Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,443 C→A 5.7% G70V (GGC→GTC) ‡ yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4430CA 5.7% 334.8 / 4.4 122G70V (GGC→GTC) ‡yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (56/57);  new base A (4/4);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

CTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256295‑3256592
                                                                                                                                                    |                                                                                                                                                     
cTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                                                                      >  2:1409394/1‑150 (MQ=255)
    aCGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgggc                                                                                                                                                  >  1:461015/1‑149 (MQ=255)
      ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCATTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAAc                                                                                                                                                >  1:704863/1‑150 (MQ=255)
      ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                                <  1:457277/150‑1 (MQ=255)
       gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCGCCAGCGCCGCCGCTATCGGCAGCCCCACCATTCCCGTGGCGGGAACg                                                                                                                                               >  1:107306/1‑150 (MQ=255)
          ccTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                            <  1:1556032/149‑1 (MQ=255)
          aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                            <  2:752241/150‑1 (MQ=255)
           cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                           <  2:611373/150‑2 (MQ=255)
             ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACACTTCCCGTGCCGGGCACGGTGACg                                                                                                                                         >  2:1776943/1‑150 (MQ=255)
             ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATAGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCg                                                                                                                                         >  1:1359565/1‑150 (MQ=255)
             ttCCCCGCCGCCAGCAGAGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                         <  2:113754/150‑1 (MQ=255)
             ctCCCCGCCGCCAGCCGTGCTGAGGCCTCCCCCACTGCCTGCGCTGTTCCGGCTACCACCACTTCCACCCCGGCGTAGGCATCACCCCCTCACGCCCCCAGCGCCGCCGCAATCCGCAGCCCCACCATTCCCGAGCCGCGAACGGTGACg                                                                                                                                         <  2:707220/149‑1 (MQ=255)
              tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                        <  2:1862212/150‑5 (MQ=255)
               ccccGCCGCCAGCAGTGCGTTTGCATCGGCAATTGCCGGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggct                                                                                                                                       <  2:52088/150‑6 (MQ=255)
                cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCTGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                      >  2:835277/1‑150 (MQ=255)
                      gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                                >  1:1284836/1‑150 (MQ=255)
                        cagcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAGCGGAGCCGCCCAGACCggt                                                                                                                              >  2:172706/1‑148 (MQ=255)
                         agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                             >  2:766849/1‑150 (MQ=255)
                         agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGCCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                             <  2:1165953/150‑1 (MQ=255)
                          gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                            <  1:497505/150‑1 (MQ=255)
                            agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGACCGgtcttc                                                                                                                          >  2:845757/1‑150 (MQ=255)
                            agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                          <  1:1436575/150‑1 (MQ=255)
                             gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtca                                                                                                                         <  2:333418/150‑1 (MQ=255)
                                cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatca                                                                                                                      <  1:1599565/150‑1 (MQ=255)
                                     gCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt                                                                                                                 >  1:517400/1‑150 (MQ=255)
                                        tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                              <  1:660515/150‑1 (MQ=255)
                                         cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                             <  1:616287/150‑1 (MQ=255)
                                          ggCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCgc                                                                                                            >  2:1846797/1‑149 (MQ=255)
                                             aaTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAc                                                                                                         >  1:525062/1‑150 (MQ=255)
                                                 gCCTGCGCTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                                     >  2:472669/1‑150 (MQ=255)
                                                  ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                                                                    <  2:979421/150‑1 (MQ=255)
                                                   cTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                                   <  2:789463/150‑1 (MQ=255)
                                                      cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                                <  2:715769/150‑1 (MQ=255)
                                                        cTGTTGCGTCTTTCAGCACTACCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                              <  1:540485/150‑1 (MQ=255)
                                                         tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                             <  2:631992/150‑1 (MQ=255)
                                                         ggTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATATCCACCTAACGACCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                             <  2:337094/149‑1 (MQ=255)
                                                          gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                                                                            <  2:1863928/150‑1 (MQ=255)
                                                            tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGCTTTGGCGAAACCCAGGCTTCTACACg                                                                                          >  2:1729021/1‑150 (MQ=255)
                                                             gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAGTCGGCCGCCCCACCATTCCCGGGCCGGGAGCCGTGACGCCCTGCCCGGTCTTCATCAGCTTTGGGGAAGCCCAGGCTTCTCCACGt                                                                                         >  2:1188090/1‑150 (MQ=255)
                                                              cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                        <  1:81352/150‑1 (MQ=255)
                                                              cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCAGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                        >  1:34721/1‑150 (MQ=255)
                                                               gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                       <  2:843193/150‑1 (MQ=255)
                                                                tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCa                                                                                      <  1:1409394/150‑1 (MQ=255)
                                                                   ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCGAACGCCCCCAGCGCCGCCGCAAGCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGGTCTTGATCAGATGTGGCGATACCCAGGCGTCTCCCCGGTCAccc                                                                                   >  1:127328/1‑150 (MQ=255)
                                                                    tCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCGTCAGCCGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCg                                                                                  >  1:738111/1‑150 (MQ=255)
                                                                     cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                 <  1:959833/150‑1 (MQ=255)
                                                                     cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                 >  2:635084/1‑150 (MQ=255)
                                                                     cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                 <  2:1670585/150‑1 (MQ=255)
                                                                      aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCggc                                                                                >  2:1063431/1‑149 (MQ=255)
                                                                      aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                <  2:379214/150‑1 (MQ=255)
                                                                      aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                >  2:1743413/1‑150 (MQ=255)
                                                                      aGCACTTCCAGCCAGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                <  1:420575/150‑1 (MQ=255)
                                                                       gCACTTCCAGCCCGGCGGTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTGCTACACGTTCAACCGGAc                                                                               >  1:946420/1‑150 (MQ=255)
                                                                        cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGACGCCCAGCCCGGTCTTCATCAGATTTGGCGCGACCCAGGCTGCGACACGTTCCACCGGAcc                                                                              >  1:227446/1‑150 (MQ=255)
                                                                            tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCCTTCCCGTGCCGGGAACGGTGACGCACATACCGTTCTACATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                          <  2:76591/150‑1 (MQ=255)
                                                                               aGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCCTCAGATTGGGCGAAACCCAGGCGGCTACCCGTTCCACCGGCCCTACCAGt                                                                       >  2:827590/1‑150 (MQ=255)
                                                                                gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCTTGCCGGGAACGGTGACGCCCAGACCGTTCTACATCAGATTTGGCGAAGCCCAGGCTTCTACACGTTCAACCGGCCCTACCAgat                                                                      >  1:1636652/1‑148 (MQ=255)
                                                                                 cccGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTc                                                                     <  1:55278/150‑1 (MQ=255)
                                                                                   cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                   <  1:1829208/150‑1 (MQ=255)
                                                                                   cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                   <  2:1731598/150‑1 (MQ=255)
                                                                                     gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                                 <  2:1308697/150‑1 (MQ=255)
                                                                                     gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc                                                                 <  2:451896/150‑1 (MQ=255)
                                                                                      cggtGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCATCCGGACCTTCCAGTTCTGCCg                                                                <  2:523625/147‑1 (MQ=255)
                                                                                        ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgca                                                              <  2:1047555/150‑1 (MQ=255)
                                                                                         tGGCATTTCAACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                             <  2:832584/150‑1 (MQ=255)
                                                                                            cATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCGGGGATCGGTGACGCCCAGACCGTTCTTCATGAGATTTGGCGTCACCCAGGCGTCTAGCCGTTCACCCGGCCGTTCCAGTTCTGCCGCAGCaa                                                          >  2:1636790/1‑150 (MQ=255)
                                                                                                 cccccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCCGGCTTCTACACGTTCAACCGGCCCTTCCAGTTCTGCCGCAGCAAccgcc                                                     >  2:672119/4‑150 (MQ=255)
                                                                                                  cACCTAACGCCCCCAGCGCCGCCGCAATCGGAAGCCCCACCATTCCCGTGCCGGGAACGCTGACGCCCAGACCGTTCTTCAACAGATTACGCTAAACCCACGCGTCTACACGTTAAAACGGACCGTCCAGATCTGCCGCAGCAAccgccg                                                    >  2:33797/1‑150 (MQ=255)
                                                                                                   aCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGACCGTTCTTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCACccgccgc                                                   >  2:393880/1‑150 (MQ=255)
                                                                                                     cTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgccg                                                 <  2:411980/150‑1 (MQ=255)
                                                                                                      tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTTCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAGCCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                >  1:379/1‑150 (MQ=255)
                                                                                                       aaCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCgccgccgcc                                               <  2:383374/150‑1 (MQ=255)
                                                                                                          gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                            >  2:1467554/1‑150 (MQ=255)
                                                                                                           cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                           >  1:674137/1‑150 (MQ=255)
                                                                                                           cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                           >  2:535399/1‑150 (MQ=255)
                                                                                                             cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                         <  2:1858495/150‑1 (MQ=255)
                                                                                                                       ccgcAATCCGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                               <  1:1661255/150‑1 (MQ=255)
                                                                                                                        cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                              >  2:472470/1‑150 (MQ=255)
                                                                                                                         gcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGt                             <  1:1636308/150‑1 (MQ=255)
                                                                                                                           aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAGCCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCAGGGACATCGGTTc                           >  1:918601/1‑150 (MQ=255)
                                                                                                                            aTCTGCAGCCCGAACATTCCCGTGACTGGAACGGTGACTCCCAGACGGTTCTTCAAGAGTTTTGGCGAAACCCAGGCTTCTACACGTTCAACCTGATCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCa                          <  1:301416/150‑1 (MQ=255)
                                                                                                                               ggCAGCCCCAACATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTGCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTa                       >  1:1227323/1‑150 (MQ=255)
                                                                                                                                gCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTAc                      >  2:1306282/1‑150 (MQ=255)
                                                                                                                                    cgtcaccgTTCCCGGCACGGGAATGGTGGCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGGCAGCGCCAGTGAAATCGGTTCAGTACATcc                  >  1:1606971/4‑150 (MQ=255)
                                                                                                                                    ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACCTcc                  >  2:1232468/1‑150 (MQ=255)
                                                                                                                                      ccaccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCGGCGCCAGTGAAATCGGTTCAGTACATcccc                >  1:472698/1‑149 (MQ=255)
                                                                                                                                       caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg               >  1:1781234/1‑150 (MQ=255)
                                                                                                                                        accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc              >  1:229856/1‑150 (MQ=255)
                                                                                                                                        accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc              >  1:430506/1‑150 (MQ=255)
                                                                                                                                        accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc              >  2:1066377/1‑150 (MQ=255)
                                                                                                                                           aTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc           <  2:986535/150‑1 (MQ=255)
                                                                                                                                           aTTCCCGTGCCGCGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCTGCTGCAAGCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc           <  2:1866609/150‑1 (MQ=255)
                                                                                                                                             tCCCGCGCCGGGAACGGTGACGCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAACGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCgg         <  2:670327/150‑1 (MQ=255)
                                                                                                                                               ccgggccgggAACGGTGACGCCCAGCGCGGTCTTCATCAGATTTGGCGACACCCATGCTTCGAAACGTTCAACCGGAGCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGAGACAGTGAAATCGGTTAAGTACATCCCAGCGCCTGtt       <  2:317715/150‑1 (MQ=255)
                                                                                                                                                 gTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt     <  2:34721/150‑1 (MQ=255)
                                                                                                                                                 gTGCCGGGAAAGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAAACCAGTATTCTACACGGTCAACCGGTCCTTCCTGTGCTGCCGCAGCTACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt     <  2:107306/150‑1 (MQ=255)
                                                                                                                                                   gCCGGGTACGGGGACGCCCAGACCGTTATTCATCAGATTTGGTGAACCCCAGGCTTCTACACGATCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc   <  2:973489/150‑1 (MQ=255)
                                                                                                                                                    ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGACACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt  <  1:1373172/150‑1 (MQ=255)
                                                                                                                                                    ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt  <  2:1284836/150‑1 (MQ=255)
                                                                                                                                                    ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt  <  1:1618487/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256295‑3256592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: