Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,234,508 T→C 5.9% intergenic (‑53/+231) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,5080TC 5.9% 353.8 / 9.1 120intergenic (‑53/+231)higB/rlmGmRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (48/63);  new base C (5/2);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 122 total aligned reads displayed.

CTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCC  >  NC_000913/3234360‑3234656
                                                                                                                                                    |                                                                                                                                                    
cTTAGCAATCGTGTTCCCCAGAGCCACCATCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAtt                                                                                                                                                     <  1:607943/150‑1 (MQ=255)
 ttAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTg                                                                                                                                                    <  1:491474/150‑1 (MQ=255)
   cgCAAGCGTGTTCCCCAGCGCCCCCAACTCCGTTTTATGTTGCGGGTATTTTTCCGGAGCATCTTTCAATGCTGTTTGAGTAATCAGGTGCATTCTTCATCACGTCCGGGGACATATTGGCAATATGATAACATCCGTGGCCAGATTGGc                                                                                                                                                  <  2:130368/149‑1 (MQ=255)
   atcAATCGTGTTCCCCAGAGCCACCAACTCCGTTTAATGTTGCGGGTATTTATCCGCAGCATCTTTCAATGCTTTTTGAGTAATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                  <  1:1684157/148‑1 (MQ=255)
   aGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCAATCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc                                                                                                                                                  <  1:1676764/150‑1 (MQ=255)
        tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACgg                                                                                                                                             <  2:788686/150‑1 (MQ=255)
         ggtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTTCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCTCGTCCGTTGACTAATTGGCAATATGATAACATCCGTTTCCAGATTGGCAACgta                                                                                                                                            <  2:502071/149‑3 (MQ=255)
         cGTGTTCCGCTGAGCCACCTACTGCGTTTTATGTTGCGGGTATTTTTGCGCAGCATCTATCATTCCTTTTTGAGTTTTCATGTGCATTGTTCATCATGTCCGATGACAAATTGGCATTATGATAACATCCGTTGCCAATCTGGCAAGGGa                                                                                                                                            <  2:1839614/150‑1 (MQ=255)
          gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                           >  2:37105/1‑150 (MQ=255)
          gtgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGAt                                                                                                                                           >  1:1331752/1‑150 (MQ=255)
           tgtTCCCCAGAGCCACCAACTCCGTTTTAAGTTGCGGGTATTTTACCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCAATCTTCATCCCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATCTGGCAACGGATg                                                                                                                                          <  1:55981/150‑1 (MQ=255)
             ttCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCTGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGaa                                                                                                                                        <  2:1063758/150‑1 (MQ=255)
                cccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACATATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt                                                                                                                                     >  1:295822/1‑150 (MQ=255)
                 ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                    >  1:1070734/1‑150 (MQ=255)
                 ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTATTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTa                                                                                                                                    >  2:521707/1‑150 (MQ=255)
                   agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCTCAGCATCTTTCATTTCTTTTTGAGTTATCATGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCTGTTTGGCAACGGATGAATTTATc                                                                                                                                  <  2:1683407/150‑1 (MQ=255)
                   agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATc                                                                                                                                  <  2:280925/150‑1 (MQ=255)
                    gagCCACAAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCt                                                                                                                                 <  2:1012529/150‑1 (MQ=255)
                     cgCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtg                                                                                                                                <  2:1331729/149‑1 (MQ=255)
                      gCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATAACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgt                                                                                                                               >  1:904890/1‑150 (MQ=255)
                       ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtg                                                                                                                              <  1:1291335/150‑1 (MQ=255)
                               tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaa                                                                                                                      <  2:1525589/150‑1 (MQ=255)
                                ccGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATGACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                     <  1:595738/150‑1 (MQ=255)
                                ccGTTTTATGTTGAGGGTATTTTTCCGCAGCATATTTCAATGCTTTATGAGTTAGCAGGTGCATTCTGCATCACGTCCGTGGACAAATTGGCAATATGATAACATCCGTTGCAAGATTGGCAACGGATGAATTTATCTGTGTGACGgaag                                                                                                                     <  1:154048/150‑1 (MQ=255)
                                  gTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCACTGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGAAGgaagaa                                                                                                                   >  1:98848/1‑150 (MQ=255)
                                   ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGTTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt                                                                                                                  <  1:228132/150‑1 (MQ=255)
                                   ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt                                                                                                                  <  2:646875/150‑1 (MQ=255)
                                            gCGGGTATTTTTCCGCAGCATCTTTCAATGCGTTTTGAGTTAACAGGTGCATTCTACATCACGTCCGTTGAATAATTGGCAATATTATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATACACCTAGa                                                                                                         <  2:843525/150‑1 (MQ=255)
                                               ggTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaa                                                                                                      >  2:1682039/1‑150 (MQ=255)
                                                 tATTTTTCCGCAGCATCTTTCAATGCTTTTTGATATATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTTGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc                                                                                                    <  1:1234603/150‑1 (MQ=255)
                                                   tttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGATGGAAGAATAAACGTAGAAAAACGc                                                                                                  <  1:1306613/150‑1 (MQ=255)
                                                    ttttCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCt                                                                                                 >  1:536983/1‑150 (MQ=255)
                                                           cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAACCGCTAAACAag                                                                                          >  1:1450088/1‑150 (MQ=255)
                                                           cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                          <  2:1248739/150‑1 (MQ=255)
                                                           cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                          <  2:519296/150‑1 (MQ=255)
                                                           cagcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag                                                                                          <  2:616718/150‑1 (MQ=255)
                                                            agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga                                                                                         <  2:1275095/150‑1 (MQ=255)
                                                            agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga                                                                                         <  2:381646/150‑1 (MQ=255)
                                                              caTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAgaga                                                                                       <  2:861667/150‑1 (MQ=255)
                                                                tCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAAt                                                                                     >  1:327181/1‑150 (MQ=255)
                                                                  tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt                                                                                   >  1:1696777/1‑150 (MQ=255)
                                                                  tttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAAATGGCAATATGATAACATCCTTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAACAATAAACGTAGAAAAACGCAAAACAATAGAATGt                                                                                   >  1:345174/1‑150 (MQ=255)
                                                                   ttCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCCATGACAAATTGGCAATATGATAACATCCGTAGCAAGATTGGCAACGGATGAATTTATATGTGTGACGGAAGAAAAAACGTAGAAAAACTCTAAACAATAGAATGTa                                                                                  >  1:274241/1‑150 (MQ=255)
                                                                     cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                                <  1:1872786/150‑1 (MQ=255)
                                                                     cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGa                                                                                >  2:806695/1‑150 (MQ=255)
                                                                        tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGa                                                                             >  1:190118/1‑150 (MQ=255)
                                                                        tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGATAAACAAGAGAATGTAGACGa                                                                             >  2:1723594/1‑150 (MQ=255)
                                                                           ttttttAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  2:181901/150‑1 (MQ=255)
                                                                           tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  2:870114/150‑1 (MQ=255)
                                                                           tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          >  2:140911/1‑150 (MQ=255)
                                                                           attttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATgg                                                                          <  1:240247/149‑1 (MQ=255)
                                                                            ttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGt                                                                         <  1:71503/150‑1 (MQ=255)
                                                                              ttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGa                                                                       >  1:1445607/1‑150 (MQ=255)
                                                                                 aGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAACCGCTAAACAAGAGAATGTAGACGATGGTGACAg                                                                    >  2:280006/1‑150 (MQ=255)
                                                                                  gTTATCAGGGGCATTCAGCATCACGTCCGTTGACAAATAGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAgg                                                                   <  2:904890/150‑1 (MQ=255)
                                                                                    tATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGa                                                                 >  1:746784/1‑150 (MQ=255)
                                                                                       cAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttt                                                              >  1:571794/1‑150 (MQ=255)
                                                                                         ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt                                                            <  2:1331752/150‑1 (MQ=255)
                                                                                         ggTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt                                                            >  2:1692961/1‑150 (MQ=255)
                                                                                          gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt                                                           <  2:602285/150‑1 (MQ=255)
                                                                                           tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTg                                                          <  1:702270/150‑1 (MQ=255)
                                                                                            gCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt                                                         >  1:123731/1‑150 (MQ=255)
                                                                                             cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt                                                        >  2:546579/1‑150 (MQ=255)
                                                                                             cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt                                                        <  2:1524733/150‑1 (MQ=255)
                                                                                                tcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                     <  1:854698/150‑1 (MQ=255)
                                                                                                tcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa                                                     >  2:1516988/1‑150 (MQ=255)
                                                                                                      tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTCGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAg                                               >  2:399052/1‑150 (MQ=255)
                                                                                                         cGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACTCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAgcgc                                            <  2:1056990/150‑1 (MQ=255)
                                                                                                           tCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGa                                          >  2:1859502/1‑150 (MQ=255)
                                                                                                             cGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt                                        >  2:1267048/1‑150 (MQ=255)
                                                                                                                tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTACACAAGAGAATGTAGAAGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                     <  2:46016/150‑1 (MQ=255)
                                                                                                                tGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa                                     <  2:1105260/150‑1 (MQ=255)
                                                                                                                tGACAAATTGGCAATATGATAACATCCGTTGCCAGAGTGGCAACGGATGAATTTATATGTGTGACGTAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGAAAGTGATTTTTTTTTTTATGTAAGATCGATTCCa                                     >  1:1243874/1‑150 (MQ=255)
                                                                                                                 gACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAGCAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAg                                    >  2:260939/1‑150 (MQ=255)
                                                                                                                 gACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAg                                    >  1:999815/1‑150 (MQ=255)
                                                                                                                   cAAATTGGCAATATGATAACATCCGTTGCCAGAGTGGCTACGGATGAATTTATCTGTGTGACGGAAGAATAAACGAAGAAAAACGCTAAACAAGAGAATGTAAAAGATGGTGACAGAGATTTTTTGTTTAATGGAAGCGCGAATCCAGaa                                  >  2:112349/1‑150 (MQ=255)
                                                                                                                       ttGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg                              <  1:670923/150‑1 (MQ=255)
                                                                                                                          gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                           <  2:327181/150‑1 (MQ=255)
                                                                                                                          gCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCa                           <  2:388299/150‑1 (MQ=255)
                                                                                                                           cAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAg                          <  1:16274/150‑1 (MQ=255)
                                                                                                                            aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGCGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                         <  1:491997/150‑1 (MQ=255)
                                                                                                                            aaTATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGa                         <  2:1445607/150‑1 (MQ=255)
                                                                                                                                tGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt                     >  2:1866089/1‑150 (MQ=255)
                                                                                                                                  aTAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc                   >  2:1522418/1‑150 (MQ=255)
                                                                                                                                   tAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACTGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGTAGCGCGATTCCAGAACTGGGCAGACATTGcc                  <  2:562518/150‑1 (MQ=255)
                                                                                                                                     tcATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCgg                >  1:608190/2‑150 (MQ=255)
                                                                                                                                      cATCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGa               >  1:1737061/1‑150 (MQ=255)
                                                                                                                                       aTCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTTCCGGAt              >  1:1570725/1‑150 (MQ=255)
                                                                                                                                        tCCGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  1:1064315/1‑150 (MQ=255)
                                                                                                                                        tCCGGTGCCAGATAGGCAACGGAAGAATTTATCTGTGTGACGGACGAATACCCGTAGAAAAACGCTAAACAAGAGAATGTAGACGATCGTGACTGGGATTTTTTGATTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATg             <  2:226650/150‑1 (MQ=255)
                                                                                                                                         ccGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGGATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  2:1240269/1‑150 (MQ=255)
                                                                                                                                         ccGTTGCCAATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGGGAGAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGc            >  2:791905/1‑150 (MQ=255)
                                                                                                                                            ttGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGAc         <  1:1322173/150‑1 (MQ=255)
                                                                                                                                             tGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACg        <  2:275429/150‑1 (MQ=255)
                                                                                                                                              gCCAGATTGTCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGc       <  1:521707/150‑1 (MQ=255)
                                                                                                                                                  gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGc   >  1:392856/1‑150 (MQ=255)
                                                                                                                                                  gATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGc   <  1:13743/150‑1 (MQ=255)
                                                                                                                                                   aTTGTCAACGGATGAATTTATCTGTGGCACCGAAGAAAAAACGTAGAAAAACCCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGcc  <  2:1388161/150‑1 (MQ=255)
                                                                                                                                                   aTTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAACCGTAGAAAAACGCTAAACAAGAGAATGTAGCCGATGGGGACAGGGATTTTTTGTTTTATGGAAGCGCGATTACAGAACTGGGCCGACATTGCCGGATGCGACGCTTGcc  >  2:1870910/1‑150 (MQ=255)
                                                                                                                                                   aTTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGCCGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGcc  >  2:542851/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCC  >  NC_000913/3234360‑3234656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: