Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,450 C→T 5.6% V68I (GTT→ATT)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4500CT 5.6% 371.8 / 8.0 125V68I (GTT→ATT) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (58/59);  new base T (4/3);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-02

Only 100 of 129 total aligned reads displayed.

GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCT  >  NC_000913/3256301‑3256599
                                                                                                                                                     |                                                                                                                                                     
ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCATTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAAc                                                                                                                                                       >  1:704863/1‑150 (MQ=255)
ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                                       <  1:1502014/150‑1 (MQ=255)
ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGGCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                                       <  1:457277/150‑1 (MQ=255)
 gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCGCCAGCGCCGCCGCTATCGGCAGCCCCACCATTCCCGTGGCGGGAACg                                                                                                                                                      >  1:107306/1‑150 (MQ=255)
   gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                                    <  2:612523/150‑1 (MQ=255)
    ccTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                                   <  1:1556032/149‑1 (MQ=255)
    aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTg                                                                                                                                                   <  2:752241/150‑1 (MQ=255)
     cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                                  <  2:611373/150‑2 (MQ=255)
       ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCCGCCCCACCATTCCCGTGCCGGGACCGGTGACg                                                                                                                                                >  1:533308/1‑150 (MQ=255)
       ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACACTTCCCGTGCCGGGCACGGTGACg                                                                                                                                                >  2:1776943/1‑150 (MQ=255)
       ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATAGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCg                                                                                                                                                >  1:1359565/1‑150 (MQ=255)
       ttCCCCGCCGCCAGCAGAGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACg                                                                                                                                                <  2:113754/150‑1 (MQ=255)
        tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                               <  2:1862212/150‑5 (MQ=255)
         ccccGCCGCCAGCAGTGCGTTTGCATCGGCAATTGCCGGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggct                                                                                                                                              <  2:52088/150‑6 (MQ=255)
          cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCTGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                             >  2:835277/1‑150 (MQ=255)
          cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                                             <  2:1043956/150‑1 (MQ=255)
                gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                                       >  1:1284836/1‑150 (MQ=255)
                  cagcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAGCGGAGCCGCCCAGACCggt                                                                                                                                     >  2:172706/1‑148 (MQ=255)
                   agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                                    >  2:766849/1‑150 (MQ=255)
                   agcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGCCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttc                                                                                                                                    <  2:1165953/150‑1 (MQ=255)
                      agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGACCGgtcttc                                                                                                                                 >  2:845757/1‑150 (MQ=255)
                      agTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                                 <  1:1436575/150‑1 (MQ=255)
                       gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtca                                                                                                                                <  2:333418/150‑1 (MQ=255)
                          cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatca                                                                                                                             <  1:1599565/150‑1 (MQ=255)
                               gCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAttt                                                                                                                        >  1:517400/1‑150 (MQ=255)
                                  tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                                     <  1:660515/150‑1 (MQ=255)
                                   cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                                    <  1:616287/150‑1 (MQ=255)
                                       aaTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAc                                                                                                                >  1:525062/1‑150 (MQ=255)
                                        aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAcc                                                                                                               <  2:693350/150‑1 (MQ=255)
                                           gCCTGCGCTGTTGCGTCTTTCAGCACTTCCCGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                                            >  2:472669/1‑150 (MQ=255)
                                            ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCAAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                                                                           <  2:979421/150‑1 (MQ=255)
                                             cTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                                          <  2:789463/150‑1 (MQ=255)
                                                cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                                       <  2:715769/150‑1 (MQ=255)
                                                  cTGTTGCGTCTTTCAGCACTACCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                                     <  1:540485/150‑1 (MQ=255)
                                                   tGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                                    <  2:321182/150‑1 (MQ=255)
                                                    gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                                                                                   <  2:1863928/150‑1 (MQ=255)
                                                       gCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAGTCGGCCGCCCCACCATTCCCGGGCCGGGAGCCGTGACGCCCTGCCCGGTCTTCATCAGCTTTGGGGAAGCCCAGGCTTCTCCACGt                                                                                                >  2:1188090/1‑150 (MQ=255)
                                                        cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                               <  1:81352/150‑1 (MQ=255)
                                                        cGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCAGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                               >  1:34721/1‑150 (MQ=255)
                                                         gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGCCGCCCAGCCCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTTCTACCCGTTc                                                                                              >  1:1345600/1‑150 (MQ=255)
                                                         gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                              <  2:843193/150‑1 (MQ=255)
                                                             ttCAGCACTTCCGGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc                                                                                          <  2:1276605/150‑1 (MQ=255)
                                                             ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCGAACGCCCCCAGCGCCGCCGCAAGCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGGTCTTGATCAGATGTGGCGATACCCAGGCGTCTCCCCGGTCAccc                                                                                          >  1:127328/1‑150 (MQ=255)
                                                              tCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCGTCAGCCGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCg                                                                                         >  1:738111/1‑150 (MQ=255)
                                                               cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTCCACGTTCAACCgg                                                                                        >  1:1679079/1‑150 (MQ=255)
                                                               cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                        <  2:1670585/150‑1 (MQ=255)
                                                               cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                        <  1:959833/150‑1 (MQ=255)
                                                               cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                                                                        >  2:635084/1‑150 (MQ=255)
                                                                aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCggc                                                                                       >  2:832170/1‑149 (MQ=255)
                                                                aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                       <  2:379214/150‑1 (MQ=255)
                                                                aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                       >  2:1743413/1‑150 (MQ=255)
                                                                 gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTGTTCCTCAGATTTGGCGAAACCCAGGCTTCTACCCGTTCAACCGGAc                                                                                      >  1:1635936/1‑150 (MQ=255)
                                                                 gCACTTCCAGCCCGGCGGTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCCTCAGATTTGGCGAAACCCAGGCTGCTACACGTTCAACCGGAc                                                                                      >  1:946420/1‑150 (MQ=255)
                                                                  cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGCACGGTGACGCCCAGCCCGGTCTTCATCAGATTTGGCGCGACCCAGGCTGCGACACGTTCCACCGGAcc                                                                                     >  1:227446/1‑150 (MQ=255)
                                                                   aCTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTGCATCAGATTTGGCGAAACCCAGGCTTCTACACGGTCAACCGGACCt                                                                                    >  1:261127/1‑150 (MQ=255)
                                                                    cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCtt                                                                                   >  2:446462/1‑150 (MQ=255)
                                                                         aGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCCTCAGATTGGGCGAAACCCAGGCGGCTACCCGTTCCACCGGCCCTACCAGt                                                                              >  2:827590/1‑150 (MQ=255)
                                                                             cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                          <  2:1731598/150‑1 (MQ=255)
                                                                             cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                          <  1:1829208/150‑1 (MQ=255)
                                                                                  ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgca                                                                     <  2:1047555/150‑1 (MQ=255)
                                                                                           cccccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCCGGCTTCTACACGTTCAACCGGCCCTTCCAGTTCTGCCGCAGCAAccgcc                                                            >  2:672119/4‑150 (MQ=255)
                                                                                            cACCTAACGCCCCCAGCGCCGCCGCAATCGGAAGCCCCACCATTCCCGTGCCGGGAACGCTGACGCCCAGACCGTTCTTCAACAGATTACGCTAAACCCACGCGTCTACACGTTAAAACGGACCGTCCAGATCTGCCGCAGCAAccgccg                                                           >  2:33797/1‑150 (MQ=255)
                                                                                             aCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGACCGGTGACGCCCAGACCGTTCTTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCACccgccgc                                                          >  2:393880/1‑150 (MQ=255)
                                                                                              ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTAAACGTTCAACCGGACCTTCCAGTTAAGCCGCAGCAAccgccgcc                                                         >  2:170302/1‑150 (MQ=255)
                                                                                               cTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgccg                                                        <  2:411980/150‑1 (MQ=255)
                                                                                                tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTTCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAGCCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                       >  1:379/1‑150 (MQ=255)
                                                                                                    gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                                   >  2:1467554/1‑150 (MQ=255)
                                                                                                     cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                                  >  2:535399/1‑150 (MQ=255)
                                                                                                       cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                                <  2:1858495/150‑1 (MQ=255)
                                                                                                               cgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTTCTTCAGCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGTCCTTCCAGTTCTGGCGCAGCAACCGCCGCCGCCAGCGCGAGTGAAAt                                        >  2:754126/1‑150 (MQ=255)
                                                                                                                 ccgcAATCCGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                                      <  1:1661255/150‑1 (MQ=255)
                                                                                                                  cgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                                     >  2:472470/1‑150 (MQ=255)
                                                                                                                   gcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGt                                    <  1:1636308/150‑1 (MQ=255)
                                                                                                                      aTCTGCAGCCCGAACATTCCCGTGACTGGAACGGTGACTCCCAGACGGTTCTTCAAGAGTTTTGGCGAAACCCAGGCTTCTACACGTTCAACCTGATCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCa                                 <  1:301416/150‑1 (MQ=255)
                                                                                                                         ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTAAGCCGCAGCAACCGCCGCCGCCAGCGCCAGAGAAAACGGTTCAGTa                              >  2:1763988/1‑150 (MQ=255)
                                                                                                                         ggCAGCCCCAACATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTGCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTa                              >  1:1227323/1‑150 (MQ=255)
                                                                                                                          gCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTAc                             >  2:1306282/1‑150 (MQ=255)
                                                                                                                             gCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCAGTGAAATCGGGTCAGTACATc                          >  1:667389/1‑150 (MQ=255)
                                                                                                                              ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACCTcc                         >  2:1232468/1‑150 (MQ=255)
                                                                                                                                ccaccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCGGCGCCAGTGAAATCGGTTCAGTACATcccc                       >  1:472698/1‑149 (MQ=255)
                                                                                                                                 caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACGTCCCAg                      >  2:370791/1‑150 (MQ=255)
                                                                                                                                 caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg                      >  1:1781234/1‑150 (MQ=255)
                                                                                                                                  accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc                     >  2:1066377/1‑150 (MQ=255)
                                                                                                                                  accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc                     >  1:430506/1‑150 (MQ=255)
                                                                                                                                     aTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc                  <  2:986535/150‑1 (MQ=255)
                                                                                                                                      ttCCCGTGCCGGGACCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCCGTGAAATCGGTTCAGTACATCCCCGCGCCg                 >  2:1315159/1‑150 (MQ=255)
                                                                                                                                       tCCCGCGCCGGGAACGGTGACGCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAACGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCgg                <  2:670327/150‑1 (MQ=255)
                                                                                                                                         ccgggccgggAACGGTGACGCCCAGCGCGGTCTTCATCAGATTTGGCGACACCCATGCTTCGAAACGTTCAACCGGAGCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGAGACAGTGAAATCGGTTAAGTACATCCCAGCGCCTGtt              <  2:317715/150‑1 (MQ=255)
                                                                                                                                           gTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt            <  2:34721/150‑1 (MQ=255)
                                                                                                                                           gTGCCGGGAAAGGTGACGCACAGACCGTTCTTCATCAGATTTGGCGAAAACCAGTATTCTACACGGTCAACCGGTCCTTCCTGTGCTGCCGCAGCTACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtttt            <  2:107306/150‑1 (MQ=255)
                                                                                                                                             gCCGGGTACGGGGACGCCCAGACCGTTATTCATCAGATTTGGTGAACCCCAGGCTTCTACACGATCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc          <  2:973489/150‑1 (MQ=255)
                                                                                                                                             gCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTAc          <  2:517400/150‑1 (MQ=255)
                                                                                                                                              ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGACACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt         <  1:1373172/150‑1 (MQ=255)
                                                                                                                                              ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt         <  2:1284836/150‑1 (MQ=255)
                                                                                                                                              ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt         <  1:1618487/150‑1 (MQ=255)
                                                                                                                                                 ggAACGGTGACTCCCATACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACAAGTTCAACCGGACCTTCCTGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcc      <  2:1359565/150‑1 (MQ=255)
                                                                                                                                                 ggAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACACGTGCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcc      >  1:207877/1‑150 (MQ=255)
                                                                                                                                                 ggAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcc      >  2:1537491/1‑150 (MQ=255)
                                                                                                                                                    aCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcctcc   <  2:1090734/150‑1 (MQ=255)
                                                                                                                                                     cGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTcctcct  <  1:1662896/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCT  >  NC_000913/3256301‑3256599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: