Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,441 T→G 7.7% T71P (ACG→CCG)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4410TG 7.7% 292.0 / 9.1 121T71P (ACG→CCG) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (56/53);  new base G (7/3);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTT  >  NC_000913/3256293‑3256589
                                                                                                                                                    |                                                                                                                                                    
aTCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTAAGCACTTCCAGCCCGGCGTTGGCATTACCCCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCACACCATTCCCGTg                                                                                                                                                     >  1:1261555/1‑150 (MQ=255)
  cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                   >  2:1409394/1‑150 (MQ=255)
    tAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                 >  1:868898/1‑150 (MQ=255)
     aaCGGCGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGGAGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATGTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCggg                                                                                                                                                <  1:468500/150‑1 (MQ=255)
      aCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgggc                                                                                                                                               >  1:461015/1‑149 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCATTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAAc                                                                                                                                             >  1:704863/1‑150 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                             <  1:1502014/150‑1 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                             <  1:457277/150‑1 (MQ=255)
         gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCGCCAGCGCCGCCGCTATCGGCAGCCCCACCATTCCCGTGGCGGGAACg                                                                                                                                            >  1:107306/1‑150 (MQ=255)
           gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                          <  2:612523/150‑1 (MQ=255)
            ccTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                         <  1:1556032/149‑1 (MQ=255)
            aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                         <  2:752241/150‑1 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCCGCCCCACCATTCCCGTGCCGGGACCGGTGACg                                                                                                                                      >  1:533308/1‑150 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACACTTCCCGTGCCGGGCACGGTGACg                                                                                                                                      >  2:1776943/1‑150 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATAGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCg                                                                                                                                      >  1:1359565/1‑150 (MQ=255)
               ttCCCCGCCGCCAGCAGAGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                      <  2:113754/150‑1 (MQ=255)
               ctCCCCGCCGCCAGCCGTGCTGAGGCCTCCCCCACTGCCTGCGCTGTTCCGGCTACCACCACTTCCACCCCGGCGTAGGCATCACCCCCTCACGCCCCCAGCGCCGCCGCAATCCGCAGCCCCACCATTCCCGAGCCGCGAACGGTGACg                                                                                                                                      <  2:707220/149‑1 (MQ=255)
                tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                     <  2:1862212/150‑5 (MQ=255)
                 ccccGCCGCCAGCAGTGCGTTTGCATCGGCAATTGCCGGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggct                                                                                                                                    <  2:52088/150‑6 (MQ=255)
                  cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                   <  2:1043956/150‑1 (MQ=255)
                        gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                             >  1:1284836/1‑150 (MQ=255)
                          cagcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAGCGGAGCCGCCCAGACCggt                                                                                                                           >  2:172706/1‑148 (MQ=255)
                           agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                          >  2:766849/1‑150 (MQ=255)
                           agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGCCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                          <  2:1165953/150‑1 (MQ=255)
                              agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGACCGgtcttc                                                                                                                       >  2:845757/1‑150 (MQ=255)
                              agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                       <  1:1436575/150‑1 (MQ=255)
                                       gCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt                                                                                                              >  1:517400/1‑150 (MQ=255)
                                          tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                           <  1:660515/150‑1 (MQ=255)
                                           cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                          <  1:616287/150‑1 (MQ=255)
                                            ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCgc                                                                                                         >  2:1846797/1‑149 (MQ=255)
                                               aaTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAc                                                                                                      >  1:525062/1‑150 (MQ=255)
                                                   gCCTGCGCTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                                  >  2:472669/1‑150 (MQ=255)
                                                    ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                                                                 <  2:979421/150‑1 (MQ=255)
                                                     cTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                                <  2:789463/150‑1 (MQ=255)
                                                        cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                             <  2:715769/150‑1 (MQ=255)
                                                          cTGTTGCGTCTTTCAGCACTACCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                           <  1:540485/150‑1 (MQ=255)
                                                           tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                          <  2:631992/150‑1 (MQ=255)
                                                           tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                          <  2:321182/150‑1 (MQ=255)
                                                           ggTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATATCCACCTAACGACCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                          <  2:337094/149‑1 (MQ=255)
                                                            gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                                                                         <  2:1863928/150‑1 (MQ=255)
                                                              tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGCTTTGGCGAAACCCAGGCTTCTACACg                                                                                       >  2:1729021/1‑150 (MQ=255)
                                                               gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAGTCGGCCGCCCCACCATTCCCGGGCCGGGAGCCGTGACGCCCTGCCCGGTCTTCATCAGCTTTGGGGAAGCCCAGGCTTCTCCACGt                                                                                      >  2:1188090/1‑150 (MQ=255)
                                                                cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                     <  1:81352/150‑1 (MQ=255)
                                                                cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCAGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                     >  1:34721/1‑150 (MQ=255)
                                                                 gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                    <  2:843193/150‑1 (MQ=255)
                                                                  tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCa                                                                                   <  1:1409394/150‑1 (MQ=255)
                                                                     ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCGAACGCCCCCAGCGCCGCCGCAAGCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGGTCTTGATCAGATGTGGCGATACCCAGGCGTCTCCCCGGTCAccc                                                                                >  1:127328/1‑150 (MQ=255)
                                                                       cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTCCACGTTCAACCgg                                                                              >  1:1679079/1‑150 (MQ=255)
                                                                       cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                              >  2:635084/1‑150 (MQ=255)
                                                                       cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                              <  2:1670585/150‑1 (MQ=255)
                                                                        aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCggc                                                                             >  2:832170/1‑149 (MQ=255)
                                                                        aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCggc                                                                             >  2:1063431/1‑149 (MQ=255)
                                                                        aGCACTTCCAGCCAGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                             <  1:420575/150‑1 (MQ=255)
                                                                         gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTGTTCCTCAGATTTGGCGAAACCCAGGCTTCTACCCGTTCAACCGGAc                                                                            >  1:1635936/1‑150 (MQ=255)
                                                                         gCACTTCCAGCCCGGCGGTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTGCTACACGTTCAACCGGAc                                                                            >  1:946420/1‑150 (MQ=255)
                                                                          cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGACGCCCAGCCCGGTCTTCATCAGATTTGGCGCGACCCAGGCTGCGACACGTTCCACCGGAcc                                                                           >  1:227446/1‑150 (MQ=255)
                                                                            cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCtt                                                                         >  2:446462/1‑150 (MQ=255)
                                                                                 aGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCCTCAGATTGGGCGAAACCCAGGCGGCTACCCGTTCCACCGGCCCTACCAGt                                                                    >  2:827590/1‑150 (MQ=255)
                                                                                  gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCTTGCCGGGAACGGTGACGCCCAGACCGTTCTACATCAGATTTGGCGAAGCCCAGGCTTCTACACGTTCAACCGGCCCTACCAgat                                                                   >  1:1636652/1‑148 (MQ=255)
                                                                                     cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                <  1:1829208/150‑1 (MQ=255)
                                                                                     cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                <  2:1731598/150‑1 (MQ=255)
                                                                                       gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                              <  2:1308697/150‑1 (MQ=255)
                                                                                       gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                              <  2:451896/150‑1 (MQ=255)
                                                                                        cggtGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCATCCGGACCTTCCAGTTCTGCCg                                                             <  2:523625/147‑1 (MQ=255)
                                                                                          ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgca                                                           <  2:1047555/150‑1 (MQ=255)
                                                                                           tGGCATTTCAACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                          <  2:832584/150‑1 (MQ=255)
                                                                                                   cccccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCCGGCTTCTACACGTTCAACCGGCCCTTCCAGTTCTGCCGCAGCAAccgcc                                                  >  2:672119/4‑150 (MQ=255)
                                                                                                    cACCTAACGCCCCCAGCGCCGCCGCAATCGGAAGCCCCACCATTCCCGTGCCGGGAACGCTGACGCCCAGACCGTTCTTCAACAGATTACGCTAAACCCACGCGTCTACACGTTAAAACGGACCGTCCAGATCTGCCGCAGCAAccgccg                                                 >  2:33797/1‑150 (MQ=255)
                                                                                                     aCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGACCGTTCTTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCACccgccgc                                                >  2:393880/1‑150 (MQ=255)
                                                                                                      ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTAAACGTTCAACCGGACCTTCCAGTTAAGCCGCAGCAAccgccgcc                                               >  2:170302/1‑150 (MQ=255)
                                                                                                       cTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgccg                                              <  2:411980/150‑1 (MQ=255)
                                                                                                        tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTTCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAGCCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                             >  1:379/1‑150 (MQ=255)
                                                                                                         aaCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCgccgccgcc                                            <  2:383374/150‑1 (MQ=255)
                                                                                                            gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                         >  2:1467554/1‑150 (MQ=255)
                                                                                                             cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                        >  2:535399/1‑150 (MQ=255)
                                                                                                             cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                        >  1:674137/1‑150 (MQ=255)
                                                                                                               cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                      <  2:1858495/150‑1 (MQ=255)
                                                                                                                       cgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGTCCTTCCAGTTCTGGCGCAGCAACCGCCGCCGCCAGCGCGAGTGAAAt                              >  2:754126/1‑150 (MQ=255)
                                                                                                                           gcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGt                          <  1:1636308/150‑1 (MQ=255)
                                                                                                                             aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAGCCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCAGGGACATCGGTTc                        >  1:918601/1‑150 (MQ=255)
                                                                                                                              aTCTGCAGCCCGAACATTCCCGTGACTGGAACGGTGACTCCCAGACGGTTCTTCAAGAGTTTTGGCGAAACCCAGGCTTCTACACGTTCAACCTGATCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCa                       <  1:301416/150‑1 (MQ=255)
                                                                                                                                 ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTAAGCCGCAGCAACCGCCGCCGCCAGCGCCAGAGAAAACGGTTCAGTa                    >  2:1763988/1‑150 (MQ=255)
                                                                                                                                 ggCAGCCCCAACATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTGCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTa                    >  1:1227323/1‑150 (MQ=255)
                                                                                                                                  gCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTAc                   >  2:1306282/1‑150 (MQ=255)
                                                                                                                                     gCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCAGTGAAATCGGGTCAGTACATc                >  1:667389/1‑150 (MQ=255)
                                                                                                                                      cgtcaccgTTCCCGGCACGGGAATGGTGGCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGGCAGCGCCAGTGAAATCGGTTCAGTACATcc               >  1:1606971/4‑150 (MQ=255)
                                                                                                                                      ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACCTcc               >  2:1232468/1‑150 (MQ=255)
                                                                                                                                        ccaccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCGGCGCCAGTGAAATCGGTTCAGTACATcccc             >  1:472698/1‑149 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACGTCCCAg            >  2:370791/1‑150 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg            >  1:1781234/1‑150 (MQ=255)
                                                                                                                                          accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc           >  1:229856/1‑150 (MQ=255)
                                                                                                                                          accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc           >  2:1066377/1‑150 (MQ=255)
                                                                                                                                          accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc           >  1:430506/1‑150 (MQ=255)
                                                                                                                                             aTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc        <  2:986535/150‑1 (MQ=255)
                                                                                                                                             aTTCCCGTGCCGCGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCTGCTGCAAGCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc        <  2:1866609/150‑1 (MQ=255)
                                                                                                                                              ttCCCGTGCCGGGACCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCCGTGAAATCGGTTCAGTACATCCCCGCGCCg       >  2:1315159/1‑150 (MQ=255)
                                                                                                                                               tCCCGCGCCGGGAACGGTGACGCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAACGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCgg      <  2:670327/150‑1 (MQ=255)
                                                                                                                                                 ccgggccgggAACGGTGACGCCCAGCGCGGTCTTCATCAGATTTGGCGACACCCATGCTTCGAAACGTTCAACCGGAGCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGAGACAGTGAAATCGGTTAAGTACATCCCAGCGCCTGtt    <  2:317715/150‑1 (MQ=255)
                                                                                                                                                   gTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt  <  2:34721/150‑1 (MQ=255)
                                                                                                                                                   gTGCCGGGAAAGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAAACCAGTATTCTACACGGTCAACCGGTCCTTCCTGTGCTGCCGCAGCTACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt  <  2:107306/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTT  >  NC_000913/3256293‑3256589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: