGTAATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                  <  1:1684157/148‑1 (MQ=255)
     aGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCAATCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                  <  1:1676764/150‑1 (MQ=255)
      gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCa                                                                                                                                                 <  2:1831687/150‑1 (MQ=255)
          tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACgg                                                                                                                                             <  2:788686/150‑1 (MQ=255)
           ggtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTTCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCTCGTCCGTTGACTAATTGGCAATATGATAACATCCGTTTCCAGATTGGCAACgta                                                                                                                                            <  2:502071/149‑3 (MQ=255)
           cGTGTTCCGCTGAGCCACCTACTGCGTTTTATGTTGCGGGTATTTTTGCGCAGCATCTATCATTCCTTTTTGAGTTTTCATGTGCATTGTTCATCATGTCCGATGACAAATTGGCATTATGATAACATCCGTTGCCAATCTGGCAAGGGa                                                                                                                                            <  2:1839614/150‑1 (MQ=255)
           cGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGa                                                                                                                                            <  2:1487696/150‑1 (MQ=255)
            gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                           <  1:551359/150‑1 (MQ=255)
            gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                           >  1:1331752/1‑150 (MQ=255)
             tgtTCCCCAGAGCCACCAACTCCGTTTTAAGTTGCGGGTATTTTACCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCAATCTTCATCCCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATCTGGCAACGGATg                                                                                                                                          <  1:55981/150‑1 (MQ=255)
               ttCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCTGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGaa                                                                                                                                        <  2:1063758/150‑1 (MQ=255)
                  cccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACATATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt                                                                                                                                     >  1:295822/1‑150 (MQ=255)
                   ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                    >  1:1070734/1‑150 (MQ=255)
                   ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTATTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                    >  2:521707/1‑150 (MQ=255)
                     agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCTCAGCATCTTTCATTTCTTTTTGAGTTATCATGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCTGTTTGGCAACGGATGAATTTATc                                                                                                                                  <  2:1683407/150‑1 (MQ=255)
                     agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATc                                                                                                                                  <  2:280925/150‑1 (MQ=255)
                       cgCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtg                                                                                                                                <  2:1331729/149‑1 (MQ=255)
                        gCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                               >  1:904890/1‑150 (MQ=255)
                         ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtg                                                                                                                              <  1:1291335/150‑1 (MQ=255)
                                cTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGga                                                                                                                       <  2:52260/150‑1 (MQ=255)
                                 tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaa                                                                                                                      >  1:1843609/1‑150 (MQ=255)
                                 tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaa                                                                                                                      <  2:1525589/150‑1 (MQ=255)
                                  ccGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATGACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                     <  1:595738/150‑1 (MQ=255)
                                  ccGTTTTATGTTGAGGGTATTTTTCCGCAGCATATTTCAATGCTTTATGAGTTAGCAGGTGCATTCTGCATCACGTCCGTGGACAAATTGGCAATATGATAACATCCGTTGCAAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                     <  1:154048/150‑1 (MQ=255)
                                    gTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCACTGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAAGgaagaa                                                                                                                   >  1:98848/1‑150 (MQ=255)
                                     ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt                                                                                                                  <  2:646875/150‑1 (MQ=255)
                                              gCGGGTATTTTTCCGCAGCATCTTTCAATGCGTTTTGAGTTAACAGGTGCATTCTACATCACGTCCGTTGAATAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATACACCTAGa                                                                                                         <  2:843525/150‑1 (MQ=255)
                                                 ggTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaa                                                                                                      >  2:1682039/1‑150 (MQ=255)
                                                  gTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaaa                                                                                                     >  2:603642/1‑150 (MQ=255)
                                                   tATTTTTCCGCAGCATCTTTCAATGCTTTTTGATATATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTTGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc                                                                                                    <  1:1234603/150‑1 (MQ=255)
                                                     tttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGATGGAAGAATAAACGTAGAAAAACGc                                                                                                  <  1:1306613/150‑1 (MQ=255)
                                                      ttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCt                                                                                                 >  1:536983/1‑150 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAACCGCTAAACAag                                                                                          >  1:1450088/1‑150 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                          <  2:1248739/150‑1 (MQ=255)
                                                             cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                          <  2:519296/150‑1 (MQ=255)
                                                              agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga                                                                                         <  2:1275095/150‑1 (MQ=255)
                                                              agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga                                                                                         <  2:381646/150‑1 (MQ=255)
                                                                caTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAgaga                                                                                       <  2:861667/150‑1 (MQ=255)
                                                                    tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAAATGGCAATATGATAACATCCTTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAACAATAAACGTAGAAAAACGCAAAACAATAGAATGt                                                                                   >  1:345174/1‑150 (MQ=255)
                                                                     ttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCCATGACAAATTGGCAATATGATAACATCCGTAGCAAGATTGGCAACGGATGAATTTATATGTGTGACGGAAGAAAAAACGTAGAAAAACTCTAAACAATAGAATGTa                                                                                  >  1:274241/1‑150 (MQ=255)
                                                                       cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                                >  2:806695/1‑150 (MQ=255)
                                                                       cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                                <  1:1872786/150‑1 (MQ=255)
                                                                          tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGa                                                                             >  1:190118/1‑150 (MQ=255)
                                                                          tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGATAAACAAGAGAATGTAGACGa                                                                             >  2:1723594/1‑150 (MQ=255)
                                                                            cTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATg                                                                           >  2:389656/1‑150 (MQ=255)
                                                                             ttttttAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  2:181901/150‑1 (MQ=255)
                                                                             tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  2:870114/150‑1 (MQ=255)
                                                                             attttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  1:240247/149‑1 (MQ=255)
                                                                              ttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGt                                                                         <  1:71503/150‑1 (MQ=255)
                                                                                ttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGa                                                                       >  1:1445607/1‑150 (MQ=255)
                                                                                   aGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAACCGCTAAACAAGAGAATGTAGACGATGGTGACAg                                                                    >  2:280006/1‑150 (MQ=255)
                                                                                    gTTATCAGGGGCATTCAGCATCACGTCCGTTGACAAATAGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAgg                                                                   <  2:904890/150‑1 (MQ=255)
                                                                                      tATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGa                                                                 >  1:746784/1‑150 (MQ=255)
                                                                                      tATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGa                                                                 >  2:1229408/1‑150 (MQ=255)
                                                                                       aTCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAt                                                                >  2:1322173/1‑150 (MQ=255)
                                                                                         cAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttt                                                              <  1:1579033/150‑1 (MQ=255)
                                                                                           ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt                                                            <  2:1331752/150‑1 (MQ=255)
                                                                                            gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt                                                           <  2:602285/150‑1 (MQ=255)
                                                                                             tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTg                                                          <  1:702270/150‑1 (MQ=255)
                                                                                              gCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt                                                         >  1:123731/1‑150 (MQ=255)
                                                                                               cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt                                                        >  2:546579/1‑150 (MQ=255)
                                                                                                  tcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                     >  2:1516988/1‑150 (MQ=255)
                                                                                                  tcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                     <  1:854698/150‑1 (MQ=255)
                                                                                                    ttcATCACGTCCGTTGACAAATTTGCAATATGATAACATCCGATGCCAGATTGTCAACGGATGAATTTATCTGTGTGACGGAAGAATCTACGTAGAAAAACTCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATg                                                   <  1:263757/150‑1 (MQ=255)
                                                                                                      catcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGa                                                 >  2:1049211/1‑150 (MQ=255)
                                                                                                        tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTCGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAg                                               >  2:399052/1‑150 (MQ=255)
                                                                                                           cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACTCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcgc                                            <  2:1056990/150‑1 (MQ=255)
                                                                                                           cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATGTTTGGTTTTATGGAAgcgc                                            <  2:220039/150‑1 (MQ=255)
                                                                                                               cGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt                                        >  2:1267048/1‑150 (MQ=255)
                                                                                                                  tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                     <  2:1105260/150‑1 (MQ=255)
                                                                                                                  tGACAAATTGGCAATATGATAACATCCGTTGCCAGAGTGGCAACGGATGAATTTATATGTGTGACGTAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGAAAGTGATTTTTTTTTTTATGTAAGATCGATTCCa                                     >  1:1243874/1‑150 (MQ=255)
                                                                                                                   gACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAg                                    >  1:999815/1‑150 (MQ=255)
                                                                                                                         ttGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg                              <  1:670923/150‑1 (MQ=255)
                                                                                                                          tGGCAATATGATAACTTCCGTTGCCAGCTTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTggg                             <  2:1631273/150‑1 (MQ=255)
                                                                                                                            gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                           <  2:327181/150‑1 (MQ=255)
                                                                                                                            gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                           <  2:388299/150‑1 (MQ=255)
                                                                                                                             cAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAg                          <  1:16274/150‑1 (MQ=255)
                                                                                                                              aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGCGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                         <  1:491997/150‑1 (MQ=255)
                                                                                                                              aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                         <  2:1445607/150‑1 (MQ=255)
                                                                                                                                  tGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                     >  1:446632/1‑150 (MQ=255)
                                                                                                                                  tGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                     >  2:1866089/1‑150 (MQ=255)
                                                                                                                                    aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc                   <  2:295822/150‑1 (MQ=255)
                                                                                                                                    aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc                   >  2:1522418/1‑150 (MQ=255)
                                                                                                                                     tAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACTGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGTAGCGCGATTCCAGAACTGGGCAGACATTGcc                  <  2:562518/150‑1 (MQ=255)
                                                                                                                                       tcATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCgg                >  1:608190/2‑150 (MQ=255)
                                                                                                                                        cATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa               >  1:1737061/1‑150 (MQ=255)
                                                                                                                                          tCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  1:1064315/1‑150 (MQ=255)
                                                                                                                                          tCCGGTGCCAGATAGGCAACGGAAGAATTTATCTGTGTGACGGACGAATACCCGTAGAAAAACGCTAAACAAGAGAATGTAGACGATCGTGACTGGGATTTTTTGATTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATg             <  2:226650/150‑1 (MQ=255)
                                                                                                                                           ccGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGGATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  2:1240269/1‑150 (MQ=255)
                                                                                                                                           ccGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGGGAGAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  2:791905/1‑150 (MQ=255)
                                                                                                                                              ttGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGAc         <  1:1322173/150‑1 (MQ=255)
                                                                                                                                               tGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACg        <  2:275429/150‑1 (MQ=255)
                                                                                                                                                gCCAGATTGTCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGc       <  1:521707/150‑1 (MQ=255)
                                                                                                                                                   aGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTg    >  1:1444408/1‑150 (MQ=255)
                                                                                                                                                    gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGc   >  1:392856/1‑150 (MQ=255)
                                                                                                                                                     aTTGTCAACGGATGAATTTATCTGTGGCACCGAAGAAAAAACGTAGAAAAACCCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGcc  <  2:1388161/150‑1 (MQ=255)
                                                                                                                                                     aTTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAACCGTAGAAAAACGCTAAACAAGAGAATGTAGCCGATGGGGACAGGGATTTTTTGTTTTATGGAAGCGCGATTACAGAACTGGGCCGACATTGCCGGATGCGACGCTTGcc  >  2:1870910/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCC  >  NC_000913/3234358‑3234656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: